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The map label for this gene is radA [H]

Identifier: 189182977

GI number: 189182977

Start: 73756

End: 75111

Strand: Reverse

Name: radA [H]

Synonym: OTT_0070

Alternate gene names: 189182977

Gene position: 75111-73756 (Counterclockwise)

Preceding gene: 189182978

Following gene: 189182975

Centisome position: 3.74

GC content: 32.01

Gene sequence:

>1356_bases
ATGAAAACAAAGAAACAATATACTTGCACTAATTGTGGTAATATAAGCTATAAGTGGTATGGCAGATGCTCTGAATGTAA
TGAATGGAATAGTATTATAGAAGATGTTATATCTAATAACTCTTATAATAGTAGCACCAGTATTACTGGTAATAATTTAA
CTATTAGTTCTCTTGATAAAGATATTGATGAACCATGTAGAATTGTCAGTTCAATTAATGAACTAAATAGAGTACTTGGA
GGAGGATTAATTCCAGAATCAGCAATTCTTATAGGTGGTGATCCAGGCATTGGAAAATCGACCTTATTACTTCAGTTATG
TGCTCAATTATCTACTTCATCTACTTCTTGTTTTTATATTTCTGGCGAAGAATCAATTAATCAGATCAAGCTTAGAGCTC
AAAGATTAGGTTTGGTAAACACGCAAATTAAACTTGTTTCTGCTACTAATATTCATGATATCATAGCTACCATTAAAGCA
AATAAGCAAGAAATCCATCTAGTTGTTATTGATTCTATACAAACAATTTATAATCCTGAAATTACGTCCATAGCTGGCAC
AACATCGCAAGTTCGAGCAGCAGCACAAATGTTAATATCTTTAACTAAATTAATTGGTATAGCACTAATTATGGTAGGTC
ATATCACTAAAGATGGACAGCTAGCTGGGCCTAAAATTCTTGAGCATATGGTCGACACTGTTTTGTATCTTGAGGGCAGT
TCAAATTATCAATATAGACTTTTACGATCGATAAAAAATCGATTTGGTAAAACAAATGAAATAGGTGTTTTTACTATGGA
ACAATCTGGATTAATAGAAGTATCAAATCCATCAGAAATGTTTATACTCAATACTGAAACAAATTATAGCGGCACTAGTG
TTTTTGCGAGTATGGAAGGATCACGTCCTATTCTAGTAGAGATTCAAGCTTTAATATCACCTTCACATATTCCAACTCCA
AGGCGTTCTGTAGTTGGATGGGATAGTAACAGATTATCAATGATACTAGCTGTATTAGCTGTACGATATGGATTAACTCT
TACATCTTATGAAGTATATTTAAGCGTAGCAGGCGGATTAAAAATTACAGAACCAGCTGTTGATTTAGCTGTTGCTGCTT
CCTTGATTTCTGCAGCGTCAAACCAACCATTACCACTACACAGTATTTTTTTTGGAGAAATTAGCTTATCTGGAGAAATT
AGAAAATCTACTCAATCAGACCTTAGAGTTAATGAAGCTGTAAAGCTTGGTTTTAAAAATATTTTTTGTGCTGATTATAG
TAATAATTCATCAACTAATTCAGATTATAACATATATAACATTAAACATATTAAGCAGCTAAAAAATATCTTATGA

Upstream 100 bases:

>100_bases
CAGAGTTAAATCATAAGTTAGTATTACCAGTCAATTTTCATTTTCAGGCAGAAAAAATTTATGGTAAAAGTAAGTTAACA
ATTTTACTTTATAAAAATTA

Downstream 100 bases:

>100_bases
CCAGCTAACATAGAATACTAATATTATAACAGCTATCTCATAAATCTTTAGCTTTAGAATTATTTGGGGCCACGTTAATT
AAATAAGCAGAGAATATAAT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MKTKKQYTCTNCGNISYKWYGRCSECNEWNSIIEDVISNNSYNSSTSITGNNLTISSLDKDIDEPCRIVSSINELNRVLG
GGLIPESAILIGGDPGIGKSTLLLQLCAQLSTSSTSCFYISGEESINQIKLRAQRLGLVNTQIKLVSATNIHDIIATIKA
NKQEIHLVVIDSIQTIYNPEITSIAGTTSQVRAAAQMLISLTKLIGIALIMVGHITKDGQLAGPKILEHMVDTVLYLEGS
SNYQYRLLRSIKNRFGKTNEIGVFTMEQSGLIEVSNPSEMFILNTETNYSGTSVFASMEGSRPILVEIQALISPSHIPTP
RRSVVGWDSNRLSMILAVLAVRYGLTLTSYEVYLSVAGGLKITEPAVDLAVAASLISAASNQPLPLHSIFFGEISLSGEI
RKSTQSDLRVNEAVKLGFKNIFCADYSNNSSTNSDYNIYNIKHIKQLKNIL

Sequences:

>Translated_451_residues
MKTKKQYTCTNCGNISYKWYGRCSECNEWNSIIEDVISNNSYNSSTSITGNNLTISSLDKDIDEPCRIVSSINELNRVLG
GGLIPESAILIGGDPGIGKSTLLLQLCAQLSTSSTSCFYISGEESINQIKLRAQRLGLVNTQIKLVSATNIHDIIATIKA
NKQEIHLVVIDSIQTIYNPEITSIAGTTSQVRAAAQMLISLTKLIGIALIMVGHITKDGQLAGPKILEHMVDTVLYLEGS
SNYQYRLLRSIKNRFGKTNEIGVFTMEQSGLIEVSNPSEMFILNTETNYSGTSVFASMEGSRPILVEIQALISPSHIPTP
RRSVVGWDSNRLSMILAVLAVRYGLTLTSYEVYLSVAGGLKITEPAVDLAVAASLISAASNQPLPLHSIFFGEISLSGEI
RKSTQSDLRVNEAVKLGFKNIFCADYSNNSSTNSDYNIYNIKHIKQLKNIL
>Mature_451_residues
MKTKKQYTCTNCGNISYKWYGRCSECNEWNSIIEDVISNNSYNSSTSITGNNLTISSLDKDIDEPCRIVSSINELNRVLG
GGLIPESAILIGGDPGIGKSTLLLQLCAQLSTSSTSCFYISGEESINQIKLRAQRLGLVNTQIKLVSATNIHDIIATIKA
NKQEIHLVVIDSIQTIYNPEITSIAGTTSQVRAAAQMLISLTKLIGIALIMVGHITKDGQLAGPKILEHMVDTVLYLEGS
SNYQYRLLRSIKNRFGKTNEIGVFTMEQSGLIEVSNPSEMFILNTETNYSGTSVFASMEGSRPILVEIQALISPSHIPTP
RRSVVGWDSNRLSMILAVLAVRYGLTLTSYEVYLSVAGGLKITEPAVDLAVAASLISAASNQPLPLHSIFFGEISLSGEI
RKSTQSDLRVNEAVKLGFKNIFCADYSNNSSTNSDYNIYNIKHIKQLKNIL

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=448, Percent_Identity=40.625, Blast_Score=364, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR020588
- InterPro:   IPR004504
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC [H]

EC number: NA

Molecular weight: Translated: 49260; Mature: 49260

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKKQYTCTNCGNISYKWYGRCSECNEWNSIIEDVISNNSYNSSTSITGNNLTISSLDK
CCCCCCEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEECCCCEEEEHHHC
DIDEPCRIVSSINELNRVLGGGLIPESAILIGGDPGIGKSTLLLQLCAQLSTSSTSCFYI
CHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEE
SGEESINQIKLRAQRLGLVNTQIKLVSATNIHDIIATIKANKQEIHLVVIDSIQTIYNPE
ECCCCHHHHHHHHHHCCEEECEEEEEECCCHHHHHHHEECCCCEEEEEEEECHHHHCCCC
ITSIAGTTSQVRAAAQMLISLTKLIGIALIMVGHITKDGQLAGPKILEHMVDTVLYLEGS
HHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEEEEECC
SNYQYRLLRSIKNRFGKTNEIGVFTMEQSGLIEVSNPSEMFILNTETNYSGTSVFASMEG
CCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCEEEEEECCCCCCCCEEEEECCC
SRPILVEIQALISPSHIPTPRRSVVGWDSNRLSMILAVLAVRYGLTLTSYEVYLSVAGGL
CCCEEEEEEEHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCEEEEEEEEEEEECCC
KITEPAVDLAVAASLISAASNQPLPLHSIFFGEISLSGEIRKSTQSDLRVNEAVKLGFKN
EECCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCHHHHCCHHHHHHHHHHHHCCCE
IFCADYSNNSSTNSDYNIYNIKHIKQLKNIL
EEEEECCCCCCCCCCEEEEEHHHHHHHHHCC
>Mature Secondary Structure
MKTKKQYTCTNCGNISYKWYGRCSECNEWNSIIEDVISNNSYNSSTSITGNNLTISSLDK
CCCCCCEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEECCCCEEEEHHHC
DIDEPCRIVSSINELNRVLGGGLIPESAILIGGDPGIGKSTLLLQLCAQLSTSSTSCFYI
CHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEE
SGEESINQIKLRAQRLGLVNTQIKLVSATNIHDIIATIKANKQEIHLVVIDSIQTIYNPE
ECCCCHHHHHHHHHHCCEEECEEEEEECCCHHHHHHHEECCCCEEEEEEEECHHHHCCCC
ITSIAGTTSQVRAAAQMLISLTKLIGIALIMVGHITKDGQLAGPKILEHMVDTVLYLEGS
HHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEEEEECC
SNYQYRLLRSIKNRFGKTNEIGVFTMEQSGLIEVSNPSEMFILNTETNYSGTSVFASMEG
CCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCEEEEEECCCCCCCCEEEEECCC
SRPILVEIQALISPSHIPTPRRSVVGWDSNRLSMILAVLAVRYGLTLTSYEVYLSVAGGL
CCCEEEEEEEHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCEEEEEEEEEEEECCC
KITEPAVDLAVAASLISAASNQPLPLHSIFFGEISLSGEIRKSTQSDLRVNEAVKLGFKN
EECCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCHHHHCCHHHHHHHHHHHHCCCE
IFCADYSNNSSTNSDYNIYNIKHIKQLKNIL
EEEEECCCCCCCCCCEEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA