| Definition | Orientia tsutsugamushi str. Ikeda, complete genome. |
|---|---|
| Accession | NC_010793 |
| Length | 2,008,987 |
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The map label for this gene is tuf [H]
Identifier: 189183949
GI number: 189183949
Start: 1082670
End: 1083854
Strand: Direct
Name: tuf [H]
Synonym: OTT_1042
Alternate gene names: 189183949
Gene position: 1082670-1083854 (Clockwise)
Preceding gene: 189183943
Following gene: 189183950
Centisome position: 53.89
GC content: 37.13
Gene sequence:
>1185_bases ATGGCAGTAGCATTTAATAGAGATAAGCCGCATTGTAATATAGGTACAATAGGGCATGTTGATCATGGAAAAACATCACT AGCAACAGCAATAACTATAGTATCGTCAGAGTTAAGTGATGGTGCTGTAAAGGTGAAGAACTATGATGAAATTGACTCCG CACCAGAAGAAAAAGCTAGAGGGATTACAATACAGACTGCTCATGTAGAGTTTATAAGTAAGAAGAGACATTATGCGCTT GTAGATTGTCCTGGGCATGTTGATTATATTAAAAATATGATCACTGGAGCATCTCAAACTGATGGCTTAATATTAGTTGT ATCTGGTGTTGATGGTGTTATGCCTCAAACAAGAGAGCATGTGTTATTGGCAAAGCAAGTTGGAGTGCCAAGTATTATAG TTTGTATAAATAAGATAGATCAAGCTGATCCTGAATTATTGGAATTAATTGAGATGGAAGTAAGGGAATTGTTGACAAAA TATGATTTTCCAGGTGATACTGTTCCTATAATTAGATGTTCAGCTTTAAAAGCGATAAATGGTGATTCTGATGCTAAGAA AGGTATATTAGAGTTAATGGATGCCATAGATGATTACATACCACAGCCTACTAGAGTTTTAGACCAGCCGTTTTTAATGC CGATAGAGGATGTGTTCTCAATATTAGGTCGCGGTACAGTAGTTACAGGAAGAATTGAGAGAGGGGTTATTAAAGTTGGT GATGAAGTAGAAATAGTTGGATTGCGTAGTACTCAGAAGACAATATGTACTGGTGTTGAGATGTTTAAGAAAGAGTTAGA GCAAGGTCAGGCTGGAGATAATGTAGGTATATTATTACGTGGAATAAAGCGTGAGGATGTAGAAAGGGGGCAGGTTTTAG CTAAGCCTGGTACTATTACTCCGCATTGCAGTTTTGAAGCTGAGGTTTATGTTTTAACAAAAGAGGAAGGTGGTAGGCAT ACTCCGTTTTTTCAAAATTATAGGCCTCAGTTTTATTGTAGAACAACAGATGTTACTGGTGAGATAGCGTTATTATCTGG AAAAGAGATGGTGATGCCTGGGGATCATGCGACATTAAGTATTAATTTAGTAGCGCCTATTGCCATGGATCAAGGGTTAT CTTTTGCTATTCGTGAAGGAGGTAAAACTATTGGTGCTGGCAAAGTTTCTAAAATTATAAAATGA
Upstream 100 bases:
>100_bases GCTCCAAGGCTTGTTAGTTGGTTATTTGTAATATACCACTAGTTGTGTTTATAGAAATATATTTTATTGTAGGTTAAAAG AAGGTTATTATTTAAAGATT
Downstream 100 bases:
>100_bases TGTTCAGAATGGTGATAGAGTTTATTATGTTAAGCGGTAGTTATGAGTAGTAAAGACATTAGAATTAGGTTAAAGTCTTT TGATCATGCTTTATTGGATC
Product: elongation factor Tu
Products: GDP; phosphate
Alternate protein names: EF-Tu 1 [H]
Number of amino acids: Translated: 394; Mature: 393
Protein sequence:
>394_residues MAVAFNRDKPHCNIGTIGHVDHGKTSLATAITIVSSELSDGAVKVKNYDEIDSAPEEKARGITIQTAHVEFISKKRHYAL VDCPGHVDYIKNMITGASQTDGLILVVSGVDGVMPQTREHVLLAKQVGVPSIIVCINKIDQADPELLELIEMEVRELLTK YDFPGDTVPIIRCSALKAINGDSDAKKGILELMDAIDDYIPQPTRVLDQPFLMPIEDVFSILGRGTVVTGRIERGVIKVG DEVEIVGLRSTQKTICTGVEMFKKELEQGQAGDNVGILLRGIKREDVERGQVLAKPGTITPHCSFEAEVYVLTKEEGGRH TPFFQNYRPQFYCRTTDVTGEIALLSGKEMVMPGDHATLSINLVAPIAMDQGLSFAIREGGKTIGAGKVSKIIK
Sequences:
>Translated_394_residues MAVAFNRDKPHCNIGTIGHVDHGKTSLATAITIVSSELSDGAVKVKNYDEIDSAPEEKARGITIQTAHVEFISKKRHYAL VDCPGHVDYIKNMITGASQTDGLILVVSGVDGVMPQTREHVLLAKQVGVPSIIVCINKIDQADPELLELIEMEVRELLTK YDFPGDTVPIIRCSALKAINGDSDAKKGILELMDAIDDYIPQPTRVLDQPFLMPIEDVFSILGRGTVVTGRIERGVIKVG DEVEIVGLRSTQKTICTGVEMFKKELEQGQAGDNVGILLRGIKREDVERGQVLAKPGTITPHCSFEAEVYVLTKEEGGRH TPFFQNYRPQFYCRTTDVTGEIALLSGKEMVMPGDHATLSINLVAPIAMDQGLSFAIREGGKTIGAGKVSKIIK >Mature_393_residues AVAFNRDKPHCNIGTIGHVDHGKTSLATAITIVSSELSDGAVKVKNYDEIDSAPEEKARGITIQTAHVEFISKKRHYALV DCPGHVDYIKNMITGASQTDGLILVVSGVDGVMPQTREHVLLAKQVGVPSIIVCINKIDQADPELLELIEMEVRELLTKY DFPGDTVPIIRCSALKAINGDSDAKKGILELMDAIDDYIPQPTRVLDQPFLMPIEDVFSILGRGTVVTGRIERGVIKVGD EVEIVGLRSTQKTICTGVEMFKKELEQGQAGDNVGILLRGIKREDVERGQVLAKPGTITPHCSFEAEVYVLTKEEGGRHT PFFQNYRPQFYCRTTDVTGEIALLSGKEMVMPGDHATLSINLVAPIAMDQGLSFAIREGGKTIGAGKVSKIIK
Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]
COG id: COG0050
COG function: function code J; GTPases - translation elongation factors
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]
Homologues:
Organism=Homo sapiens, GI34147630, Length=392, Percent_Identity=52.0408163265306, Blast_Score=429, Evalue=1e-120, Organism=Homo sapiens, GI4503471, Length=449, Percent_Identity=29.3986636971047, Blast_Score=161, Evalue=8e-40, Organism=Homo sapiens, GI4503475, Length=446, Percent_Identity=28.47533632287, Blast_Score=159, Evalue=5e-39, Organism=Homo sapiens, GI223555963, Length=425, Percent_Identity=28.4705882352941, Blast_Score=140, Evalue=3e-33, Organism=Homo sapiens, GI5729864, Length=425, Percent_Identity=28.4705882352941, Blast_Score=139, Evalue=4e-33, Organism=Homo sapiens, GI46094014, Length=432, Percent_Identity=28.2407407407407, Blast_Score=128, Evalue=8e-30, Organism=Homo sapiens, GI194018520, Length=433, Percent_Identity=28.4064665127021, Blast_Score=127, Evalue=2e-29, Organism=Homo sapiens, GI194018522, Length=433, Percent_Identity=28.4064665127021, Blast_Score=127, Evalue=2e-29, Organism=Homo sapiens, GI194097354, Length=433, Percent_Identity=28.4064665127021, Blast_Score=126, Evalue=3e-29, Organism=Homo sapiens, GI54607086, Length=325, Percent_Identity=30.1538461538462, Blast_Score=123, Evalue=3e-28, Organism=Homo sapiens, GI157426893, Length=287, Percent_Identity=29.616724738676, Blast_Score=89, Evalue=6e-18, Organism=Homo sapiens, GI82546879, Length=348, Percent_Identity=25.5747126436782, Blast_Score=81, Evalue=2e-15, Organism=Homo sapiens, GI94966754, Length=147, Percent_Identity=36.734693877551, Blast_Score=76, Evalue=5e-14, Organism=Homo sapiens, GI4503507, Length=367, Percent_Identity=27.5204359673025, Blast_Score=72, Evalue=1e-12, Organism=Homo sapiens, GI310132016, Length=120, Percent_Identity=36.6666666666667, Blast_Score=67, Evalue=2e-11, Organism=Homo sapiens, GI310110807, Length=120, Percent_Identity=36.6666666666667, Blast_Score=67, Evalue=2e-11, Organism=Homo sapiens, GI310123363, Length=120, Percent_Identity=36.6666666666667, Blast_Score=67, Evalue=2e-11, Organism=Homo sapiens, GI18390331, Length=133, Percent_Identity=33.8345864661654, Blast_Score=65, Evalue=9e-11, Organism=Escherichia coli, GI1790412, Length=390, Percent_Identity=65.8974358974359, Blast_Score=534, Evalue=1e-153, Organism=Escherichia coli, GI1789737, Length=390, Percent_Identity=65.8974358974359, Blast_Score=534, Evalue=1e-153, Organism=Escherichia coli, GI2367247, Length=337, Percent_Identity=28.486646884273, Blast_Score=130, Evalue=2e-31, Organism=Escherichia coli, GI48994988, Length=276, Percent_Identity=32.2463768115942, Blast_Score=102, Evalue=5e-23, Organism=Escherichia coli, GI1789108, Length=269, Percent_Identity=28.996282527881, Blast_Score=95, Evalue=1e-20, Organism=Escherichia coli, GI1788922, Length=333, Percent_Identity=26.7267267267267, Blast_Score=72, Evalue=5e-14, Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=46.25, Blast_Score=351, Evalue=4e-97, Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=43.0051813471503, Blast_Score=313, Evalue=7e-86, Organism=Caenorhabditis elegans, GI17552884, Length=449, Percent_Identity=28.9532293986637, Blast_Score=159, Evalue=3e-39, Organism=Caenorhabditis elegans, GI17569207, Length=449, Percent_Identity=28.9532293986637, Blast_Score=159, Evalue=3e-39, Organism=Caenorhabditis elegans, GI32566303, Length=442, Percent_Identity=26.4705882352941, Blast_Score=129, Evalue=2e-30, Organism=Caenorhabditis elegans, GI115532067, Length=439, Percent_Identity=28.7015945330296, Blast_Score=124, Evalue=9e-29, Organism=Caenorhabditis elegans, GI32566629, Length=436, Percent_Identity=25.2293577981651, Blast_Score=124, Evalue=1e-28, Organism=Caenorhabditis elegans, GI115532065, Length=439, Percent_Identity=28.7015945330296, Blast_Score=123, Evalue=1e-28, Organism=Caenorhabditis elegans, GI17506081, Length=272, Percent_Identity=30.1470588235294, Blast_Score=108, Evalue=5e-24, Organism=Caenorhabditis elegans, GI17557151, Length=264, Percent_Identity=29.1666666666667, Blast_Score=93, Evalue=2e-19, Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=35.5704697986577, Blast_Score=81, Evalue=8e-16, Organism=Caenorhabditis elegans, GI17509919, Length=444, Percent_Identity=25.4504504504505, Blast_Score=70, Evalue=2e-12, Organism=Caenorhabditis elegans, GI71988811, Length=136, Percent_Identity=30.8823529411765, Blast_Score=69, Evalue=3e-12, Organism=Caenorhabditis elegans, GI71988819, Length=136, Percent_Identity=30.8823529411765, Blast_Score=69, Evalue=3e-12, Organism=Caenorhabditis elegans, GI17556745, Length=129, Percent_Identity=32.5581395348837, Blast_Score=65, Evalue=5e-11, Organism=Saccharomyces cerevisiae, GI6324761, Length=403, Percent_Identity=57.0719602977667, Blast_Score=465, Evalue=1e-132, Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=30.6306306306306, Blast_Score=170, Evalue=3e-43, Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=30.6306306306306, Blast_Score=170, Evalue=3e-43, Organism=Saccharomyces cerevisiae, GI6322937, Length=360, Percent_Identity=26.6666666666667, Blast_Score=92, Evalue=1e-19, Organism=Saccharomyces cerevisiae, GI6320377, Length=329, Percent_Identity=26.1398176291793, Blast_Score=91, Evalue=2e-19, Organism=Saccharomyces cerevisiae, GI6323320, Length=239, Percent_Identity=28.4518828451883, Blast_Score=77, Evalue=6e-15, Organism=Saccharomyces cerevisiae, GI6320863, Length=278, Percent_Identity=26.978417266187, Blast_Score=72, Evalue=1e-13, Organism=Saccharomyces cerevisiae, GI6323098, Length=167, Percent_Identity=30.5389221556886, Blast_Score=65, Evalue=1e-11, Organism=Drosophila melanogaster, GI281363316, Length=392, Percent_Identity=53.3163265306122, Blast_Score=435, Evalue=1e-122, Organism=Drosophila melanogaster, GI17864358, Length=392, Percent_Identity=53.3163265306122, Blast_Score=435, Evalue=1e-122, Organism=Drosophila melanogaster, GI19921738, Length=387, Percent_Identity=47.2868217054264, Blast_Score=370, Evalue=1e-102, Organism=Drosophila melanogaster, GI24652838, Length=448, Percent_Identity=28.3482142857143, Blast_Score=159, Evalue=4e-39, Organism=Drosophila melanogaster, GI17137572, Length=448, Percent_Identity=28.3482142857143, Blast_Score=159, Evalue=4e-39, Organism=Drosophila melanogaster, GI45553807, Length=448, Percent_Identity=27.6785714285714, Blast_Score=154, Evalue=8e-38, Organism=Drosophila melanogaster, GI45553816, Length=448, Percent_Identity=27.6785714285714, Blast_Score=154, Evalue=8e-38, Organism=Drosophila melanogaster, GI24651721, Length=448, Percent_Identity=27.6785714285714, Blast_Score=154, Evalue=8e-38, Organism=Drosophila melanogaster, GI17864154, Length=448, Percent_Identity=27.6785714285714, Blast_Score=154, Evalue=8e-38, Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=30.6930693069307, Blast_Score=129, Evalue=5e-30, Organism=Drosophila melanogaster, GI45550900, Length=432, Percent_Identity=27.5462962962963, Blast_Score=126, Evalue=2e-29, Organism=Drosophila melanogaster, GI17137380, Length=341, Percent_Identity=29.3255131964809, Blast_Score=122, Evalue=6e-28, Organism=Drosophila melanogaster, GI24647048, Length=446, Percent_Identity=26.2331838565022, Blast_Score=77, Evalue=2e-14, Organism=Drosophila melanogaster, GI24647046, Length=446, Percent_Identity=26.2331838565022, Blast_Score=77, Evalue=2e-14, Organism=Drosophila melanogaster, GI28572034, Length=168, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=9e-13, Organism=Drosophila melanogaster, GI78706572, Length=234, Percent_Identity=28.6324786324786, Blast_Score=71, Evalue=2e-12, Organism=Drosophila melanogaster, GI24582462, Length=133, Percent_Identity=33.8345864661654, Blast_Score=69, Evalue=7e-12,
Paralogues:
None
Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000795 - InterPro: IPR005225 - InterPro: IPR009001 - InterPro: IPR004161 - InterPro: IPR004541 - InterPro: IPR004160 - InterPro: IPR009000 [H]
Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]
EC number: 3.6.5.3
Molecular weight: Translated: 42903; Mature: 42772
Theoretical pI: Translated: 5.65; Mature: 5.65
Prosite motif: PS00301 EFACTOR_GTP
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.8 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 4.3 %Cys+Met (Translated Protein) 1.8 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 4.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAVAFNRDKPHCNIGTIGHVDHGKTSLATAITIVSSELSDGAVKVKNYDEIDSAPEEKAR CCEEECCCCCCCCCCCCCCCCCCCHHHHHHHEEEECCCCCCEEEECCCHHCCCCCHHHHC GITIQTAHVEFISKKRHYALVDCPGHVDYIKNMITGASQTDGLILVVSGVDGVMPQTREH CEEEEEEHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHH VLLAKQVGVPSIIVCINKIDQADPELLELIEMEVRELLTKYDFPGDTVPIIRCSALKAIN EEEEHHCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHCCC GDSDAKKGILELMDAIDDYIPQPTRVLDQPFLMPIEDVFSILGRGTVVTGRIERGVIKVG CCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHCCCEEEEEEECCCEEEEC DEVEIVGLRSTQKTICTGVEMFKKELEQGQAGDNVGILLRGIKREDVERGQVLAKPGTIT CEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCEEEECCCCCC PHCSFEAEVYVLTKEEGGRHTPFFQNYRPQFYCRTTDVTGEIALLSGKEMVMPGDHATLS CCCCCCEEEEEEEECCCCCCCCCHHCCCCEEEEEECCCCCEEEEECCCEEECCCCCCEEE INLVAPIAMDQGLSFAIREGGKTIGAGKVSKIIK EEEEEEEECCCCCEEEECCCCCEECCCHHHHHCC >Mature Secondary Structure AVAFNRDKPHCNIGTIGHVDHGKTSLATAITIVSSELSDGAVKVKNYDEIDSAPEEKAR CEEECCCCCCCCCCCCCCCCCCCHHHHHHHEEEECCCCCCEEEECCCHHCCCCCHHHHC GITIQTAHVEFISKKRHYALVDCPGHVDYIKNMITGASQTDGLILVVSGVDGVMPQTREH CEEEEEEHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHH VLLAKQVGVPSIIVCINKIDQADPELLELIEMEVRELLTKYDFPGDTVPIIRCSALKAIN EEEEHHCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHCCC GDSDAKKGILELMDAIDDYIPQPTRVLDQPFLMPIEDVFSILGRGTVVTGRIERGVIKVG CCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHCCCEEEEEEECCCEEEEC DEVEIVGLRSTQKTICTGVEMFKKELEQGQAGDNVGILLRGIKREDVERGQVLAKPGTIT CEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCEEEECCCCCC PHCSFEAEVYVLTKEEGGRHTPFFQNYRPQFYCRTTDVTGEIALLSGKEMVMPGDHATLS CCCCCCEEEEEEEECCCCCCCCCHHCCCCEEEEEECCCCCEEEEECCCEEECCCCCCEEE INLVAPIAMDQGLSFAIREGGKTIGAGKVSKIIK EEEEEEEECCCCCEEEECCCCCEECCCHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: GTP; H2O
Specific reaction: GTP + H2O = GDP + phosphate
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA