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The map label for this gene is mjaIIIM [H]

Identifier: 189183253

GI number: 189183253

Start: 355858

End: 356670

Strand: Reverse

Name: mjaIIIM [H]

Synonym: OTT_0346

Alternate gene names: 189183253

Gene position: 356670-355858 (Counterclockwise)

Preceding gene: 189183254

Following gene: 189183250

Centisome position: 17.75

GC content: 31.0

Gene sequence:

>813_bases
GTGTCAATAGCAATTTCTAATGAACCAAAGCCTTTTCTCCACTGGGTTGGAGGCAAAAGGAGAATTGTTAATAAATTAAT
AGAGCATCTTCCTTCAGGGCCATACTATAATTACTATGAACCATTCCTTGGTGGAGGTGCTTTATTTTTTCAAGTTAGGC
ATCTGTTTAAACAATGTTTTTTGTCTGATATTAATCTCGACTTAATTACTAGTTATCACGCTGTAAAAAAGAATCCAAAT
GAAGTCAACAGATTACTGAATCTATATCACAAAAACCATTCTGAAAATTACTACTATAAAATAAGAGACAATTATTATAG
TAATGATCCTAATGATATCACGGCAAAATTTATATATCTTAATAAATATTCCTTTAGGGGAATTTATAGGCTAAACAGAG
ATGGTACATCTGCTCAAACATTTTCTGATAAGCGATATCTTAAGCTTCATATTGACTCTAGAATAAATAAATGCAGCAAA
CTTTTAACTGGTACATCAATTTGTGCAATGGATTTTTCGTTTATAGAACCTAAAAAAGGTGACTTCGTTTATCTTGATCC
ACCTTACCACCAATCAGGAGAAAGGTTCTACACTAGAGTTCCATTTGATGAAAAAGATCAAATTAGATTACGGGATTTTG
TCCAAGAATTAACAAATAAAGGTGTTAAGATTATGATCTCAAATAATAATACTGCCTTTATTAGAGACTTATACAAAGAT
TTTAATATCAATACCGTTACAGTCGTATACTCAATCAATGAACAACGCAATCCTGTTAATGAGCTAATCATTACTAACTA
TAAAACTTGCTAG

Upstream 100 bases:

>100_bases
AAAGATTTGCAAAAAAGCTCTGTAGCAGTTATTATTACTGGCTTTAAGGCTTGTTTTCTGCTATAATTCATAGTAGAGAT
TTAATTAATCAAAAATTCTT

Downstream 100 bases:

>100_bases
TTATTAAATGTGAACCCGCAATCCAAATCGTATATGAATAGGAAGTGGTCTATAGTTAGCCATTGTGCTCCATTCAATGT
CTAAATATGTATCTCTTGAT

Product: N6 adenine-specific DNA-methyltransferase

Products: NA

Alternate protein names: M.MjaIII; Adenine-specific methyltransferase MjaIII [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MSIAISNEPKPFLHWVGGKRRIVNKLIEHLPSGPYYNYYEPFLGGGALFFQVRHLFKQCFLSDINLDLITSYHAVKKNPN
EVNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYLNKYSFRGIYRLNRDGTSAQTFSDKRYLKLHIDSRINKCSK
LLTGTSICAMDFSFIEPKKGDFVYLDPPYHQSGERFYTRVPFDEKDQIRLRDFVQELTNKGVKIMISNNNTAFIRDLYKD
FNINTVTVVYSINEQRNPVNELIITNYKTC

Sequences:

>Translated_270_residues
MSIAISNEPKPFLHWVGGKRRIVNKLIEHLPSGPYYNYYEPFLGGGALFFQVRHLFKQCFLSDINLDLITSYHAVKKNPN
EVNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYLNKYSFRGIYRLNRDGTSAQTFSDKRYLKLHIDSRINKCSK
LLTGTSICAMDFSFIEPKKGDFVYLDPPYHQSGERFYTRVPFDEKDQIRLRDFVQELTNKGVKIMISNNNTAFIRDLYKD
FNINTVTVVYSINEQRNPVNELIITNYKTC
>Mature_269_residues
SIAISNEPKPFLHWVGGKRRIVNKLIEHLPSGPYYNYYEPFLGGGALFFQVRHLFKQCFLSDINLDLITSYHAVKKNPNE
VNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYLNKYSFRGIYRLNRDGTSAQTFSDKRYLKLHIDSRINKCSKL
LTGTSICAMDFSFIEPKKGDFVYLDPPYHQSGERFYTRVPFDEKDQIRLRDFVQELTNKGVKIMISNNNTAFIRDLYKDF
NINTVTVVYSINEQRNPVNELIITNYKTC

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MjaIII endonuclease (Potential) [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=268, Percent_Identity=29.8507462686567, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31876; Mature: 31744

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIAISNEPKPFLHWVGGKRRIVNKLIEHLPSGPYYNYYEPFLGGGALFFQVRHLFKQCF
CEEEECCCCCHHEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHH
LSDINLDLITSYHAVKKNPNEVNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYL
HCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEEE
NKYSFRGIYRLNRDGTSAQTFSDKRYLKLHIDSRINKCSKLLTGTSICAMDFSFIEPKKG
EEECCCEEEEECCCCCCCCCCCCCEEEEEEECHHHHHHHHHHCCCEEEEECCHHCCCCCC
DFVYLDPPYHQSGERFYTRVPFDEKDQIRLRDFVQELTNKGVKIMISNNNTAFIRDLYKD
CEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
FNINTVTVVYSINEQRNPVNELIITNYKTC
CCCCEEEEEEEECCCCCCHHHEEEECCCCC
>Mature Secondary Structure 
SIAISNEPKPFLHWVGGKRRIVNKLIEHLPSGPYYNYYEPFLGGGALFFQVRHLFKQCF
EEEECCCCCHHEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHH
LSDINLDLITSYHAVKKNPNEVNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYL
HCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEEE
NKYSFRGIYRLNRDGTSAQTFSDKRYLKLHIDSRINKCSKLLTGTSICAMDFSFIEPKKG
EEECCCEEEEECCCCCCCCCCCCCEEEEEEECHHHHHHHHHHCCCEEEEECCHHCCCCCC
DFVYLDPPYHQSGERFYTRVPFDEKDQIRLRDFVQELTNKGVKIMISNNNTAFIRDLYKD
CEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
FNINTVTVVYSINEQRNPVNELIITNYKTC
CCCCEEEEEEEECCCCCCHHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]