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The map label for this gene is pnp [H]

Identifier: 189184267

GI number: 189184267

Start: 1388283

End: 1390493

Strand: Reverse

Name: pnp [H]

Synonym: OTT_1360

Alternate gene names: 189184267

Gene position: 1390493-1388283 (Counterclockwise)

Preceding gene: 189184268

Following gene: 189184262

Centisome position: 69.21

GC content: 32.43

Gene sequence:

>2211_bases
ATGTTTAATGAGATACTAAAAAAAGTTGATTGGCATGGTAATATGCTATCTCTAAGTACTGGCAAGATAGCGCGTAATGC
TGACGGAGCAGTTTTGGCATCAATGGGCAACACGTCAGTATTGTGTACAGTAGTTTTTGATAAAAACACAAAGAAGGATA
TTGATTTTTTTCCGCTAGGTGTTTATTACAGAGAAATGGCCTATGCCGCTGGTAAAATTCCAGGAGGCTTTATAAAAAAA
GAGGGCAAGTTTTCTGAATATGAAGTATTAGTATCAAGATTAATAGACCGTTCTATTCGTCCACTTTTTGATTCTAACTT
TCGTAATGATACTCAGGTTATTTGCACAGTGATGTCATATGATCCAAGATATAGCCCTGATATTTTAGCAATTATTGGCA
GTTCTGCTGCATTAGCAATTTCTGGAATTCCAATCGTAAAACCTATTGGAGCTGCTAGAGTTGGAATAGTTAATGATGAA
TTTATATTAAATCCTGTAATTCACGACAATACAGGAGTCAATGAGCTTGATTTAGTGGTTGCAGCTACTTTTGATTCAGT
TACTATGATAGAGGCTCAAGCTTGTGAAATTGATGAAGAAAAAATGTTAGCTGCTATAGAGTTTGGATATAAATCACTAA
AACCTGTAATTAACGCTATTGAGGAAATAAAATCAAGCATTAGAAAGGATATTTTTGAAGTTACAGCTAGGCCACATCTA
AGGTATAATGATGAAATACTTAAGCATTTTAGCTCTGATATTAAATCAGCACTTTTACTACAAACTAAGAATGAACGTAA
TCAACAGCTGCAATTAATACAGCAAAAAGTTGTTGATTATTTTTCCAGTGAAGCTAATGATGATGATGCTATTTTAAATA
TAGAAAAAGCTTTAGATGATGTAAAATCTAAAATTTTTAGAGATTTAGTATTACAAGACAAAACCAGAATTGGAAATAGA
GCTATAGATGAAATTAGGCCTATTATTTGTGAAGCCGGTTTATTTAATACAGTGCATGGATCTGCACTATTTACTCGTGG
TGATACTCAAAGCTTAGCAACAATTACATTAGGTAGTAGTACTGACGAACAAATAGTTGAGCAGTTAAATAAGTGTGAAA
GGCAGAATTTTTTACTAGATTATATCTTTTTACCTTATTCAGTTGGCGAAATTTCGCCACTAAGAGCTGCTAGTAGAAGA
GAAATAGGCCATGGCTGGTTAGCTAAAAAAGCTATTCAACTTGTTATTCCTAGCAAAGATGTATTTCCATATACTATTAG
AATCGTATCAGAAATTACTCAATCTGATGGATCTTCTTCAATGGCAACAGTATGTAGCGCTTCGCTTAGCTTAATGGAAG
CTGGAGTTCCAATAAAAACACATGTTGCAGGTATTGCTATGGGGCTAGTTTTAGGTGAGGGCAATAAGTTTGAGATTTTA
TCTGATATATCTGGTTGCGAAGATCATTTAGGAGATATGGATTTTAAAGTAGCAAGTACTAAAAACGGTATTACAGCTTT
GCAATTAGATATTAAAGTTCAAGGAATTAATTTATCTATGATCGAAAGCACCTTTCGACAAGCTAAAATTGGTATAAATC
ATATATTAAATGTTATGAATAATACTATTAGTTGCCCTAAATCAGAACTAAGTACATATGCACCAATGGTTCAAACATTA
GAAATTCAAAAAGAAAAAATTCGTGATGTTATTGGATTAGGCGGTAAAGTTATTAAAGAGCTTTGCAAAACTTTTGATGT
TGAAATTGATATTAGTGAAAACGGAGAAGTTAAGGTTTGGGGAAATGTAGGTGAAAATGTAAAAAAAGCAGTACAAAGTA
TTGAAAATATTGTGTTCGTTCCACAAATAGGTGATATCTTTGATGGTGAAGTAGTTAAGGTTATTGAATCTGGAGCATTT
ATAAAATATGTGACTGGTCGAGATGGATTCGTGCATATTAGCGAAATTAATGATACACATATAAAAGATATTAATGCACA
CGTAAAATTAGGAGACAAGGTAAAAGTCAAAATTATTGGAATTGATCATAAGAATCGTGTTAAATTAACTTTGAGAACAG
ATAAAGAACATTGCAAGAACAAAAATGAGCAGTATAATGATATTACTACAACAACAGGCAGTGTAAAGAAAAAGATTAAA
ATCGCTCCCAAAGAAGCAGCAGTAATTTCTAATAGGAAATACTTTGATTAA

Upstream 100 bases:

>100_bases
CAACTTCTTAAATTAATTAAAGTTCTATATAAAGTTAGAATAGAGAAGTATTTTAGAATATTTATAAAAGTTTTTAGGGG
TTTTTTAAGGATAACAAATT

Downstream 100 bases:

>100_bases
TAATAAGCAACACGTTAGGGGAGGGGAGTAATATTAGAAGTTTAGTCAGTTTTTTTCAAGAAATGTATGTTATGACATAC
TTTATACTTCATACTCCTTA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 736; Mature: 736

Protein sequence:

>736_residues
MFNEILKKVDWHGNMLSLSTGKIARNADGAVLASMGNTSVLCTVVFDKNTKKDIDFFPLGVYYREMAYAAGKIPGGFIKK
EGKFSEYEVLVSRLIDRSIRPLFDSNFRNDTQVICTVMSYDPRYSPDILAIIGSSAALAISGIPIVKPIGAARVGIVNDE
FILNPVIHDNTGVNELDLVVAATFDSVTMIEAQACEIDEEKMLAAIEFGYKSLKPVINAIEEIKSSIRKDIFEVTARPHL
RYNDEILKHFSSDIKSALLLQTKNERNQQLQLIQQKVVDYFSSEANDDDAILNIEKALDDVKSKIFRDLVLQDKTRIGNR
AIDEIRPIICEAGLFNTVHGSALFTRGDTQSLATITLGSSTDEQIVEQLNKCERQNFLLDYIFLPYSVGEISPLRAASRR
EIGHGWLAKKAIQLVIPSKDVFPYTIRIVSEITQSDGSSSMATVCSASLSLMEAGVPIKTHVAGIAMGLVLGEGNKFEIL
SDISGCEDHLGDMDFKVASTKNGITALQLDIKVQGINLSMIESTFRQAKIGINHILNVMNNTISCPKSELSTYAPMVQTL
EIQKEKIRDVIGLGGKVIKELCKTFDVEIDISENGEVKVWGNVGENVKKAVQSIENIVFVPQIGDIFDGEVVKVIESGAF
IKYVTGRDGFVHISEINDTHIKDINAHVKLGDKVKVKIIGIDHKNRVKLTLRTDKEHCKNKNEQYNDITTTTGSVKKKIK
IAPKEAAVISNRKYFD

Sequences:

>Translated_736_residues
MFNEILKKVDWHGNMLSLSTGKIARNADGAVLASMGNTSVLCTVVFDKNTKKDIDFFPLGVYYREMAYAAGKIPGGFIKK
EGKFSEYEVLVSRLIDRSIRPLFDSNFRNDTQVICTVMSYDPRYSPDILAIIGSSAALAISGIPIVKPIGAARVGIVNDE
FILNPVIHDNTGVNELDLVVAATFDSVTMIEAQACEIDEEKMLAAIEFGYKSLKPVINAIEEIKSSIRKDIFEVTARPHL
RYNDEILKHFSSDIKSALLLQTKNERNQQLQLIQQKVVDYFSSEANDDDAILNIEKALDDVKSKIFRDLVLQDKTRIGNR
AIDEIRPIICEAGLFNTVHGSALFTRGDTQSLATITLGSSTDEQIVEQLNKCERQNFLLDYIFLPYSVGEISPLRAASRR
EIGHGWLAKKAIQLVIPSKDVFPYTIRIVSEITQSDGSSSMATVCSASLSLMEAGVPIKTHVAGIAMGLVLGEGNKFEIL
SDISGCEDHLGDMDFKVASTKNGITALQLDIKVQGINLSMIESTFRQAKIGINHILNVMNNTISCPKSELSTYAPMVQTL
EIQKEKIRDVIGLGGKVIKELCKTFDVEIDISENGEVKVWGNVGENVKKAVQSIENIVFVPQIGDIFDGEVVKVIESGAF
IKYVTGRDGFVHISEINDTHIKDINAHVKLGDKVKVKIIGIDHKNRVKLTLRTDKEHCKNKNEQYNDITTTTGSVKKKIK
IAPKEAAVISNRKYFD
>Mature_736_residues
MFNEILKKVDWHGNMLSLSTGKIARNADGAVLASMGNTSVLCTVVFDKNTKKDIDFFPLGVYYREMAYAAGKIPGGFIKK
EGKFSEYEVLVSRLIDRSIRPLFDSNFRNDTQVICTVMSYDPRYSPDILAIIGSSAALAISGIPIVKPIGAARVGIVNDE
FILNPVIHDNTGVNELDLVVAATFDSVTMIEAQACEIDEEKMLAAIEFGYKSLKPVINAIEEIKSSIRKDIFEVTARPHL
RYNDEILKHFSSDIKSALLLQTKNERNQQLQLIQQKVVDYFSSEANDDDAILNIEKALDDVKSKIFRDLVLQDKTRIGNR
AIDEIRPIICEAGLFNTVHGSALFTRGDTQSLATITLGSSTDEQIVEQLNKCERQNFLLDYIFLPYSVGEISPLRAASRR
EIGHGWLAKKAIQLVIPSKDVFPYTIRIVSEITQSDGSSSMATVCSASLSLMEAGVPIKTHVAGIAMGLVLGEGNKFEIL
SDISGCEDHLGDMDFKVASTKNGITALQLDIKVQGINLSMIESTFRQAKIGINHILNVMNNTISCPKSELSTYAPMVQTL
EIQKEKIRDVIGLGGKVIKELCKTFDVEIDISENGEVKVWGNVGENVKKAVQSIENIVFVPQIGDIFDGEVVKVIESGAF
IKYVTGRDGFVHISEINDTHIKDINAHVKLGDKVKVKIIGIDHKNRVKLTLRTDKEHCKNKNEQYNDITTTTGSVKKKIK
IAPKEAAVISNRKYFD

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=710, Percent_Identity=34.7887323943662, Blast_Score=389, Evalue=1e-108,
Organism=Escherichia coli, GI145693187, Length=702, Percent_Identity=43.3048433048433, Blast_Score=592, Evalue=1e-170,
Organism=Caenorhabditis elegans, GI115534063, Length=718, Percent_Identity=30.9192200557103, Blast_Score=328, Evalue=7e-90,
Organism=Drosophila melanogaster, GI281362905, Length=697, Percent_Identity=32.2812051649928, Blast_Score=374, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24651641, Length=697, Percent_Identity=32.2812051649928, Blast_Score=374, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24651643, Length=697, Percent_Identity=32.2812051649928, Blast_Score=374, Evalue=1e-103,
Organism=Drosophila melanogaster, GI161079377, Length=645, Percent_Identity=32.093023255814, Blast_Score=345, Evalue=6e-95,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 81457; Mature: 81457

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNEILKKVDWHGNMLSLSTGKIARNADGAVLASMGNTSVLCTVVFDKNTKKDIDFFPLG
CHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCEEEHH
VYYREMAYAAGKIPGGFIKKEGKFSEYEVLVSRLIDRSIRPLFDSNFRNDTQVICTVMSY
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEEEEEEC
DPRYSPDILAIIGSSAALAISGIPIVKPIGAARVGIVNDEFILNPVIHDNTGVNELDLVV
CCCCCCCEEEEECCCCEEEECCCCCCCCCCCCEEEEECCCEEECCEEECCCCCCCEEEEE
AATFDSVTMIEAQACEIDEEKMLAAIEFGYKSLKPVINAIEEIKSSIRKDIFEVTARPHL
EEECCCEEEEEHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RYNDEILKHFSSDIKSALLLQTKNERNQQLQLIQQKVVDYFSSEANDDDAILNIEKALDD
CCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHEEEHHHHHHH
VKSKIFRDLVLQDKTRIGNRAIDEIRPIICEAGLFNTVHGSALFTRGDTQSLATITLGSS
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEECCC
TDEQIVEQLNKCERQNFLLDYIFLPYSVGEISPLRAASRREIGHGWLAKKAIQLVIPSKD
CHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHEEEEEECCCC
VFPYTIRIVSEITQSDGSSSMATVCSASLSLMEAGVPIKTHVAGIAMGLVLGEGNKFEIL
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEECCCCCEEEE
SDISGCEDHLGDMDFKVASTKNGITALQLDIKVQGINLSMIESTFRQAKIGINHILNVMN
HHCCCHHHHCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
NTISCPKSELSTYAPMVQTLEIQKEKIRDVIGLGGKVIKELCKTFDVEIDISENGEVKVW
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCEEEE
GNVGENVKKAVQSIENIVFVPQIGDIFDGEVVKVIESGAFIKYVTGRDGFVHISEINDTH
CCCCHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHCCCEEEEEECCCCEEEEEECCCCC
IKDINAHVKLGDKVKVKIIGIDHKNRVKLTLRTDKEHCKNKNEQYNDITTTTGSVKKKIK
EEECCEEEEECCEEEEEEEEECCCCEEEEEEECCHHHHCCCCCCCCCEECCCCCCCEEEE
IAPKEAAVISNRKYFD
ECCCHHHHCCCCCCCC
>Mature Secondary Structure
MFNEILKKVDWHGNMLSLSTGKIARNADGAVLASMGNTSVLCTVVFDKNTKKDIDFFPLG
CHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCEEEHH
VYYREMAYAAGKIPGGFIKKEGKFSEYEVLVSRLIDRSIRPLFDSNFRNDTQVICTVMSY
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEEEEEEC
DPRYSPDILAIIGSSAALAISGIPIVKPIGAARVGIVNDEFILNPVIHDNTGVNELDLVV
CCCCCCCEEEEECCCCEEEECCCCCCCCCCCCEEEEECCCEEECCEEECCCCCCCEEEEE
AATFDSVTMIEAQACEIDEEKMLAAIEFGYKSLKPVINAIEEIKSSIRKDIFEVTARPHL
EEECCCEEEEEHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RYNDEILKHFSSDIKSALLLQTKNERNQQLQLIQQKVVDYFSSEANDDDAILNIEKALDD
CCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHEEEHHHHHHH
VKSKIFRDLVLQDKTRIGNRAIDEIRPIICEAGLFNTVHGSALFTRGDTQSLATITLGSS
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEECCC
TDEQIVEQLNKCERQNFLLDYIFLPYSVGEISPLRAASRREIGHGWLAKKAIQLVIPSKD
CHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHEEEEEECCCC
VFPYTIRIVSEITQSDGSSSMATVCSASLSLMEAGVPIKTHVAGIAMGLVLGEGNKFEIL
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEECCCCCEEEE
SDISGCEDHLGDMDFKVASTKNGITALQLDIKVQGINLSMIESTFRQAKIGINHILNVMN
HHCCCHHHHCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
NTISCPKSELSTYAPMVQTLEIQKEKIRDVIGLGGKVIKELCKTFDVEIDISENGEVKVW
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCEEEE
GNVGENVKKAVQSIENIVFVPQIGDIFDGEVVKVIESGAFIKYVTGRDGFVHISEINDTH
CCCCHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHCCCEEEEEECCCCEEEEEECCCCC
IKDINAHVKLGDKVKVKIIGIDHKNRVKLTLRTDKEHCKNKNEQYNDITTTTGSVKKKIK
EEECCEEEEECCEEEEEEEEECCCCEEEEEEECCHHHHCCCCCCCCCEECCCCCCCEEEE
IAPKEAAVISNRKYFD
ECCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA