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The map label for this gene is gyrB [H]

Identifier: 189183732

GI number: 189183732

Start: 864954

End: 867377

Strand: Direct

Name: gyrB [H]

Synonym: OTT_0825

Alternate gene names: 189183732

Gene position: 864954-867377 (Clockwise)

Preceding gene: 189183723

Following gene: 189183734

Centisome position: 43.05

GC content: 33.09

Gene sequence:

>2424_bases
ATGTCATCAAGCTTAAATATGCCAACAAACACAAAATTAGATCAATATAATGCTGATTCAATAAAGATCTTACGAGGTCT
TGAAGCGGTTCGAGTACGGCCTGGTATGTATATTGGTGATATTAGTAATGGATCTGGATTACATCAGTTAATTTATGAAG
TTCTAGATAATTCAACTGATGAGTCATTAGCTGGTGGTTGTAATAAAATTGAGGTTACTCTTAATAAAAATGGCTCTGTA
ACAGTCAGAGATAATGGCAGAGGAATACCTGTTGATATTCATCAAGAAGAGGGAGTATCTGCCGCTCAAGTCATTATGAC
CCAGTTGCACGCTGGAGGAAAATTTGATCAAAATGCATATAAAGTGTCAGGAGGATTACACGGAGTTGGAGTATCAGTAG
TTAATGCGTTATCAGATTGGTTAGAATTACGTATCTGGAAAAACAATCAAGAACATTTTATGCGTTTCAATAATGGAGAA
CCTCAAAATCCTTTATCGATAGTTGGTGAAGCTCCGAACAAGCAAGGTACAGAAATTACATTTTATCCTTCAATTGCAAT
TTTTACTTCTGTTGAGTTTAGCTTTTCAGAGTTAGAGCATAGATTAAGAGAATTAGCATTTTTAAATTCTTCTGTTGAAT
TTTCATTAACGGATGAGCGTTCTAGTGAAGTAAAAGATGTTAAATTTTTTTATACTGGTGGAGTAGAATCATATGTGCAA
TATATTGATAAAGCTAAGGCAGCATTGCATTCAACTATAAGCTTTCATCAAACTGATTCTCATGGAGCAACTTTAGAGTT
GGCTATGCAATGGAATGATTCTTTCTATGAAAATATAGTGTGTTTTACTAATAATATTAGACAAAGAGATGGTGGAAGCC
ATTTACAGGGATATAGATCAGCAGTAACTAGAGCTATCACTAAATACACTAAAGATCATTTTCCTAAAAATAATATCAAT
TATAATGGTGATGATATTCGAGAAGGGCTATCTTGTGTGTTATCATTAAAATTGCCTGATCCTAAATTTGCTTCACAAAC
TAAGGATAAGTTGGTTAGTCCGGAAGCTAGAGCTATAGTTGAAGGTATAGTATATGAAAAACTTGTAGAATGGTTAGAAC
ATCGCCCTAGTGAAGGTAAGAAGATTATTGCTAAAATTACTGAATCAGCGCTTGCACGTGAAGCAGCTCGTAAAGCTAGA
GAATTAACTAGACGTAAATCAGCATTAGGAGTAGCAAATCTACCAGGTAAATTAGCAGATTGTCAACAACGAGCTCCTGA
GAAATCTGAGTTATTTATAGTAGAAGGGGATTCAGCTGGAGGTACAGCTAAACAAGGGCGTAATAGAAAGTTTCAAGCTA
TTCTTCCACTAAGAGGCAAAGTATTAAATGTTGAAAGAGCTAGATTTGATAAGATGTTACAGTCTGATCAAATTGGCACT
TTAATTACTGCTCTTGGAACAGGAATCGGAAGTGATGACTTTGCTATTGATAAAATACGTTATCATAAAGTGGTTATTAT
GACTGATGCTGATGTTGATGGATCTCATATTCGAGCATTGCTTTTGACTTTCTTTTATCGTTATATGCCCACAGTTATTG
AAAAAGGTTATTTATATATTGCTCAACCTCCTTTATATAAAATTAAGCGTGGAGCTAATGAGTTGTATCTTAAAAACGAT
CAAGCATTATATGATTATTTATTAAAAATAGCTATTGATGAGATTGATGTAATCAAATCTTCTGGTGATAGAATATCGAA
TGCTGAGTTAACAACAATTGTTGCTTCGATTAGAAAGTTAACTGTAGTTTTAAATAAAGTAGGTAATAAACTAAATAAAT
ATATTGCCGAGGTTTTAGCTATTACCAGAAATCTTTCTGCAGATACTTTTAACTCTATCTATCAGGATAAATTATCATCT
CTTATACAACAAATGCTTCCAACTCAGACTTTAGATAAAACTAATTGGCAAGTTGAAGTTCATGATGATTATATGCAATT
TTATTGTTTTGTACGAGGGATAAAAAAAGCTCAAAGTCTTTTCAAAAGCCAATTGGAATCTCCAGAGTTTGTTAGTTTAA
TAAGGTTAGGCCAAGAATTATCTGAACTATTTGAAAATCGGTTAACATTAAAGCATAAATCAGAAGAAATTAATATCAGT
TTACCATCAGCTTTATTGGATAAATTATTAGCAATTGGTAAGCATGGAATGAGCATTCAAAGGTTTAAAGGGTTGGGAGA
GATGAATTCTGATCAGCTCTGGGAAACTACATTAGATCCAGAAAACAGAACGCTACTGCAGGTAAAAATCACTGATTTTG
ATGAAGCAGAAGAAGTATTTTCAACTTTAATGAGCAATATAGTAGAGCCTAGACGTGAGTTCATTCAAGCTAATGCTTTA
AATGTTAATAATTTAGATGTATAA

Upstream 100 bases:

>100_bases
GTAATATTAATTTAAGTATTTTTGACTTCTTAAAGATTATACTATATGTTATCATAATAGGTTTTTCCACTAAGTTATCC
ATTATAAAGTTGCAATTATT

Downstream 100 bases:

>100_bases
ATATAATGCCAGCAGTATATTTTTGCTGGCATTATAAATTATGCCTTACTGGCGACATTTGTTTTTCTTAAATGCTCTAT
AAAATATAGTACTGCATATA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 807; Mature: 806

Protein sequence:

>807_residues
MSSSLNMPTNTKLDQYNADSIKILRGLEAVRVRPGMYIGDISNGSGLHQLIYEVLDNSTDESLAGGCNKIEVTLNKNGSV
TVRDNGRGIPVDIHQEEGVSAAQVIMTQLHAGGKFDQNAYKVSGGLHGVGVSVVNALSDWLELRIWKNNQEHFMRFNNGE
PQNPLSIVGEAPNKQGTEITFYPSIAIFTSVEFSFSELEHRLRELAFLNSSVEFSLTDERSSEVKDVKFFYTGGVESYVQ
YIDKAKAALHSTISFHQTDSHGATLELAMQWNDSFYENIVCFTNNIRQRDGGSHLQGYRSAVTRAITKYTKDHFPKNNIN
YNGDDIREGLSCVLSLKLPDPKFASQTKDKLVSPEARAIVEGIVYEKLVEWLEHRPSEGKKIIAKITESALAREAARKAR
ELTRRKSALGVANLPGKLADCQQRAPEKSELFIVEGDSAGGTAKQGRNRKFQAILPLRGKVLNVERARFDKMLQSDQIGT
LITALGTGIGSDDFAIDKIRYHKVVIMTDADVDGSHIRALLLTFFYRYMPTVIEKGYLYIAQPPLYKIKRGANELYLKND
QALYDYLLKIAIDEIDVIKSSGDRISNAELTTIVASIRKLTVVLNKVGNKLNKYIAEVLAITRNLSADTFNSIYQDKLSS
LIQQMLPTQTLDKTNWQVEVHDDYMQFYCFVRGIKKAQSLFKSQLESPEFVSLIRLGQELSELFENRLTLKHKSEEINIS
LPSALLDKLLAIGKHGMSIQRFKGLGEMNSDQLWETTLDPENRTLLQVKITDFDEAEEVFSTLMSNIVEPRREFIQANAL
NVNNLDV

Sequences:

>Translated_807_residues
MSSSLNMPTNTKLDQYNADSIKILRGLEAVRVRPGMYIGDISNGSGLHQLIYEVLDNSTDESLAGGCNKIEVTLNKNGSV
TVRDNGRGIPVDIHQEEGVSAAQVIMTQLHAGGKFDQNAYKVSGGLHGVGVSVVNALSDWLELRIWKNNQEHFMRFNNGE
PQNPLSIVGEAPNKQGTEITFYPSIAIFTSVEFSFSELEHRLRELAFLNSSVEFSLTDERSSEVKDVKFFYTGGVESYVQ
YIDKAKAALHSTISFHQTDSHGATLELAMQWNDSFYENIVCFTNNIRQRDGGSHLQGYRSAVTRAITKYTKDHFPKNNIN
YNGDDIREGLSCVLSLKLPDPKFASQTKDKLVSPEARAIVEGIVYEKLVEWLEHRPSEGKKIIAKITESALAREAARKAR
ELTRRKSALGVANLPGKLADCQQRAPEKSELFIVEGDSAGGTAKQGRNRKFQAILPLRGKVLNVERARFDKMLQSDQIGT
LITALGTGIGSDDFAIDKIRYHKVVIMTDADVDGSHIRALLLTFFYRYMPTVIEKGYLYIAQPPLYKIKRGANELYLKND
QALYDYLLKIAIDEIDVIKSSGDRISNAELTTIVASIRKLTVVLNKVGNKLNKYIAEVLAITRNLSADTFNSIYQDKLSS
LIQQMLPTQTLDKTNWQVEVHDDYMQFYCFVRGIKKAQSLFKSQLESPEFVSLIRLGQELSELFENRLTLKHKSEEINIS
LPSALLDKLLAIGKHGMSIQRFKGLGEMNSDQLWETTLDPENRTLLQVKITDFDEAEEVFSTLMSNIVEPRREFIQANAL
NVNNLDV
>Mature_806_residues
SSSLNMPTNTKLDQYNADSIKILRGLEAVRVRPGMYIGDISNGSGLHQLIYEVLDNSTDESLAGGCNKIEVTLNKNGSVT
VRDNGRGIPVDIHQEEGVSAAQVIMTQLHAGGKFDQNAYKVSGGLHGVGVSVVNALSDWLELRIWKNNQEHFMRFNNGEP
QNPLSIVGEAPNKQGTEITFYPSIAIFTSVEFSFSELEHRLRELAFLNSSVEFSLTDERSSEVKDVKFFYTGGVESYVQY
IDKAKAALHSTISFHQTDSHGATLELAMQWNDSFYENIVCFTNNIRQRDGGSHLQGYRSAVTRAITKYTKDHFPKNNINY
NGDDIREGLSCVLSLKLPDPKFASQTKDKLVSPEARAIVEGIVYEKLVEWLEHRPSEGKKIIAKITESALAREAARKARE
LTRRKSALGVANLPGKLADCQQRAPEKSELFIVEGDSAGGTAKQGRNRKFQAILPLRGKVLNVERARFDKMLQSDQIGTL
ITALGTGIGSDDFAIDKIRYHKVVIMTDADVDGSHIRALLLTFFYRYMPTVIEKGYLYIAQPPLYKIKRGANELYLKNDQ
ALYDYLLKIAIDEIDVIKSSGDRISNAELTTIVASIRKLTVVLNKVGNKLNKYIAEVLAITRNLSADTFNSIYQDKLSSL
IQQMLPTQTLDKTNWQVEVHDDYMQFYCFVRGIKKAQSLFKSQLESPEFVSLIRLGQELSELFENRLTLKHKSEEINISL
PSALLDKLLAIGKHGMSIQRFKGLGEMNSDQLWETTLDPENRTLLQVKITDFDEAEEVFSTLMSNIVEPRREFIQANALN
VNNLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=576, Percent_Identity=24.3055555555556, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI19913408, Length=547, Percent_Identity=23.5831809872029, Blast_Score=122, Evalue=1e-27,
Organism=Escherichia coli, GI48994957, Length=805, Percent_Identity=49.4409937888199, Blast_Score=764, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=545, Percent_Identity=38.348623853211, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17535065, Length=579, Percent_Identity=24.5250431778929, Blast_Score=136, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI212645845, Length=515, Percent_Identity=25.0485436893204, Blast_Score=132, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI212645657, Length=144, Percent_Identity=29.8611111111111, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324241, Length=584, Percent_Identity=24.6575342465753, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17136538, Length=588, Percent_Identity=25.1700680272109, Blast_Score=131, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 90562; Mature: 90431

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSLNMPTNTKLDQYNADSIKILRGLEAVRVRPGMYIGDISNGSGLHQLIYEVLDNSTD
CCCCCCCCCCCCCCCCCCCHHHHHHCCHHHEECCCEEEEECCCCCCHHHHHHHHHCCCCC
ESLAGGCNKIEVTLNKNGSVTVRDNGRGIPVDIHQEEGVSAAQVIMTQLHAGGKFDQNAY
HHHHCCCCEEEEEECCCCEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCE
KVSGGLHGVGVSVVNALSDWLELRIWKNNQEHFMRFNNGEPQNPLSIVGEAPNKQGTEIT
EECCCCCHHHHHHHHHHHHHHEEEEECCCHHHHEEECCCCCCCCHHEEECCCCCCCCEEE
FYPSIAIFTSVEFSFSELEHRLRELAFLNSSVEFSLTDERSSEVKDVKFFYTGGVESYVQ
EECCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEECCHHHHHH
YIDKAKAALHSTISFHQTDSHGATLELAMQWNDSFYENIVCFTNNIRQRDGGSHLQGYRS
HHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCHHHHCEEEEECCCHHCCCCHHHHHHHH
AVTRAITKYTKDHFPKNNINYNGDDIREGLSCVLSLKLPDPKFASQTKDKLVSPEARAIV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHEEEEEECCCCCHHHHHHHHHCCCHHHHHH
EGIVYEKLVEWLEHRPSEGKKIIAKITESALAREAARKARELTRRKSALGVANLPGKLAD
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
CQQRAPEKSELFIVEGDSAGGTAKQGRNRKFQAILPLRGKVLNVERARFDKMLQSDQIGT
HHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCEECHHHHHHHHHHHCCCHHH
LITALGTGIGSDDFAIDKIRYHKVVIMTDADVDGSHIRALLLTFFYRYMPTVIEKGYLYI
HHHHHHCCCCCCCCEECEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
AQPPLYKIKRGANELYLKNDQALYDYLLKIAIDEIDVIKSSGDRISNAELTTIVASIRKL
ECCCHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
TVVLNKVGNKLNKYIAEVLAITRNLSADTFNSIYQDKLSSLIQQMLPTQTLDKTNWQVEV
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
HDDYMQFYCFVRGIKKAQSLFKSQLESPEFVSLIRLGQELSELFENRLTLKHKSEEINIS
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEE
LPSALLDKLLAIGKHGMSIQRFKGLGEMNSDQLWETTLDPENRTLLQVKITDFDEAEEVF
CHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCEECCCCCCCCEEEEEEECCCCCHHHHH
STLMSNIVEPRREFIQANALNVNNLDV
HHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SSSLNMPTNTKLDQYNADSIKILRGLEAVRVRPGMYIGDISNGSGLHQLIYEVLDNSTD
CCCCCCCCCCCCCCCCCCHHHHHHCCHHHEECCCEEEEECCCCCCHHHHHHHHHCCCCC
ESLAGGCNKIEVTLNKNGSVTVRDNGRGIPVDIHQEEGVSAAQVIMTQLHAGGKFDQNAY
HHHHCCCCEEEEEECCCCEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCE
KVSGGLHGVGVSVVNALSDWLELRIWKNNQEHFMRFNNGEPQNPLSIVGEAPNKQGTEIT
EECCCCCHHHHHHHHHHHHHHEEEEECCCHHHHEEECCCCCCCCHHEEECCCCCCCCEEE
FYPSIAIFTSVEFSFSELEHRLRELAFLNSSVEFSLTDERSSEVKDVKFFYTGGVESYVQ
EECCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEECCHHHHHH
YIDKAKAALHSTISFHQTDSHGATLELAMQWNDSFYENIVCFTNNIRQRDGGSHLQGYRS
HHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCHHHHCEEEEECCCHHCCCCHHHHHHHH
AVTRAITKYTKDHFPKNNINYNGDDIREGLSCVLSLKLPDPKFASQTKDKLVSPEARAIV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHEEEEEECCCCCHHHHHHHHHCCCHHHHHH
EGIVYEKLVEWLEHRPSEGKKIIAKITESALAREAARKARELTRRKSALGVANLPGKLAD
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
CQQRAPEKSELFIVEGDSAGGTAKQGRNRKFQAILPLRGKVLNVERARFDKMLQSDQIGT
HHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCEECHHHHHHHHHHHCCCHHH
LITALGTGIGSDDFAIDKIRYHKVVIMTDADVDGSHIRALLLTFFYRYMPTVIEKGYLYI
HHHHHHCCCCCCCCEECEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
AQPPLYKIKRGANELYLKNDQALYDYLLKIAIDEIDVIKSSGDRISNAELTTIVASIRKL
ECCCHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
TVVLNKVGNKLNKYIAEVLAITRNLSADTFNSIYQDKLSSLIQQMLPTQTLDKTNWQVEV
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
HDDYMQFYCFVRGIKKAQSLFKSQLESPEFVSLIRLGQELSELFENRLTLKHKSEEINIS
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEE
LPSALLDKLLAIGKHGMSIQRFKGLGEMNSDQLWETTLDPENRTLLQVKITDFDEAEEVF
CHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCEECCCCCCCCEEEEEEECCCCCHHHHH
STLMSNIVEPRREFIQANALNVNNLDV
HHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA