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The map label for this gene is miaB [H]

Identifier: 189184684

GI number: 189184684

Start: 1802614

End: 1803963

Strand: Direct

Name: miaB [H]

Synonym: OTT_1777

Alternate gene names: 189184684

Gene position: 1802614-1803963 (Clockwise)

Preceding gene: 189184682

Following gene: 189184685

Centisome position: 89.73

GC content: 33.04

Gene sequence:

>1350_bases
ATGAATAAAATGTTGTATATAAAAACATATGGCTGTCAGATGAATGTTTATGACTCGAATCGTATGGTAGATCTACTAGA
AACTCAAGGCTATAATATTGTTGCAAATATGGCAGATGCTAGTGTAATAATATTAAATACTTGCCATATAAGGGAAAAAG
CATCAGAAAAGATGTACTCTGAATTAGGCAGAATTAAAAAATTGCAGCAGAGTCGGCTTAAAGCTGGTAAAAGTAAAGCT
AAAATAATAGTAGCAGGATGCGTTGGGCAGGCTGAAGGAGAAGAAATTTTTATTCGAGAGCCAGCAGTTAACATCATCGT
TGGCCCTCAGTCATATTATAATTTACCAACTATGCTTGAAAAATTAGATAGTGGTACAGAAAATCATTTAATCGATTTAG
ATTTTGTTGAAGCAGCTAAATTTAATAAACTTCCTGAAGTTTTAAAATCACCTACTGTGAGTGGCTTAGTATCTGTTCAA
GAAGGGTGTGATAAATTTTGTACATTTTGTGTTGTACCTTATACTAGAGGAGCTGAGTTTTCAAGACCTCTTGAACAAGT
CTATCGTGAAGTTCTTAATATCGCTCAACAAGGTGCTAAAGAAGTTGTATTAGTAGGACAAAATGTAAGCGCGTATCATG
GTAAAGATGAGAATGGTAAAGAATGTAGTTTAGCTGACCTAATTAAATATGTTGCTAAAATAGATAAAATTAAAAGAATT
AGATATATCACTTCTCATCCAAATGATATGACTGATCAGTTGTTATCTTTACATGCAACAGAAGAGAAGTTAATGCCTTT
TTTACATTTGCCTGTTCAATCTGGTTCTAATAAAATATTAAAGTTAATGAATAGAAGACATAGTAGAGAACGATATTTGG
AAATTATTCAACAACTAAGAGAATTAAGGCCTGATATAGTAATTTCTAGTGATATTATTGTAGGCTTTCCAGGAGAAGAT
GATGAGGATTTTGAAGCAACTTTGAGTTTAGCTAAAGAAGCTAGGTTTGGACAATGCTATTCCTTTAAATATAGTCAAAG
ACCAGGTACTCCTGCAGCCGTTAAACAGCAAATTTCCGAAGAAGTAAAACAGCATAGGTTAAGTATATTGCAGGCTCAGC
TAATGCAGCAGCAGCTAGAATGCAATCAAAAATTAATTGGTAAGGTAGTGCCAGTATTATTTGACCGTGATGGTAAGTAT
GACGGTCAAATAATCGGTAAGACACCTTATATGCAATCTGTTTGTATTATGAATGAGAAAGATAATAATCTATATGGTAA
AATAGTTAATGTAAAGATCTTGACTGCTTCAGCATCAAGTCTATTTGGAGAAGTTTATGCTGATAGTTAA

Upstream 100 bases:

>100_bases
TAAGGCAAGTATATTTTAAATTTGGTATAGTATTTTACATCAACATAGTTTTTTAATTAAATAATACAGATAAACTAGAG
AATTTAGTAGTTAAGTGATT

Downstream 100 bases:

>100_bases
AAAGTAACATATTATGCATAAAATTAACTTTTTAGTAACACAATTCAACACACTAACATAAGGCGTGGTCATTGAAAGCA
CTAATTTTCAACATATTTTA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MNKMLYIKTYGCQMNVYDSNRMVDLLETQGYNIVANMADASVIILNTCHIREKASEKMYSELGRIKKLQQSRLKAGKSKA
KIIVAGCVGQAEGEEIFIREPAVNIIVGPQSYYNLPTMLEKLDSGTENHLIDLDFVEAAKFNKLPEVLKSPTVSGLVSVQ
EGCDKFCTFCVVPYTRGAEFSRPLEQVYREVLNIAQQGAKEVVLVGQNVSAYHGKDENGKECSLADLIKYVAKIDKIKRI
RYITSHPNDMTDQLLSLHATEEKLMPFLHLPVQSGSNKILKLMNRRHSRERYLEIIQQLRELRPDIVISSDIIVGFPGED
DEDFEATLSLAKEARFGQCYSFKYSQRPGTPAAVKQQISEEVKQHRLSILQAQLMQQQLECNQKLIGKVVPVLFDRDGKY
DGQIIGKTPYMQSVCIMNEKDNNLYGKIVNVKILTASASSLFGEVYADS

Sequences:

>Translated_449_residues
MNKMLYIKTYGCQMNVYDSNRMVDLLETQGYNIVANMADASVIILNTCHIREKASEKMYSELGRIKKLQQSRLKAGKSKA
KIIVAGCVGQAEGEEIFIREPAVNIIVGPQSYYNLPTMLEKLDSGTENHLIDLDFVEAAKFNKLPEVLKSPTVSGLVSVQ
EGCDKFCTFCVVPYTRGAEFSRPLEQVYREVLNIAQQGAKEVVLVGQNVSAYHGKDENGKECSLADLIKYVAKIDKIKRI
RYITSHPNDMTDQLLSLHATEEKLMPFLHLPVQSGSNKILKLMNRRHSRERYLEIIQQLRELRPDIVISSDIIVGFPGED
DEDFEATLSLAKEARFGQCYSFKYSQRPGTPAAVKQQISEEVKQHRLSILQAQLMQQQLECNQKLIGKVVPVLFDRDGKY
DGQIIGKTPYMQSVCIMNEKDNNLYGKIVNVKILTASASSLFGEVYADS
>Mature_449_residues
MNKMLYIKTYGCQMNVYDSNRMVDLLETQGYNIVANMADASVIILNTCHIREKASEKMYSELGRIKKLQQSRLKAGKSKA
KIIVAGCVGQAEGEEIFIREPAVNIIVGPQSYYNLPTMLEKLDSGTENHLIDLDFVEAAKFNKLPEVLKSPTVSGLVSVQ
EGCDKFCTFCVVPYTRGAEFSRPLEQVYREVLNIAQQGAKEVVLVGQNVSAYHGKDENGKECSLADLIKYVAKIDKIKRI
RYITSHPNDMTDQLLSLHATEEKLMPFLHLPVQSGSNKILKLMNRRHSRERYLEIIQQLRELRPDIVISSDIIVGFPGED
DEDFEATLSLAKEARFGQCYSFKYSQRPGTPAAVKQQISEEVKQHRLSILQAQLMQQQLECNQKLIGKVVPVLFDRDGKY
DGQIIGKTPYMQSVCIMNEKDNNLYGKIVNVKILTASASSLFGEVYADS

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=479, Percent_Identity=34.6555323590814, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI28872782, Length=479, Percent_Identity=34.6555323590814, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI93277076, Length=452, Percent_Identity=27.212389380531, Blast_Score=169, Evalue=5e-42,
Organism=Escherichia coli, GI1786882, Length=449, Percent_Identity=44.7661469933185, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1787057, Length=456, Percent_Identity=32.8947368421053, Blast_Score=210, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17553146, Length=425, Percent_Identity=31.5294117647059, Blast_Score=219, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI71996771, Length=314, Percent_Identity=27.3885350318471, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21356207, Length=432, Percent_Identity=31.4814814814815, Blast_Score=234, Evalue=9e-62,
Organism=Drosophila melanogaster, GI19922432, Length=454, Percent_Identity=27.7533039647577, Blast_Score=159, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50581; Mature: 50581

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKMLYIKTYGCQMNVYDSNRMVDLLETQGYNIVANMADASVIILNTCHIREKASEKMYS
CCCEEEEEEECEEEEEECCCCEEEHHHCCCCEEEEECCCCEEEEEEEHHHHHHHHHHHHH
ELGRIKKLQQSRLKAGKSKAKIIVAGCVGQAEGEEIFIREPAVNIIVGPQSYYNLPTMLE
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEECCCEEEEECCHHHCCHHHHHH
KLDSGTENHLIDLDFVEAAKFNKLPEVLKSPTVSGLVSVQEGCDKFCTFCVVPYTRGAEF
HHCCCCCCCEEEEEHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCCH
SRPLEQVYREVLNIAQQGAKEVVLVGQNVSAYHGKDENGKECSLADLIKYVAKIDKIKRI
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RYITSHPNDMTDQLLSLHATEEKLMPFLHLPVQSGSNKILKLMNRRHSRERYLEIIQQLR
HHHHCCCCHHHHHHHHHHCCHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ELRPDIVISSDIIVGFPGEDDEDFEATLSLAKEARFGQCYSFKYSQRPGTPAAVKQQISE
HCCCCEEEECCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHH
EVKQHRLSILQAQLMQQQLECNQKLIGKVVPVLFDRDGKYDGQIIGKTPYMQSVCIMNEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHEEEECCC
DNNLYGKIVNVKILTASASSLFGEVYADS
CCCEEEEEEEEEEEEECHHHHHHHHHCCC
>Mature Secondary Structure
MNKMLYIKTYGCQMNVYDSNRMVDLLETQGYNIVANMADASVIILNTCHIREKASEKMYS
CCCEEEEEEECEEEEEECCCCEEEHHHCCCCEEEEECCCCEEEEEEEHHHHHHHHHHHHH
ELGRIKKLQQSRLKAGKSKAKIIVAGCVGQAEGEEIFIREPAVNIIVGPQSYYNLPTMLE
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEECCCEEEEECCHHHCCHHHHHH
KLDSGTENHLIDLDFVEAAKFNKLPEVLKSPTVSGLVSVQEGCDKFCTFCVVPYTRGAEF
HHCCCCCCCEEEEEHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCCH
SRPLEQVYREVLNIAQQGAKEVVLVGQNVSAYHGKDENGKECSLADLIKYVAKIDKIKRI
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RYITSHPNDMTDQLLSLHATEEKLMPFLHLPVQSGSNKILKLMNRRHSRERYLEIIQQLR
HHHHCCCCHHHHHHHHHHCCHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ELRPDIVISSDIIVGFPGEDDEDFEATLSLAKEARFGQCYSFKYSQRPGTPAAVKQQISE
HCCCCEEEECCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHH
EVKQHRLSILQAQLMQQQLECNQKLIGKVVPVLFDRDGKYDGQIIGKTPYMQSVCIMNEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHEEEECCC
DNNLYGKIVNVKILTASASSLFGEVYADS
CCCEEEEEEEEEEEEECHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA