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The map label for this gene is glyS [H]

Identifier: 189183850

GI number: 189183850

Start: 985050

End: 987101

Strand: Reverse

Name: glyS [H]

Synonym: OTT_0943

Alternate gene names: 189183850

Gene position: 987101-985050 (Counterclockwise)

Preceding gene: 189183851

Following gene: 189183849

Centisome position: 49.13

GC content: 30.26

Gene sequence:

>2052_bases
ATGCATAGTTACAGTTTAATATTAGAACTCTACTCTGAGGAAATTCCAGCTAAAATGCAAAAAAAAGCTGAATTAGCATT
TCAAGAAATTTTTACTAAAAATTTCAAATTAGCTAATATTAGTTTTAATCACTTAAAAGTATATATAGGACCATGCAGAG
TGGTTCTACATACAGTCTTTAGCAGCAAAGAACTAGCCGCTAAAGCAATTGAACATAAAGGCCCTAAAGTCGGAGCAAAA
AAATCAGCTGTTGAAGGATTTTGCAAAGCCAAGTCTATTCTAAGCAAGGATTTAACCATAAAAACATTTAATAATCAGCA
GTTCTACGTTTATACTCATGTTACTCCAACTCAACCTATTAAAGATCTATTACCAGAAATCATATTAAACTCCGTTAAAG
AATACACTTGGCCAAAATCAATGAAATGGGAAAATTATCAGATTAATTGGGTTAGACCACTTAGAAACATTTTATGCATG
TTCAATGGCGAAGAACTAAAAATCAGCTGGCATCATCTAACATCAAATAATAAAAGCTTTGGACATAAATTCATGAGTCC
AAATAGCTTTACTGTCAGCACCTGGCAAGATTATACTAATAATTTAAGAGATAATTTTGTCATATTAGATAGATCAGAAC
GGCAAGCTATTATACTTAATTCCTTAGAAAAAACATGCAAAACTCTAGGTATCACCTTAAATTACAATAATAAATTACTT
GATGAAGTTACTGGTTTAGTTGAATACCCTATCGTATTATATGGCACAATACCTAATGAGTTTCTTAGCTTACCAGAAGA
AATCATCATCACTTCTGTACAAACTCATCAAAAATATTTCACTACAAAAGATCATAACAATAAACTAGCTCCATATTTTT
TATTTGTTACCAATATCAATACTGAAGATTATAGCAATATTATCAAAGGAAATGAAAAAGTCTTATCATCTCGTCTCTCT
GATGCTATATACTTTTTTAATCAAGATTGTCAATTTACATTGCTTAGTAAATTAGAAAAACTAAAAAAAGTAGTCTTTCA
CACTAAACTTGGCAGTATATTTGATAAAGTTCAGAGAATAAAAAAAATATGCCATAAGCTTGCACCTGATGATCAAGAAC
TAGCTATTGCAGCTGAACTGTGTAAATCAGATTTAGTCTCAGAAGTTGTACAAGAATTCCCTAATCTACAAGGAATAATG
GGAAAATACTATGCTTTAAATGACAAACTCAGCCCTGCAGTCGCTAATATAATCGGCAACCACTACCATCCTACTGGAAT
TGAAGATGAAGTACCTAATGGCAAGGCAGCAATTCTTGCTTTAGCTGACAAGCTTGATAATTTAACTAGCCTATATTTAG
CAGGAGAGCGCGCTACTAGCTCAAAAGATCCATTTGCATTACGTAGATGCGCTTTTTCTATCGCTAGAATAATTTTAACT
AATAAAATACAAGTAAAATTATTAGATTTATTAACTTTCATTTATCAACTTCATGATATTTATACTAATGAGCTAGCAAC
CGAAGTACTACTATTCATAAAAGATAAAACAAAAAATTTACTTAATGCAAAATTTAATCATTTGTTAGTCGAAGCTATAT
TTCAGCAAAATAACTATGATGATCTTACTGAGTTAGAATTAAGAATGAATATTCTTGATCATTTTTTAACCTCCACTAAA
GGTCAAAACTTTATACAATCATATAAAAGAGCGTATAATATCATTAAAGATGAAGAAATTCGCCAAGCAAAAATAGATGT
TTGCAAATTATTAACTAAACAAGAAACCATCTTATATCAGCAAGCTGTGGATGCAAAAATCAGCCAATTATTTACTCAAT
TATCAGCCGAAACTTTTTCTTTACTTCTTGATCAATTATCAAATATTGGCATATCAATTGCAAAATTTTTAGATAATGTC
CCTGTTAACACTACTGATCAGTGTCTTACTTATAATCGCAAGCTTATATTACAACATGTAGTACAGTGGTGCAATAATAT
AGCACCATTTGACAAAGTAATAGAACATTATGTTAACAAATATGAACAGTAA

Upstream 100 bases:

>100_bases
GGAGTAATCGGAGTTACTCAACGCACTAATTATATCGCTAGAATACGAACACTAGCTCAAACAGTATGTAATCAATATAT
AAAAAATCTTAATATATCAA

Downstream 100 bases:

>100_bases
TAATAATGATATAAATGTTGCTGCACAATTTATTGAACAAGGGAAATTAGTCATATTCCCTACTGAAACAGTGTATGGCA
TAGGGGCTGACGCAACTAAC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 683; Mature: 683

Protein sequence:

>683_residues
MHSYSLILELYSEEIPAKMQKKAELAFQEIFTKNFKLANISFNHLKVYIGPCRVVLHTVFSSKELAAKAIEHKGPKVGAK
KSAVEGFCKAKSILSKDLTIKTFNNQQFYVYTHVTPTQPIKDLLPEIILNSVKEYTWPKSMKWENYQINWVRPLRNILCM
FNGEELKISWHHLTSNNKSFGHKFMSPNSFTVSTWQDYTNNLRDNFVILDRSERQAIILNSLEKTCKTLGITLNYNNKLL
DEVTGLVEYPIVLYGTIPNEFLSLPEEIIITSVQTHQKYFTTKDHNNKLAPYFLFVTNINTEDYSNIIKGNEKVLSSRLS
DAIYFFNQDCQFTLLSKLEKLKKVVFHTKLGSIFDKVQRIKKICHKLAPDDQELAIAAELCKSDLVSEVVQEFPNLQGIM
GKYYALNDKLSPAVANIIGNHYHPTGIEDEVPNGKAAILALADKLDNLTSLYLAGERATSSKDPFALRRCAFSIARIILT
NKIQVKLLDLLTFIYQLHDIYTNELATEVLLFIKDKTKNLLNAKFNHLLVEAIFQQNNYDDLTELELRMNILDHFLTSTK
GQNFIQSYKRAYNIIKDEEIRQAKIDVCKLLTKQETILYQQAVDAKISQLFTQLSAETFSLLLDQLSNIGISIAKFLDNV
PVNTTDQCLTYNRKLILQHVVQWCNNIAPFDKVIEHYVNKYEQ

Sequences:

>Translated_683_residues
MHSYSLILELYSEEIPAKMQKKAELAFQEIFTKNFKLANISFNHLKVYIGPCRVVLHTVFSSKELAAKAIEHKGPKVGAK
KSAVEGFCKAKSILSKDLTIKTFNNQQFYVYTHVTPTQPIKDLLPEIILNSVKEYTWPKSMKWENYQINWVRPLRNILCM
FNGEELKISWHHLTSNNKSFGHKFMSPNSFTVSTWQDYTNNLRDNFVILDRSERQAIILNSLEKTCKTLGITLNYNNKLL
DEVTGLVEYPIVLYGTIPNEFLSLPEEIIITSVQTHQKYFTTKDHNNKLAPYFLFVTNINTEDYSNIIKGNEKVLSSRLS
DAIYFFNQDCQFTLLSKLEKLKKVVFHTKLGSIFDKVQRIKKICHKLAPDDQELAIAAELCKSDLVSEVVQEFPNLQGIM
GKYYALNDKLSPAVANIIGNHYHPTGIEDEVPNGKAAILALADKLDNLTSLYLAGERATSSKDPFALRRCAFSIARIILT
NKIQVKLLDLLTFIYQLHDIYTNELATEVLLFIKDKTKNLLNAKFNHLLVEAIFQQNNYDDLTELELRMNILDHFLTSTK
GQNFIQSYKRAYNIIKDEEIRQAKIDVCKLLTKQETILYQQAVDAKISQLFTQLSAETFSLLLDQLSNIGISIAKFLDNV
PVNTTDQCLTYNRKLILQHVVQWCNNIAPFDKVIEHYVNKYEQ
>Mature_683_residues
MHSYSLILELYSEEIPAKMQKKAELAFQEIFTKNFKLANISFNHLKVYIGPCRVVLHTVFSSKELAAKAIEHKGPKVGAK
KSAVEGFCKAKSILSKDLTIKTFNNQQFYVYTHVTPTQPIKDLLPEIILNSVKEYTWPKSMKWENYQINWVRPLRNILCM
FNGEELKISWHHLTSNNKSFGHKFMSPNSFTVSTWQDYTNNLRDNFVILDRSERQAIILNSLEKTCKTLGITLNYNNKLL
DEVTGLVEYPIVLYGTIPNEFLSLPEEIIITSVQTHQKYFTTKDHNNKLAPYFLFVTNINTEDYSNIIKGNEKVLSSRLS
DAIYFFNQDCQFTLLSKLEKLKKVVFHTKLGSIFDKVQRIKKICHKLAPDDQELAIAAELCKSDLVSEVVQEFPNLQGIM
GKYYALNDKLSPAVANIIGNHYHPTGIEDEVPNGKAAILALADKLDNLTSLYLAGERATSSKDPFALRRCAFSIARIILT
NKIQVKLLDLLTFIYQLHDIYTNELATEVLLFIKDKTKNLLNAKFNHLLVEAIFQQNNYDDLTELELRMNILDHFLTSTK
GQNFIQSYKRAYNIIKDEEIRQAKIDVCKLLTKQETILYQQAVDAKISQLFTQLSAETFSLLLDQLSNIGISIAKFLDNV
PVNTTDQCLTYNRKLILQHVVQWCNNIAPFDKVIEHYVNKYEQ

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=28.1567489114659, Blast_Score=285, Evalue=6e-78,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 78550; Mature: 78550

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSYSLILELYSEEIPAKMQKKAELAFQEIFTKNFKLANISFNHLKVYIGPCRVVLHTVF
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEECEEEEEEHHHHHHHHHHH
SSKELAAKAIEHKGPKVGAKKSAVEGFCKAKSILSKDLTIKTFNNQQFYVYTHVTPTQPI
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEECCCCCHH
KDLLPEIILNSVKEYTWPKSMKWENYQINWVRPLRNILCMFNGEELKISWHHLTSNNKSF
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHCCCCEEEEEEEEECCCCCHH
GHKFMSPNSFTVSTWQDYTNNLRDNFVILDRSERQAIILNSLEKTCKTLGITLNYNNKLL
CCCCCCCCCEEEECHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHEEEEEECCCHHH
DEVTGLVEYPIVLYGTIPNEFLSLPEEIIITSVQTHQKYFTTKDHNNKLAPYFLFVTNIN
HHHHHHHHCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECC
TEDYSNIIKGNEKVLSSRLSDAIYFFNQDCQFTLLSKLEKLKKVVFHTKLGSIFDKVQRI
CHHHHHHHCCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKICHKLAPDDQELAIAAELCKSDLVSEVVQEFPNLQGIMGKYYALNDKLSPAVANIIGN
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCCHHHHHHHCC
HYHPTGIEDEVPNGKAAILALADKLDNLTSLYLAGERATSSKDPFALRRCAFSIARIILT
CCCCCCCCCCCCCCCEEHHHHHHHHCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHH
NKIQVKLLDLLTFIYQLHDIYTNELATEVLLFIKDKTKNLLNAKFNHLLVEAIFQQNNYD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DLTELELRMNILDHFLTSTKGQNFIQSYKRAYNIIKDEEIRQAKIDVCKLLTKQETILYQ
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
QAVDAKISQLFTQLSAETFSLLLDQLSNIGISIAKFLDNVPVNTTDQCLTYNRKLILQHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VQWCNNIAPFDKVIEHYVNKYEQ
HHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MHSYSLILELYSEEIPAKMQKKAELAFQEIFTKNFKLANISFNHLKVYIGPCRVVLHTVF
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEECEEEEEEHHHHHHHHHHH
SSKELAAKAIEHKGPKVGAKKSAVEGFCKAKSILSKDLTIKTFNNQQFYVYTHVTPTQPI
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEECCCCCHH
KDLLPEIILNSVKEYTWPKSMKWENYQINWVRPLRNILCMFNGEELKISWHHLTSNNKSF
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHCCCCEEEEEEEEECCCCCHH
GHKFMSPNSFTVSTWQDYTNNLRDNFVILDRSERQAIILNSLEKTCKTLGITLNYNNKLL
CCCCCCCCCEEEECHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHEEEEEECCCHHH
DEVTGLVEYPIVLYGTIPNEFLSLPEEIIITSVQTHQKYFTTKDHNNKLAPYFLFVTNIN
HHHHHHHHCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECC
TEDYSNIIKGNEKVLSSRLSDAIYFFNQDCQFTLLSKLEKLKKVVFHTKLGSIFDKVQRI
CHHHHHHHCCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKICHKLAPDDQELAIAAELCKSDLVSEVVQEFPNLQGIMGKYYALNDKLSPAVANIIGN
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCCHHHHHHHCC
HYHPTGIEDEVPNGKAAILALADKLDNLTSLYLAGERATSSKDPFALRRCAFSIARIILT
CCCCCCCCCCCCCCCEEHHHHHHHHCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHH
NKIQVKLLDLLTFIYQLHDIYTNELATEVLLFIKDKTKNLLNAKFNHLLVEAIFQQNNYD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DLTELELRMNILDHFLTSTKGQNFIQSYKRAYNIIKDEEIRQAKIDVCKLLTKQETILYQ
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
QAVDAKISQLFTQLSAETFSLLLDQLSNIGISIAKFLDNVPVNTTDQCLTYNRKLILQHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VQWCNNIAPFDKVIEHYVNKYEQ
HHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA