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The map label for this gene is prfA [H]

Identifier: 189182919

GI number: 189182919

Start: 16647

End: 17726

Strand: Direct

Name: prfA [H]

Synonym: OTT_0012

Alternate gene names: 189182919

Gene position: 16647-17726 (Clockwise)

Preceding gene: 189182918

Following gene: 189182920

Centisome position: 0.83

GC content: 31.94

Gene sequence:

>1080_bases
ATGAGTTTTACTGTTCACTTGAATAAAATTTTACAAAAATATCAAGATCTTGAAGCTGATTTAAATGGTGGTAAGCTGGA
CAATAAAGAGCTAGCTTTAATTTCTAAAGAATATTCTAATCTTAAGCCTATAATTGAAAAACTTAATCGCTATTTAAAAG
CTCATGAAAATATAAAATACTTGCAACAAGTAATTGATACAGAACAGGATTTAGAATTAAAGTCAATAGCAGAAGTTGAA
TTATATGATACCTTAAATTATTTGCCAAAGTTAGAAGAAGAAGTTAAAATATCTCTCCTTCCTAAAGAGAGTGATGATCA
TAAAAATGCAATTATTGAAGTTAGAGCAGGCACTGGCGGAGATGAAGCGGCGTTATTTGCAGCATCTCTATTTCAGATGT
ACAATAGATATGCTGAACGTAAAAAGTGGAAGTTTGAACTTTTATCGATATCAGATACAGAAATTGGTGGATATAAGGAA
GCATCAGCTTTGATTAGTGGAAACGGTGTATTTGCTAATTTAAAATTTGAATCAGGAGTACATAGAGTCCAGAGAATACC
TAAAACAGAATCAAATGGTAGAATACATACTTCTGCTGCAACTGTTGTAGTTCTTCCAGAAGCTGAAGAAGTTGATGTAA
AAATTGATGCTAAAGATCTTAAAATAGATACATACCGCGCATCTGGGGCTGGAGGACAGCATGTTAATACAACTGATTCC
GCAGTAAGAATTACTCATATTCCTACTGGTGTTGTAGTATCTCAACAAGATGAGAAATCACAACATAAAAATAAAGCTAA
GGCAATGAAAATATTGTATGCTAGATTATATGACTTAGAAAAACAAAAAAGCCAGCAAGAGCAAGCAATGTCACGAAAAG
TTCAAGTTGGAACTGGCGATCGTTCAGAACGTATTAGAACTTATAATTATCCTCAAGGTAGAGTAACTGATCATAGAATT
AACCTTACGTTATATAAAATCGAAGAAATTATTCAAGAGGGTAAATTAGACGAAATCATTAATAATTTGATCTCGGAAAA
TGAGGCAAAAAAAATAGCTGATAGTAATATTCAATTTTGA

Upstream 100 bases:

>100_bases
TGAGTCATGATAATAATTCTTGATTAATAGGAAGTATTTGACTGATCTTAATTTTAGAAGTATGACTTTAATAATTTTGA
GCAAATAAGTATCGAAAAGC

Downstream 100 bases:

>100_bases
GAATCCTTTTGTAGCATAGATAAAAGAATTAAATTTGAGTAATGCAAGGGTTAGTTTATAGTATTACTGCATATTGTGGA
AAGATTTTATGCTATTATTT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSFTVHLNKILQKYQDLEADLNGGKLDNKELALISKEYSNLKPIIEKLNRYLKAHENIKYLQQVIDTEQDLELKSIAEVE
LYDTLNYLPKLEEEVKISLLPKESDDHKNAIIEVRAGTGGDEAALFAASLFQMYNRYAERKKWKFELLSISDTEIGGYKE
ASALISGNGVFANLKFESGVHRVQRIPKTESNGRIHTSAATVVVLPEAEEVDVKIDAKDLKIDTYRASGAGGQHVNTTDS
AVRITHIPTGVVVSQQDEKSQHKNKAKAMKILYARLYDLEKQKSQQEQAMSRKVQVGTGDRSERIRTYNYPQGRVTDHRI
NLTLYKIEEIIQEGKLDEIINNLISENEAKKIADSNIQF

Sequences:

>Translated_359_residues
MSFTVHLNKILQKYQDLEADLNGGKLDNKELALISKEYSNLKPIIEKLNRYLKAHENIKYLQQVIDTEQDLELKSIAEVE
LYDTLNYLPKLEEEVKISLLPKESDDHKNAIIEVRAGTGGDEAALFAASLFQMYNRYAERKKWKFELLSISDTEIGGYKE
ASALISGNGVFANLKFESGVHRVQRIPKTESNGRIHTSAATVVVLPEAEEVDVKIDAKDLKIDTYRASGAGGQHVNTTDS
AVRITHIPTGVVVSQQDEKSQHKNKAKAMKILYARLYDLEKQKSQQEQAMSRKVQVGTGDRSERIRTYNYPQGRVTDHRI
NLTLYKIEEIIQEGKLDEIINNLISENEAKKIADSNIQF
>Mature_358_residues
SFTVHLNKILQKYQDLEADLNGGKLDNKELALISKEYSNLKPIIEKLNRYLKAHENIKYLQQVIDTEQDLELKSIAEVEL
YDTLNYLPKLEEEVKISLLPKESDDHKNAIIEVRAGTGGDEAALFAASLFQMYNRYAERKKWKFELLSISDTEIGGYKEA
SALISGNGVFANLKFESGVHRVQRIPKTESNGRIHTSAATVVVLPEAEEVDVKIDAKDLKIDTYRASGAGGQHVNTTDSA
VRITHIPTGVVVSQQDEKSQHKNKAKAMKILYARLYDLEKQKSQQEQAMSRKVQVGTGDRSERIRTYNYPQGRVTDHRIN
LTLYKIEEIIQEGKLDEIINNLISENEAKKIADSNIQF

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=273, Percent_Identity=50.1831501831502, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI34577120, Length=360, Percent_Identity=42.7777777777778, Blast_Score=271, Evalue=7e-73,
Organism=Homo sapiens, GI166795305, Length=178, Percent_Identity=51.123595505618, Blast_Score=197, Evalue=1e-50,
Organism=Escherichia coli, GI1787462, Length=355, Percent_Identity=50.9859154929577, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI2367172, Length=310, Percent_Identity=38.0645161290323, Blast_Score=195, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=305, Percent_Identity=38.3606557377049, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=356, Percent_Identity=42.1348314606742, Blast_Score=245, Evalue=7e-66,
Organism=Drosophila melanogaster, GI19921226, Length=333, Percent_Identity=40.8408408408408, Blast_Score=234, Evalue=8e-62,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40679; Mature: 40547

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTVHLNKILQKYQDLEADLNGGKLDNKELALISKEYSNLKPIIEKLNRYLKAHENIKY
CEEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCHHHH
LQQVIDTEQDLELKSIAEVELYDTLNYLPKLEEEVKISLLPKESDDHKNAIIEVRAGTGG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEECCCCC
DEAALFAASLFQMYNRYAERKKWKFELLSISDTEIGGYKEASALISGNGVFANLKFESGV
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHEEEECCCEEEEEEHHHHH
HRVQRIPKTESNGRIHTSAATVVVLPEAEEVDVKIDAKDLKIDTYRASGAGGQHVNTTDS
HHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCC
AVRITHIPTGVVVSQQDEKSQHKNKAKAMKILYARLYDLEKQKSQQEQAMSRKVQVGTGD
EEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
RSERIRTYNYPQGRVTDHRINLTLYKIEEIIQEGKLDEIINNLISENEAKKIADSNIQF
CCCCCEEECCCCCCEECEEEEEEHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHCCCCCC
>Mature Secondary Structure 
SFTVHLNKILQKYQDLEADLNGGKLDNKELALISKEYSNLKPIIEKLNRYLKAHENIKY
EEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCHHHH
LQQVIDTEQDLELKSIAEVELYDTLNYLPKLEEEVKISLLPKESDDHKNAIIEVRAGTGG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEECCCCC
DEAALFAASLFQMYNRYAERKKWKFELLSISDTEIGGYKEASALISGNGVFANLKFESGV
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHEEEECCCEEEEEEHHHHH
HRVQRIPKTESNGRIHTSAATVVVLPEAEEVDVKIDAKDLKIDTYRASGAGGQHVNTTDS
HHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCC
AVRITHIPTGVVVSQQDEKSQHKNKAKAMKILYARLYDLEKQKSQQEQAMSRKVQVGTGD
EEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
RSERIRTYNYPQGRVTDHRINLTLYKIEEIIQEGKLDEIINNLISENEAKKIADSNIQF
CCCCCEEECCCCCCEECEEEEEEHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA