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The map label for this gene is dnaB [H]

Identifier: 189183221

GI number: 189183221

Start: 326647

End: 328083

Strand: Direct

Name: dnaB [H]

Synonym: OTT_0314

Alternate gene names: 189183221

Gene position: 326647-328083 (Clockwise)

Preceding gene: 189183220

Following gene: 189183222

Centisome position: 16.26

GC content: 31.94

Gene sequence:

>1437_bases
ATGGAAAAAGATCTTGCAAAGATTGCTCCAAGTAATATTCAGGCAGAGCAAATGATACTTGGAGCAATTCTGATTAACAA
TCGTGCGCTATATAACATTAACGAATTTTTACTGCCGGAACATTTTTATGAACCATTACATGGTAAAATATACAAGTCAA
TTAATCTCATTATTAGTAAAGGAATTAGTGCTACTGTAATTTCGCTCAAAAATATGCTAGGCAATGAACCCTCATTTGAG
GAAATAGGTGGAGTAGATTATCTAGCTAAACTTACAACTTTAGCATTAAGTATAGTTAATGTTAATGAGTACGGCAAAAT
AGTATATGATCTTGCGCTGAGGCGTTATTTAATTGAAATTGGAGAAAAAATAGTAACAAATGCATATTCTTCTACTTTAG
CAGATACAGCTATAAGTCAGATCAAAACTGCTGAATCTCAATTATATGATCTAGGTGCAAGAGGAACTTTAAGTAAAGGA
TTTACAAAATTACAAACTTCAATTGAAGAATCATGGACATCAATTTCATCTGCTATTAAAAATAAAAACTCTATTAACGG
TATTAGTAGTGGACTACTTGACCTTGATTCAAAGCTTGGAGGATTTAAAAATTCTGACCTAATAATATTAGCTGGCAGGC
CATCAATGGGTAAAACTGCTTTAGGAGTTAACTTAGCAATAAATGCTTGTAAATATTTTCTTACTCAAAAAAATACTAAA
GATAATGTAGTACTATCAGTTGGCTTCTTTTCTTTAGAAATGTCATCTCAGCAAATCTCTACTCGAATTCTTTCTATAGA
ATCAGAAATTAATAGCTCTGCATTATTTAACGGTAAAATAGATGAACAAGATGTTGATAAGTTGAAGACTGTACAAGATG
AAATACAAAAGTGGAATTTTTTTATAGATGACGCTCCAGCAATCTCGATATCTGCAATTAGATCTCGAGCTCGTAGACTT
AAACGTACTCATAATTTAGCAATATTATTTATTGATTATTTACAGCTAATAAAAATTGATAACAGAGGAAGTCAGTATAA
TCGAGTACAGGAGATTTCTGAAATTACTCAGAGCTTAAAAGCTCTTGCTAAAGATCTCAATATTTCAATCATTGCATTAT
CTCAATTATCTAGAGCTGTAGAACAAAGGTCAGATAAAAAGCCTCTACTATCAGATCTACGAGAATCAGGCTCCATTGAA
CAAGATGCCGATATTGTAATGCTTATATATCGTGACGAATATTACTTGTCTAGATCAGAACCGAATCCAGGTACTCCAGA
ATATACAGAATGGGTTACAAAACAAAATAAATGTTATAACACTGCTGAAATAATTGTTGCTAAACACCGTAATGGGCCAG
TTGGTACAGTGAAGTTGCACTATAATAGTAGGTATTCTAAATTTGGCAATATTGTTAAAAACTATCAGCAAGCTTAA

Upstream 100 bases:

>100_bases
GAGAGAACTATCTGAAAGATCTTGAGCCCGCTTTTAAAGCTCAAGGATTTTCTTTCGAGTTAGTTAAGTTTAGTAATTTT
AATAAAATTTAAAGGGTGAT

Downstream 100 bases:

>100_bases
TAAACACTAGATTATGCATAAATGAAAAAATGCTGGTCCAAAAAAAAAATTACAGCAAAATTTTACTCATTAACACCAGA
GATATGCAACTTAAGGTTAA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNEPSFE
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGEKIVTNAYSSTLADTAISQIKTAESQLYDLGARGTLSKG
FTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLTQKNTK
DNVVLSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQDEIQKWNFFIDDAPAISISAIRSRARRL
KRTHNLAILFIDYLQLIKIDNRGSQYNRVQEISEITQSLKALAKDLNISIIALSQLSRAVEQRSDKKPLLSDLRESGSIE
QDADIVMLIYRDEYYLSRSEPNPGTPEYTEWVTKQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIVKNYQQA

Sequences:

>Translated_478_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNEPSFE
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGEKIVTNAYSSTLADTAISQIKTAESQLYDLGARGTLSKG
FTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLTQKNTK
DNVVLSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQDEIQKWNFFIDDAPAISISAIRSRARRL
KRTHNLAILFIDYLQLIKIDNRGSQYNRVQEISEITQSLKALAKDLNISIIALSQLSRAVEQRSDKKPLLSDLRESGSIE
QDADIVMLIYRDEYYLSRSEPNPGTPEYTEWVTKQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIVKNYQQA
>Mature_478_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNEPSFE
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGEKIVTNAYSSTLADTAISQIKTAESQLYDLGARGTLSKG
FTKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLTQKNTK
DNVVLSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQDEIQKWNFFIDDAPAISISAIRSRARRL
KRTHNLAILFIDYLQLIKIDNRGSQYNRVQEISEITQSLKALAKDLNISIIALSQLSRAVEQRSDKKPLLSDLRESGSIE
QDADIVMLIYRDEYYLSRSEPNPGTPEYTEWVTKQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIVKNYQQA

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=468, Percent_Identity=35.4700854700855, Blast_Score=288, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 53373; Mature: 53373

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHCCCCCCCHHHHHHHHHEECCCEEECCHHHHCCHHHHCCHHHHHHHHHHHHHHC
GISATVISLKNMLGNEPSFEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEI
CCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHH
GEKIVTNAYSSTLADTAISQIKTAESQLYDLGARGTLSKGFTKLQTSIEESWTSISSAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLTQKNTK
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHCCCCC
DNVVLSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQDEIQKWNF
CCEEEEEEEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCE
FIDDAPAISISAIRSRARRLKRTHNLAILFIDYLQLIKIDNRGSQYNRVQEISEITQSLK
EECCCCCHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHH
ALAKDLNISIIALSQLSRAVEQRSDKKPLLSDLRESGSIEQDADIVMLIYRDEYYLSRSE
HHHHHCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCEEEECCC
PNPGTPEYTEWVTKQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIVKNYQQA
CCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHCCCCCCCHHHHHHHHHEECCCEEECCHHHHCCHHHHCCHHHHHHHHHHHHHHC
GISATVISLKNMLGNEPSFEEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEI
CCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHH
GEKIVTNAYSSTLADTAISQIKTAESQLYDLGARGTLSKGFTKLQTSIEESWTSISSAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAINACKYFLTQKNTK
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHCCCCC
DNVVLSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIDEQDVDKLKTVQDEIQKWNF
CCEEEEEEEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCE
FIDDAPAISISAIRSRARRLKRTHNLAILFIDYLQLIKIDNRGSQYNRVQEISEITQSLK
EECCCCCHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHH
ALAKDLNISIIALSQLSRAVEQRSDKKPLLSDLRESGSIEQDADIVMLIYRDEYYLSRSE
HHHHHCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCEEEECCC
PNPGTPEYTEWVTKQNKCYNTAEIIVAKHRNGPVGTVKLHYNSRYSKFGNIVKNYQQA
CCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA