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The map label for this gene is nuoB

Identifier: 189183819

GI number: 189183819

Start: 954922

End: 955437

Strand: Direct

Name: nuoB

Synonym: OTT_0912

Alternate gene names: 189183819

Gene position: 954922-955437 (Clockwise)

Preceding gene: 189183818

Following gene: 189183820

Centisome position: 47.53

GC content: 35.66

Gene sequence:

>516_bases
ATGTCAGAAAATACATTTAGTGATACAACAAGTATCTATAATCAAAATTTATCTAATCAAGGATTTATCTTAGCTAATCT
AGAAAATTTAGTTAGCTGGGCTAGAACACGCTCGTTATGGCCTATGACATTTGGGTTAGCTTGCTGCGCTATAGAAATGA
TGCAAACAGCCGGTAGCAGATATGACATGGATCGTTATGGAATGTTATTTAGACCTAGTCCACGACAAGCTGATGTAATG
ATAGTAGCTGGAACACTAACTAATAAGATGGCACCAGCACTACGTAGAGTTTATGATCAAATGACTGAGCCAAAATGGGT
GATATCAATGGGTAGCTGTGCAAATGGAGGAGGATATTACCATTACTCTTATTCAGTAGTTAGAGGCTGTGATAAAATTG
TACCTGTAGATATTTATGTTCCTGGTTGTCCTCCTACCCCAGAAGCATTACTTTATGGTATTTTACAATTACAAAAAAAA
ATCAATAGAACTCAACAAGTCAAATTTAAAAGATAG

Upstream 100 bases:

>100_bases
TGGCTTTTATTCTATGATGTTTTTCCTACTGATATTAGTAATCGGATTTATCTATGAATGGAAAAAAGGTGCGTTAGAAT
GGGAGTAATTCAGGAGTTAA

Downstream 100 bases:

>100_bases
AGTGTAGTTTATGAAATTACATCACCTCAAAGAACTAAAAGAGCTAGTAGAAAGTCAATTTGCTATATCTAGCATTAGCG
CTAAGTTTAATATTACCAAT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MSENTFSDTTSIYNQNLSNQGFILANLENLVSWARTRSLWPMTFGLACCAIEMMQTAGSRYDMDRYGMLFRPSPRQADVM
IVAGTLTNKMAPALRRVYDQMTEPKWVISMGSCANGGGYYHYSYSVVRGCDKIVPVDIYVPGCPPTPEALLYGILQLQKK
INRTQQVKFKR

Sequences:

>Translated_171_residues
MSENTFSDTTSIYNQNLSNQGFILANLENLVSWARTRSLWPMTFGLACCAIEMMQTAGSRYDMDRYGMLFRPSPRQADVM
IVAGTLTNKMAPALRRVYDQMTEPKWVISMGSCANGGGYYHYSYSVVRGCDKIVPVDIYVPGCPPTPEALLYGILQLQKK
INRTQQVKFKR
>Mature_170_residues
SENTFSDTTSIYNQNLSNQGFILANLENLVSWARTRSLWPMTFGLACCAIEMMQTAGSRYDMDRYGMLFRPSPRQADVMI
VAGTLTNKMAPALRRVYDQMTEPKWVISMGSCANGGGYYHYSYSVVRGCDKIVPVDIYVPGCPPTPEALLYGILQLQKKI
NRTQQVKFKR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=168, Percent_Identity=67.8571428571429, Blast_Score=258, Evalue=2e-69,
Organism=Escherichia coli, GI1788624, Length=149, Percent_Identity=51.006711409396, Blast_Score=173, Evalue=5e-45,
Organism=Escherichia coli, GI1789074, Length=123, Percent_Identity=45.5284552845528, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=38.5245901639344, Blast_Score=99, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17509685, Length=152, Percent_Identity=69.7368421052632, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI18859983, Length=146, Percent_Identity=76.027397260274, Blast_Score=255, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24642371, Length=146, Percent_Identity=76.027397260274, Blast_Score=255, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24651058, Length=160, Percent_Identity=71.25, Blast_Score=250, Evalue=3e-67,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_ORITB (A5CES0)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001248970.1
- STRING:   A5CES0
- GeneID:   5218008
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_1628
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   GKPIGSN
- ProtClustDB:   PRK06411
- BioCyc:   OTSU357244:OTBS_1628-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 19331; Mature: 19199

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENTFSDTTSIYNQNLSNQGFILANLENLVSWARTRSLWPMTFGLACCAIEMMQTAGSR
CCCCCCCHHHHHHHCCCCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
YDMDRYGMLFRPSPRQADVMIVAGTLTNKMAPALRRVYDQMTEPKWVISMGSCANGGGYY
CCHHHCCEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEE
HYSYSVVRGCDKIVPVDIYVPGCPPTPEALLYGILQLQKKINRTQQVKFKR
EEEHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCHHHCCEECC
>Mature Secondary Structure 
SENTFSDTTSIYNQNLSNQGFILANLENLVSWARTRSLWPMTFGLACCAIEMMQTAGSR
CCCCCCHHHHHHHCCCCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
YDMDRYGMLFRPSPRQADVMIVAGTLTNKMAPALRRVYDQMTEPKWVISMGSCANGGGYY
CCHHHCCEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEE
HYSYSVVRGCDKIVPVDIYVPGCPPTPEALLYGILQLQKKINRTQQVKFKR
EEEHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA