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The map label for this gene is proP4 [H]

Identifier: 189184263

GI number: 189184263

Start: 1383968

End: 1385209

Strand: Direct

Name: proP4 [H]

Synonym: OTT_1356

Alternate gene names: 189184263

Gene position: 1383968-1385209 (Clockwise)

Preceding gene: 189184261

Following gene: 189184264

Centisome position: 68.89

GC content: 30.92

Gene sequence:

>1242_bases
ATGTTTCAATCTTATAAAACCTTTTTATTAATCTTGCTTGGTAATGCCCTAGATCATTATAACACAGCTTTATACATATT
TCTAGCTCCATACTTAGCGAGTAATTTTCTTGACTTTAAGAGTGAAGTTATTTCTCTGATTGTAGTACATAGTTTGTTCT
CCTCCTGTACCATTATTGCTAAGCCAGTAGGAGTATGGTTTTTTGGCTGGTTAGTTAATATCGTTGATTCAAGGAAAGTG
TTGTTAATTACTCTAGGTGGAGTAGCTTTTACAACTTTTTCTGTTGCTATTATTCCAAGTTATGAATCTATTGGTATAGC
AGCTACAATTTTGTTAATTCTAGCTAAAGTTGCGCAAGGGTTTTTTGCGGCTGGAGAAGTAGGTATATCATCTATACTTA
TATTTAATACAGTTAAAAGTAAAGAGTTTATTAAAGCTAATAGCTATTACCAGTGCTCAACGATGATAGGTATTATATTG
GCTTCTGCTATTGCTACTATAATAAGTAGCAGCACAGCATCATTTGCGAACTGGCGATATGCTTTTGCTTTAGGAATGCT
AACTGGAATTATTGGATTATGTCTTAGGTTAGTAATATTTTCAAATAGCAATAGTGTCAATTATACTGCATATCACAAAA
ATGTTATGACGCATTCTTCAAAATCTAATCAGACCATATCATCTAAATTTTTCTTATTATTAAAAGTTTCGTGCTTGCAT
GGATTAAGTTATATTACTTATGCTGTTCCATTTGTTATATTAGATAATATTATACCTTTAATTAGCAATATTTCTCGTAC
AGAAATACTTGCATATAGTAATGTACTTATGTATTTTGATGCTGCTATGATAGTAGCTATAGGGCATATAATACGGAGTA
ATAATTATAAAATATGGATGTTATTAGCCGTAATACTATTTGCTGTAACGATAATACCATGCTTTGTTTATCTTTCAAAG
TTACCATTACAAGTCATTATTTTAATCAAATGCTGGATAATATTTTGTGGAGTAGTTTTTACATCATTATTAAATGTGTG
GTTGGTTCAAAAAGTTGATGGTAACAAATATTTATTTATAGGAATTGGGTATGTTATTGGCTGTGAATTCTGGGGCCGCA
GTAGCATCGCAATATGCTTAGCGTTATGGCAATATTTTAATGATCTTATAGCTCCAGCAATTTACATAACAGCAGTATGC
ATTTGTGCAGTTTCATTTCTTTTAATAGATATCTATCGTTAG

Upstream 100 bases:

>100_bases
AATCATTTAATTATTTTTTTAGGTTAGTAGAAAATCAATTAATAAGTATAGATGATATGCAAAAATATAAAATACTTTAT
GCAAATACTTTATAATATTG

Downstream 100 bases:

>100_bases
AAGACATCTTGTTGAGAAGACATCTTGTTGTAAACCTTGAAAATGAAAATTAAACCTGTGCTATATTTTATTAGCTATTT
TCCATAATATTAATTATATA

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MFQSYKTFLLILLGNALDHYNTALYIFLAPYLASNFLDFKSEVISLIVVHSLFSSCTIIAKPVGVWFFGWLVNIVDSRKV
LLITLGGVAFTTFSVAIIPSYESIGIAATILLILAKVAQGFFAAGEVGISSILIFNTVKSKEFIKANSYYQCSTMIGIIL
ASAIATIISSSTASFANWRYAFALGMLTGIIGLCLRLVIFSNSNSVNYTAYHKNVMTHSSKSNQTISSKFFLLLKVSCLH
GLSYITYAVPFVILDNIIPLISNISRTEILAYSNVLMYFDAAMIVAIGHIIRSNNYKIWMLLAVILFAVTIIPCFVYLSK
LPLQVIILIKCWIIFCGVVFTSLLNVWLVQKVDGNKYLFIGIGYVIGCEFWGRSSIAICLALWQYFNDLIAPAIYITAVC
ICAVSFLLIDIYR

Sequences:

>Translated_413_residues
MFQSYKTFLLILLGNALDHYNTALYIFLAPYLASNFLDFKSEVISLIVVHSLFSSCTIIAKPVGVWFFGWLVNIVDSRKV
LLITLGGVAFTTFSVAIIPSYESIGIAATILLILAKVAQGFFAAGEVGISSILIFNTVKSKEFIKANSYYQCSTMIGIIL
ASAIATIISSSTASFANWRYAFALGMLTGIIGLCLRLVIFSNSNSVNYTAYHKNVMTHSSKSNQTISSKFFLLLKVSCLH
GLSYITYAVPFVILDNIIPLISNISRTEILAYSNVLMYFDAAMIVAIGHIIRSNNYKIWMLLAVILFAVTIIPCFVYLSK
LPLQVIILIKCWIIFCGVVFTSLLNVWLVQKVDGNKYLFIGIGYVIGCEFWGRSSIAICLALWQYFNDLIAPAIYITAVC
ICAVSFLLIDIYR
>Mature_413_residues
MFQSYKTFLLILLGNALDHYNTALYIFLAPYLASNFLDFKSEVISLIVVHSLFSSCTIIAKPVGVWFFGWLVNIVDSRKV
LLITLGGVAFTTFSVAIIPSYESIGIAATILLILAKVAQGFFAAGEVGISSILIFNTVKSKEFIKANSYYQCSTMIGIIL
ASAIATIISSSTASFANWRYAFALGMLTGIIGLCLRLVIFSNSNSVNYTAYHKNVMTHSSKSNQTISSKFFLLLKVSCLH
GLSYITYAVPFVILDNIIPLISNISRTEILAYSNVLMYFDAAMIVAIGHIIRSNNYKIWMLLAVILFAVTIIPCFVYLSK
LPLQVIILIKCWIIFCGVVFTSLLNVWLVQKVDGNKYLFIGIGYVIGCEFWGRSSIAICLALWQYFNDLIAPAIYITAVC
ICAVSFLLIDIYR

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=188, Percent_Identity=32.4468085106383, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45799; Mature: 45799

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQSYKTFLLILLGNALDHYNTALYIFLAPYLASNFLDFKSEVISLIVVHSLFSSCTIIA
CCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
KPVGVWFFGWLVNIVDSRKVLLITLGGVAFTTFSVAIIPSYESIGIAATILLILAKVAQG
HHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHH
FFAAGEVGISSILIFNTVKSKEFIKANSYYQCSTMIGIILASAIATIISSSTASFANWRY
HHHHCHHHHHHEEEEEECCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AFALGMLTGIIGLCLRLVIFSNSNSVNYTAYHKNVMTHSSKSNQTISSKFFLLLKVSCLH
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHH
GLSYITYAVPFVILDNIIPLISNISRTEILAYSNVLMYFDAAMIVAIGHIIRSNNYKIWM
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LLAVILFAVTIIPCFVYLSKLPLQVIILIKCWIIFCGVVFTSLLNVWLVQKVDGNKYLFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEE
GIGYVIGCEFWGRSSIAICLALWQYFNDLIAPAIYITAVCICAVSFLLIDIYR
EEHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFQSYKTFLLILLGNALDHYNTALYIFLAPYLASNFLDFKSEVISLIVVHSLFSSCTIIA
CCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
KPVGVWFFGWLVNIVDSRKVLLITLGGVAFTTFSVAIIPSYESIGIAATILLILAKVAQG
HHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHH
FFAAGEVGISSILIFNTVKSKEFIKANSYYQCSTMIGIILASAIATIISSSTASFANWRY
HHHHCHHHHHHEEEEEECCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AFALGMLTGIIGLCLRLVIFSNSNSVNYTAYHKNVMTHSSKSNQTISSKFFLLLKVSCLH
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHH
GLSYITYAVPFVILDNIIPLISNISRTEILAYSNVLMYFDAAMIVAIGHIIRSNNYKIWM
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LLAVILFAVTIIPCFVYLSKLPLQVIILIKCWIIFCGVVFTSLLNVWLVQKVDGNKYLFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEE
GIGYVIGCEFWGRSSIAICLALWQYFNDLIAPAIYITAVCICAVSFLLIDIYR
EEHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]