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The map label for this gene is spl1 [H]

Identifier: 189183481

GI number: 189183481

Start: 586267

End: 587553

Strand: Reverse

Name: spl1 [H]

Synonym: OTT_0574

Alternate gene names: 189183481

Gene position: 587553-586267 (Counterclockwise)

Preceding gene: 189183482

Following gene: 189183480

Centisome position: 29.25

GC content: 32.25

Gene sequence:

>1287_bases
ATGTATAACTTTATTGATCATAGCACTGCAGCAGCTATTGAAAATTCAGCTAGATATTATAATTCTACTGATAGGCCTTC
TTTTTCTAGTTTCAACATAGATAACCTAGTGAAAACAAAACAAAAAAATAAAAAAGTATATTTTGATTACAACGCTACAA
CTCCTATACATTCAGAGTCGCTAGAAGTTATGTTAGAAGTGTTACATATGCCAGCTAACCCTTCTTCGATACACTATTTT
GGACAGATAGCTAAAGGGTTTATAGAAAAAGCTAGATTGCAAATTGCTAATACATTAGATATATCATTAAGTCATAACAA
ATTTAATTGCATATTTGTTTCTTCAGGTACTGAAGCAAATAATTTACTGCTTACTAGCTTTAAAAAAGTTTATAAGAATG
GAATAATTATTACCTGTGCAACAGAGCATCTATCAATATTAAATTGTGCTAAAAGTTATACTGACACGCAAATTATAGAA
GTAGATGAAAGTGGTATTGTTGATCTTATACAGTTACAAAATACGCTTTATAGTAATAAAGGCTTGCCTTTGTTAGTTAC
AATTATGTTGGTAAATAACGAAACTGGTATTATACAGCCACTTGAAGAGATAGTTAGAATATCTAAAGAATATCAGGCAT
GGGTTCATAGCGATTGCAGCCAAGCCTTTGCTAAGATTCCAGTTAATCTCACTCAGTTAAATCTTGATTTTGCAACAATT
TCAAGCCACAAAATTGGAGGACCAATAGGTGCTGCGTCTCTTATAGCAAAATCTCCAAATTTTATTACACCACAAATGGT
TGGCGGAGGTCAAGAAAATGGGATTAGAGCCGGCACTGAAAATGTTGCGGCAATAGCTGGATTTGGCAAAGCTGTTGAGC
TTTTACCTAAAATGCTGAAACAAAGTAATTTTACTAAAACCTTAAGACAATATCTTGAAGACAAAATTAGCAGAATAGAT
AGTAAGATAAAAATTTTTGGCCAAAATGCTCCCAGAGTTGGTAATACTAGCATGATAATGATGCCAGAGGTTACATCTCA
ACAACAAATTCTTTATTTTGATATTAATGGATTTGCTGTGAGTGTTGGTTCAGCATGTTCTTCAGGTCAGCTAAAACAAT
CTTATGTACTTACAGCTATGAAGATTCCAGCTCATGAAGCAGACTGTGCAATTAGAATATCTCTTGGATGGCATAACACT
GAAGAAGAAATAAAACAATTTTATACATTATGGCAAAAACAATATTTATATCATATCAATGCTAGTAATATAAAGAAACA
AGCTTAA

Upstream 100 bases:

>100_bases
ACTACTTTAGTGAAATCTCTTTGGATGATATAATTTCCGGGAAAGTAAAGTCACAAGCTTTAAAAGCAAGATCACTTAGG
TTAAATTGAATGGCTGAGTA

Downstream 100 bases:

>100_bases
ATCAAATAAATATTATGATAAACACAGTAACAAAAACAGCAGAGTTAAGGTCAAAATTAAAAAACAATAATGTTAAGTTT
CCTATTTATATGGACTATCA

Product: NifS protein homolog

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MYNFIDHSTAAAIENSARYYNSTDRPSFSSFNIDNLVKTKQKNKKVYFDYNATTPIHSESLEVMLEVLHMPANPSSIHYF
GQIAKGFIEKARLQIANTLDISLSHNKFNCIFVSSGTEANNLLLTSFKKVYKNGIIITCATEHLSILNCAKSYTDTQIIE
VDESGIVDLIQLQNTLYSNKGLPLLVTIMLVNNETGIIQPLEEIVRISKEYQAWVHSDCSQAFAKIPVNLTQLNLDFATI
SSHKIGGPIGAASLIAKSPNFITPQMVGGGQENGIRAGTENVAAIAGFGKAVELLPKMLKQSNFTKTLRQYLEDKISRID
SKIKIFGQNAPRVGNTSMIMMPEVTSQQQILYFDINGFAVSVGSACSSGQLKQSYVLTAMKIPAHEADCAIRISLGWHNT
EEEIKQFYTLWQKQYLYHINASNIKKQA

Sequences:

>Translated_428_residues
MYNFIDHSTAAAIENSARYYNSTDRPSFSSFNIDNLVKTKQKNKKVYFDYNATTPIHSESLEVMLEVLHMPANPSSIHYF
GQIAKGFIEKARLQIANTLDISLSHNKFNCIFVSSGTEANNLLLTSFKKVYKNGIIITCATEHLSILNCAKSYTDTQIIE
VDESGIVDLIQLQNTLYSNKGLPLLVTIMLVNNETGIIQPLEEIVRISKEYQAWVHSDCSQAFAKIPVNLTQLNLDFATI
SSHKIGGPIGAASLIAKSPNFITPQMVGGGQENGIRAGTENVAAIAGFGKAVELLPKMLKQSNFTKTLRQYLEDKISRID
SKIKIFGQNAPRVGNTSMIMMPEVTSQQQILYFDINGFAVSVGSACSSGQLKQSYVLTAMKIPAHEADCAIRISLGWHNT
EEEIKQFYTLWQKQYLYHINASNIKKQA
>Mature_428_residues
MYNFIDHSTAAAIENSARYYNSTDRPSFSSFNIDNLVKTKQKNKKVYFDYNATTPIHSESLEVMLEVLHMPANPSSIHYF
GQIAKGFIEKARLQIANTLDISLSHNKFNCIFVSSGTEANNLLLTSFKKVYKNGIIITCATEHLSILNCAKSYTDTQIIE
VDESGIVDLIQLQNTLYSNKGLPLLVTIMLVNNETGIIQPLEEIVRISKEYQAWVHSDCSQAFAKIPVNLTQLNLDFATI
SSHKIGGPIGAASLIAKSPNFITPQMVGGGQENGIRAGTENVAAIAGFGKAVELLPKMLKQSNFTKTLRQYLEDKISRID
SKIKIFGQNAPRVGNTSMIMMPEVTSQQQILYFDINGFAVSVGSACSSGQLKQSYVLTAMKIPAHEADCAIRISLGWHNT
EEEIKQFYTLWQKQYLYHINASNIKKQA

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=372, Percent_Identity=31.1827956989247, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI156713448, Length=425, Percent_Identity=31.5294117647059, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI48994898, Length=384, Percent_Identity=32.8125, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI1787970, Length=330, Percent_Identity=26.0606060606061, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25143064, Length=380, Percent_Identity=32.1052631578947, Blast_Score=177, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI17533177, Length=308, Percent_Identity=33.7662337662338, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6319831, Length=372, Percent_Identity=31.989247311828, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI20129463, Length=386, Percent_Identity=31.6062176165803, Blast_Score=180, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 47568; Mature: 47568

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNFIDHSTAAAIENSARYYNSTDRPSFSSFNIDNLVKTKQKNKKVYFDYNATTPIHSES
CCCCCCCCHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHH
LEVMLEVLHMPANPSSIHYFGQIAKGFIEKARLQIANTLDISLSHNKFNCIFVSSGTEAN
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCEEEEEEECCCCCC
NLLLTSFKKVYKNGIIITCATEHLSILNCAKSYTDTQIIEVDESGIVDLIQLQNTLYSNK
HHHHHHHHHHHHCCEEEEECHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCC
GLPLLVTIMLVNNETGIIQPLEEIVRISKEYQAWVHSDCSQAFAKIPVNLTQLNLDFATI
CCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEE
SSHKIGGPIGAASLIAKSPNFITPQMVGGGQENGIRAGTENVAAIAGFGKAVELLPKMLK
CCCCCCCCCCHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCCEEHHHCCCHHHHHHHHHHH
QSNFTKTLRQYLEDKISRIDSKIKIFGQNAPRVGNTSMIMMPEVTSQQQILYFDINGFAV
CCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCCCEEEEEEECCEEE
SVGSACSSGQLKQSYVLTAMKIPAHEADCAIRISLGWHNTEEEIKQFYTLWQKQYLYHIN
EECCCCCCCCCCHHEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHEEEEEC
ASNIKKQA
HHHCCCCC
>Mature Secondary Structure
MYNFIDHSTAAAIENSARYYNSTDRPSFSSFNIDNLVKTKQKNKKVYFDYNATTPIHSES
CCCCCCCCHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHH
LEVMLEVLHMPANPSSIHYFGQIAKGFIEKARLQIANTLDISLSHNKFNCIFVSSGTEAN
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCEEEEEEECCCCCC
NLLLTSFKKVYKNGIIITCATEHLSILNCAKSYTDTQIIEVDESGIVDLIQLQNTLYSNK
HHHHHHHHHHHHCCEEEEECHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCC
GLPLLVTIMLVNNETGIIQPLEEIVRISKEYQAWVHSDCSQAFAKIPVNLTQLNLDFATI
CCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEE
SSHKIGGPIGAASLIAKSPNFITPQMVGGGQENGIRAGTENVAAIAGFGKAVELLPKMLK
CCCCCCCCCCHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCCEEHHHCCCHHHHHHHHHHH
QSNFTKTLRQYLEDKISRIDSKIKIFGQNAPRVGNTSMIMMPEVTSQQQILYFDINGFAV
CCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCCCEEEEEEECCEEE
SVGSACSSGQLKQSYVLTAMKIPAHEADCAIRISLGWHNTEEEIKQFYTLWQKQYLYHIN
EECCCCCCCCCCHHEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHEEEEEC
ASNIKKQA
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA