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The map label for this gene is gltX [H]

Identifier: 189183880

GI number: 189183880

Start: 1022339

End: 1023787

Strand: Direct

Name: gltX [H]

Synonym: OTT_0973

Alternate gene names: 189183880

Gene position: 1022339-1023787 (Clockwise)

Preceding gene: 189183879

Following gene: 189183882

Centisome position: 50.89

GC content: 31.88

Gene sequence:

>1449_bases
GTGTATCTAACTTATAGTAATATTATGACAAATATAGTAACAAGATTTGCTCCTTCGCCAACTGGTTTTTTACATATTGG
AGGAGCTCGTACTGCTCTTTTTAATTATTTATTTGCTAGACACCATAAAGGCAAGTTTTTGCTTCGTATTGAGGATACTG
ATGCTGCTAGATCAACTGAAGAATATAAACTCGGTATTATAGATAGCTTAAAATGGTTAAAAATAAATTGGGATAATGAT
ATTTTTTATCAGTCTGCAAATCTACAAAGGCATGTGAATGTAGCTTTAGAACTAGTAAAGAGTGGTAAAGCTTACTATTG
TTTTACTTCTCAAGAAGAAATTGATCTCCAAAGACAATTAGCTATTGCACAGAAGCAAAGTTTTATCTTTAGGAGTCCAT
GGCGCAATAACATTCCTTCTTCATTATCATTAAAGAATAATAATAAAGCATATGTTATTCGGTTTAAAGCTCCAAATCAT
GGTACTACTATAATTAATGACTACGTACAAGGTAAGGTAGTATTTCAAAATCAACAGATTGATGATATGATTTTAGTACG
AAGTGATGGTACACCAACTTACATGTTAGCAGTAGTAGTAGATGATCATGATATGGGTATTACACATATTATCCGTGGAG
ATGATCATCTTACGAACGCAGCTAAACAAATAGCATTATATGATGCTTTAGGTTGGAAAGCACCAGCTATGGTTCATATA
CCATTGATTTATGGACCTGATGGAACCAAATTATCTAAAAGGCATGGCGCTATTGGTGTTGATGCATATCAAAAAATGGG
CTATTTGCCTGAAGCTTTATGCAATTATCTATTAAGATTAGGGTGGAGCTACCAAGATGAAGAGATAATATCTCATGAGC
GTGCTATAAAGCTGTTTGACATAGATGGATTAGGTAAGTCTGCAGCTAGATTAGATTTTGATAAAATGTTGTATTTAAAC
GGTTATTATATTCGTAGCACTGATAATTCTATACTAGCAAAACTAGTAATTGAGATACTATCTAAGCAATATATCCTTAG
TAATGAAAGTTGCAATTTGATTCAAAAAGGAATGTCGGGCTTAAAAAGCAGAGCTAATTTACTGACAGATCTTGCTGAAA
ATGCCAAAATATATGTTTTGGAAAATCAGTTAACTTTTGTCAACGAAGCTTTAAATATTATTCAAAAAACTCCTTCTATG
TTAATTACAGAAGTTATTGATATTATTAACAATTTGCAGGAATTAAACTGTGAGTCAGTCAAACAATCTCTTACAGAATT
TGCTAAGGTAAAACATATGAAATTGGGGCAGTTAATGGATCCAATTCGAGCATTATTAACTGGTAATACTAAATCACCTA
GCATATTTGAAGTTATTCCAATATTAGGTAAAATACATGCAGTTAAACGTTTAGCTGATATTGAGGCTATAAAATCAAAC
AATCAATAG

Upstream 100 bases:

>100_bases
TTTATAAAAGATTATTTTTCTCAGATATTTTTTATTATATCTTACATTTTGCAGAATGGATATTATATCGAACTTGTGTT
ACAGATAACATTAAATCTTA

Downstream 100 bases:

>100_bases
TGTGTAAATTATAGTGCAAAAGAGTTACCGCAGCCACATTTAGCTTGGGCTGCTGGATTAGTAATTTTAAAATATGAACG
TCCTAACTCTTCTATATACT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1 [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MYLTYSNIMTNIVTRFAPSPTGFLHIGGARTALFNYLFARHHKGKFLLRIEDTDAARSTEEYKLGIIDSLKWLKINWDND
IFYQSANLQRHVNVALELVKSGKAYYCFTSQEEIDLQRQLAIAQKQSFIFRSPWRNNIPSSLSLKNNNKAYVIRFKAPNH
GTTIINDYVQGKVVFQNQQIDDMILVRSDGTPTYMLAVVVDDHDMGITHIIRGDDHLTNAAKQIALYDALGWKAPAMVHI
PLIYGPDGTKLSKRHGAIGVDAYQKMGYLPEALCNYLLRLGWSYQDEEIISHERAIKLFDIDGLGKSAARLDFDKMLYLN
GYYIRSTDNSILAKLVIEILSKQYILSNESCNLIQKGMSGLKSRANLLTDLAENAKIYVLENQLTFVNEALNIIQKTPSM
LITEVIDIINNLQELNCESVKQSLTEFAKVKHMKLGQLMDPIRALLTGNTKSPSIFEVIPILGKIHAVKRLADIEAIKSN
NQ

Sequences:

>Translated_482_residues
MYLTYSNIMTNIVTRFAPSPTGFLHIGGARTALFNYLFARHHKGKFLLRIEDTDAARSTEEYKLGIIDSLKWLKINWDND
IFYQSANLQRHVNVALELVKSGKAYYCFTSQEEIDLQRQLAIAQKQSFIFRSPWRNNIPSSLSLKNNNKAYVIRFKAPNH
GTTIINDYVQGKVVFQNQQIDDMILVRSDGTPTYMLAVVVDDHDMGITHIIRGDDHLTNAAKQIALYDALGWKAPAMVHI
PLIYGPDGTKLSKRHGAIGVDAYQKMGYLPEALCNYLLRLGWSYQDEEIISHERAIKLFDIDGLGKSAARLDFDKMLYLN
GYYIRSTDNSILAKLVIEILSKQYILSNESCNLIQKGMSGLKSRANLLTDLAENAKIYVLENQLTFVNEALNIIQKTPSM
LITEVIDIINNLQELNCESVKQSLTEFAKVKHMKLGQLMDPIRALLTGNTKSPSIFEVIPILGKIHAVKRLADIEAIKSN
NQ
>Mature_482_residues
MYLTYSNIMTNIVTRFAPSPTGFLHIGGARTALFNYLFARHHKGKFLLRIEDTDAARSTEEYKLGIIDSLKWLKINWDND
IFYQSANLQRHVNVALELVKSGKAYYCFTSQEEIDLQRQLAIAQKQSFIFRSPWRNNIPSSLSLKNNNKAYVIRFKAPNH
GTTIINDYVQGKVVFQNQQIDDMILVRSDGTPTYMLAVVVDDHDMGITHIIRGDDHLTNAAKQIALYDALGWKAPAMVHI
PLIYGPDGTKLSKRHGAIGVDAYQKMGYLPEALCNYLLRLGWSYQDEEIISHERAIKLFDIDGLGKSAARLDFDKMLYLN
GYYIRSTDNSILAKLVIEILSKQYILSNESCNLIQKGMSGLKSRANLLTDLAENAKIYVLENQLTFVNEALNIIQKTPSM
LITEVIDIINNLQELNCESVKQSLTEFAKVKHMKLGQLMDPIRALLTGNTKSPSIFEVIPILGKIHAVKRLADIEAIKSN
NQ

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=500, Percent_Identity=30, Blast_Score=206, Evalue=6e-53,
Organism=Homo sapiens, GI62241042, Length=233, Percent_Identity=25.3218884120172, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI1788743, Length=475, Percent_Identity=36.8421052631579, Blast_Score=331, Evalue=7e-92,
Organism=Escherichia coli, GI226510925, Length=250, Percent_Identity=35.2, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1786895, Length=109, Percent_Identity=33.9449541284404, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71993394, Length=483, Percent_Identity=33.5403726708075, Blast_Score=219, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6324540, Length=489, Percent_Identity=34.1513292433538, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6321192, Length=218, Percent_Identity=28.8990825688073, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324742, Length=108, Percent_Identity=36.1111111111111, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24665321, Length=489, Percent_Identity=32.9243353783231, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24649466, Length=236, Percent_Identity=25.8474576271186, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17864488, Length=133, Percent_Identity=34.5864661654135, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 54563; Mature: 54563

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLTYSNIMTNIVTRFAPSPTGFLHIGGARTALFNYLFARHHKGKFLLRIEDTDAARSTE
CEEEHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCC
EYKLGIIDSLKWLKINWDNDIFYQSANLQRHVNVALELVKSGKAYYCFTSQEEIDLQRQL
CEEEEEECCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
AIAQKQSFIFRSPWRNNIPSSLSLKNNNKAYVIRFKAPNHGTTIINDYVQGKVVFQNQQI
HHHHHHHHEEECCCCCCCCCCEEEECCCEEEEEEEECCCCCCEEEECCCCCEEEEECCCC
DDMILVRSDGTPTYMLAVVVDDHDMGITHIIRGDDHLTNAAKQIALYDALGWKAPAMVHI
CCEEEEECCCCCEEEEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEE
PLIYGPDGTKLSKRHGAIGVDAYQKMGYLPEALCNYLLRLGWSYQDEEIISHERAIKLFD
EEEECCCCCCCHHHCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHCEEEEEE
IDGLGKSAARLDFDKMLYLNGYYIRSTDNSILAKLVIEILSKQYILSNESCNLIQKGMSG
ECCCCCHHHHCCHHHEEEEECEEEEECCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHH
LKSRANLLTDLAENAKIYVLENQLTFVNEALNIIQKTPSMLITEVIDIINNLQELNCESV
HHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHH
KQSLTEFAKVKHMKLGQLMDPIRALLTGNTKSPSIFEVIPILGKIHAVKRLADIEAIKSN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
NQ
CC
>Mature Secondary Structure
MYLTYSNIMTNIVTRFAPSPTGFLHIGGARTALFNYLFARHHKGKFLLRIEDTDAARSTE
CEEEHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCC
EYKLGIIDSLKWLKINWDNDIFYQSANLQRHVNVALELVKSGKAYYCFTSQEEIDLQRQL
CEEEEEECCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
AIAQKQSFIFRSPWRNNIPSSLSLKNNNKAYVIRFKAPNHGTTIINDYVQGKVVFQNQQI
HHHHHHHHEEECCCCCCCCCCEEEECCCEEEEEEEECCCCCCEEEECCCCCEEEEECCCC
DDMILVRSDGTPTYMLAVVVDDHDMGITHIIRGDDHLTNAAKQIALYDALGWKAPAMVHI
CCEEEEECCCCCEEEEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEE
PLIYGPDGTKLSKRHGAIGVDAYQKMGYLPEALCNYLLRLGWSYQDEEIISHERAIKLFD
EEEECCCCCCCHHHCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHCEEEEEE
IDGLGKSAARLDFDKMLYLNGYYIRSTDNSILAKLVIEILSKQYILSNESCNLIQKGMSG
ECCCCCHHHHCCHHHEEEEECEEEEECCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHH
LKSRANLLTDLAENAKIYVLENQLTFVNEALNIIQKTPSMLITEVIDIINNLQELNCESV
HHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHH
KQSLTEFAKVKHMKLGQLMDPIRALLTGNTKSPSIFEVIPILGKIHAVKRLADIEAIKSN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
NQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA