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The map label for this gene is folD [H]

Identifier: 189184230

GI number: 189184230

Start: 1343275

End: 1344180

Strand: Direct

Name: folD [H]

Synonym: OTT_1323

Alternate gene names: 189184230

Gene position: 1343275-1344180 (Clockwise)

Preceding gene: 189184229

Following gene: 189184235

Centisome position: 66.86

GC content: 30.91

Gene sequence:

>906_bases
ATGACAGTAATTATGGATGGTAAAAAGCTTGCTGAGTTGAGGTTAGTAGAAACAAAAAATGACTTACTAGCTCTTAAAGA
TAAATATCAAATAACAGTTAAGTTGGTAATAATTTTAGTTGGTAACAATGATGCAAGCTTGATTTATGTTAATAATAAGG
TAGCTAAAGCTAAGGCTATTGGTATGGATTCAGAAATAATTAGGTTATTCGAATTCATAGAGGAAAAAAAGTTATTATCT
GTTATTGATGACTTAAATTGTGATAGTACAGTACATGGTATTATTGTTCAATTGCCTCTCCCACCTCATATCGACGCTTT
AAAAATATTTGCAAGAATTGATTCTAGAAAAGATGTTGATGGGCTTAACCCGATAAATATAGGGTATCTTAATATTGGAG
CTAACCATGGATTAATTCCTTGTACAGCTTTAGGATGCATAGATTTATTACAGTATTATGTCACAGATTTGAAGGGTAAA
CATGTAGTTGTAATAGGAAAATCCAATATTGTTGGTAAACCTCTATCAGCATTACTGTTACGTCATTCTTGTACTGTTAC
TATCTGTCATTCTGCTACTGTTGATTTAGCTTTGCATACTAGAACAGCTGATATTGTAATTTCAGCTGTTGGAAAGGCGA
ATTTTTTAACGAATAAGCATTTTTCAGGTAATTTAGCTTTTATTGATGTTGGAATAAGTCATATTTATGATTTGCAAACT
CATAAACGAAAATTAGTTGGAGATGGAGATTTTTTAAAAATCAAAGATTTAGTAAAATTTATTACTCCAGTTCCAGGTGG
GGTAGGGCCAATGACAGTAGCATATCTATTAAAAAATACATTAACTGCTGCTAAACTTATTTATGCGAGCATTATAGATA
ATGATGATGAAAAACACTTGTGTTAG

Upstream 100 bases:

>100_bases
GCTTTGCTGAAGCTGCAACTGCAATGAGTCATTGTTATCAAAGAGTTTTTGGAAAAAAAATGCATTTTCAATACTCTACT
GTAAAGGGAGTATTACGTTC

Downstream 100 bases:

>100_bases
AATAGTTATTATTTTACTATAACATGCTACATTAGTAATTATTTTTACTAAATTTAAGGTCGAATTAATATGATCAAAAT
CTATTAGATTTAACAAACAA

Product: methylenetetrahydrofolate dehydrogenase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTVIMDGKKLAELRLVETKNDLLALKDKYQITVKLVIILVGNNDASLIYVNNKVAKAKAIGMDSEIIRLFEFIEEKKLLS
VIDDLNCDSTVHGIIVQLPLPPHIDALKIFARIDSRKDVDGLNPINIGYLNIGANHGLIPCTALGCIDLLQYYVTDLKGK
HVVVIGKSNIVGKPLSALLLRHSCTVTICHSATVDLALHTRTADIVISAVGKANFLTNKHFSGNLAFIDVGISHIYDLQT
HKRKLVGDGDFLKIKDLVKFITPVPGGVGPMTVAYLLKNTLTAAKLIYASIIDNDDEKHLC

Sequences:

>Translated_301_residues
MTVIMDGKKLAELRLVETKNDLLALKDKYQITVKLVIILVGNNDASLIYVNNKVAKAKAIGMDSEIIRLFEFIEEKKLLS
VIDDLNCDSTVHGIIVQLPLPPHIDALKIFARIDSRKDVDGLNPINIGYLNIGANHGLIPCTALGCIDLLQYYVTDLKGK
HVVVIGKSNIVGKPLSALLLRHSCTVTICHSATVDLALHTRTADIVISAVGKANFLTNKHFSGNLAFIDVGISHIYDLQT
HKRKLVGDGDFLKIKDLVKFITPVPGGVGPMTVAYLLKNTLTAAKLIYASIIDNDDEKHLC
>Mature_300_residues
TVIMDGKKLAELRLVETKNDLLALKDKYQITVKLVIILVGNNDASLIYVNNKVAKAKAIGMDSEIIRLFEFIEEKKLLSV
IDDLNCDSTVHGIIVQLPLPPHIDALKIFARIDSRKDVDGLNPINIGYLNIGANHGLIPCTALGCIDLLQYYVTDLKGKH
VVVIGKSNIVGKPLSALLLRHSCTVTICHSATVDLALHTRTADIVISAVGKANFLTNKHFSGNLAFIDVGISHIYDLQTH
KRKLVGDGDFLKIKDLVKFITPVPGGVGPMTVAYLLKNTLTAAKLIYASIIDNDDEKHLC

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222418558, Length=265, Percent_Identity=47.5471698113208, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI222136639, Length=287, Percent_Identity=39.7212543554007, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI94721354, Length=298, Percent_Identity=41.2751677852349, Blast_Score=183, Evalue=1e-46,
Organism=Escherichia coli, GI1786741, Length=280, Percent_Identity=43.2142857142857, Blast_Score=199, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17568735, Length=269, Percent_Identity=44.2379182156134, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=41.3194444444444, Blast_Score=200, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6321643, Length=293, Percent_Identity=40.9556313993174, Blast_Score=194, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6322933, Length=198, Percent_Identity=28.2828282828283, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17136818, Length=299, Percent_Identity=41.1371237458194, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI17136816, Length=299, Percent_Identity=41.1371237458194, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24645718, Length=285, Percent_Identity=39.6491228070175, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI17137370, Length=285, Percent_Identity=39.6491228070175, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI62472483, Length=285, Percent_Identity=39.6491228070175, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI45551871, Length=285, Percent_Identity=39.6491228070175, Blast_Score=186, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 32908; Mature: 32777

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIMDGKKLAELRLVETKNDLLALKDKYQITVKLVIILVGNNDASLIYVNNKVAKAKAI
CEEEECCCCEEEEEEEECCCCEEEECCCEEEEEEEEEEEECCCCEEEEEEECCEEEHHHC
GMDSEIIRLFEFIEEKKLLSVIDDLNCDSTVHGIIVQLPLPPHIDALKIFARIDSRKDVD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCC
GLNPINIGYLNIGANHGLIPCTALGCIDLLQYYVTDLKGKHVVVIGKSNIVGKPLSALLL
CCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHH
RHSCTVTICHSATVDLALHTRTADIVISAVGKANFLTNKHFSGNLAFIDVGISHIYDLQT
HCCCEEEEEECCEEEEEEEECCCEEEEEECCCCCEEECCCCCCCEEEEEECHHHHHHHHH
HKRKLVGDGDFLKIKDLVKFITPVPGGVGPMTVAYLLKNTLTAAKLIYASIIDNDDEKHL
HHHHHCCCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
C
C
>Mature Secondary Structure 
TVIMDGKKLAELRLVETKNDLLALKDKYQITVKLVIILVGNNDASLIYVNNKVAKAKAI
EEEECCCCEEEEEEEECCCCEEEECCCEEEEEEEEEEEECCCCEEEEEEECCEEEHHHC
GMDSEIIRLFEFIEEKKLLSVIDDLNCDSTVHGIIVQLPLPPHIDALKIFARIDSRKDVD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCC
GLNPINIGYLNIGANHGLIPCTALGCIDLLQYYVTDLKGKHVVVIGKSNIVGKPLSALLL
CCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHH
RHSCTVTICHSATVDLALHTRTADIVISAVGKANFLTNKHFSGNLAFIDVGISHIYDLQT
HCCCEEEEEECCEEEEEEEECCCEEEEEECCCCCEEECCCCCCCEEEEEECHHHHHHHHH
HKRKLVGDGDFLKIKDLVKFITPVPGGVGPMTVAYLLKNTLTAAKLIYASIIDNDDEKHL
HHHHHCCCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
C
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA