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The map label for this gene is potD [H]

Identifier: 189025880

GI number: 189025880

Start: 720048

End: 721094

Strand: Direct

Name: potD [H]

Synonym: TPASS_0655

Alternate gene names: 189025880

Gene position: 720048-721094 (Clockwise)

Preceding gene: 189025879

Following gene: 189025881

Centisome position: 63.19

GC content: 47.56

Gene sequence:

>1047_bases
ATGAAACGTTTTTGTGTGAGCAGTTCGCGCATTCTTTCTCTCCTTTTTCTCTCCCTCTGGATGGGATCATGCCTGCAGAC
ACGACAGGATGTCCTGTACCTGTATAACTGGACGTACTACACCCCGACGTCCCTCATTAAAAAGTTTGAACAACAGTATA
ACGTGCAGGTAGTCTATGATGATTACGCTTCAAATGAAGATATGTTTGCAAAACTGAGCATTGGTGCAAGTGGTTATGAT
TTAGTGGTCCCTTCGGGTGACTTTGTCAGCATCATGAAACGCAAGCATCTGTTGGAAAAAATTGACCTATCCAAGATTCC
CAACGTACAGTTTATCAAAGAGAGTGTCCGTGCTCGCATAGCCTACGATCCAAAAATGGAATATTCGGTGCCGTATTATC
TTGGAGCGGCAGGCATTGCGGTAAACAAAAAAGCAGTTCCGTCATACGCGCGCACGTGGTCTATCTTCTCACGCAAAGAC
CTGGCGTATCGTATGAGCATGATGGACGATATGCGCGAAGTAATGGGTGCTGCACTTGCTTCACTTGGCTACAACGTAAA
CACAAAAAACGAACAAGAGCTTGCGCAGGCAGCAATACTTGTCACCGACCACTGGAAACCAAACTTGGTAAAGTTTGATT
CGGACGGATACGCAAAGTCATTTGCATCGGGAGACTTTGTTGTAGCTCATGGCTTTGCAGAGGCCTTCTTTGCAGAGACT
CCTGAGGCAATGCACGAACATATAGACTTCTTTATACCACAAGATGTCGCCAGCCCAGTGTACGTAGACAGCTTTTGCAT
TCCCAAGGGGGCACGCAATCGCGACCTTGCCCATGCGTTTATCAACTTCTTCCTAGAGCCTGCACACTACGCGGAGTTTT
TGGACACCTTTGGATTCCCTTCAACTATCCACCGGGAAGCGGCAGCATATCAGAAGAAGACTCCATACTACAGCGAGCAT
GACCTCGAGCGAGGAACGCTAAAAACAGATGTGGGGGCAGCAATCGAACACTACAATGCGCACTGGAACGCGGTGCGATT
TAGGTGA

Upstream 100 bases:

>100_bases
CAGGGATTGTAGGGGTCGCCTACCTCCTGCGCAATTCTTTAAAAACAATAGTCCATTCTAAGTAACTACTTTCTGCAAAC
AATCTCGTAAGGAGCAAAGC

Downstream 100 bases:

>100_bases
ACTCAGTCATCCGCGCAAAACACTCCCACCCCGAGGGATGGGTATCCTCTGCAACTACACAGGCAACTGAGGCAATGAGT
TAGGAGACCAGGGGGAGTCG

Product: spermidine/putrescine ABC transporter, periplasmic binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKRFCVSSSRILSLLFLSLWMGSCLQTRQDVLYLYNWTYYTPTSLIKKFEQQYNVQVVYDDYASNEDMFAKLSIGASGYD
LVVPSGDFVSIMKRKHLLEKIDLSKIPNVQFIKESVRARIAYDPKMEYSVPYYLGAAGIAVNKKAVPSYARTWSIFSRKD
LAYRMSMMDDMREVMGAALASLGYNVNTKNEQELAQAAILVTDHWKPNLVKFDSDGYAKSFASGDFVVAHGFAEAFFAET
PEAMHEHIDFFIPQDVASPVYVDSFCIPKGARNRDLAHAFINFFLEPAHYAEFLDTFGFPSTIHREAAAYQKKTPYYSEH
DLERGTLKTDVGAAIEHYNAHWNAVRFR

Sequences:

>Translated_348_residues
MKRFCVSSSRILSLLFLSLWMGSCLQTRQDVLYLYNWTYYTPTSLIKKFEQQYNVQVVYDDYASNEDMFAKLSIGASGYD
LVVPSGDFVSIMKRKHLLEKIDLSKIPNVQFIKESVRARIAYDPKMEYSVPYYLGAAGIAVNKKAVPSYARTWSIFSRKD
LAYRMSMMDDMREVMGAALASLGYNVNTKNEQELAQAAILVTDHWKPNLVKFDSDGYAKSFASGDFVVAHGFAEAFFAET
PEAMHEHIDFFIPQDVASPVYVDSFCIPKGARNRDLAHAFINFFLEPAHYAEFLDTFGFPSTIHREAAAYQKKTPYYSEH
DLERGTLKTDVGAAIEHYNAHWNAVRFR
>Mature_348_residues
MKRFCVSSSRILSLLFLSLWMGSCLQTRQDVLYLYNWTYYTPTSLIKKFEQQYNVQVVYDDYASNEDMFAKLSIGASGYD
LVVPSGDFVSIMKRKHLLEKIDLSKIPNVQFIKESVRARIAYDPKMEYSVPYYLGAAGIAVNKKAVPSYARTWSIFSRKD
LAYRMSMMDDMREVMGAALASLGYNVNTKNEQELAQAAILVTDHWKPNLVKFDSDGYAKSFASGDFVVAHGFAEAFFAET
PEAMHEHIDFFIPQDVASPVYVDSFCIPKGARNRDLAHAFINFFLEPAHYAEFLDTFGFPSTIHREAAAYQKKTPYYSEH
DLERGTLKTDVGAAIEHYNAHWNAVRFR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=339, Percent_Identity=31.858407079646, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI1787078, Length=289, Percent_Identity=27.681660899654, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39753; Mature: 39753

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFCVSSSRILSLLFLSLWMGSCLQTRQDVLYLYNWTYYTPTSLIKKFEQQYNVQVVYD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCEECHHHHHHHHHHHCCEEEEEC
DYASNEDMFAKLSIGASGYDLVVPSGDFVSIMKRKHLLEKIDLSKIPNVQFIKESVRARI
CCCCCCCEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEE
AYDPKMEYSVPYYLGAAGIAVNKKAVPSYARTWSIFSRKDLAYRMSMMDDMREVMGAALA
EECCCCCCCCCEEECCCCEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLGYNVNTKNEQELAQAAILVTDHWKPNLVKFDSDGYAKSFASGDFVVAHGFAEAFFAET
HHCCCCCCCCHHHHHHHHEEEECCCCCCEEEECCCCCHHHHCCCCEEEECHHHHHHHHCC
PEAMHEHIDFFIPQDVASPVYVDSFCIPKGARNRDLAHAFINFFLEPAHYAEFLDTFGFP
HHHHHHHHCEECCHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCC
STIHREAAAYQKKTPYYSEHDLERGTLKTDVGAAIEHYNAHWNAVRFR
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MKRFCVSSSRILSLLFLSLWMGSCLQTRQDVLYLYNWTYYTPTSLIKKFEQQYNVQVVYD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCEECHHHHHHHHHHHCCEEEEEC
DYASNEDMFAKLSIGASGYDLVVPSGDFVSIMKRKHLLEKIDLSKIPNVQFIKESVRARI
CCCCCCCEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEE
AYDPKMEYSVPYYLGAAGIAVNKKAVPSYARTWSIFSRKDLAYRMSMMDDMREVMGAALA
EECCCCCCCCCEEECCCCEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLGYNVNTKNEQELAQAAILVTDHWKPNLVKFDSDGYAKSFASGDFVVAHGFAEAFFAET
HHCCCCCCCCHHHHHHHHEEEECCCCCCEEEECCCCCHHHHCCCCEEEECHHHHHHHHCC
PEAMHEHIDFFIPQDVASPVYVDSFCIPKGARNRDLAHAFINFFLEPAHYAEFLDTFGFP
HHHHHHHHCEECCHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCC
STIHREAAAYQKKTPYYSEHDLERGTLKTDVGAAIEHYNAHWNAVRFR
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]