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The map label for this gene is mccF [C]

Identifier: 189184304

GI number: 189184304

Start: 1430003

End: 1431001

Strand: Direct

Name: mccF [C]

Synonym: OTT_1397

Alternate gene names: 189184304

Gene position: 1430003-1431001 (Clockwise)

Preceding gene: 189184302

Following gene: 189184311

Centisome position: 71.18

GC content: 33.03

Gene sequence:

>999_bases
ATGCGTTTTATTATTCTTCTTTTTTCTTTTGTAGTTTCAAGTGTAAGCATTTGCCTTGCTATACCAGATACAATAATTGA
TGTCATAGCACCAAGTTCTAAAGGTAGTGAATTAAATCTACAATTAATAGAAGATTACTTAGAATCTATAGGTGTAAAAG
CTAATGTTCCAGAAAATATCTACAGTGATAATGAACCATTGTATTCTAATACAGATGAGTTTAGAGCTGAAAGTTTAGTT
AAAGCATTAACTAATCCGAAGAGTATAGCTATTTGGTGTATTAGAGGAGGAAGAGGAGCTAGTAGGTTAATTCCATATCT
GGAAAAGCTACCTGACGATGTAAAAAAGAAGATTGCGCAAAATAAAAACAGAAAAATTCTGATGGGATTTAGTGATATTA
GTGTTTTACATATTTATTTACAAAATAAGTATGATTGGAGCACTTTGCATTCTCCAATGCTAGAGCAGATAGTAGACAAT
AAAATCTCTCAGGATTCTATTGATAAATTAGAACTATTAATCTCAGGATACCGCAATAATATCAAATTCAATCTAAAAAT
GATTGATAATGGTATTGAATTAAATGATGGTATATTAGAATCAAAGGTTGTTGGCGGTTCTATATCATTAGTTAGAGCTA
GCATAGGCACATGTTGGCAAATAGATACAAAGGATAAAATCCTGTTTTTAGAAGAAGTAGAACCTGACCCAGCTCTACTA
GAGCTCAGACTAGAGCATTTAAAGCAAGCTCATATTTTAGATGAAGTACAAGCCGTCATTTTTGGCGATATACTATGCAC
CAATGATGAACAGTTATTAGAATTAGTCAAGCACAGATTTGCTCAAAGCGTAAACTTTCCTGTATTTACACTAAATGGCA
TTGGGCATGCTCGTACTAATTACCCTTTACCTCTTAATACTCGAGCTGTCATTAATCATATTAAAGGCAATCGTTTCTCT
ATGACAGTGGATATGCCAGCAGAGTGGCGTAGAAGATAA

Upstream 100 bases:

>100_bases
TACTAAACAATTTTAATGTAAGTGTATTTAGTTGCACGCTTTAAAATATTCAAAAATAATTTTTAAAGCTTAAAGATCAT
ATTTTAATAAAAATATTCCA

Downstream 100 bases:

>100_bases
AGTGTTGTACTATAGGGTTTTTGTTTTCTTTTAAAAGGAATTAGGTCAGTTATTCTATGATTACGTTCTTTTTGATATCA
TGATTTTGCTTTTATAGCTT

Product: microcin C7 self-immunity protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MRFIILLFSFVVSSVSICLAIPDTIIDVIAPSSKGSELNLQLIEDYLESIGVKANVPENIYSDNEPLYSNTDEFRAESLV
KALTNPKSIAIWCIRGGRGASRLIPYLEKLPDDVKKKIAQNKNRKILMGFSDISVLHIYLQNKYDWSTLHSPMLEQIVDN
KISQDSIDKLELLISGYRNNIKFNLKMIDNGIELNDGILESKVVGGSISLVRASIGTCWQIDTKDKILFLEEVEPDPALL
ELRLEHLKQAHILDEVQAVIFGDILCTNDEQLLELVKHRFAQSVNFPVFTLNGIGHARTNYPLPLNTRAVINHIKGNRFS
MTVDMPAEWRRR

Sequences:

>Translated_332_residues
MRFIILLFSFVVSSVSICLAIPDTIIDVIAPSSKGSELNLQLIEDYLESIGVKANVPENIYSDNEPLYSNTDEFRAESLV
KALTNPKSIAIWCIRGGRGASRLIPYLEKLPDDVKKKIAQNKNRKILMGFSDISVLHIYLQNKYDWSTLHSPMLEQIVDN
KISQDSIDKLELLISGYRNNIKFNLKMIDNGIELNDGILESKVVGGSISLVRASIGTCWQIDTKDKILFLEEVEPDPALL
ELRLEHLKQAHILDEVQAVIFGDILCTNDEQLLELVKHRFAQSVNFPVFTLNGIGHARTNYPLPLNTRAVINHIKGNRFS
MTVDMPAEWRRR
>Mature_332_residues
MRFIILLFSFVVSSVSICLAIPDTIIDVIAPSSKGSELNLQLIEDYLESIGVKANVPENIYSDNEPLYSNTDEFRAESLV
KALTNPKSIAIWCIRGGRGASRLIPYLEKLPDDVKKKIAQNKNRKILMGFSDISVLHIYLQNKYDWSTLHSPMLEQIVDN
KISQDSIDKLELLISGYRNNIKFNLKMIDNGIELNDGILESKVVGGSISLVRASIGTCWQIDTKDKILFLEEVEPDPALL
ELRLEHLKQAHILDEVQAVIFGDILCTNDEQLLELVKHRFAQSVNFPVFTLNGIGHARTNYPLPLNTRAVINHIKGNRFS
MTVDMPAEWRRR

Specific function: L,D-carboxypeptidase [C]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=260, Percent_Identity=25.7692307692308, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 37573; Mature: 37573

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFIILLFSFVVSSVSICLAIPDTIIDVIAPSSKGSELNLQLIEDYLESIGVKANVPENI
CHHHHHHHHHHHHHHHEEEECHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCCHHH
YSDNEPLYSNTDEFRAESLVKALTNPKSIAIWCIRGGRGASRLIPYLEKLPDDVKKKIAQ
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHC
NKNRKILMGFSDISVLHIYLQNKYDWSTLHSPMLEQIVDNKISQDSIDKLELLISGYRNN
CCCCEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
IKFNLKMIDNGIELNDGILESKVVGGSISLVRASIGTCWQIDTKDKILFLEEVEPDPALL
EEEEEEEECCCCCCCCCHHHHHCCCCCHHHHHHCCCCEEEECCCCCEEEEEECCCCHHHH
ELRLEHLKQAHILDEVQAVIFGDILCTNDEQLLELVKHRFAQSVNFPVFTLNGIGHARTN
HHHHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
YPLPLNTRAVINHIKGNRFSMTVDMPAEWRRR
CCCCCCHHHHHHHCCCCEEEEEEECCHHHHCC
>Mature Secondary Structure
MRFIILLFSFVVSSVSICLAIPDTIIDVIAPSSKGSELNLQLIEDYLESIGVKANVPENI
CHHHHHHHHHHHHHHHEEEECHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCCHHH
YSDNEPLYSNTDEFRAESLVKALTNPKSIAIWCIRGGRGASRLIPYLEKLPDDVKKKIAQ
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHC
NKNRKILMGFSDISVLHIYLQNKYDWSTLHSPMLEQIVDNKISQDSIDKLELLISGYRNN
CCCCEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
IKFNLKMIDNGIELNDGILESKVVGGSISLVRASIGTCWQIDTKDKILFLEEVEPDPALL
EEEEEEEECCCCCCCCCHHHHHCCCCCHHHHHHCCCCEEEECCCCCEEEEEECCCCHHHH
ELRLEHLKQAHILDEVQAVIFGDILCTNDEQLLELVKHRFAQSVNFPVFTLNGIGHARTN
HHHHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
YPLPLNTRAVINHIKGNRFSMTVDMPAEWRRR
CCCCCCHHHHHHHCCCCEEEEEEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]