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The map label for this gene is glyS

Identifier: 189025897

GI number: 189025897

Start: 737979

End: 739367

Strand: Reverse

Name: glyS

Synonym: TPASS_0672

Alternate gene names: 189025897

Gene position: 739367-737979 (Counterclockwise)

Preceding gene: 189025898

Following gene: 189025896

Centisome position: 64.89

GC content: 51.91

Gene sequence:

>1389_bases
ATGGAGAAGATTGTCGGTCTCTGCAAACGGCGTGGCTTTGTGTTTCCATCTTCAGAAATTTATGGTGGCCAAGGAGGTGT
TTGGGACTACGGCCCTATGGGCATTGCGCTAAAAAACAATATTGCCCATGCCTGGTGGCAAGATATGACACGCCTACATG
ATCATATCGTCGGGCTGGATGCAGCAATCTTGATGCATCCAAACGTATGGCGGACGTCTGGCCACGTCGATCACTTCAGT
GATCCTTTGGTTGATTGCACGGTGTGTAAAAGTCGCTTTCGCGCGGATCAGGTTGCCGTGCCGTCTGCCGGGGGACCCTG
TCCTCAGTGTGGTGGGGCCCTCACGGGCGTGCGTAATTTTAACCTCATGTTCAGTACCCACATGGGTCCTACGGATGAGC
GTGCCAGTTTGCTCTACCTGCGTCCTGAAACTGCGCAGGGGATTTATGTAAATTATAAAAACGTCCTGCAAACTACACGC
CTGAAGGTGCCTTTTGGTATTGCCCAGATCGGTAAGGCGTTTCGCAATGAGATTGTCACAAAAAACTTTATTTTCCGTAC
GTGTGAATTTGAACAAATGGAAATGCAGTTTTTTGTGCGCCCCGCAGAGGATACTCACTGGTTTGAGTACTGGTGTGCAC
AGCGCTGGGCTTTTTACCAAAAGTACGGGGTGCGTATGAACCACATGCGTTGGCGTACCCATGCTGCACATGAGTTGGCT
CATTATGCACGGGCTGCCTGTGACATTGAGTATGCATTCCCTATGGGCTTTAGGGAATTAGAAGGGGTGCATAACCGTGG
TGACTTTGACCTGACGCGCCACGCGCAGCACTCGGGTAAAGACTTGTGCTATGTGGATCCTGATCCAAACCTGGATGCGG
CAGCGCGTCGGTATGTGCCTTGTGTCGTTGAAACGTCTGCAGGATTGACGCGCTGCGTACTCATGTTTCTGTGCGATGCA
TACACAGAAGAATATGTGCAGGCGCCGAATGTCGCGTTTTCGGAAACGACACAGACAGCTGATCAAGAAGGTGCTGCACG
TACGGGCGAGATGCGAATAGTGCTGAGGTTGCACCCTGCGCTTTCTCCCACCACTGTTGCTTTTTTGCCTTTGGTAAAAA
AAGACGGATTGGTTGACCTTGCGCGTGCGGTGCGCGACGAGCTGCGTGAGGATTTTGCCTGTGATTTTGATGCAGCTGGC
GCGATTGGAAAGCGCTACCGCCGTCAAGACGAGGTGGGTACTCCCTTTTGTGTCACAGTTGATTATCAGTCAAAGGAAGA
TGATACGGTTACGGTACGTCTGCGCGACAGCATGGCACAGCGCCGGGTCTCTCGTGCCTTTCTTGCAGAGTTTTTGCGCA
CAGAGATAAAACACTACCGGCGTCCCTAG

Upstream 100 bases:

>100_bases
GTATGCAGGGGATGTGTTCAAAGGCGGGCGCGATCTCTGTGAGGTCGCCGCGGGTGCTGACAGTATGCTAGACAGGGGGG
AAGGTGAGCGGCGCGTCACC

Downstream 100 bases:

>100_bases
GTTGTTGTCCGCTCTCTGCGCGCGGGGAAAATGTCACATATTACATCGCGAAGGAGCTCTCGTATGAAAGCGTATTCTTA
TGCAGTAGAGGATCGCTCGC

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase; GlyRS

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MEKIVGLCKRRGFVFPSSEIYGGQGGVWDYGPMGIALKNNIAHAWWQDMTRLHDHIVGLDAAILMHPNVWRTSGHVDHFS
DPLVDCTVCKSRFRADQVAVPSAGGPCPQCGGALTGVRNFNLMFSTHMGPTDERASLLYLRPETAQGIYVNYKNVLQTTR
LKVPFGIAQIGKAFRNEIVTKNFIFRTCEFEQMEMQFFVRPAEDTHWFEYWCAQRWAFYQKYGVRMNHMRWRTHAAHELA
HYARAACDIEYAFPMGFRELEGVHNRGDFDLTRHAQHSGKDLCYVDPDPNLDAAARRYVPCVVETSAGLTRCVLMFLCDA
YTEEYVQAPNVAFSETTQTADQEGAARTGEMRIVLRLHPALSPTTVAFLPLVKKDGLVDLARAVRDELREDFACDFDAAG
AIGKRYRRQDEVGTPFCVTVDYQSKEDDTVTVRLRDSMAQRRVSRAFLAEFLRTEIKHYRRP

Sequences:

>Translated_462_residues
MEKIVGLCKRRGFVFPSSEIYGGQGGVWDYGPMGIALKNNIAHAWWQDMTRLHDHIVGLDAAILMHPNVWRTSGHVDHFS
DPLVDCTVCKSRFRADQVAVPSAGGPCPQCGGALTGVRNFNLMFSTHMGPTDERASLLYLRPETAQGIYVNYKNVLQTTR
LKVPFGIAQIGKAFRNEIVTKNFIFRTCEFEQMEMQFFVRPAEDTHWFEYWCAQRWAFYQKYGVRMNHMRWRTHAAHELA
HYARAACDIEYAFPMGFRELEGVHNRGDFDLTRHAQHSGKDLCYVDPDPNLDAAARRYVPCVVETSAGLTRCVLMFLCDA
YTEEYVQAPNVAFSETTQTADQEGAARTGEMRIVLRLHPALSPTTVAFLPLVKKDGLVDLARAVRDELREDFACDFDAAG
AIGKRYRRQDEVGTPFCVTVDYQSKEDDTVTVRLRDSMAQRRVSRAFLAEFLRTEIKHYRRP
>Mature_462_residues
MEKIVGLCKRRGFVFPSSEIYGGQGGVWDYGPMGIALKNNIAHAWWQDMTRLHDHIVGLDAAILMHPNVWRTSGHVDHFS
DPLVDCTVCKSRFRADQVAVPSAGGPCPQCGGALTGVRNFNLMFSTHMGPTDERASLLYLRPETAQGIYVNYKNVLQTTR
LKVPFGIAQIGKAFRNEIVTKNFIFRTCEFEQMEMQFFVRPAEDTHWFEYWCAQRWAFYQKYGVRMNHMRWRTHAAHELA
HYARAACDIEYAFPMGFRELEGVHNRGDFDLTRHAQHSGKDLCYVDPDPNLDAAARRYVPCVVETSAGLTRCVLMFLCDA
YTEEYVQAPNVAFSETTQTADQEGAARTGEMRIVLRLHPALSPTTVAFLPLVKKDGLVDLARAVRDELREDFACDFDAAG
AIGKRYRRQDEVGTPFCVTVDYQSKEDDTVTVRLRDSMAQRRVSRAFLAEFLRTEIKHYRRP

Specific function: Catalyzes the attachment of glycine to tRNA(Gly)

COG id: COG0423

COG function: function code J; Glycyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI116805340, Length=588, Percent_Identity=26.7006802721088, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI70887790, Length=483, Percent_Identity=21.1180124223602, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17554996, Length=599, Percent_Identity=27.212020033389, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI32564541, Length=599, Percent_Identity=27.212020033389, Blast_Score=171, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6325338, Length=365, Percent_Identity=27.3972602739726, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6319597, Length=219, Percent_Identity=31.5068493150685, Blast_Score=110, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21357965, Length=385, Percent_Identity=30.1298701298701, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24664462, Length=385, Percent_Identity=30.1298701298701, Blast_Score=156, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYG_TREPA (O83678)

Other databases:

- EMBL:   AE000520
- PIR:   C71296
- RefSeq:   NP_219109.1
- ProteinModelPortal:   O83678
- GeneID:   2611575
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0672
- NMPDR:   fig|243276.1.peg.668
- TIGR:   TP_0672
- HOGENOM:   HBG319978
- OMA:   GSEWGEL
- ProtClustDB:   PRK04173
- BioCyc:   TPAL243276:TP_0672-MONOMER
- BRENDA:   6.1.1.14
- GO:   GO:0005737
- HAMAP:   MF_00253_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR022961
- InterPro:   IPR002315
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR10745
- PRINTS:   PR01043
- TIGRFAMs:   TIGR00389

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.14

Molecular weight: Translated: 52678; Mature: 52678

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 94-94 BINDING 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIVGLCKRRGFVFPSSEIYGGQGGVWDYGPMGIALKNNIAHAWWQDMTRLHDHIVGLD
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCC
AAILMHPNVWRTSGHVDHFSDPLVDCTVCKSRFRADQVAVPSAGGPCPQCGGALTGVRNF
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEE
NLMFSTHMGPTDERASLLYLRPETAQGIYVNYKNVLQTTRLKVPFGIAQIGKAFRNEIVT
EEEEEECCCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
KNFIFRTCEFEQMEMQFFVRPAEDTHWFEYWCAQRWAFYQKYGVRMNHMRWRTHAAHELA
HHHHEEECCHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHH
HYARAACDIEYAFPMGFRELEGVHNRGDFDLTRHAQHSGKDLCYVDPDPNLDAAARRYVP
HHHHHHCCEEEECCCCHHHHHCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHCCC
CVVETSAGLTRCVLMFLCDAYTEEYVQAPNVAFSETTQTADQEGAARTGEMRIVLRLHPA
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHCCCCCCCCEEEEEEECCC
LSPTTVAFLPLVKKDGLVDLARAVRDELREDFACDFDAAGAIGKRYRRQDEVGTPFCVTV
CCCCHHHEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEE
DYQSKEDDTVTVRLRDSMAQRRVSRAFLAEFLRTEIKHYRRP
ECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKIVGLCKRRGFVFPSSEIYGGQGGVWDYGPMGIALKNNIAHAWWQDMTRLHDHIVGLD
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCC
AAILMHPNVWRTSGHVDHFSDPLVDCTVCKSRFRADQVAVPSAGGPCPQCGGALTGVRNF
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEE
NLMFSTHMGPTDERASLLYLRPETAQGIYVNYKNVLQTTRLKVPFGIAQIGKAFRNEIVT
EEEEEECCCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
KNFIFRTCEFEQMEMQFFVRPAEDTHWFEYWCAQRWAFYQKYGVRMNHMRWRTHAAHELA
HHHHEEECCHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHH
HYARAACDIEYAFPMGFRELEGVHNRGDFDLTRHAQHSGKDLCYVDPDPNLDAAARRYVP
HHHHHHCCEEEECCCCHHHHHCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHCCC
CVVETSAGLTRCVLMFLCDAYTEEYVQAPNVAFSETTQTADQEGAARTGEMRIVLRLHPA
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHCCCCCCCCEEEEEEECCC
LSPTTVAFLPLVKKDGLVDLARAVRDELREDFACDFDAAGAIGKRYRRQDEVGTPFCVTV
CCCCHHHEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEE
DYQSKEDDTVTVRLRDSMAQRRVSRAFLAEFLRTEIKHYRRP
ECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876