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The map label for this gene is traA [H]

Identifier: 189183241

GI number: 189183241

Start: 345696

End: 347006

Strand: Direct

Name: traA [H]

Synonym: OTT_0334

Alternate gene names: 189183241

Gene position: 345696-347006 (Clockwise)

Preceding gene: 189183238

Following gene: 189183242

Centisome position: 17.21

GC content: 33.33

Gene sequence:

>1311_bases
ATGGCTATACAGTTTACAAGGATTGAATTTTTAAGTAGAAGTAAAGGAGGTGATAGTTGTCGTAAGGCAGCGTATAATGC
AAGAACTATTGTTGAAAACGAGAAGACAGGTATAAAGTATAACTTCTCTCGTAAGAAAGATAACGTATATCATACAGTGC
TGATACCGGATTATGTAAAACAAGAATTCAAGAATATTCAAACATTAATGAATGAGGTAGAACGAACAGCCAAAAAAGAC
AACAGCCAGTTGTTGAAGGATATAGTAATAGCATTGCCAGACGAGAAGGAGCTAAATTTAGAGCATAGAATAGAACTAAC
TCATCAAATAGTTGATGAAATGGAATGGGTGCAAAAAGGTATTGGAGTACAGATAGACATTCATAAGCCTCAAACAGGAG
ATAAAAACTGGCATGTACATATATTGCTTACTATGAGAAGATTTAGAGAAGATGGAACTGGTTTAGGAGATATAGCAGTA
GATTTAAACCCAAAAATCATAACATTAAGTAATGGCAAAAAGGTTGTTATTAAAGATCCCGAGATGATTCATGAAAGAGT
AAAAGAAATAATTAATGCATTTTTCGCTAAATTAGGCTTACCATATAGAGTTAAGGATACCAGTAAAGTGCCGGGAAAGC
ATATTGGACCTCGTAGAATTAGGAATTTAATTAATGAAGTATTAAATGAAAATGAGTTACGTAAAGAGGCTCATTTGAAG
ATTATTAATGATGCTGACGTAATAACAGATTCTATAACACATTACAAATCTATTTTTACTAAGCAGGATGTTGAAAAAGC
AGTAAAAGATATACCAGATCTAACAGCAAGAGAACAGTTAGTTCAGCAAGTGCTTAGTTCAAATAGAATACTAGAGTTAT
ACCATGATGATGGTGAAAGTAGCAAATATTTTACGACAATTGAGGTTCGAAATGAGGAGACGAGAATAATCAGAATAGCT
AATAAAATCAATAATCAGGTTTATTACAACGATATTTACAATCTTAAAAGTGATATCGAAGGTCTAGCAAATGTTAGTGA
GGAACAGAAACAAGCGCTAAGGCATATTTTGCTTAGCACTAGTGGAGTTAGAGTCTTGAGAGGAAGAGCTGGTACAGGAA
AATCTTATGTTTTAATAAAAGCGCATAAGCTCGCAACAAATCGTGGACAAAAAGTTATTGGTCTTGCTCCTACTCATAAG
GCAGTATCAGAGCTGAGGAGCAAAGGTTATACAGAGGTCTATACAGTAAAAGGATTTTTATATAATCGAAAAAAAAATTT
TTATGCAAGACAGCTTAATAGTAGTAGATGA

Upstream 100 bases:

>100_bases
GTACTCATTAACAGATTGAATGTTAAAAAAAAAGAAGTTTGTGCTAGCAAAATCAAAAAAACCATGCTAACTTAAAACTC
AGGTAAGAATTGGTATTGAG

Downstream 100 bases:

>100_bases
AGCTGGAATGGTAGGTACTAAAGCTTATGCAGAGCTGTTTAGAGTAGTTAGAAACAATAATTGTCAACTGATACTTGCTG
GAGATGAAAAGCAGTTAGCT

Product: putative conjugative transfer protein TraA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MAIQFTRIEFLSRSKGGDSCRKAAYNARTIVENEKTGIKYNFSRKKDNVYHTVLIPDYVKQEFKNIQTLMNEVERTAKKD
NSQLLKDIVIALPDEKELNLEHRIELTHQIVDEMEWVQKGIGVQIDIHKPQTGDKNWHVHILLTMRRFREDGTGLGDIAV
DLNPKIITLSNGKKVVIKDPEMIHERVKEIINAFFAKLGLPYRVKDTSKVPGKHIGPRRIRNLINEVLNENELRKEAHLK
IINDADVITDSITHYKSIFTKQDVEKAVKDIPDLTAREQLVQQVLSSNRILELYHDDGESSKYFTTIEVRNEETRIIRIA
NKINNQVYYNDIYNLKSDIEGLANVSEEQKQALRHILLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRGQKVIGLAPTHK
AVSELRSKGYTEVYTVKGFLYNRKKNFYARQLNSSR

Sequences:

>Translated_436_residues
MAIQFTRIEFLSRSKGGDSCRKAAYNARTIVENEKTGIKYNFSRKKDNVYHTVLIPDYVKQEFKNIQTLMNEVERTAKKD
NSQLLKDIVIALPDEKELNLEHRIELTHQIVDEMEWVQKGIGVQIDIHKPQTGDKNWHVHILLTMRRFREDGTGLGDIAV
DLNPKIITLSNGKKVVIKDPEMIHERVKEIINAFFAKLGLPYRVKDTSKVPGKHIGPRRIRNLINEVLNENELRKEAHLK
IINDADVITDSITHYKSIFTKQDVEKAVKDIPDLTAREQLVQQVLSSNRILELYHDDGESSKYFTTIEVRNEETRIIRIA
NKINNQVYYNDIYNLKSDIEGLANVSEEQKQALRHILLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRGQKVIGLAPTHK
AVSELRSKGYTEVYTVKGFLYNRKKNFYARQLNSSR
>Mature_435_residues
AIQFTRIEFLSRSKGGDSCRKAAYNARTIVENEKTGIKYNFSRKKDNVYHTVLIPDYVKQEFKNIQTLMNEVERTAKKDN
SQLLKDIVIALPDEKELNLEHRIELTHQIVDEMEWVQKGIGVQIDIHKPQTGDKNWHVHILLTMRRFREDGTGLGDIAVD
LNPKIITLSNGKKVVIKDPEMIHERVKEIINAFFAKLGLPYRVKDTSKVPGKHIGPRRIRNLINEVLNENELRKEAHLKI
INDADVITDSITHYKSIFTKQDVEKAVKDIPDLTAREQLVQQVLSSNRILELYHDDGESSKYFTTIEVRNEETRIIRIAN
KINNQVYYNDIYNLKSDIEGLANVSEEQKQALRHILLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRGQKVIGLAPTHKA
VSELRSKGYTEVYTVKGFLYNRKKNFYARQLNSSR

Specific function: traI Has Been Identified As DNA Helicase I And It Also Has An Additional Activity Of Site-Specific Nicking At Orit. DNA Helicase I Is A Potent DNA-Dependent ATPase. [C]

COG id: COG0507

COG function: function code L; ATP-dependent exoDNAse (exonuclease V), alpha subunit - helicase superfamily I member

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mobA/mobL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000606
- InterPro:   IPR005053
- InterPro:   IPR014136 [H]

Pfam domain/function: PF03389 MobA_MobL; PF01443 Viral_helicase1 [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 50167; Mature: 50036

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIQFTRIEFLSRSKGGDSCRKAAYNARTIVENEKTGIKYNFSRKKDNVYHTVLIPDYVK
CCEEEHHHHHHHCCCCCCHHHHHHCCCHHEEECCCCCEEEEECCCCCCEEEEEECCHHHH
QEFKNIQTLMNEVERTAKKDNSQLLKDIVIALPDEKELNLEHRIELTHQIVDEMEWVQKG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
IGVQIDIHKPQTGDKNWHVHILLTMRRFREDGTGLGDIAVDLNPKIITLSNGKKVVIKDP
CCEEEEEECCCCCCCCEEEEEEEEHHHHHHCCCCCCEEEEECCCEEEEECCCCEEEEECH
EMIHERVKEIINAFFAKLGLPYRVKDTSKVPGKHIGPRRIRNLINEVLNENELRKEAHLK
HHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCEE
IINDADVITDSITHYKSIFTKQDVEKAVKDIPDLTAREQLVQQVLSSNRILELYHDDGES
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
SKYFTTIEVRNEETRIIRIANKINNQVYYNDIYNLKSDIEGLANVSEEQKQALRHILLST
CEEEEEEEEECCCCEEEEEEHHHCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
SGVRVLRGRAGTGKSYVLIKAHKLATNRGQKVIGLAPTHKAVSELRSKGYTEVYTVKGFL
CCCEEEECCCCCCCEEEEEEEEEHHCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEEEE
YNRKKNFYARQLNSSR
EECCCCHHHHHCCCCC
>Mature Secondary Structure 
AIQFTRIEFLSRSKGGDSCRKAAYNARTIVENEKTGIKYNFSRKKDNVYHTVLIPDYVK
CEEEHHHHHHHCCCCCCHHHHHHCCCHHEEECCCCCEEEEECCCCCCEEEEEECCHHHH
QEFKNIQTLMNEVERTAKKDNSQLLKDIVIALPDEKELNLEHRIELTHQIVDEMEWVQKG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
IGVQIDIHKPQTGDKNWHVHILLTMRRFREDGTGLGDIAVDLNPKIITLSNGKKVVIKDP
CCEEEEEECCCCCCCCEEEEEEEEHHHHHHCCCCCCEEEEECCCEEEEECCCCEEEEECH
EMIHERVKEIINAFFAKLGLPYRVKDTSKVPGKHIGPRRIRNLINEVLNENELRKEAHLK
HHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCEE
IINDADVITDSITHYKSIFTKQDVEKAVKDIPDLTAREQLVQQVLSSNRILELYHDDGES
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
SKYFTTIEVRNEETRIIRIANKINNQVYYNDIYNLKSDIEGLANVSEEQKQALRHILLST
CEEEEEEEEECCCCEEEEEEHHHCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
SGVRVLRGRAGTGKSYVLIKAHKLATNRGQKVIGLAPTHKAVSELRSKGYTEVYTVKGFL
CCCEEEECCCCCCCEEEEEEEEEHHCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEEEE
YNRKKNFYARQLNSSR
EECCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on acid anhydrides; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8763953; 11743193; 11743194 [H]