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The map label for this gene is gltX

Identifier: 189025898

GI number: 189025898

Start: 739502

End: 741115

Strand: Reverse

Name: gltX

Synonym: TPASS_0673

Alternate gene names: 189025898

Gene position: 741115-739502 (Counterclockwise)

Preceding gene: 189025901

Following gene: 189025897

Centisome position: 65.04

GC content: 56.32

Gene sequence:

>1614_bases
GTGGAGGTGAGAGTTCGGTACGCACCGTCTCCGACGGGGCTCCAGCACATCGGGGGTATTAGAACTGCTCTCTTCAACTT
CTTGTTCGCGCGAGCGCATGCAGGCGTATTTGTCCTCCGTGTCGAGGATACTGACCGCAGTCGCTGCACTGCAGCGTTTG
AGCAGAACCTTTACGATACGCTCCGTTGGCTTGGGGTCTCCTGGGATGAGGGGGGAGGGTGCCCAGAAACAGCGGTGAAG
CAGGGCGCGCGGGGGGATGGCCGCTCTGTTGCTCACGCTGGTGGGGCCTATGGCCCTTACACGCAGTCTGCACGGACAGA
TCTCTACCGCGCGCAGGTGGCGCGGCTCGTTGAGACAGGGCAGGCGTATTATTGTTTTTGCGATGCGTCGCGGCTCGAGC
GCGTTCGTAAGATCCGTACGCTCAACAGGATGCCCCCCGGTTATGACCGGCATTGCCGCGAGCTCCTGCCTGAAGAAGTT
CGGGAATGTCTCGCATCCGGGGTTCCACATGTGATCCGCTTTAAGGTCCCCTTGGAAGGGAGTACTCATTTCCGCGATGC
GCTGCTCGGTGATATCGAGTGGCAAAATGAGGAGATCAATCCAGACCCGATTTTACTGAAAAGCGACGGGTTCCCCACTT
ACCATTTGGCTAATGTGGTAGATGACCATGCTATGCGTATTACGCATGTTTTGCGCGCTCAGGAGTGGGTTCCCTCCACC
CCGTTACACCTTCTGTTGTACCGTGCTTTTGGCTGGCAGCCCCCGCTCTTCTGTCATCTTCCGATGGTTATGGGGGCAGA
TGGGCACAAGTTGTCAAAGCGGCATGGAGCTACTAGCTGTGATGAGTTCCGCAACGCGGGGTATTTGCCTGAAGCGTTGC
TCAACTATGTTGCAATGCTCGGTTGCTCGTACGGAGAAGGTCAGGATCTGTTCACGCGAGAGCAGCTGTGTGCGCACTTT
TCTCTGTCGCGTTTAAATAAGTCACCGGCTGTTTTTGACTATAAAAAGCTTGCGTGGTTTAACGGTCAATATATCCGTGC
AAAAAGTGACGAGCAGCTGTGTGCGCTCGTGTGGCCTTTCATTGCAAACGCCGGTGTGTGTGGCCACATTCCGGCAGATG
TGGAAGCAGGAGCTGTGCGCACACGACGTTTTGCAGACGAGGCGCCGTGTGCGCCTACAGAAGCGCAGCGTTCCATGCTC
ATGCGAGTTATCCCGCTGATTAAGGAGCGGTTGCGGTTTCTAACCGATGCGCCGGAGTTGGTGCGTTGTTTTTTTCAAGA
ACCGTCTCTCCCTGAACAAGGGGTGTTTGTGCCGAAGCGCTTGGATGTTGCGCAGGTGCGCGCGGTACTGGTGCGCGCCA
GGGGCCTGGTGCACGAAATAGTGAGTGCCAGTGAACCGGATGTTGAGGTGCTCTTGCGTGCTGAGGCAGAAAAGTTTGGA
ATAAAACTTGGTGATTTTCTCATGCCCATTCGCGTTGCGCTCACCGGTGCTACCGTGAGTGCCCCTCTGGTAGGAACTAT
CCGCATCCTGGGGGCGTCACGATCCTGTGCGCGTATTGAACACGTCATTCGTGAACGCTTTTCGGATGACAGTCAAGGAG
TGGGAGGAGGCTGA

Upstream 100 bases:

>100_bases
GCGGTCACGGTGCATGAGGAGTGTAATTTTCAGGGCCTCACAGGGATGCTTGCGCCCCGGAGGGGGATGTGCGATAATCG
GCCCCCGGGGAGGGCGAGCG

Downstream 100 bases:

>100_bases
TATTCTCAGTTAACGCGGGCTATAGGGAAAAGAGGTATGCAGGGGATGTGTTCAAAGGCGGGCGCGATCTCTGTGAGGTC
GCCGCGGGTGCTGACAGTAT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MEVRVRYAPSPTGLQHIGGIRTALFNFLFARAHAGVFVLRVEDTDRSRCTAAFEQNLYDTLRWLGVSWDEGGGCPETAVK
QGARGDGRSVAHAGGAYGPYTQSARTDLYRAQVARLVETGQAYYCFCDASRLERVRKIRTLNRMPPGYDRHCRELLPEEV
RECLASGVPHVIRFKVPLEGSTHFRDALLGDIEWQNEEINPDPILLKSDGFPTYHLANVVDDHAMRITHVLRAQEWVPST
PLHLLLYRAFGWQPPLFCHLPMVMGADGHKLSKRHGATSCDEFRNAGYLPEALLNYVAMLGCSYGEGQDLFTREQLCAHF
SLSRLNKSPAVFDYKKLAWFNGQYIRAKSDEQLCALVWPFIANAGVCGHIPADVEAGAVRTRRFADEAPCAPTEAQRSML
MRVIPLIKERLRFLTDAPELVRCFFQEPSLPEQGVFVPKRLDVAQVRAVLVRARGLVHEIVSASEPDVEVLLRAEAEKFG
IKLGDFLMPIRVALTGATVSAPLVGTIRILGASRSCARIEHVIRERFSDDSQGVGGG

Sequences:

>Translated_537_residues
MEVRVRYAPSPTGLQHIGGIRTALFNFLFARAHAGVFVLRVEDTDRSRCTAAFEQNLYDTLRWLGVSWDEGGGCPETAVK
QGARGDGRSVAHAGGAYGPYTQSARTDLYRAQVARLVETGQAYYCFCDASRLERVRKIRTLNRMPPGYDRHCRELLPEEV
RECLASGVPHVIRFKVPLEGSTHFRDALLGDIEWQNEEINPDPILLKSDGFPTYHLANVVDDHAMRITHVLRAQEWVPST
PLHLLLYRAFGWQPPLFCHLPMVMGADGHKLSKRHGATSCDEFRNAGYLPEALLNYVAMLGCSYGEGQDLFTREQLCAHF
SLSRLNKSPAVFDYKKLAWFNGQYIRAKSDEQLCALVWPFIANAGVCGHIPADVEAGAVRTRRFADEAPCAPTEAQRSML
MRVIPLIKERLRFLTDAPELVRCFFQEPSLPEQGVFVPKRLDVAQVRAVLVRARGLVHEIVSASEPDVEVLLRAEAEKFG
IKLGDFLMPIRVALTGATVSAPLVGTIRILGASRSCARIEHVIRERFSDDSQGVGGG
>Mature_537_residues
MEVRVRYAPSPTGLQHIGGIRTALFNFLFARAHAGVFVLRVEDTDRSRCTAAFEQNLYDTLRWLGVSWDEGGGCPETAVK
QGARGDGRSVAHAGGAYGPYTQSARTDLYRAQVARLVETGQAYYCFCDASRLERVRKIRTLNRMPPGYDRHCRELLPEEV
RECLASGVPHVIRFKVPLEGSTHFRDALLGDIEWQNEEINPDPILLKSDGFPTYHLANVVDDHAMRITHVLRAQEWVPST
PLHLLLYRAFGWQPPLFCHLPMVMGADGHKLSKRHGATSCDEFRNAGYLPEALLNYVAMLGCSYGEGQDLFTREQLCAHF
SLSRLNKSPAVFDYKKLAWFNGQYIRAKSDEQLCALVWPFIANAGVCGHIPADVEAGAVRTRRFADEAPCAPTEAQRSML
MRVIPLIKERLRFLTDAPELVRCFFQEPSLPEQGVFVPKRLDVAQVRAVLVRARGLVHEIVSASEPDVEVLLRAEAEKFG
IKLGDFLMPIRVALTGATVSAPLVGTIRILGASRSCARIEHVIRERFSDDSQGVGGG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=537, Percent_Identity=34.0782122905028, Blast_Score=285, Evalue=1e-76,
Organism=Escherichia coli, GI1788743, Length=523, Percent_Identity=35.1816443594646, Blast_Score=307, Evalue=9e-85,
Organism=Escherichia coli, GI226510925, Length=272, Percent_Identity=31.9852941176471, Blast_Score=120, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71993394, Length=334, Percent_Identity=39.8203592814371, Blast_Score=254, Evalue=9e-68,
Organism=Saccharomyces cerevisiae, GI6324540, Length=534, Percent_Identity=33.8951310861423, Blast_Score=278, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24665321, Length=529, Percent_Identity=35.7277882797732, Blast_Score=301, Evalue=6e-82,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_TREPA (O83679)

Other databases:

- EMBL:   AE000520
- PIR:   D71296
- RefSeq:   NP_219110.1
- ProteinModelPortal:   O83679
- SMR:   O83679
- IntAct:   O83679
- GeneID:   2611576
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0673
- NMPDR:   fig|243276.1.peg.669
- TIGR:   TP_0673
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BioCyc:   TPAL243276:TP_0673-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 59840; Mature: 59840

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVRVRYAPSPTGLQHIGGIRTALFNFLFARAHAGVFVLRVEDTDRSRCTAAFEQNLYDT
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHH
LRWLGVSWDEGGGCPETAVKQGARGDGRSVAHAGGAYGPYTQSARTDLYRAQVARLVETG
HHHHCCCCCCCCCCCHHHHHCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
QAYYCFCDASRLERVRKIRTLNRMPPGYDRHCRELLPEEVRECLASGVPHVIRFKVPLEG
CEEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
STHFRDALLGDIEWQNEEINPDPILLKSDGFPTYHLANVVDDHAMRITHVLRAQEWVPST
CCHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLHLLLYRAFGWQPPLFCHLPMVMGADGHKLSKRHGATSCDEFRNAGYLPEALLNYVAML
HHHHHHHHHHCCCCCHHEECHHHCCCCCCHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHH
GCSYGEGQDLFTREQLCAHFSLSRLNKSPAVFDYKKLAWFNGQYIRAKSDEQLCALVWPF
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEECCCEEEECCCCHHHHHHHHH
IANAGVCGHIPADVEAGAVRTRRFADEAPCAPTEAQRSMLMRVIPLIKERLRFLTDAPEL
HHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHH
VRCFFQEPSLPEQGVFVPKRLDVAQVRAVLVRARGLVHEIVSASEPDVEVLLRAEAEKFG
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
IKLGDFLMPIRVALTGATVSAPLVGTIRILGASRSCARIEHVIRERFSDDSQGVGGG
CCHHHHHHHHHHHEECCCCCCCHHEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MEVRVRYAPSPTGLQHIGGIRTALFNFLFARAHAGVFVLRVEDTDRSRCTAAFEQNLYDT
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHH
LRWLGVSWDEGGGCPETAVKQGARGDGRSVAHAGGAYGPYTQSARTDLYRAQVARLVETG
HHHHCCCCCCCCCCCHHHHHCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
QAYYCFCDASRLERVRKIRTLNRMPPGYDRHCRELLPEEVRECLASGVPHVIRFKVPLEG
CEEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
STHFRDALLGDIEWQNEEINPDPILLKSDGFPTYHLANVVDDHAMRITHVLRAQEWVPST
CCHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLHLLLYRAFGWQPPLFCHLPMVMGADGHKLSKRHGATSCDEFRNAGYLPEALLNYVAML
HHHHHHHHHHCCCCCHHEECHHHCCCCCCHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHH
GCSYGEGQDLFTREQLCAHFSLSRLNKSPAVFDYKKLAWFNGQYIRAKSDEQLCALVWPF
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEECCCEEEECCCCHHHHHHHHH
IANAGVCGHIPADVEAGAVRTRRFADEAPCAPTEAQRSMLMRVIPLIKERLRFLTDAPEL
HHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHH
VRCFFQEPSLPEQGVFVPKRLDVAQVRAVLVRARGLVHEIVSASEPDVEVLLRAEAEKFG
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
IKLGDFLMPIRVALTGATVSAPLVGTIRILGASRSCARIEHVIRERFSDDSQGVGGG
CCHHHHHHHHHHHEECCCCCCCHHEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876