Click here to switch to the map view.

The map label for this gene is dnaB [H]

Identifier: 189183665

GI number: 189183665

Start: 785152

End: 785943

Strand: Direct

Name: dnaB [H]

Synonym: OTT_0758

Alternate gene names: 189183665

Gene position: 785152-785943 (Clockwise)

Preceding gene: 189183664

Following gene: 189183666

Centisome position: 39.08

GC content: 31.94

Gene sequence:

>792_bases
ATGGGTAAAACTGCTTTAGGAGTTAACTTAGCAATAAATGCTTGTAAATATTTTCTTACTCAAAAAAATACTAAAGATAA
TGTAGTACCATCAGTTGGCTTCTTTTCTTTAGAAATGTCATCTCAGCAAATCTCAACTCGAATTCTTTCTATAGAATCAG
AAATTAATAGCTCTGCATTATTTAACGGTAAAATAAATGAACAAGATGTTGATAAGTTAAAGACTGTACAAGACGAAATA
CAAAAGTGGAATTTTTTTATAGATGATGCTCCAGCAATCTCGATATCTGCAATTAGATCTCGAGCTCGTAGACTTAAACG
TACTCATAATTTAGCAATATTATTTATTGATTATTTACAGCTAATAAAAATTGATTCCAGAGGAAGTCAGTATAATCGAG
TACAGGAGATTTCTGAAATTACTCAGAGCTTAAAAGCTCTTGCAAAAGAACTTAATATTTCAATAATTGCGTTATCTCAA
TTATCTAGAGCTGTAGAACAAAGGACAGATAAAAAGCCTATTCTCTCAGATCTACGAGAATCAGGTTCAATTGAACAGGA
TGCAGATATTGTAATGCTCATATATCGGGACGAATATTATCTATCTAGATCAGAACCAGATCCAGGTACTCGAGAACACA
TAGAATGGAAAGCTAGACAAAATAAATGTTATAACACTGCTGAAATAATTGTTGCTAAACACCGTAATGGTCCGGTTGGT
ACAGTGAAGTTGCACTATAATAGTAGGTATTCTAAATTTGGCAATATTGTTAAAAACTCTCAGCAATGTTAA

Upstream 100 bases:

>100_bases
AAACTCTATTAACGGTATTAGTAGTGGACTACTTGACCTTGATTCAAAGCTTGGAGGATTTAAAAATTCTGACCTAATAA
TATTAGCTGGCAGACCTTCA

Downstream 100 bases:

>100_bases
TAAACGCTAGATTATGCATAAGATGAAAAAATGCTTATCCAAAAAAAAAATTACAGCAAAATTTTACTCATTAACACCAG
AGATATGCAACTCAAGATTG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MGKTALGVNLAINACKYFLTQKNTKDNVVPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKINEQDVDKLKTVQDEI
QKWNFFIDDAPAISISAIRSRARRLKRTHNLAILFIDYLQLIKIDSRGSQYNRVQEISEITQSLKALAKELNISIIALSQ
LSRAVEQRTDKKPILSDLRESGSIEQDADIVMLIYRDEYYLSRSEPDPGTREHIEWKARQNKCYNTAEIIVAKHRNGPVG
TVKLHYNSRYSKFGNIVKNSQQC

Sequences:

>Translated_263_residues
MGKTALGVNLAINACKYFLTQKNTKDNVVPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKINEQDVDKLKTVQDEI
QKWNFFIDDAPAISISAIRSRARRLKRTHNLAILFIDYLQLIKIDSRGSQYNRVQEISEITQSLKALAKELNISIIALSQ
LSRAVEQRTDKKPILSDLRESGSIEQDADIVMLIYRDEYYLSRSEPDPGTREHIEWKARQNKCYNTAEIIVAKHRNGPVG
TVKLHYNSRYSKFGNIVKNSQQC
>Mature_262_residues
GKTALGVNLAINACKYFLTQKNTKDNVVPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKINEQDVDKLKTVQDEIQ
KWNFFIDDAPAISISAIRSRARRLKRTHNLAILFIDYLQLIKIDSRGSQYNRVQEISEITQSLKALAKELNISIIALSQL
SRAVEQRTDKKPILSDLRESGSIEQDADIVMLIYRDEYYLSRSEPDPGTREHIEWKARQNKCYNTAEIIVAKHRNGPVGT
VKLHYNSRYSKFGNIVKNSQQC

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=257, Percent_Identity=43.1906614785992, Blast_Score=201, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 29945; Mature: 29814

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTALGVNLAINACKYFLTQKNTKDNVVPSVGFFSLEMSSQQISTRILSIESEINSSAL
CCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEE
FNGKINEQDVDKLKTVQDEIQKWNFFIDDAPAISISAIRSRARRLKRTHNLAILFIDYLQ
ECCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHCCEEEHHHHHHH
LIKIDSRGSQYNRVQEISEITQSLKALAKELNISIIALSQLSRAVEQRTDKKPILSDLRE
HHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEHHHHHHHHHHHCCCCHHHHHHHH
SGSIEQDADIVMLIYRDEYYLSRSEPDPGTREHIEWKARQNKCYNTAEIIVAKHRNGPVG
CCCCCCCCCEEEEEEECCEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCEE
TVKLHYNSRYSKFGNIVKNSQQC
EEEEEECCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
GKTALGVNLAINACKYFLTQKNTKDNVVPSVGFFSLEMSSQQISTRILSIESEINSSAL
CCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEE
FNGKINEQDVDKLKTVQDEIQKWNFFIDDAPAISISAIRSRARRLKRTHNLAILFIDYLQ
ECCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHCCEEEHHHHHHH
LIKIDSRGSQYNRVQEISEITQSLKALAKELNISIIALSQLSRAVEQRTDKKPILSDLRE
HHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEHHHHHHHHHHHCCCCHHHHHHHH
SGSIEQDADIVMLIYRDEYYLSRSEPDPGTREHIEWKARQNKCYNTAEIIVAKHRNGPVG
CCCCCCCCCEEEEEEECCEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCEE
TVKLHYNSRYSKFGNIVKNSQQC
EEEEEECCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA