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The map label for this gene is traD [H]

Identifier: 189184739

GI number: 189184739

Start: 1863476

End: 1865230

Strand: Direct

Name: traD [H]

Synonym: OTT_1832

Alternate gene names: 189184739

Gene position: 1863476-1865230 (Clockwise)

Preceding gene: 189184738

Following gene: 189184744

Centisome position: 92.76

GC content: 32.88

Gene sequence:

>1755_bases
ATGAATTTTCAGAATCAAGGCAATTTCACAAGGGGATCGCAGTTATTTGCCCATAAGTTAAGAATGTTTGGGCAAGGTAG
TACCAACGTTTTTATAATTGGATTAGGATTATCGATATTCTGGATTATATGTCGATTATATCAAAAAGTTTTTCTGAGCA
GCTTGTATTATTTTGCAATTGAAAGATATGTGCAGCTTAAGTTAGCAATTGGTGAACATTTTTATGATATCGATCAAATA
GGCATTAAGTTTTATAGTCTAAGGTTTAAAAAATGGATACACCTCAATGCTCAAGACTTTTTGCATGAGTTTTATACAGG
CCAACAGGGATTTAAAATACAGCAATTATGTGAATTCTTAATCAATTCAGCATTACTAGAAGGATTAATTATTTTTACTA
TTGGTGTGATAATCTCAATTGTTTTCTTTACGGCTCAAGGTAAAAAAATGATTATTAAGGCCAAAATTAGAGGTGCTGAT
TTTGTGGAGTATAAATTCCTAGCTAAAATGCTAAAAAGGTCTAAAAAGGCCTCGAAAATCTGTTTTGGAGGCTTGCCATT
AGTAAAGAATAGTGAAAGGCTGCACATTCTTATTACTGGAACAACAGGTACTGGTAAAACTAATATGCTTAATGAACTGC
TACCACAAATTCGATTGCAACAGGATCGAGCAATAATTGTAGACGTTACTGGAGCTTTTACTGATAGATTTTTTGATTCT
AAATGTGATAAGCTGCTTAATCCTTTGGAAAAAAATAGTGAGCAATGGTTGCCTTGGAATGATTGTTTTGAAGCAGCTGA
TTTTCATGATATAGCTAGTAGTTTTAGTAATTATAATCCTAGAATCGATGACTTTTTTGCTAAAAATGCTGAATTAGTCT
TATCTGAAGCGTTGAAGCTATATCAGGATGACAAAGATATCATAAAATTAATTCATACAATCATTTACTCTGATAATAGA
CAATTTGTAAAAGCATTTAGAAACACCGCTGTATCAGGCATTATAAGCGAAAGCGCGCCTGAAACTTCTGCAGGAATTCA
ATCTACTCTTGGAAAGAATATTACTTCGCTACAATATTTAAAGCCTGGAGGTAATTTTAGCATCAAGGAATGGTTTAGTA
ATTCTGCTGAAACTGGATGGCTATTTATCACAGCTAATCCAAGTCAAAGAGCTACTTTACGCCCACTTATTTCAGCATGG
ATAAGCATAGCTATCAAGGCTTTGATGTGTAGAAATCCTAATCATGATAACAAAAATATGTGGTTTATACTTGATGAACT
GCCAGCTCTACAAAAAGTTTCGTCTTTACCAATTGCTTTAGCTGAAAGTAGAAAGTATGGAGGCTGTTTTGTTGCTGGAT
TGCAGAACATTCATCAATTAGAAGAAATATATGGTTCTGCAGGATGTGCTTCTATGCTGGATTTGTTTAATAGTAAATTT
ATTTTTCGAGTTAGCGATCACGATACAGCTTATAAATCATCATTAATACTAGGGGAGGAAGAAATTATTGAAACTCAAGA
GAACTTGTCATATGGATCAAATACTATGCGAGATGGAGTAAATATGAATAATGTTGAGCGTAAAAAGCCTTTAGTTATAC
CATCTGAAATTATGAATCTACCAGACCTTACATGTTACGTAAAGCTTGCTGGTAACTTTCCCATTACAAAACTAACTATG
AAGCTACAAAACTTAAATATAGCATATGTTTGGGGATATAAATTGCTCAAAAAACTTAAGTTAGTGAATTATTAA

Upstream 100 bases:

>100_bases
CAGAACATGAAACAATGTTAAAAAGGTTAACAAAATTGAAGAATTTTACTGGAGTAAAAGAGTATGAGCAAAAACTACAG
ATATTGAAAAAATACTCTTG

Downstream 100 bases:

>100_bases
TTCAAGAGTTATCCATGCTAACTTTTAAATTAACCACTTCTTCGTTTGATAACCCAGTATTTTCAGAAATAAATTCAACT
GAAAAGCCAGCTTTTAATAA

Product: putative conjugative transfer protein TraD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MNFQNQGNFTRGSQLFAHKLRMFGQGSTNVFIIGLGLSIFWIICRLYQKVFLSSLYYFAIERYVQLKLAIGEHFYDIDQI
GIKFYSLRFKKWIHLNAQDFLHEFYTGQQGFKIQQLCEFLINSALLEGLIIFTIGVIISIVFFTAQGKKMIIKAKIRGAD
FVEYKFLAKMLKRSKKASKICFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRLQQDRAIIVDVTGAFTDRFFDS
KCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYNPRIDDFFAKNAELVLSEALKLYQDDKDIIKLIHTIIYSDNR
QFVKAFRNTAVSGIISESAPETSAGIQSTLGKNITSLQYLKPGGNFSIKEWFSNSAETGWLFITANPSQRATLRPLISAW
ISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPIALAESRKYGGCFVAGLQNIHQLEEIYGSAGCASMLDLFNSKF
IFRVSDHDTAYKSSLILGEEEIIETQENLSYGSNTMRDGVNMNNVERKKPLVIPSEIMNLPDLTCYVKLAGNFPITKLTM
KLQNLNIAYVWGYKLLKKLKLVNY

Sequences:

>Translated_584_residues
MNFQNQGNFTRGSQLFAHKLRMFGQGSTNVFIIGLGLSIFWIICRLYQKVFLSSLYYFAIERYVQLKLAIGEHFYDIDQI
GIKFYSLRFKKWIHLNAQDFLHEFYTGQQGFKIQQLCEFLINSALLEGLIIFTIGVIISIVFFTAQGKKMIIKAKIRGAD
FVEYKFLAKMLKRSKKASKICFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRLQQDRAIIVDVTGAFTDRFFDS
KCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYNPRIDDFFAKNAELVLSEALKLYQDDKDIIKLIHTIIYSDNR
QFVKAFRNTAVSGIISESAPETSAGIQSTLGKNITSLQYLKPGGNFSIKEWFSNSAETGWLFITANPSQRATLRPLISAW
ISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPIALAESRKYGGCFVAGLQNIHQLEEIYGSAGCASMLDLFNSKF
IFRVSDHDTAYKSSLILGEEEIIETQENLSYGSNTMRDGVNMNNVERKKPLVIPSEIMNLPDLTCYVKLAGNFPITKLTM
KLQNLNIAYVWGYKLLKKLKLVNY
>Mature_584_residues
MNFQNQGNFTRGSQLFAHKLRMFGQGSTNVFIIGLGLSIFWIICRLYQKVFLSSLYYFAIERYVQLKLAIGEHFYDIDQI
GIKFYSLRFKKWIHLNAQDFLHEFYTGQQGFKIQQLCEFLINSALLEGLIIFTIGVIISIVFFTAQGKKMIIKAKIRGAD
FVEYKFLAKMLKRSKKASKICFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRLQQDRAIIVDVTGAFTDRFFDS
KCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYNPRIDDFFAKNAELVLSEALKLYQDDKDIIKLIHTIIYSDNR
QFVKAFRNTAVSGIISESAPETSAGIQSTLGKNITSLQYLKPGGNFSIKEWFSNSAETGWLFITANPSQRATLRPLISAW
ISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPIALAESRKYGGCFVAGLQNIHQLEEIYGSAGCASMLDLFNSKF
IFRVSDHDTAYKSSLILGEEEIIETQENLSYGSNTMRDGVNMNNVERKKPLVIPSEIMNLPDLTCYVKLAGNFPITKLTM
KLQNLNIAYVWGYKLLKKLKLVNY

Specific function: TraD is a cell envelope protein. The function of traD is unknown but it is essential for DNA transfer. It may form or may be part of a non-specific pore for DNA export, or it may directly energizes DNA transport [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traD of plasmid F [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014128
- InterPro:   IPR019476
- InterPro:   IPR022585 [H]

Pfam domain/function: PF12615 TraD_N; PF10412 TrwB_AAD_bind [H]

EC number: NA

Molecular weight: Translated: 66465; Mature: 66465

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFQNQGNFTRGSQLFAHKLRMFGQGSTNVFIIGLGLSIFWIICRLYQKVFLSSLYYFAI
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
ERYVQLKLAIGEHFYDIDQIGIKFYSLRFKKWIHLNAQDFLHEFYTGQQGFKIQQLCEFL
HHHHHHHHHHCCHHHHHHHHHHHHHEEEEHHHEECCHHHHHHHHHCCCCCCCHHHHHHHH
INSALLEGLIIFTIGVIISIVFFTAQGKKMIIKAKIRGADFVEYKFLAKMLKRSKKASKI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCHHCCE
CFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRLQQDRAIIVDVTGAFTDRFFDS
EECCCCCEECCCEEEEEEEECCCCCCHHHHHHHHHHHEECCCCEEEEEECCHHHHHHHHH
KCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYNPRIDDFFAKNAELVLSEALKL
HHHHHHCHHHCCCCCCCCHHHHHHHCHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHH
YQDDKDIIKLIHTIIYSDNRQFVKAFRNTAVSGIISESAPETSAGIQSTLGKNITSLQYL
HCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEE
KPGGNFSIKEWFSNSAETGWLFITANPSQRATLRPLISAWISIAIKALMCRNPNHDNKNM
CCCCCCCHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
WFILDELPALQKVSSLPIALAESRKYGGCFVAGLQNIHQLEEIYGSAGCASMLDLFNSKF
EEEECCCCHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCE
IFRVSDHDTAYKSSLILGEEEIIETQENLSYGSNTMRDGVNMNNVERKKPLVIPSEIMNL
EEEECCCCCHHHHHEEECHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCHHHCCC
PDLTCYVKLAGNFPITKLTMKLQNLNIAYVWGYKLLKKLKLVNY
CCCEEEEEEECCCCHHHEEEEECCCCEEEEHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFQNQGNFTRGSQLFAHKLRMFGQGSTNVFIIGLGLSIFWIICRLYQKVFLSSLYYFAI
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
ERYVQLKLAIGEHFYDIDQIGIKFYSLRFKKWIHLNAQDFLHEFYTGQQGFKIQQLCEFL
HHHHHHHHHHCCHHHHHHHHHHHHHEEEEHHHEECCHHHHHHHHHCCCCCCCHHHHHHHH
INSALLEGLIIFTIGVIISIVFFTAQGKKMIIKAKIRGADFVEYKFLAKMLKRSKKASKI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCHHCCE
CFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRLQQDRAIIVDVTGAFTDRFFDS
EECCCCCEECCCEEEEEEEECCCCCCHHHHHHHHHHHEECCCCEEEEEECCHHHHHHHHH
KCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYNPRIDDFFAKNAELVLSEALKL
HHHHHHCHHHCCCCCCCCHHHHHHHCHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHH
YQDDKDIIKLIHTIIYSDNRQFVKAFRNTAVSGIISESAPETSAGIQSTLGKNITSLQYL
HCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEE
KPGGNFSIKEWFSNSAETGWLFITANPSQRATLRPLISAWISIAIKALMCRNPNHDNKNM
CCCCCCCHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
WFILDELPALQKVSSLPIALAESRKYGGCFVAGLQNIHQLEEIYGSAGCASMLDLFNSKF
EEEECCCCHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCE
IFRVSDHDTAYKSSLILGEEEIIETQENLSYGSNTMRDGVNMNNVERKKPLVIPSEIMNL
EEEECCCCCHHHHHEEECHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCHHHCCC
PDLTCYVKLAGNFPITKLTMKLQNLNIAYVWGYKLLKKLKLVNY
CCCEEEEEEECCCCHHHEEEEECCCCEEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2164585 [H]