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The map label for this gene is ispB [H]

Identifier: 189184004

GI number: 189184004

Start: 1127057

End: 1128073

Strand: Reverse

Name: ispB [H]

Synonym: OTT_1097

Alternate gene names: 189184004

Gene position: 1128073-1127057 (Counterclockwise)

Preceding gene: 189184005

Following gene: 189184003

Centisome position: 56.15

GC content: 28.22

Gene sequence:

>1017_bases
ATGAACTTAAAATTCAGTAACAACTGTAATAGCTTTATCGATAAATTAAGTAAGTTTCTAGATAAAGATATGTCTGAAGT
AAATAATGTTATATTAGACTGCATTTCTTCATCTCAAGAGCAATTAATTTATGAAGTTGGGACATATTTTTTTAATGCAG
GAGGTAAAAGAATACGTCCTATGTTGACTATTATTATCAGTAAGTTATTTAATTACCATGGGCTATCTCACTATTATCTA
GCTGCAGCTGTTGAACTCATTCATTCAGCTACATTGTTACACGATGATGTAGTTGATGAAAGCAAAATGCGCAGATCACA
TCCTACTGTTCATCTTAAATGGGATACTAAAACCAGCATATTAATTGGTGATTTTTTATTTAGTCAGTCGTTTAAGTTAA
TGATTAAAAGCAATTCTATAAGAGCGCTTAAAAGTTTTTCGTTGTCATTTAATACTATTATTACAGGAGAAGTTAAACAG
TTAACACAATTACATAACCAAAAAATCATTACTGAAGATGAGTATTTTAGAGTAATACAAGCTAAAACAGCAGAACTATT
TGGTACTTCATGCGAAATTGCTGCTATAATTTCTAATCAAAGTGCACCAACATGCCTACTAGTTAAAACATTTGGCATTA
AATTAGGGCTGATTTTTCAAATAGTAGATGACATATTAGATTATTTTGGCAACTCAGATAGTTTAGGTAAAAATGTTGGA
GATGATTTTATGGAAGGGAAAGCAACATTACCAATAATTATGGCATATAATTGCGCTTCTTCACAGAATGAAAGAGATTT
TTGGCATAGAATATTCTTGTATCGAGAACAAAAAGAAGATGACTTTCAGTACGCTTTAAGTCTTTTATATACACATAACG
TTTTAGCATTAATAAAACAAAAAATTTCTCAATTGGAACAACAAGTATACGATATTATTACTAAGTTAAATATAAGTTTA
GAATATAAAAATTACTTAGAAAATCTAACTAAATATATTATTAATAGAATAAAATAA

Upstream 100 bases:

>100_bases
AATCGTTCTCCAGAAATTATTACACAAGAATTATGGATGGAAGTTTTAAAATCATTGTTAGAAGCACCAGCATGCCAGTA
GAACTTAAGTATGTAAGTGT

Downstream 100 bases:

>100_bases
TTGTTAGTCTAGTAAGTAGACACACGAGTTACAATTATACGCCAATAATTAGAAACACATCGTGTTTAATTAAGAAAAGA
GCTGCAAAATATAACCATTC

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MNLKFSNNCNSFIDKLSKFLDKDMSEVNNVILDCISSSQEQLIYEVGTYFFNAGGKRIRPMLTIIISKLFNYHGLSHYYL
AAAVELIHSATLLHDDVVDESKMRRSHPTVHLKWDTKTSILIGDFLFSQSFKLMIKSNSIRALKSFSLSFNTIITGEVKQ
LTQLHNQKIITEDEYFRVIQAKTAELFGTSCEIAAIISNQSAPTCLLVKTFGIKLGLIFQIVDDILDYFGNSDSLGKNVG
DDFMEGKATLPIIMAYNCASSQNERDFWHRIFLYREQKEDDFQYALSLLYTHNVLALIKQKISQLEQQVYDIITKLNISL
EYKNYLENLTKYIINRIK

Sequences:

>Translated_338_residues
MNLKFSNNCNSFIDKLSKFLDKDMSEVNNVILDCISSSQEQLIYEVGTYFFNAGGKRIRPMLTIIISKLFNYHGLSHYYL
AAAVELIHSATLLHDDVVDESKMRRSHPTVHLKWDTKTSILIGDFLFSQSFKLMIKSNSIRALKSFSLSFNTIITGEVKQ
LTQLHNQKIITEDEYFRVIQAKTAELFGTSCEIAAIISNQSAPTCLLVKTFGIKLGLIFQIVDDILDYFGNSDSLGKNVG
DDFMEGKATLPIIMAYNCASSQNERDFWHRIFLYREQKEDDFQYALSLLYTHNVLALIKQKISQLEQQVYDIITKLNISL
EYKNYLENLTKYIINRIK
>Mature_338_residues
MNLKFSNNCNSFIDKLSKFLDKDMSEVNNVILDCISSSQEQLIYEVGTYFFNAGGKRIRPMLTIIISKLFNYHGLSHYYL
AAAVELIHSATLLHDDVVDESKMRRSHPTVHLKWDTKTSILIGDFLFSQSFKLMIKSNSIRALKSFSLSFNTIITGEVKQ
LTQLHNQKIITEDEYFRVIQAKTAELFGTSCEIAAIISNQSAPTCLLVKTFGIKLGLIFQIVDDILDYFGNSDSLGKNVG
DDFMEGKATLPIIMAYNCASSQNERDFWHRIFLYREQKEDDFQYALSLLYTHNVLALIKQKISQLEQQVYDIITKLNISL
EYKNYLENLTKYIINRIK

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=232, Percent_Identity=28.448275862069, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI4758430, Length=216, Percent_Identity=25.462962962963, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI83700220, Length=216, Percent_Identity=25.462962962963, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1789578, Length=250, Percent_Identity=40.4, Blast_Score=207, Evalue=6e-55,
Organism=Escherichia coli, GI1786623, Length=314, Percent_Identity=27.3885350318471, Blast_Score=85, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17505681, Length=242, Percent_Identity=30.9917355371901, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319475, Length=309, Percent_Identity=28.8025889967638, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6325188, Length=291, Percent_Identity=26.4604810996564, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24651612, Length=305, Percent_Identity=27.2131147540984, Blast_Score=121, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 38862; Mature: 38862

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKFSNNCNSFIDKLSKFLDKDMSEVNNVILDCISSSQEQLIYEVGTYFFNAGGKRIRP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHH
MLTIIISKLFNYHGLSHYYLAAAVELIHSATLLHDDVVDESKMRRSHPTVHLKWDTKTSI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEECCCCCE
LIGDFLFSQSFKLMIKSNSIRALKSFSLSFNTIITGEVKQLTQLHNQKIITEDEYFRVIQ
EHHHHHHCCCCEEEEECCCHHHHHHHCCEECEEECHHHHHHHHHHCCCCCCCHHHHHHHH
AKTAELFGTSCEIAAIISNQSAPTCLLVKTFGIKLGLIFQIVDDILDYFGNSDSLGKNVG
HHHHHHCCCCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DDFMEGKATLPIIMAYNCASSQNERDFWHRIFLYREQKEDDFQYALSLLYTHNVLALIKQ
HHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KISQLEQQVYDIITKLNISLEYKNYLENLTKYIINRIK
HHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLKFSNNCNSFIDKLSKFLDKDMSEVNNVILDCISSSQEQLIYEVGTYFFNAGGKRIRP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHH
MLTIIISKLFNYHGLSHYYLAAAVELIHSATLLHDDVVDESKMRRSHPTVHLKWDTKTSI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEECCCCCE
LIGDFLFSQSFKLMIKSNSIRALKSFSLSFNTIITGEVKQLTQLHNQKIITEDEYFRVIQ
EHHHHHHCCCCEEEEECCCHHHHHHHCCEECEEECHHHHHHHHHHCCCCCCCHHHHHHHH
AKTAELFGTSCEIAAIISNQSAPTCLLVKTFGIKLGLIFQIVDDILDYFGNSDSLGKNVG
HHHHHHCCCCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DDFMEGKATLPIIMAYNCASSQNERDFWHRIFLYREQKEDDFQYALSLLYTHNVLALIKQ
HHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KISQLEQQVYDIITKLNISLEYKNYLENLTKYIINRIK
HHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]