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The map label for this gene is spl1 [H]

Identifier: 189183480

GI number: 189183480

Start: 584981

End: 586252

Strand: Reverse

Name: spl1 [H]

Synonym: OTT_0573

Alternate gene names: 189183480

Gene position: 586252-584981 (Counterclockwise)

Preceding gene: 189183481

Following gene: 189183479

Centisome position: 29.18

GC content: 32.94

Gene sequence:

>1272_bases
ATGATAAACACAGTAACAAAAACAGCAGAGTTAAGGTCAAAATTAAAAAACAATAATGTTAAGTTTCCTATTTATATGGA
CTATCAAGCAACTACACCTATTGACACTCGAGTAGTTACTGCAATGTTGCCATATTTAACTGATAAATTTGGCAATCCAC
ATTCACGTAGTCACTTATTTGGTTGGGAATCAGAAGAAGCAGTGGAAGCAGCACGTAAACATGTTGCAGACTTAATTAAA
TCAGATTCAGGTGAAATTATTTTTACTTCTGGAGCAACAGAAGCAAATAATTTAGCCATTAAAGGAGTAGTAGATTTTTA
TAGTAATGACAAAAAAAATCATATAATCACTGCCATGACTGAACATAAATGTGTTATAAATACTTGCAGGCATCTAGAAC
AAAAAGGTATTAAGGTCACTTACTTACCTGTTAATAGTAATGGATTAATTGATATAAATAATCTTAAGAATGCAATCAGT
GACAGTACTATTATGGTATCAATTTCAACAGTTAATAATGAAATTGGGGTTATTCAACCAATCGAGGCCATAGGCCAAAT
TTGTCGTGAGAGAAAAGTTTTTTTTCATACAGATATTGCTCAAGCTTTTGGTAAAATACCAATTGATGTTACTAAATATA
ATATTGACCTTGCCAGCATTTCTGCTCATAAAATTTATGGTCCAAAAGGTATAGGTGCTCTCTATGTTAGAAAAAGGCCA
AGAATACGTATTAGCCCAATTATTCATGGCGGAGGGCAAGAAAGAGGAATGCGATCAGGTACTGTACCTACTCCTTTAGT
AGTGGGATTTGGAGAAGCAGCAAGAATTGCAAATATTGAAATGTTTGAAGAATATAATCGTATTCAAAAAATGTTTAGTA
AGTTTGTTAACTATATATTGAACCATATACCACAAACTTATTTAAATGGAGATATTAAAAGTAGATATCCAGGCAATATT
AACATCAGCTTTGCATGCGTAGAAGGAGAATCTTTAATATTAGCACTCAAGGATCTTGCAATTTCTTCTGGATCTGCTTG
CACTTCTGCTTCATTAGAGCCTTCTTATGTTTTAAAGGCAATGGGAGTTGATGAAGATTTAGCTCATACTTCCTTAAGAT
TTGGCCTTGGTAGATTTACTACTGAAGAAGAAGTGGATTATGCAGTATCACTGATTGTTGATCAAGTAAATCAACTTCGG
GATATTAGCCCACTTTGGGAAATGACACAAAAAGGAATAGATATAAAACAAATTAATTGGTCAGTGCATTAA

Upstream 100 bases:

>100_bases
AAGAAGAAATAAAACAATTTTATACATTATGGCAAAAACAATATTTATATCATATCAATGCTAGTAATATAAAGAAACAA
GCTTAAATCAAATAAATATT

Downstream 100 bases:

>100_bases
GCAACTAATTAACTACAATCGATAAGAGGAAAAAATATTATGAATAATGATGAAAAAACAGTTACAGGTACTCGTACAGC
TGGTTATAGTTCAGAAGTTA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MINTVTKTAELRSKLKNNNVKFPIYMDYQATTPIDTRVVTAMLPYLTDKFGNPHSRSHLFGWESEEAVEAARKHVADLIK
SDSGEIIFTSGATEANNLAIKGVVDFYSNDKKNHIITAMTEHKCVINTCRHLEQKGIKVTYLPVNSNGLIDINNLKNAIS
DSTIMVSISTVNNEIGVIQPIEAIGQICRERKVFFHTDIAQAFGKIPIDVTKYNIDLASISAHKIYGPKGIGALYVRKRP
RIRISPIIHGGGQERGMRSGTVPTPLVVGFGEAARIANIEMFEEYNRIQKMFSKFVNYILNHIPQTYLNGDIKSRYPGNI
NISFACVEGESLILALKDLAISSGSACTSASLEPSYVLKAMGVDEDLAHTSLRFGLGRFTTEEEVDYAVSLIVDQVNQLR
DISPLWEMTQKGIDIKQINWSVH

Sequences:

>Translated_423_residues
MINTVTKTAELRSKLKNNNVKFPIYMDYQATTPIDTRVVTAMLPYLTDKFGNPHSRSHLFGWESEEAVEAARKHVADLIK
SDSGEIIFTSGATEANNLAIKGVVDFYSNDKKNHIITAMTEHKCVINTCRHLEQKGIKVTYLPVNSNGLIDINNLKNAIS
DSTIMVSISTVNNEIGVIQPIEAIGQICRERKVFFHTDIAQAFGKIPIDVTKYNIDLASISAHKIYGPKGIGALYVRKRP
RIRISPIIHGGGQERGMRSGTVPTPLVVGFGEAARIANIEMFEEYNRIQKMFSKFVNYILNHIPQTYLNGDIKSRYPGNI
NISFACVEGESLILALKDLAISSGSACTSASLEPSYVLKAMGVDEDLAHTSLRFGLGRFTTEEEVDYAVSLIVDQVNQLR
DISPLWEMTQKGIDIKQINWSVH
>Mature_423_residues
MINTVTKTAELRSKLKNNNVKFPIYMDYQATTPIDTRVVTAMLPYLTDKFGNPHSRSHLFGWESEEAVEAARKHVADLIK
SDSGEIIFTSGATEANNLAIKGVVDFYSNDKKNHIITAMTEHKCVINTCRHLEQKGIKVTYLPVNSNGLIDINNLKNAIS
DSTIMVSISTVNNEIGVIQPIEAIGQICRERKVFFHTDIAQAFGKIPIDVTKYNIDLASISAHKIYGPKGIGALYVRKRP
RIRISPIIHGGGQERGMRSGTVPTPLVVGFGEAARIANIEMFEEYNRIQKMFSKFVNYILNHIPQTYLNGDIKSRYPGNI
NISFACVEGESLILALKDLAISSGSACTSASLEPSYVLKAMGVDEDLAHTSLRFGLGRFTTEEEVDYAVSLIVDQVNQLR
DISPLWEMTQKGIDIKQINWSVH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=401, Percent_Identity=63.0922693266833, Blast_Score=551, Evalue=1e-157,
Organism=Homo sapiens, GI156713448, Length=428, Percent_Identity=33.6448598130841, Blast_Score=211, Evalue=7e-55,
Organism=Escherichia coli, GI48994898, Length=406, Percent_Identity=57.1428571428571, Blast_Score=502, Evalue=1e-143,
Organism=Escherichia coli, GI1787970, Length=229, Percent_Identity=26.6375545851528, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789175, Length=396, Percent_Identity=23.989898989899, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=401, Percent_Identity=61.3466334164589, Blast_Score=545, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI17533177, Length=328, Percent_Identity=33.2317073170732, Blast_Score=165, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=59.3516209476309, Blast_Score=504, Evalue=1e-144,
Organism=Drosophila melanogaster, GI20129463, Length=430, Percent_Identity=59.0697674418605, Blast_Score=545, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 47022; Mature: 47022

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINTVTKTAELRSKLKNNNVKFPIYMDYQATTPIDTRVVTAMLPYLTDKFGNPHSRSHLF
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
GWESEEAVEAARKHVADLIKSDSGEIIFTSGATEANNLAIKGVVDFYSNDKKNHIITAMT
CCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEEEHHCCCCCCEEEEEEE
EHKCVINTCRHLEQKGIKVTYLPVNSNGLIDINNLKNAISDSTIMVSISTVNNEIGVIQP
CCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEHHHHHHCCCCEEEEEEEECCCCCCCCCH
IEAIGQICRERKVFFHTDIAQAFGKIPIDVTKYNIDLASISAHKIYGPKGIGALYVRKRP
HHHHHHHHHHCCEEEEEHHHHHHCCCCEEEEEEEEEEEECCEEEEECCCCCCEEEEECCC
RIRISPIIHGGGQERGMRSGTVPTPLVVGFGEAARIANIEMFEEYNRIQKMFSKFVNYIL
CEEEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHEEHHHHHHHHHHHHHHHHHHHHHHH
NHIPQTYLNGDIKSRYPGNINISFACVEGESLILALKDLAISSGSACTSASLEPSYVLKA
HHCCHHHHCCCCCCCCCCCEEEEEEEECCCEEEEEEEHHHCCCCCCCCCCCCCHHHHHEE
MGVDEDLAHTSLRFGLGRFTTEEEVDYAVSLIVDQVNQLRDISPLWEMTQKGIDIKQINW
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEE
SVH
ECC
>Mature Secondary Structure
MINTVTKTAELRSKLKNNNVKFPIYMDYQATTPIDTRVVTAMLPYLTDKFGNPHSRSHLF
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
GWESEEAVEAARKHVADLIKSDSGEIIFTSGATEANNLAIKGVVDFYSNDKKNHIITAMT
CCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEEEHHCCCCCCEEEEEEE
EHKCVINTCRHLEQKGIKVTYLPVNSNGLIDINNLKNAISDSTIMVSISTVNNEIGVIQP
CCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEHHHHHHCCCCEEEEEEEECCCCCCCCCH
IEAIGQICRERKVFFHTDIAQAFGKIPIDVTKYNIDLASISAHKIYGPKGIGALYVRKRP
HHHHHHHHHHCCEEEEEHHHHHHCCCCEEEEEEEEEEEECCEEEEECCCCCCEEEEECCC
RIRISPIIHGGGQERGMRSGTVPTPLVVGFGEAARIANIEMFEEYNRIQKMFSKFVNYIL
CEEEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHEEHHHHHHHHHHHHHHHHHHHHHHH
NHIPQTYLNGDIKSRYPGNINISFACVEGESLILALKDLAISSGSACTSASLEPSYVLKA
HHCCHHHHCCCCCCCCCCCEEEEEEEECCCEEEEEEEHHHCCCCCCCCCCCCCHHHHHEE
MGVDEDLAHTSLRFGLGRFTTEEEVDYAVSLIVDQVNQLRDISPLWEMTQKGIDIKQINW
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEE
SVH
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA