Click here to switch to the map view.

The map label for this gene is spoT [H]

Identifier: 189183666

GI number: 189183666

Start: 786181

End: 786756

Strand: Direct

Name: spoT [H]

Synonym: OTT_0759

Alternate gene names: 189183666

Gene position: 786181-786756 (Clockwise)

Preceding gene: 189183665

Following gene: 189183667

Centisome position: 39.13

GC content: 31.08

Gene sequence:

>576_bases
ATGATAGATTTTTATAGTGAAAGCTTAATAAATAAGCTGTTCAGAACCAACGTAAGATTTAACACCAAAATTGATCTTGA
TAAAGTTGAAAGAGCAATAAAATATGCTAAAAAATATCATGGCCAGCAAAAGAGAGATACTGGAGAACTCTACTACACAC
ATCCATTGAAAGTAGCGTACATGGTATCAGACTACAGCTTTGAAACAGATACAATTATTACTGCAATACTACATGATACA
CTTGAAGACACAAAACTAACTAAAGAAAGAATAAGGTACGAATTTGGTGCTAATATTGCAGAACAGGTTTCAGACCTTAC
CAGAGTTAGGGATAATAAGAAAATCAGTGCTATGGAAATGATACAAATATTACGCAGCCAAAATAAAACAGAACTATTAC
TGATCAAGCTTTTTGATAGATTCCATAATATTCAAACCATACATATAAAACCTTATGGAAAAAGACAAAAAATCGTCATT
GAAACTCAGCAAGAATTTATACCTCTTGCTCAATATCTTAAATTACCAGAAATTACCATAGAGCTAAATAAATACTGTGA
GCTTTATGTTAGTTAG

Upstream 100 bases:

>100_bases
ATAATTTAATTTGCTTCTTTTCCAGTTTTTATCTGGGATTGGAGTATACAAAAAGTTTAAATTATGGTATAATTTATTAA
TAACTATTTAGAGGTGGCCA

Downstream 100 bases:

>100_bases
AATGCAAACTTTTTCTAACTAGCAAACATAGCATTAATATATATTTCGCTTTGCAGAATCAAGCATAGCTGCTTGGCATA
GATATATTATCGTTCAATTA

Product: RelA/SpoT-related protein

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MIDFYSESLINKLFRTNVRFNTKIDLDKVERAIKYAKKYHGQQKRDTGELYYTHPLKVAYMVSDYSFETDTIITAILHDT
LEDTKLTKERIRYEFGANIAEQVSDLTRVRDNKKISAMEMIQILRSQNKTELLLIKLFDRFHNIQTIHIKPYGKRQKIVI
ETQQEFIPLAQYLKLPEITIELNKYCELYVS

Sequences:

>Translated_191_residues
MIDFYSESLINKLFRTNVRFNTKIDLDKVERAIKYAKKYHGQQKRDTGELYYTHPLKVAYMVSDYSFETDTIITAILHDT
LEDTKLTKERIRYEFGANIAEQVSDLTRVRDNKKISAMEMIQILRSQNKTELLLIKLFDRFHNIQTIHIKPYGKRQKIVI
ETQQEFIPLAQYLKLPEITIELNKYCELYVS
>Mature_191_residues
MIDFYSESLINKLFRTNVRFNTKIDLDKVERAIKYAKKYHGQQKRDTGELYYTHPLKVAYMVSDYSFETDTIITAILHDT
LEDTKLTKERIRYEFGANIAEQVSDLTRVRDNKKISAMEMIQILRSQNKTELLLIKLFDRFHNIQTIHIKPYGKRQKIVI
ETQQEFIPLAQYLKLPEITIELNKYCELYVS

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=169, Percent_Identity=33.1360946745562, Blast_Score=89, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 22736; Mature: 22736

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDFYSESLINKLFRTNVRFNTKIDLDKVERAIKYAKKYHGQQKRDTGELYYTHPLKVAY
CCCCHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEE
MVSDYSFETDTIITAILHDTLEDTKLTKERIRYEFGANIAEQVSDLTRVRDNKKISAMEM
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHH
IQILRSQNKTELLLIKLFDRFHNIQTIHIKPYGKRQKIVIETQQEFIPLAQYLKLPEITI
HHHHHCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCEEEEECHHHHHHHHHHHCCCCEEE
ELNKYCELYVS
EECCEEEEECC
>Mature Secondary Structure
MIDFYSESLINKLFRTNVRFNTKIDLDKVERAIKYAKKYHGQQKRDTGELYYTHPLKVAY
CCCCHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEE
MVSDYSFETDTIITAILHDTLEDTKLTKERIRYEFGANIAEQVSDLTRVRDNKKISAMEM
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHH
IQILRSQNKTELLLIKLFDRFHNIQTIHIKPYGKRQKIVIETQQEFIPLAQYLKLPEITI
HHHHHCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCEEEEECHHHHHHHHHHHCCCCEEE
ELNKYCELYVS
EECCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]