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The map label for this gene is gltX

Identifier: 189183684

GI number: 189183684

Start: 817767

End: 819113

Strand: Direct

Name: gltX

Synonym: OTT_0777

Alternate gene names: 189183684

Gene position: 817767-819113 (Clockwise)

Preceding gene: 189183683

Following gene: 189183685

Centisome position: 40.71

GC content: 32.29

Gene sequence:

>1347_bases
ATGACTGTAATTACTAGATTTGCTCCTTCTCCTACAGGAAAATTACATATTGGCAATGTTAGAGTTGCTTTAGTAAATTG
GTTGTATGCCCAAAAGTATAATGGGAATTTTATTTTACGTATTGATGACACTGATCGTGACCGTAGTAAAGTAGAGTACC
ATGAAGCTATTATTAAAGATTTGCAGTGGTTAGGAATAAATTGGAATAGCAGCTTTTTACAGTCTACTAGATTCAATAAA
TATGCAGCAGCTAAGCAATATTTAATTAGTAGTGGTAGATTATATGAATGTTATGAAACGCCTGAAATGCTAGAAATAGA
GCGAAAGAGACAGCTTGCATCTGGGTATCCGCCAATCTATAGCAGAAAATCTCTTGAACTAACAACAGCACAGAAGGTGC
AATTGCAAGCTGAAGGATATAAAGTTCATTATCGATTTTTAATTGATCGTAATAAACCAATAATTTGGAATGATTTAATT
AAGGGAGAAATCAAATATGATGGTTCAAATGTTAGTGATCCGATAGTTATAAAAGAAGATGGTACCATGATCTATATGTT
ATGTTCTGTTATTGATGATATAGAATATCGCATTAGTCATATAATACGTGGCGAAGATCATATTACTAATACAGCTATTC
AAATTCAGATGTTTGAAGCATTAGGCGCTGCAATACCTCAATTAGGACATCTAAGTTTAATTAAGTCAGATAGTGGTAAA
ATTTCTAAAAGAATAGGTGGCTTTACAATCGATTACCTCAAAGATCAGTTGGGTATTGAACCAATGGCTGTTAATAATTT
ATTGGCGTTGTCTGGCACATCAAATAACGTTGATGCATATTTCAGTTTAGAATCACTTATCACTAAGTTTGATCTTAGTG
CATTTTCAAAAAGTACAATTATTTATAATGAAAATGAGCTTGTTACGCTAAATCATAAGCTTCTTGTAAACACTGAGTAT
GATACCATTAAACATAGGTTAACTGCAATTGGATTACCTGATGTTACTAAAGAATTTTGGTTAGCGGTACGCCATAATCT
GAATACATTAAATGATATCAAGATTTGGTGGCAAATATGCTATTTACCTACTTTAGATAAATTTCAGGAGCAGGATGCAG
AGTTTTTAAAGCTTGCTGCTGAATTGTTGCCTTCAGGAAAGTTAACTGATAATAGTTGGGATGATTGGGTTCAAAATATT
ATTAAAGCAACTAATAGACGTGGTAAAGCATTATTTATGCCTTTAAGATTAGCTCTAACTGGTATAACATATGGCCCTGA
ATTAAAATATTTATTACCTTTAATTGGAGGAGATGAAGTTAGAGCAAGACTTCTAAGATATCAATAA

Upstream 100 bases:

>100_bases
CAATTCTAAGTTAAATCAGTATAGGTATTGCAATAATGTCATAATCTTGCATAATAAATATCCATTAGCATACTTTTTTG
ATAATTTAAATTTAGAAAAA

Downstream 100 bases:

>100_bases
ACAAGAAAATTTAACAATTCAGGTACTCATATAATTAAGGACGATAAATATTAGAATATAGTATATGAACTTATATATTT
ATAACACCCTTTCTAGGAAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MTVITRFAPSPTGKLHIGNVRVALVNWLYAQKYNGNFILRIDDTDRDRSKVEYHEAIIKDLQWLGINWNSSFLQSTRFNK
YAAAKQYLISSGRLYECYETPEMLEIERKRQLASGYPPIYSRKSLELTTAQKVQLQAEGYKVHYRFLIDRNKPIIWNDLI
KGEIKYDGSNVSDPIVIKEDGTMIYMLCSVIDDIEYRISHIIRGEDHITNTAIQIQMFEALGAAIPQLGHLSLIKSDSGK
ISKRIGGFTIDYLKDQLGIEPMAVNNLLALSGTSNNVDAYFSLESLITKFDLSAFSKSTIIYNENELVTLNHKLLVNTEY
DTIKHRLTAIGLPDVTKEFWLAVRHNLNTLNDIKIWWQICYLPTLDKFQEQDAEFLKLAAELLPSGKLTDNSWDDWVQNI
IKATNRRGKALFMPLRLALTGITYGPELKYLLPLIGGDEVRARLLRYQ

Sequences:

>Translated_448_residues
MTVITRFAPSPTGKLHIGNVRVALVNWLYAQKYNGNFILRIDDTDRDRSKVEYHEAIIKDLQWLGINWNSSFLQSTRFNK
YAAAKQYLISSGRLYECYETPEMLEIERKRQLASGYPPIYSRKSLELTTAQKVQLQAEGYKVHYRFLIDRNKPIIWNDLI
KGEIKYDGSNVSDPIVIKEDGTMIYMLCSVIDDIEYRISHIIRGEDHITNTAIQIQMFEALGAAIPQLGHLSLIKSDSGK
ISKRIGGFTIDYLKDQLGIEPMAVNNLLALSGTSNNVDAYFSLESLITKFDLSAFSKSTIIYNENELVTLNHKLLVNTEY
DTIKHRLTAIGLPDVTKEFWLAVRHNLNTLNDIKIWWQICYLPTLDKFQEQDAEFLKLAAELLPSGKLTDNSWDDWVQNI
IKATNRRGKALFMPLRLALTGITYGPELKYLLPLIGGDEVRARLLRYQ
>Mature_447_residues
TVITRFAPSPTGKLHIGNVRVALVNWLYAQKYNGNFILRIDDTDRDRSKVEYHEAIIKDLQWLGINWNSSFLQSTRFNKY
AAAKQYLISSGRLYECYETPEMLEIERKRQLASGYPPIYSRKSLELTTAQKVQLQAEGYKVHYRFLIDRNKPIIWNDLIK
GEIKYDGSNVSDPIVIKEDGTMIYMLCSVIDDIEYRISHIIRGEDHITNTAIQIQMFEALGAAIPQLGHLSLIKSDSGKI
SKRIGGFTIDYLKDQLGIEPMAVNNLLALSGTSNNVDAYFSLESLITKFDLSAFSKSTIIYNENELVTLNHKLLVNTEYD
TIKHRLTAIGLPDVTKEFWLAVRHNLNTLNDIKIWWQICYLPTLDKFQEQDAEFLKLAAELLPSGKLTDNSWDDWVQNII
KATNRRGKALFMPLRLALTGITYGPELKYLLPLIGGDEVRARLLRYQ

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=485, Percent_Identity=28.0412371134021, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI62241042, Length=246, Percent_Identity=24.7967479674797, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788743, Length=468, Percent_Identity=33.5470085470085, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI226510925, Length=257, Percent_Identity=29.1828793774319, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1786895, Length=170, Percent_Identity=34.1176470588235, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71993394, Length=342, Percent_Identity=33.6257309941521, Blast_Score=183, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324540, Length=486, Percent_Identity=29.0123456790123, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6321192, Length=148, Percent_Identity=35.1351351351351, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324742, Length=136, Percent_Identity=35.2941176470588, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24665321, Length=484, Percent_Identity=28.9256198347107, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24649466, Length=211, Percent_Identity=28.436018957346, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_ORITI (B3CRX8)

Other databases:

- EMBL:   AP008981
- RefSeq:   YP_001937469.1
- GeneID:   6337915
- GenomeReviews:   AP008981_GR
- KEGG:   ott:OTT_0777
- HOGENOM:   HBG628189
- OMA:   RSKQEYI
- ProtClustDB:   PRK12558
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51380; Mature: 51249

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 243-243

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVITRFAPSPTGKLHIGNVRVALVNWLYAQKYNGNFILRIDDTDRDRSKVEYHEAIIKD
CCEEEECCCCCCCEEEECCEEEEEHHHHHHEEECCEEEEEECCCCCCHHHHHHHHHHHHH
LQWLGINWNSSFLQSTRFNKYAAAKQYLISSGRLYECYETPEMLEIERKRQLASGYPPIY
HHEEECCCCHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCCC
SRKSLELTTAQKVQLQAEGYKVHYRFLIDRNKPIIWNDLIKGEIKYDGSNVSDPIVIKED
CCCCEEEEEEEEEEEEECCEEEEEEEEEECCCCEEEECCCCCEEEECCCCCCCCEEEECC
GTMIYMLCSVIDDIEYRISHIIRGEDHITNTAIQIQMFEALGAAIPQLGHLSLIKSDSGK
CCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHCCCCCCEEEEECCCCH
ISKRIGGFTIDYLKDQLGIEPMAVNNLLALSGTSNNVDAYFSLESLITKFDLSAFSKSTI
HHHHHCCEEHHHHHHHHCCCCEEECCEEEEECCCCCCEEEEEHHHHHHHHHHHHCCCEEE
IYNENELVTLNHKLLVNTEYDTIKHRLTAIGLPDVTKEFWLAVRHNLNTLNDIKIWWQIC
EECCCCEEEEEEEEEEECCHHHHHHHHHEECCCCHHHHHHHHHHHCCCCCHHHEEEEEEE
YLPTLDKFQEQDAEFLKLAAELLPSGKLTDNSWDDWVQNIIKATNRRGKALFMPLRLALT
ECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHHC
GITYGPELKYLLPLIGGDEVRARLLRYQ
CCCCCCCCCEEEEECCCHHHHHHHHCCC
>Mature Secondary Structure 
TVITRFAPSPTGKLHIGNVRVALVNWLYAQKYNGNFILRIDDTDRDRSKVEYHEAIIKD
CEEEECCCCCCCEEEECCEEEEEHHHHHHEEECCEEEEEECCCCCCHHHHHHHHHHHHH
LQWLGINWNSSFLQSTRFNKYAAAKQYLISSGRLYECYETPEMLEIERKRQLASGYPPIY
HHEEECCCCHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCCC
SRKSLELTTAQKVQLQAEGYKVHYRFLIDRNKPIIWNDLIKGEIKYDGSNVSDPIVIKED
CCCCEEEEEEEEEEEEECCEEEEEEEEEECCCCEEEECCCCCEEEECCCCCCCCEEEECC
GTMIYMLCSVIDDIEYRISHIIRGEDHITNTAIQIQMFEALGAAIPQLGHLSLIKSDSGK
CCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHCCCCCCEEEEECCCCH
ISKRIGGFTIDYLKDQLGIEPMAVNNLLALSGTSNNVDAYFSLESLITKFDLSAFSKSTI
HHHHHCCEEHHHHHHHHCCCCEEECCEEEEECCCCCCEEEEEHHHHHHHHHHHHCCCEEE
IYNENELVTLNHKLLVNTEYDTIKHRLTAIGLPDVTKEFWLAVRHNLNTLNDIKIWWQIC
EECCCCEEEEEEEEEEECCHHHHHHHHHEECCCCHHHHHHHHHHHCCCCCHHHEEEEEEE
YLPTLDKFQEQDAEFLKLAAELLPSGKLTDNSWDDWVQNIIKATNRRGKALFMPLRLALT
ECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHHC
GITYGPELKYLLPLIGGDEVRARLLRYQ
CCCCCCCCCEEEEECCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA