Click here to switch to the map view.

The map label for this gene is hydH [H]

Identifier: 189352116

GI number: 189352116

Start: 142520

End: 143827

Strand: Reverse

Name: hydH [H]

Synonym: BMULJ_03334

Alternate gene names: 189352116

Gene position: 143827-142520 (Counterclockwise)

Preceding gene: 189352126

Following gene: 189352111

Centisome position: 5.82

GC content: 66.21

Gene sequence:

>1308_bases
ATGCCGCACTTCCGTCATTCGGCTTCGCGCGCAGCGCGTAAGCTTCAGCTCGAGCACTTGGTTCCCATGTCGACCTCCGC
CACGTTCGCGCCCGACCCGCTCGCCGCCGCCCGTTCGGCGGAAAGCGAGCTGTTCCTGTCCGCACCGATTCCGATCCTGA
TCGAGGACTGGTCGCGCGTCTATCGCGCGATCAAGGACCTGCGCGCATCCGGCATCGACAATATCGACTGCTATTTCGAC
GAACATCCGGAGGCCGTCGCGCAGCTTCGCGCGCAGCATTCGTTCGTCGCTGCGAACGATGCCGTCGTCCGCCTGTTCGA
CGCCGATTCGAGGGAACACTTCCTGCAGCACGCGCGGATCCTGCTGCCGGCCGATCGCCTCAGCAACAGCGCCGTGCTTC
GCGCGATGTACGAGAATCGGCCGACCTGCCAGGGCGAGCGCACGCTCACGACGCTCAAAGGGCGCACCGTGCCGATCGTG
TGGCGCTGCTCGCTGCCGAAGGACGAGGACGGCTATCGCCGCCTGCATTTCTACGCATTCGACGTGACCGAACAGAAGGA
AAACAGCGACAGGCTCGAGGCGCTGCGCGCGGAAATGGCGCGGGCGTCGCGCGTTTCGCTGTTCGGCGAGATGTCGGCGT
CGATCCTGCATGAAGTCAGCCAGCCGCTGTCGGCGGCGAGCGCCTCGGCCGAAGCGTCGTTGCGCTGGCTCGCGCGCGAC
GAGCCCAACATCGCGGAAGCGACGGCCGCCATCGCCAACGCGGCGCGCTGGGCGCGCGACGCCACCGATATTTGCCGAAA
AATTCGCAGCTTCATCGGCAAGGCGCCGGTCCAGTCCATCGACTGCCCTGCGGCCGATGTCGTCACCGCCGCGCTGTTCC
TGATTGCCCCGGAAGCCGGTGCGAGAGACGTTCATGTCGAGACCGCCGTCGATCCTGACGCACGCATCTTCGCCGATCCG
GTGCAAATCCAGCAGGTGCTGGCAAATCTGCTGCTGAACGGCATCCAGGCAATCGACATGGGCCCGTCACGCGCACGCAC
GCTCACGTTGACCGTCACGGTGAACGGAAACGAAGTCGTGTTCGACGTGCGCGATACGGGGCCGGGCATCGACCCGGCTG
CGATCGAGCAGCTTTTTCAGCCGTTTTTCACGAGCAAACGCGACGGCATGGGCATGGGCCTGCCCGTCGCACGCTCGATC
GTCGAGGCGCACAACGGACGGATCTGGGCACACGGCGAACGCGGCGCCGGCTGCTGCTTCTCGTTCGCGCTCCCCGCGCG
CAGCGGCAGGCCCGATCCGGCCGAATGA

Upstream 100 bases:

>100_bases
ATGAAGGCGCAGCGCAAGCGACGGCAAGCAGCCACCGCTCAATCTGGTTATAGTGGCAGCTGTATCGAGCGCGGGCCGCA
GCCCATCGTCCGTTGCTTCG

Downstream 100 bases:

>100_bases
CCGCAGCGCGGCGCGGCGTCCCGCGTGCAGCGCATACCGCGCACGTGCAAGCGGATTGCCGCTCCGCGTGACGAGCTGCC
GCTAGCAACCGGCCGACAGC

Product: two-component system sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MPHFRHSASRAARKLQLEHLVPMSTSATFAPDPLAAARSAESELFLSAPIPILIEDWSRVYRAIKDLRASGIDNIDCYFD
EHPEAVAQLRAQHSFVAANDAVVRLFDADSREHFLQHARILLPADRLSNSAVLRAMYENRPTCQGERTLTTLKGRTVPIV
WRCSLPKDEDGYRRLHFYAFDVTEQKENSDRLEALRAEMARASRVSLFGEMSASILHEVSQPLSAASASAEASLRWLARD
EPNIAEATAAIANAARWARDATDICRKIRSFIGKAPVQSIDCPAADVVTAALFLIAPEAGARDVHVETAVDPDARIFADP
VQIQQVLANLLLNGIQAIDMGPSRARTLTLTVTVNGNEVVFDVRDTGPGIDPAAIEQLFQPFFTSKRDGMGMGLPVARSI
VEAHNGRIWAHGERGAGCCFSFALPARSGRPDPAE

Sequences:

>Translated_435_residues
MPHFRHSASRAARKLQLEHLVPMSTSATFAPDPLAAARSAESELFLSAPIPILIEDWSRVYRAIKDLRASGIDNIDCYFD
EHPEAVAQLRAQHSFVAANDAVVRLFDADSREHFLQHARILLPADRLSNSAVLRAMYENRPTCQGERTLTTLKGRTVPIV
WRCSLPKDEDGYRRLHFYAFDVTEQKENSDRLEALRAEMARASRVSLFGEMSASILHEVSQPLSAASASAEASLRWLARD
EPNIAEATAAIANAARWARDATDICRKIRSFIGKAPVQSIDCPAADVVTAALFLIAPEAGARDVHVETAVDPDARIFADP
VQIQQVLANLLLNGIQAIDMGPSRARTLTLTVTVNGNEVVFDVRDTGPGIDPAAIEQLFQPFFTSKRDGMGMGLPVARSI
VEAHNGRIWAHGERGAGCCFSFALPARSGRPDPAE
>Mature_434_residues
PHFRHSASRAARKLQLEHLVPMSTSATFAPDPLAAARSAESELFLSAPIPILIEDWSRVYRAIKDLRASGIDNIDCYFDE
HPEAVAQLRAQHSFVAANDAVVRLFDADSREHFLQHARILLPADRLSNSAVLRAMYENRPTCQGERTLTTLKGRTVPIVW
RCSLPKDEDGYRRLHFYAFDVTEQKENSDRLEALRAEMARASRVSLFGEMSASILHEVSQPLSAASASAEASLRWLARDE
PNIAEATAAIANAARWARDATDICRKIRSFIGKAPVQSIDCPAADVVTAALFLIAPEAGARDVHVETAVDPDARIFADPV
QIQQVLANLLLNGIQAIDMGPSRARTLTLTVTVNGNEVVFDVRDTGPGIDPAAIEQLFQPFFTSKRDGMGMGLPVARSIV
EAHNGRIWAHGERGAGCCFSFALPARSGRPDPAE

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=245, Percent_Identity=31.0204081632653, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788549, Length=246, Percent_Identity=28.0487804878049, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87081816, Length=145, Percent_Identity=32.4137931034483, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790300, Length=228, Percent_Identity=32.0175438596491, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693157, Length=124, Percent_Identity=33.0645161290323, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786912, Length=241, Percent_Identity=26.5560165975104, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789149, Length=257, Percent_Identity=22.568093385214, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788393, Length=225, Percent_Identity=24.8888888888889, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47636; Mature: 47505

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHFRHSASRAARKLQLEHLVPMSTSATFAPDPLAAARSAESELFLSAPIPILIEDWSRV
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCCHHHHHHHHH
YRAIKDLRASGIDNIDCYFDEHPEAVAQLRAQHSFVAANDAVVRLFDADSREHFLQHARI
HHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCEEECCCEEEEEECCCCHHHHHHHHHH
LLPADRLSNSAVLRAMYENRPTCQGERTLTTLKGRTVPIVWRCSLPKDEDGYRRLHFYAF
EEEHHHCCCHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEEEECCCCCCCCCEEEEEEEE
DVTEQKENSDRLEALRAEMARASRVSLFGEMSASILHEVSQPLSAASASAEASLRWLARD
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEECC
EPNIAEATAAIANAARWARDATDICRKIRSFIGKAPVQSIDCPAADVVTAALFLIAPEAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCC
ARDVHVETAVDPDARIFADPVQIQQVLANLLLNGIQAIDMGPSRARTLTLTVTVNGNEVV
CCEEEEEECCCCCCCEEECHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEEEECCCEEE
FDVRDTGPGIDPAAIEQLFQPFFTSKRDGMGMGLPVARSIVEAHNGRIWAHGERGAGCCF
EEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCEEE
SFALPARSGRPDPAE
EEECCCCCCCCCCCC
>Mature Secondary Structure 
PHFRHSASRAARKLQLEHLVPMSTSATFAPDPLAAARSAESELFLSAPIPILIEDWSRV
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCCHHHHHHHHH
YRAIKDLRASGIDNIDCYFDEHPEAVAQLRAQHSFVAANDAVVRLFDADSREHFLQHARI
HHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCEEECCCEEEEEECCCCHHHHHHHHHH
LLPADRLSNSAVLRAMYENRPTCQGERTLTTLKGRTVPIVWRCSLPKDEDGYRRLHFYAF
EEEHHHCCCHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEEEECCCCCCCCCEEEEEEEE
DVTEQKENSDRLEALRAEMARASRVSLFGEMSASILHEVSQPLSAASASAEASLRWLARD
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEECC
EPNIAEATAAIANAARWARDATDICRKIRSFIGKAPVQSIDCPAADVVTAALFLIAPEAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCC
ARDVHVETAVDPDARIFADPVQIQQVLANLLLNGIQAIDMGPSRARTLTLTVTVNGNEVV
CCEEEEEECCCCCCCEEECHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEEEECCCEEE
FDVRDTGPGIDPAAIEQLFQPFFTSKRDGMGMGLPVARSIVEAHNGRIWAHGERGAGCCF
EEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCEEE
SFALPARSGRPDPAE
EEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]