Click here to switch to the map view.

The map label for this gene is alaS [H]

Identifier: 189183879

GI number: 189183879

Start: 1019494

End: 1022163

Strand: Direct

Name: alaS [H]

Synonym: OTT_0972

Alternate gene names: 189183879

Gene position: 1019494-1022163 (Clockwise)

Preceding gene: 189183876

Following gene: 189183880

Centisome position: 50.75

GC content: 31.76

Gene sequence:

>2670_bases
ATGACAACATTTTTAAATATTAATCAAATTCGTACAACCTTTATAGAATTTTTCAAGAAATATGGTCATCATCATGCTAG
TTCGAGTTCTTTAGTTCCAGGTAATGATCCAAGTTTATTTTTTGTGAATGCTGGTATTGTACAATTTAAAGACTATGTTA
GAGCACCTGAAACAAGCAAGTATAGTAGAGTAGTAACTTGTCAAAAATGTGTAAGAGCTGGTGGTAAGCATAATGATTTA
GAAAGTGTTGGCTATACAGCACGCCATCATACTTTTTTTGAAATGCTAGGCAATTTTTCATTTGGGGAAGATAATGCTAA
AGCAGATTTTATGCAGCTTATATGGAATTTTTTAACTAAAGAATTGTTTATTGATGAAGATCGTCTTATTGTTACAGTGT
ACCATACTGATCATGAAACTGCAAAACTATGGCGGTCAATTGCAGGATTAGACGATAGTAGAATTATTCGTATAAAAACA
GATGATAATTTTTGGTCAATGGGGCCTGTTGGTCCATGTGGACCATGTACAGAAATTTTTTATGATCATGGAGACAAGAT
ACCAGGCGGACTGCCTGGAACCAAAGATGAAAATGGTGGACGTTATGTTGAAATATGGAACATAGTTTTCATGCAGTATG
AACAACTTAACGAATCAACTAGAGTTGAACTAGCAAAGCGTTGTATAGATACTGGAGCTGGATTAGAACGGATAGCAACA
GTATTGCAAGGAGTATATGATAATTACGATATTGATTTGTTCAAAAATTTGATTGCAAATATTGAACATTTAACAAAAAT
TAAGTCAGTTGGAGAAGCTAATTTTTCTCATCGGATAATTGCTGATCATTTAAGAGCTAGCGCTTTTTTAATTGCAGATG
GAGTAATGCCTTCTAATGACGGCAGAGGATATGTTTTAAGAAGAATAATGAGAAGAGCTATGAATCAGATTCATCAACTG
GGCTGTAAAGAACCTGTTATGCATCTGCTAGTTCAAGGTTTAATTAATGAAATGGGTGATTTTTATAAGGAATTAAGAGT
TAGGCAAGAACTCATTACTTACTTGTTAAAAAATGAAGAGGAAAAATTTAAGACTACTTTATCCAAGGGATTAAAGTTAC
TTGAAGAAGAAAGTAAGAATCTAACATCTGGAAGTCAGCTTTCTGGGCACATTGCATTCAAGCTTTATGATACATATGGG
TTTCCTTTTGACTTAACTCAAGATATTCTAAAAAAGAGAAGCATTTCAATTAATAAAACAGAATTTGACCAAAACATGCT
TGAACAACAAAATCGGGCAAGGCAATTGTGGAAAGGGCAGGGTAGTAATAAAGAGCAGCTATTATGGGAAAAATTAAAAG
AAGAATTTAGAGCTACAGAATTTGTAGGATATAGTCTTTGTCAAGCCGAAGGAATTGTAGTATCTCTCATTCAAGATAAT
CAATATGTAGAATATATTGATATAAATCAAGTTGATGACAACAATAAAGAATTTTGGCTTATAACTAATCAGACTCCATT
TTATGGTCAGTCTGGCGGACAAATTGGAGATATAGGAATAATTAAAAATAATGAATGTACAATTCATGTAACTGATACTA
TAAAGCTTTTTGGTTGTACTCATGTGCATATTTGTAAAATAGTATCAGGTAAAATTAATCTTAATGCTGTAGTACATATG
GCAATTGACAAGCAATATCGTACTCAATTGCAAATTCATCATTCAGCAACACATATTTTGCATGCAGCTTTAAGAGAAAT
TTTAGGTAATCATATCATTCAAAAAGGATCATTAGTAGCTTATGACTATCTACGTTTTGATGTTTCGCATCCAACATCTA
TTAGTAAAGATCTTTTAACTAAAATTGAAAATAGAGTTAATGAAATAATTCTTAACAATACTGCGGTTAAACTTAGGATA
ATGCCATTTGATCAAGCGATAGCTCATGGAGCTGTTGCTTTGTTTGAAGAAAAATATGGGGATGAAGTTAGGGCAATATC
TATAGGAGAAACATCTGATGCTAGATATTACTCCTTTGAATTATGTGGTGGAACTCATGTGAAATACACTGGAGATATTG
GAGCATTTAGAATTTTGTCAGAAAGCGCGATTGCTGCTGGAGTTAGAAGAATAGAGGCAATTGCTGGCAAGCATGTCATT
AAACAAGCTAGGCAGAATAGCGAACTACTTGATCTAATTGCAGAAAAATTTAGCGTTACTAAGCAAACCATAATGTCTAA
AATTGATGGCATTATAGAAGAGAATAATTTATTAAAAAAACAGTTGCATCAATTAAAATATAATCAACTTATTTTGTGTG
AAAAAGATATACAAAATATGGCTGATAATATTGGAAAAATAAAATTAGTTTACAAAAATATTGAAGATTATGATCTTCAA
ATTGTTAGAAAAGCTGTAAGTAATACAACGAAAAATATTCAGAACTTAGTTATGGTTGTTATAAGTAATAATGATAAAAA
AAGCACAATTATAATTGGAGTGAGTGATAATATTACCAATAAGATACAGGCTAATAACTTAGTAAAGGAAATTATAAATT
ATATAGGTGGTAGTGGAGGAGGAAGCGCTACTCTTGCTCAAATAGGTTGCCAGTATACCTCCAAACTTCTAGATTTAAAG
AATATAATTTGTAAGTTGTTAGCTACATAG

Upstream 100 bases:

>100_bases
TTAGAACAGTATATATTGTACATTATACTATGTTTTTCGAACTTATAGCAACTAGCCTAAAAAATAATAACAAGGCGCGT
GTATCATCTTAAAATTGCAA

Downstream 100 bases:

>100_bases
CCTAACAATAGAGTACAATTAGTATATTATAACACGAGTATGACATAAGTTTGATATATTGATTCTAACTCTTATTTTAT
AAAAGATTATTTTTCTCAGA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 889; Mature: 888

Protein sequence:

>889_residues
MTTFLNINQIRTTFIEFFKKYGHHHASSSSLVPGNDPSLFFVNAGIVQFKDYVRAPETSKYSRVVTCQKCVRAGGKHNDL
ESVGYTARHHTFFEMLGNFSFGEDNAKADFMQLIWNFLTKELFIDEDRLIVTVYHTDHETAKLWRSIAGLDDSRIIRIKT
DDNFWSMGPVGPCGPCTEIFYDHGDKIPGGLPGTKDENGGRYVEIWNIVFMQYEQLNESTRVELAKRCIDTGAGLERIAT
VLQGVYDNYDIDLFKNLIANIEHLTKIKSVGEANFSHRIIADHLRASAFLIADGVMPSNDGRGYVLRRIMRRAMNQIHQL
GCKEPVMHLLVQGLINEMGDFYKELRVRQELITYLLKNEEEKFKTTLSKGLKLLEEESKNLTSGSQLSGHIAFKLYDTYG
FPFDLTQDILKKRSISINKTEFDQNMLEQQNRARQLWKGQGSNKEQLLWEKLKEEFRATEFVGYSLCQAEGIVVSLIQDN
QYVEYIDINQVDDNNKEFWLITNQTPFYGQSGGQIGDIGIIKNNECTIHVTDTIKLFGCTHVHICKIVSGKINLNAVVHM
AIDKQYRTQLQIHHSATHILHAALREILGNHIIQKGSLVAYDYLRFDVSHPTSISKDLLTKIENRVNEIILNNTAVKLRI
MPFDQAIAHGAVALFEEKYGDEVRAISIGETSDARYYSFELCGGTHVKYTGDIGAFRILSESAIAAGVRRIEAIAGKHVI
KQARQNSELLDLIAEKFSVTKQTIMSKIDGIIEENNLLKKQLHQLKYNQLILCEKDIQNMADNIGKIKLVYKNIEDYDLQ
IVRKAVSNTTKNIQNLVMVVISNNDKKSTIIIGVSDNITNKIQANNLVKEIINYIGGSGGGSATLAQIGCQYTSKLLDLK
NIICKLLAT

Sequences:

>Translated_889_residues
MTTFLNINQIRTTFIEFFKKYGHHHASSSSLVPGNDPSLFFVNAGIVQFKDYVRAPETSKYSRVVTCQKCVRAGGKHNDL
ESVGYTARHHTFFEMLGNFSFGEDNAKADFMQLIWNFLTKELFIDEDRLIVTVYHTDHETAKLWRSIAGLDDSRIIRIKT
DDNFWSMGPVGPCGPCTEIFYDHGDKIPGGLPGTKDENGGRYVEIWNIVFMQYEQLNESTRVELAKRCIDTGAGLERIAT
VLQGVYDNYDIDLFKNLIANIEHLTKIKSVGEANFSHRIIADHLRASAFLIADGVMPSNDGRGYVLRRIMRRAMNQIHQL
GCKEPVMHLLVQGLINEMGDFYKELRVRQELITYLLKNEEEKFKTTLSKGLKLLEEESKNLTSGSQLSGHIAFKLYDTYG
FPFDLTQDILKKRSISINKTEFDQNMLEQQNRARQLWKGQGSNKEQLLWEKLKEEFRATEFVGYSLCQAEGIVVSLIQDN
QYVEYIDINQVDDNNKEFWLITNQTPFYGQSGGQIGDIGIIKNNECTIHVTDTIKLFGCTHVHICKIVSGKINLNAVVHM
AIDKQYRTQLQIHHSATHILHAALREILGNHIIQKGSLVAYDYLRFDVSHPTSISKDLLTKIENRVNEIILNNTAVKLRI
MPFDQAIAHGAVALFEEKYGDEVRAISIGETSDARYYSFELCGGTHVKYTGDIGAFRILSESAIAAGVRRIEAIAGKHVI
KQARQNSELLDLIAEKFSVTKQTIMSKIDGIIEENNLLKKQLHQLKYNQLILCEKDIQNMADNIGKIKLVYKNIEDYDLQ
IVRKAVSNTTKNIQNLVMVVISNNDKKSTIIIGVSDNITNKIQANNLVKEIINYIGGSGGGSATLAQIGCQYTSKLLDLK
NIICKLLAT
>Mature_888_residues
TTFLNINQIRTTFIEFFKKYGHHHASSSSLVPGNDPSLFFVNAGIVQFKDYVRAPETSKYSRVVTCQKCVRAGGKHNDLE
SVGYTARHHTFFEMLGNFSFGEDNAKADFMQLIWNFLTKELFIDEDRLIVTVYHTDHETAKLWRSIAGLDDSRIIRIKTD
DNFWSMGPVGPCGPCTEIFYDHGDKIPGGLPGTKDENGGRYVEIWNIVFMQYEQLNESTRVELAKRCIDTGAGLERIATV
LQGVYDNYDIDLFKNLIANIEHLTKIKSVGEANFSHRIIADHLRASAFLIADGVMPSNDGRGYVLRRIMRRAMNQIHQLG
CKEPVMHLLVQGLINEMGDFYKELRVRQELITYLLKNEEEKFKTTLSKGLKLLEEESKNLTSGSQLSGHIAFKLYDTYGF
PFDLTQDILKKRSISINKTEFDQNMLEQQNRARQLWKGQGSNKEQLLWEKLKEEFRATEFVGYSLCQAEGIVVSLIQDNQ
YVEYIDINQVDDNNKEFWLITNQTPFYGQSGGQIGDIGIIKNNECTIHVTDTIKLFGCTHVHICKIVSGKINLNAVVHMA
IDKQYRTQLQIHHSATHILHAALREILGNHIIQKGSLVAYDYLRFDVSHPTSISKDLLTKIENRVNEIILNNTAVKLRIM
PFDQAIAHGAVALFEEKYGDEVRAISIGETSDARYYSFELCGGTHVKYTGDIGAFRILSESAIAAGVRRIEAIAGKHVIK
QARQNSELLDLIAEKFSVTKQTIMSKIDGIIEENNLLKKQLHQLKYNQLILCEKDIQNMADNIGKIKLVYKNIEDYDLQI
VRKAVSNTTKNIQNLVMVVISNNDKKSTIIIGVSDNITNKIQANNLVKEIINYIGGSGGGSATLAQIGCQYTSKLLDLKN
IICKLLAT

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=972, Percent_Identity=32.201646090535, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI38569417, Length=946, Percent_Identity=27.906976744186, Blast_Score=368, Evalue=1e-101,
Organism=Escherichia coli, GI1789048, Length=870, Percent_Identity=44.1379310344828, Blast_Score=733, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=968, Percent_Identity=30.6818181818182, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17536681, Length=741, Percent_Identity=31.9838056680162, Blast_Score=332, Evalue=7e-91,
Organism=Saccharomyces cerevisiae, GI6324911, Length=776, Percent_Identity=35.5670103092784, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24582809, Length=786, Percent_Identity=34.3511450381679, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI45552267, Length=786, Percent_Identity=34.3511450381679, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24658214, Length=838, Percent_Identity=29.1169451073986, Blast_Score=307, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 100620; Mature: 100488

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFLNINQIRTTFIEFFKKYGHHHASSSSLVPGNDPSLFFVNAGIVQFKDYVRAPETSK
CCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCC
YSRVVTCQKCVRAGGKHNDLESVGYTARHHTFFEMLGNFSFGEDNAKADFMQLIWNFLTK
HHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
ELFIDEDRLIVTVYHTDHETAKLWRSIAGLDDSRIIRIKTDDNFWSMGPVGPCGPCTEIF
HHEECCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCCCCCHHHHH
YDHGDKIPGGLPGTKDENGGRYVEIWNIVFMQYEQLNESTRVELAKRCIDTGAGLERIAT
HCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHH
VLQGVYDNYDIDLFKNLIANIEHLTKIKSVGEANFSHRIIADHLRASAFLIADGVMPSND
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCEEEEECCCCCCCC
GRGYVLRRIMRRAMNQIHQLGCKEPVMHLLVQGLINEMGDFYKELRVRQELITYLLKNEE
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EKFKTTLSKGLKLLEEESKNLTSGSQLSGHIAFKLYDTYGFPFDLTQDILKKRSISINKT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCCHH
EFDQNMLEQQNRARQLWKGQGSNKEQLLWEKLKEEFRATEFVGYSLCQAEGIVVSLIQDN
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
QYVEYIDINQVDDNNKEFWLITNQTPFYGQSGGQIGDIGIIKNNECTIHVTDTIKLFGCT
CEEEEEECEECCCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCEEEEEECEEEEECCC
HVHICKIVSGKINLNAVVHMAIDKQYRTQLQIHHSATHILHAALREILGNHIIQKGSLVA
CEEEEEEECCCEEEEEEEEEECCHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YDYLRFDVSHPTSISKDLLTKIENRVNEIILNNTAVKLRIMPFDQAIAHGAVALFEEKYG
EEEEEEECCCCCCHHHHHHHHHHHHHHHEEECCCEEEEEEECHHHHHHHHHHHHHHHHCC
DEVRAISIGETSDARYYSFELCGGTHVKYTGDIGAFRILSESAIAAGVRRIEAIAGKHVI
CCEEEEEECCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KQARQNSELLDLIAEKFSVTKQTIMSKIDGIIEENNLLKKQLHQLKYNQLILCEKDIQNM
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEHHHHHHH
ADNIGKIKLVYKNIEDYDLQIVRKAVSNTTKNIQNLVMVVISNNDKKSTIIIGVSDNITN
HHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEEECCCCCC
KIQANNLVKEIINYIGGSGGGSATLAQIGCQYTSKLLDLKNIICKLLAT
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTFLNINQIRTTFIEFFKKYGHHHASSSSLVPGNDPSLFFVNAGIVQFKDYVRAPETSK
CEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCC
YSRVVTCQKCVRAGGKHNDLESVGYTARHHTFFEMLGNFSFGEDNAKADFMQLIWNFLTK
HHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
ELFIDEDRLIVTVYHTDHETAKLWRSIAGLDDSRIIRIKTDDNFWSMGPVGPCGPCTEIF
HHEECCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCCCCCHHHHH
YDHGDKIPGGLPGTKDENGGRYVEIWNIVFMQYEQLNESTRVELAKRCIDTGAGLERIAT
HCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHH
VLQGVYDNYDIDLFKNLIANIEHLTKIKSVGEANFSHRIIADHLRASAFLIADGVMPSND
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCEEEEECCCCCCCC
GRGYVLRRIMRRAMNQIHQLGCKEPVMHLLVQGLINEMGDFYKELRVRQELITYLLKNEE
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EKFKTTLSKGLKLLEEESKNLTSGSQLSGHIAFKLYDTYGFPFDLTQDILKKRSISINKT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCCHH
EFDQNMLEQQNRARQLWKGQGSNKEQLLWEKLKEEFRATEFVGYSLCQAEGIVVSLIQDN
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
QYVEYIDINQVDDNNKEFWLITNQTPFYGQSGGQIGDIGIIKNNECTIHVTDTIKLFGCT
CEEEEEECEECCCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCEEEEEECEEEEECCC
HVHICKIVSGKINLNAVVHMAIDKQYRTQLQIHHSATHILHAALREILGNHIIQKGSLVA
CEEEEEEECCCEEEEEEEEEECCHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YDYLRFDVSHPTSISKDLLTKIENRVNEIILNNTAVKLRIMPFDQAIAHGAVALFEEKYG
EEEEEEECCCCCCHHHHHHHHHHHHHHHEEECCCEEEEEEECHHHHHHHHHHHHHHHHCC
DEVRAISIGETSDARYYSFELCGGTHVKYTGDIGAFRILSESAIAAGVRRIEAIAGKHVI
CCEEEEEECCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KQARQNSELLDLIAEKFSVTKQTIMSKIDGIIEENNLLKKQLHQLKYNQLILCEKDIQNM
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEHHHHHHH
ADNIGKIKLVYKNIEDYDLQIVRKAVSNTTKNIQNLVMVVISNNDKKSTIIIGVSDNITN
HHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEEECCCCCC
KIQANNLVKEIINYIGGSGGGSATLAQIGCQYTSKLLDLKNIICKLLAT
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA