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The map label for this gene is aspS

Identifier: 189026208

GI number: 189026208

Start: 1070147

End: 1071955

Strand: Direct

Name: aspS

Synonym: TPASS_0985

Alternate gene names: 189026208

Gene position: 1070147-1071955 (Clockwise)

Preceding gene: 189026206

Following gene: 189026209

Centisome position: 93.92

GC content: 54.28

Gene sequence:

>1809_bases
TTGTATAGCGCTCACACCCCATGGTATGATGGGCAGGTCATGGATTATCCGAGAAGGACTATAGCTTGTGGCGAGCTGCG
CAGGTGCCACGTCGGAACGGTAGTTGTGCTCAATGGATGGGTCCACCGAAAGCGGTCGCACGGAACCGTTAGTTTCTTTA
ACATGCGCGATAGGTCCGGAATAGTGCAGGTTATAGTGAGCCAGGAGGAAAACGCTAGCCTGTGGTCCACGGTAAACCGC
ATACGGTTGGAATGCTGTCTTGCAGTCGAAGGCGTGGTGCGAGAGCGACCTCCTTCAATGATAAATCGCGCCCTGCATAC
CGGGGAGGTGGAGGTGCACGCTCGCACGCTGTACGTTCTCTCGGAGAATGCTGTGCTTCCGTTCCGCGTTGATGATGTTG
TGCATGCGCACGAAGATATACGCTTAAAATATCGCTACCTCGACCTGCGCTCTCAGCGCATGCAGGAGCGCATTGCACTG
CGCTCACGCGTTGCCCTGGCCATACGGCAGTTTTTAAGTATGAAAGGTTTCATCGAGATCGAAACTCCCACCTTCATCTG
CTCTACCCCCGAGGGGGCACGTGACTTTGTTGTCCCTTCCCGAGTGTGCCCCGGGCGTTTCTATGCCCTGCCACAGTCCC
CCCAGCTGTACAAGCAGCTTCTGATGGTGGCAGGGTTTGACCGCTATTTCCAACTTGCCCGTTGCTACCGAGACGAGGAT
GCACGAGGCGATCGTCAGCCAGAATTTACCCAGATAGACCTTGAGATGAGCTTCGTTTCTCGAGACGATGTTATGCGGGT
GAACGAGGATATGCTTCGGTACGTGTTTAGAACCAGCATCGGTGTCGAACTGCCTACCTTTTTTCCTCGGCTTACCTACG
CGCAGGCGCTAGACCAATATGGAACAGATAAGCCAGACATGCGCTTCAAACCGGTCCTGCAGAATGCAGACTTTATGGGA
ATGCTTGGCACGTTCACCCCGTTTGAAGAAGTCGTCGCACAGGGTGGCAGCATCAGAGCACTCGTTCTTCCGGGCAAGGC
ACGTTGCTACAGCCGTAGGCAAATCGAAGCGTTGGAGTCTATCGCTCGAGCACATGAGGCGCACCACCTTTTTTGGCTTA
AGGCAACCGGTGGAGGCCTCGAGGGGGGTATCGCAAGGTTTTTTGCAGGGGTAGAGTCCGAAGTACGCCGGCGACTTTCT
GCTCAGGATGAAGACTTGTTGCTCTTTGTCGCCGATTGCCGGCACCGCGTGTGCTGCGTCGCACTCGGCGCAGTGCGCAG
CGCTCTTATCAGGGACGAGTCGTTCCCAGAGAAGGAGTTGTTTTCTTTCGTGTGGATCGTTGATTTTCCCCTCTTTGAAT
GGAACCCAGCGGAAAACAAGTGGGACCCTGCTCATCACATGTTCTCTGCTCCTCAGGAACAGTATCTTGAGACGCTCGAG
CAAGATCCCGGTTCGGTAAAAGGTGACCTCTATGATTTGGTGCTCAACGGGTATGAGCTGGCTTCAGGCTCAATTCGTAT
CCACGACACACAGCTGCAAAAACGCATCTTTAAGATAGTGGGATTAGATCCTGAAGAAGCGGGGGAAAAGTTCGGGTTTC
TCACAGAAGCGTTTAAATACGGCGCGCCGCCGCACGGCGGCATCGCACACGGGTTGGACCGCCTCGTGATGCTCATGACA
GGAAGCGAGTCAATTAGAGACGTCATTGCTTTTCCTAAAAATACACTCGCCGCCAGCCCCCTGGACAATTGTCCTAGCGT
GCTCGATAAGCGTCAGCTTGACGAGTTACACCTCACTGTACACGTCTAG

Upstream 100 bases:

>100_bases
AGACCGCGCGCGAAGTGTAGCACAGACTATGCAGCACAGTAAACCAACCGGAACAGGTGGTACACGCTGCCCGATGAACA
CCAGACAAAAGAACCCGTGA

Downstream 100 bases:

>100_bases
GGGCATCGCTACTCGCTCGTCGGCGTAAAATACCTACCAGGGGGGGGAGGGGTACATGGCTTTTACTGAGAAGCAAAAGG
GTACTTTGTGCCTAATGTGC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MYSAHTPWYDGQVMDYPRRTIACGELRRCHVGTVVVLNGWVHRKRSHGTVSFFNMRDRSGIVQVIVSQEENASLWSTVNR
IRLECCLAVEGVVRERPPSMINRALHTGEVEVHARTLYVLSENAVLPFRVDDVVHAHEDIRLKYRYLDLRSQRMQERIAL
RSRVALAIRQFLSMKGFIEIETPTFICSTPEGARDFVVPSRVCPGRFYALPQSPQLYKQLLMVAGFDRYFQLARCYRDED
ARGDRQPEFTQIDLEMSFVSRDDVMRVNEDMLRYVFRTSIGVELPTFFPRLTYAQALDQYGTDKPDMRFKPVLQNADFMG
MLGTFTPFEEVVAQGGSIRALVLPGKARCYSRRQIEALESIARAHEAHHLFWLKATGGGLEGGIARFFAGVESEVRRRLS
AQDEDLLLFVADCRHRVCCVALGAVRSALIRDESFPEKELFSFVWIVDFPLFEWNPAENKWDPAHHMFSAPQEQYLETLE
QDPGSVKGDLYDLVLNGYELASGSIRIHDTQLQKRIFKIVGLDPEEAGEKFGFLTEAFKYGAPPHGGIAHGLDRLVMLMT
GSESIRDVIAFPKNTLAASPLDNCPSVLDKRQLDELHLTVHV

Sequences:

>Translated_602_residues
MYSAHTPWYDGQVMDYPRRTIACGELRRCHVGTVVVLNGWVHRKRSHGTVSFFNMRDRSGIVQVIVSQEENASLWSTVNR
IRLECCLAVEGVVRERPPSMINRALHTGEVEVHARTLYVLSENAVLPFRVDDVVHAHEDIRLKYRYLDLRSQRMQERIAL
RSRVALAIRQFLSMKGFIEIETPTFICSTPEGARDFVVPSRVCPGRFYALPQSPQLYKQLLMVAGFDRYFQLARCYRDED
ARGDRQPEFTQIDLEMSFVSRDDVMRVNEDMLRYVFRTSIGVELPTFFPRLTYAQALDQYGTDKPDMRFKPVLQNADFMG
MLGTFTPFEEVVAQGGSIRALVLPGKARCYSRRQIEALESIARAHEAHHLFWLKATGGGLEGGIARFFAGVESEVRRRLS
AQDEDLLLFVADCRHRVCCVALGAVRSALIRDESFPEKELFSFVWIVDFPLFEWNPAENKWDPAHHMFSAPQEQYLETLE
QDPGSVKGDLYDLVLNGYELASGSIRIHDTQLQKRIFKIVGLDPEEAGEKFGFLTEAFKYGAPPHGGIAHGLDRLVMLMT
GSESIRDVIAFPKNTLAASPLDNCPSVLDKRQLDELHLTVHV
>Mature_602_residues
MYSAHTPWYDGQVMDYPRRTIACGELRRCHVGTVVVLNGWVHRKRSHGTVSFFNMRDRSGIVQVIVSQEENASLWSTVNR
IRLECCLAVEGVVRERPPSMINRALHTGEVEVHARTLYVLSENAVLPFRVDDVVHAHEDIRLKYRYLDLRSQRMQERIAL
RSRVALAIRQFLSMKGFIEIETPTFICSTPEGARDFVVPSRVCPGRFYALPQSPQLYKQLLMVAGFDRYFQLARCYRDED
ARGDRQPEFTQIDLEMSFVSRDDVMRVNEDMLRYVFRTSIGVELPTFFPRLTYAQALDQYGTDKPDMRFKPVLQNADFMG
MLGTFTPFEEVVAQGGSIRALVLPGKARCYSRRQIEALESIARAHEAHHLFWLKATGGGLEGGIARFFAGVESEVRRRLS
AQDEDLLLFVADCRHRVCCVALGAVRSALIRDESFPEKELFSFVWIVDFPLFEWNPAENKWDPAHHMFSAPQEQYLETLE
QDPGSVKGDLYDLVLNGYELASGSIRIHDTQLQKRIFKIVGLDPEEAGEKFGFLTEAFKYGAPPHGGIAHGLDRLVMLMT
GSESIRDVIAFPKNTLAASPLDNCPSVLDKRQLDELHLTVHV

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=607, Percent_Identity=40.8566721581549, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI45439306, Length=264, Percent_Identity=31.0606060606061, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI5031815, Length=271, Percent_Identity=27.3062730627306, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI194272210, Length=271, Percent_Identity=27.3062730627306, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI4758762, Length=303, Percent_Identity=27.3927392739274, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=43.1472081218274, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1790571, Length=291, Percent_Identity=26.8041237113402, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1789256, Length=291, Percent_Identity=27.1477663230241, Blast_Score=96, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI32566633, Length=602, Percent_Identity=34.5514950166113, Blast_Score=314, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17551876, Length=280, Percent_Identity=26.4285714285714, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71994340, Length=255, Percent_Identity=24.7058823529412, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=255, Percent_Identity=24.7058823529412, Blast_Score=79, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=255, Percent_Identity=24.7058823529412, Blast_Score=79, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=649, Percent_Identity=32.0493066255778, Blast_Score=262, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6323011, Length=274, Percent_Identity=26.6423357664234, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320242, Length=262, Percent_Identity=25.1908396946565, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24584738, Length=596, Percent_Identity=37.751677852349, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI17136276, Length=244, Percent_Identity=28.2786885245902, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24640849, Length=240, Percent_Identity=25.8333333333333, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24640851, Length=240, Percent_Identity=25.8333333333333, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19921528, Length=232, Percent_Identity=26.7241379310345, Blast_Score=66, Evalue=9e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_TREPA (O83950)

Other databases:

- EMBL:   AE000520
- PIR:   A71256
- RefSeq:   NP_219422.1
- ProteinModelPortal:   O83950
- SMR:   O83950
- IntAct:   O83950
- GeneID:   2611613
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0985
- NMPDR:   fig|243276.1.peg.980
- TIGR:   TP_0985
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   TPAL243276:TP_0985-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 68627; Mature: 68627

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSAHTPWYDGQVMDYPRRTIACGELRRCHVGTVVVLNGWVHRKRSHGTVSFFNMRDRSG
CCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEECHHHCCCCCCCEEEEEECCCCC
IVQVIVSQEENASLWSTVNRIRLECCLAVEGVVRERPPSMINRALHTGEVEVHARTLYVL
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEEEEEE
SENAVLPFRVDDVVHAHEDIRLKYRYLDLRSQRMQERIALRSRVALAIRQFLSMKGFIEI
ECCCEEEEEHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
ETPTFICSTPEGARDFVVPSRVCPGRFYALPQSPQLYKQLLMVAGFDRYFQLARCYRDED
ECCEEEECCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCC
ARGDRQPEFTQIDLEMSFVSRDDVMRVNEDMLRYVFRTSIGVELPTFFPRLTYAQALDQY
CCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
GTDKPDMRFKPVLQNADFMGMLGTFTPFEEVVAQGGSIRALVLPGKARCYSRRQIEALES
CCCCCCCEEHHHHCCCCCHHHHCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHH
IARAHEAHHLFWLKATGGGLEGGIARFFAGVESEVRRRLSAQDEDLLLFVADCRHRVCCV
HHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCHHHHH
ALGAVRSALIRDESFPEKELFSFVWIVDFPLFEWNPAENKWDPAHHMFSAPQEQYLETLE
HHHHHHHHHHCCCCCCHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHH
QDPGSVKGDLYDLVLNGYELASGSIRIHDTQLQKRIFKIVGLDPEEAGEKFGFLTEAFKY
CCCCCCCCHHHHHHHCCEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
GAPPHGGIAHGLDRLVMLMTGSESIRDVIAFPKNTLAASPLDNCPSVLDKRQLDELHLTV
CCCCCCCHHHHHHHHEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCEEEEEE
HV
EC
>Mature Secondary Structure
MYSAHTPWYDGQVMDYPRRTIACGELRRCHVGTVVVLNGWVHRKRSHGTVSFFNMRDRSG
CCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEECHHHCCCCCCCEEEEEECCCCC
IVQVIVSQEENASLWSTVNRIRLECCLAVEGVVRERPPSMINRALHTGEVEVHARTLYVL
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEEEEEE
SENAVLPFRVDDVVHAHEDIRLKYRYLDLRSQRMQERIALRSRVALAIRQFLSMKGFIEI
ECCCEEEEEHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
ETPTFICSTPEGARDFVVPSRVCPGRFYALPQSPQLYKQLLMVAGFDRYFQLARCYRDED
ECCEEEECCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCC
ARGDRQPEFTQIDLEMSFVSRDDVMRVNEDMLRYVFRTSIGVELPTFFPRLTYAQALDQY
CCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
GTDKPDMRFKPVLQNADFMGMLGTFTPFEEVVAQGGSIRALVLPGKARCYSRRQIEALES
CCCCCCCEEHHHHCCCCCHHHHCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHH
IARAHEAHHLFWLKATGGGLEGGIARFFAGVESEVRRRLSAQDEDLLLFVADCRHRVCCV
HHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCHHHHH
ALGAVRSALIRDESFPEKELFSFVWIVDFPLFEWNPAENKWDPAHHMFSAPQEQYLETLE
HHHHHHHHHHCCCCCCHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHH
QDPGSVKGDLYDLVLNGYELASGSIRIHDTQLQKRIFKIVGLDPEEAGEKFGFLTEAFKY
CCCCCCCCHHHHHHHCCEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
GAPPHGGIAHGLDRLVMLMTGSESIRDVIAFPKNTLAASPLDNCPSVLDKRQLDELHLTV
CCCCCCCHHHHHHHHEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCEEEEEE
HV
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876