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The map label for this gene is iscS [H]

Identifier: 189352111

GI number: 189352111

Start: 136149

End: 137627

Strand: Reverse

Name: iscS [H]

Synonym: BMULJ_03329

Alternate gene names: 189352111

Gene position: 137627-136149 (Counterclockwise)

Preceding gene: 189352116

Following gene: 189352107

Centisome position: 5.56

GC content: 69.17

Gene sequence:

>1479_bases
ATGAGCGACAGCACCCCGTTCCATTACCTCGACTACGCCGCCACCACGCCCGCCGATCCCCGCGTCATTGAAGCGATGAC
CACCTGCCTCGGCTTCGACGGCATCTTCGGCAATCCGGCCTCCAGTTCGCACAACGCAGGCCGCCTCGCAAGGCAGAAAG
TCGAGCACGCGCGCAAACAGGTCGCCGCGCTGATCGGCGCACACGCAGACGAAATCGTCTGGACGTCGGGTGCCACCGAA
TCGAACAACCTCGCGCTGAAGGGTTACGCGGACACCGCGACGGAGCGGCGCCACCTGATCACGAGCCGCATCGAGCACAA
GGCGATCCTCGACACGATGGCGAACCTGTCGAAGCGCGGGATGACGGTCACCTGGCTGTCGCCCACGGCGGACGGCGAAA
TCACGGCCGACGCCGTCGCCGCGGCGATCGGCCCCGATACCGGTCTCGTCTCGCTGATGCTCGTGAACAACGAACTCGGC
ACGCTGACCGACGTCGGCGCGATCGCGCGCATCGTCCACGCGGCGGGCGCGCTGCTGCACGTCGATGCCGCGCAGGCGCT
CGGCAAGACGCCGATCGACGTGCACGCGCTCGGCATCGACATGATGTCGATGTCCGCACACAAGGTTTACGGTCCCAAGG
GTATCGGCGCGCTGTACGTGCGCCGCGACATCGCCGAGCGGATCGCGCCGCAGATACATGGCGGCGGCCACGAGCGCGGG
CTGCGCTCGGGCACGCTCGCGACGCATCAGATCGTCGGCATGGGCGTCGCCTGCGAGCTCGCGGCAGCCAAGCTCGACAG
CGAAGCCGCGCGCATTGCGGCGCTCGCCACGCGCCTGAAGGATGCCGTGCTCGCACTCGGCGACGTCACGCACAACGCGG
CCGCCGCCGCGCGCATTCCGCACACGCTGAGTCTGACGGTCGACGCACCGGGCTTCTTGCCGTTCATGCTGGGCGACACG
CTGGCGGTGTCGTCGACGTCCGCATGCAATTCGACGAGCGGCGCACCTTCGCATGTGCTGACCGCGATCGGCCTCGATGC
GGGCGCCGCGAGCCGCACCGTGCGCGTCAGCCTGGGCCGCTTCACGACCGAGCAGGACATCGACGCCGCGATCGCGTGCT
TCCGGCGCGCGATCGAACAGTGCCGCGCCGCCGCATCGAGCGGCTTCGCCGCGTCGCGCCAGATCACGCCGGCCGACCTG
CGCGCGATCCGCGACGCCGGCTATCGCGCCGTCATCTGCAACCGGCCGGACGGCGAAGACGCCGATCAGCCCGCGTTCGA
GGAAATCGCGGCAGCGGCACGGGAACTGGGACTCGATGCACGCTATCTGCCGGTAGGTCGCGACGAAATCGGCGACGCGG
ACGTCGAAGCGTTCGCCGCACTCGTCGACGCGCTGCCGAAACCGGTGCTCGCGTATTGCCGAAGCGGCAGCCGCGCCGGG
ATGCTGTGGCATCGACTGACGATGCGCAGCGCCGCGTAA

Upstream 100 bases:

>100_bases
TTCAGTTTCCACTTCTACGCCGTTGCACGCCTCATTTTGGAGCCATTTTCCGCCGCGCGCGCCGTATCATTTTCGCCGTT
CCGACGCGCAAGAGAAAACC

Downstream 100 bases:

>100_bases
TCGCACACATTCGGCACGTCGCGCGCGGCGCGCTGCTCAAACGGCGACCGGACGCATCCGGTCGCCGCCATCGCTACCTC
ACTCCTGCTGCTCCTGTTGC

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSDSTPFHYLDYAATTPADPRVIEAMTTCLGFDGIFGNPASSSHNAGRLARQKVEHARKQVAALIGAHADEIVWTSGATE
SNNLALKGYADTATERRHLITSRIEHKAILDTMANLSKRGMTVTWLSPTADGEITADAVAAAIGPDTGLVSLMLVNNELG
TLTDVGAIARIVHAAGALLHVDAAQALGKTPIDVHALGIDMMSMSAHKVYGPKGIGALYVRRDIAERIAPQIHGGGHERG
LRSGTLATHQIVGMGVACELAAAKLDSEAARIAALATRLKDAVLALGDVTHNAAAAARIPHTLSLTVDAPGFLPFMLGDT
LAVSSTSACNSTSGAPSHVLTAIGLDAGAASRTVRVSLGRFTTEQDIDAAIACFRRAIEQCRAAASSGFAASRQITPADL
RAIRDAGYRAVICNRPDGEDADQPAFEEIAAAARELGLDARYLPVGRDEIGDADVEAFAALVDALPKPVLAYCRSGSRAG
MLWHRLTMRSAA

Sequences:

>Translated_492_residues
MSDSTPFHYLDYAATTPADPRVIEAMTTCLGFDGIFGNPASSSHNAGRLARQKVEHARKQVAALIGAHADEIVWTSGATE
SNNLALKGYADTATERRHLITSRIEHKAILDTMANLSKRGMTVTWLSPTADGEITADAVAAAIGPDTGLVSLMLVNNELG
TLTDVGAIARIVHAAGALLHVDAAQALGKTPIDVHALGIDMMSMSAHKVYGPKGIGALYVRRDIAERIAPQIHGGGHERG
LRSGTLATHQIVGMGVACELAAAKLDSEAARIAALATRLKDAVLALGDVTHNAAAAARIPHTLSLTVDAPGFLPFMLGDT
LAVSSTSACNSTSGAPSHVLTAIGLDAGAASRTVRVSLGRFTTEQDIDAAIACFRRAIEQCRAAASSGFAASRQITPADL
RAIRDAGYRAVICNRPDGEDADQPAFEEIAAAARELGLDARYLPVGRDEIGDADVEAFAALVDALPKPVLAYCRSGSRAG
MLWHRLTMRSAA
>Mature_491_residues
SDSTPFHYLDYAATTPADPRVIEAMTTCLGFDGIFGNPASSSHNAGRLARQKVEHARKQVAALIGAHADEIVWTSGATES
NNLALKGYADTATERRHLITSRIEHKAILDTMANLSKRGMTVTWLSPTADGEITADAVAAAIGPDTGLVSLMLVNNELGT
LTDVGAIARIVHAAGALLHVDAAQALGKTPIDVHALGIDMMSMSAHKVYGPKGIGALYVRRDIAERIAPQIHGGGHERGL
RSGTLATHQIVGMGVACELAAAKLDSEAARIAALATRLKDAVLALGDVTHNAAAAARIPHTLSLTVDAPGFLPFMLGDTL
AVSSTSACNSTSGAPSHVLTAIGLDAGAASRTVRVSLGRFTTEQDIDAAIACFRRAIEQCRAAASSGFAASRQITPADLR
AIRDAGYRAVICNRPDGEDADQPAFEEIAAAARELGLDARYLPVGRDEIGDADVEAFAALVDALPKPVLAYCRSGSRAGM
LWHRLTMRSAA

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=379, Percent_Identity=43.7994722955145, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI156713448, Length=413, Percent_Identity=33.4140435835351, Blast_Score=199, Evalue=5e-51,
Organism=Escherichia coli, GI48994898, Length=376, Percent_Identity=49.468085106383, Blast_Score=355, Evalue=5e-99,
Organism=Escherichia coli, GI1789175, Length=393, Percent_Identity=29.5165394402036, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1787970, Length=281, Percent_Identity=28.8256227758007, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25143064, Length=374, Percent_Identity=42.7807486631016, Blast_Score=280, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17533177, Length=320, Percent_Identity=33.75, Blast_Score=142, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6319831, Length=407, Percent_Identity=40.2948402948403, Blast_Score=285, Evalue=2e-77,
Organism=Drosophila melanogaster, GI20129463, Length=380, Percent_Identity=45, Blast_Score=293, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 51385; Mature: 51254

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSTPFHYLDYAATTPADPRVIEAMTTCLGFDGIFGNPASSSHNAGRLARQKVEHARKQ
CCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VAALIGAHADEIVWTSGATESNNLALKGYADTATERRHLITSRIEHKAILDTMANLSKRG
HHHHHHCCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MTVTWLSPTADGEITADAVAAAIGPDTGLVSLMLVNNELGTLTDVGAIARIVHAAGALLH
CEEEEECCCCCCCEEHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCHHEE
VDAAQALGKTPIDVHALGIDMMSMSAHKVYGPKGIGALYVRRDIAERIAPQIHGGGHERG
EEHHHHHCCCCCEEEEECHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHCHHCCCCCHHCC
LRSGTLATHQIVGMGVACELAAAKLDSEAARIAALATRLKDAVLALGDVTHNAAAAARIP
CCCCCCHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCC
HTLSLTVDAPGFLPFMLGDTLAVSSTSACNSTSGAPSHVLTAIGLDAGAASRTVRVSLGR
CEEEEEECCCCCHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECC
FTTEQDIDAAIACFRRAIEQCRAAASSGFAASRQITPADLRAIRDAGYRAVICNRPDGED
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCC
ADQPAFEEIAAAARELGLDARYLPVGRDEIGDADVEAFAALVDALPKPVLAYCRSGSRAG
CCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCH
MLWHRLTMRSAA
HHHHHHHHHCCC
>Mature Secondary Structure 
SDSTPFHYLDYAATTPADPRVIEAMTTCLGFDGIFGNPASSSHNAGRLARQKVEHARKQ
CCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VAALIGAHADEIVWTSGATESNNLALKGYADTATERRHLITSRIEHKAILDTMANLSKRG
HHHHHHCCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MTVTWLSPTADGEITADAVAAAIGPDTGLVSLMLVNNELGTLTDVGAIARIVHAAGALLH
CEEEEECCCCCCCEEHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCHHEE
VDAAQALGKTPIDVHALGIDMMSMSAHKVYGPKGIGALYVRRDIAERIAPQIHGGGHERG
EEHHHHHCCCCCEEEEECHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHCHHCCCCCHHCC
LRSGTLATHQIVGMGVACELAAAKLDSEAARIAALATRLKDAVLALGDVTHNAAAAARIP
CCCCCCHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCC
HTLSLTVDAPGFLPFMLGDTLAVSSTSACNSTSGAPSHVLTAIGLDAGAASRTVRVSLGR
CEEEEEECCCCCHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECC
FTTEQDIDAAIACFRRAIEQCRAAASSGFAASRQITPADLRAIRDAGYRAVICNRPDGED
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCC
ADQPAFEEIAAAARELGLDARYLPVGRDEIGDADVEAFAALVDALPKPVLAYCRSGSRAG
CCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCH
MLWHRLTMRSAA
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA