Click here to switch to the map view.

The map label for this gene is dnaQ

Identifier: 189025868

GI number: 189025868

Start: 706253

End: 706900

Strand: Direct

Name: dnaQ

Synonym: TPASS_0643

Alternate gene names: 189025868

Gene position: 706253-706900 (Clockwise)

Preceding gene: 189025867

Following gene: 189025877

Centisome position: 61.98

GC content: 46.6

Gene sequence:

>648_bases
ATGATATATGATTGGGTTTTTGCGGTGCATGAACACGTTGCATTCACCGCCTTCGACACCGAAACAACTGGTTTAAAAGC
AGAAGAAGATCGCATTATAGAAATTGGTGCAGTAACGTTTGATCGAAAGGGTATTATTGCGCGCTTTAGCACGCTCATCT
TCCCTGATCGGGCTATACCCCCTGACGTGAGCAAAATCAACCATATTACCGACGATATGCTCGTCAATAAGCCGCGCTTT
TGTGAGATAGTTTCAGACTTTTCTCGATTCATCAAAGGGACAGTACTCGTCGCACATAACGCAAACTTCGATGTCGAGTT
TCTCAATGCAGAACTCAGCCTGTGCAAAAAGCAACCCCTGTCTCACAAAGTAGTTGATACGTACGCGATGGCCCAAGCGG
TGTTCCCCGGGTTAGGACGCCATCAGTACCGTCTGCAGAATCTTGCACTTCAGTTTGGACTTACGGTACACGCAGCACAC
AGAGCAGAGGATGACGCTCGAGTATGCATGGAGCTTTTTACAACTATGATCGCACACCACGCCAAACAAAACGGACACTG
TGTAAATCATGCACAAAGCCCCACCATAAAAAAATTAATACAGGAGATACAGGCATCGAGCACTGACTGTTCCCAGGAGC
TGTTTTAG

Upstream 100 bases:

>100_bases
TCATCCACCCTCTGACCGTCAGTACCTCGATAGAAGAAGCAGAACAGGCGGGCAACATGTTTATCACCGCAGTTGAACAA
GAGATGGGTACATATCTGCA

Downstream 100 bases:

>100_bases
TATACGCTAAGCATGCGGTGCAAACGCTGTGTACCGATGACTTTCATGAAACCCGCAAGCCGTGGCCCCTGATTCTTTCC
GATCAGTACCTGGTATAGTG

Product: DNA polymerase III, subunit epsilon

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MIYDWVFAVHEHVAFTAFDTETTGLKAEEDRIIEIGAVTFDRKGIIARFSTLIFPDRAIPPDVSKINHITDDMLVNKPRF
CEIVSDFSRFIKGTVLVAHNANFDVEFLNAELSLCKKQPLSHKVVDTYAMAQAVFPGLGRHQYRLQNLALQFGLTVHAAH
RAEDDARVCMELFTTMIAHHAKQNGHCVNHAQSPTIKKLIQEIQASSTDCSQELF

Sequences:

>Translated_215_residues
MIYDWVFAVHEHVAFTAFDTETTGLKAEEDRIIEIGAVTFDRKGIIARFSTLIFPDRAIPPDVSKINHITDDMLVNKPRF
CEIVSDFSRFIKGTVLVAHNANFDVEFLNAELSLCKKQPLSHKVVDTYAMAQAVFPGLGRHQYRLQNLALQFGLTVHAAH
RAEDDARVCMELFTTMIAHHAKQNGHCVNHAQSPTIKKLIQEIQASSTDCSQELF
>Mature_215_residues
MIYDWVFAVHEHVAFTAFDTETTGLKAEEDRIIEIGAVTFDRKGIIARFSTLIFPDRAIPPDVSKINHITDDMLVNKPRF
CEIVSDFSRFIKGTVLVAHNANFDVEFLNAELSLCKKQPLSHKVVDTYAMAQAVFPGLGRHQYRLQNLALQFGLTVHAAH
RAEDDARVCMELFTTMIAHHAKQNGHCVNHAQSPTIKKLIQEIQASSTDCSQELF

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786409, Length=201, Percent_Identity=30.8457711442786, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DPO3E_TREPA (O83649)

Other databases:

- EMBL:   AE000520
- PIR:   B71299
- RefSeq:   NP_219080.1
- ProteinModelPortal:   O83649
- SMR:   O83649
- IntAct:   O83649
- GeneID:   2611776
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0643
- NMPDR:   fig|243276.1.peg.639
- TIGR:   TP_0643
- HOGENOM:   HBG251021
- OMA:   PRIRIPA
- ProtClustDB:   CLSK218802
- BioCyc:   TPAL243276:TP_0643-MONOMER
- BRENDA:   2.7.7.7
- GO:   GO:0005622
- InterPro:   IPR006054
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR012337
- SMART:   SM00479
- TIGRFAMs:   TIGR00573

Pfam domain/function: PF00929 Exonuc_X-T; SSF53098 RNaseH_fold

EC number: =2.7.7.7

Molecular weight: Translated: 24239; Mature: 24239

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: ACT_SITE 160-160 BINDING 19-19 BINDING 21-21 BINDING 62-62 BINDING 67-67 BINDING 165-165

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYDWVFAVHEHVAFTAFDTETTGLKAEEDRIIEIGAVTFDRKGIIARFSTLIFPDRAIP
CCHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCC
PDVSKINHITDDMLVNKPRFCEIVSDFSRFIKGTVLVAHNANFDVEFLNAELSLCKKQPL
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEECCCCCEEEHHHHHHHHHCCCC
SHKVVDTYAMAQAVFPGLGRHQYRLQNLALQFGLTVHAAHRAEDDARVCMELFTTMIAHH
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEHHCCCHHHHHHHHHHHHHHHHH
AKQNGHCVNHAQSPTIKKLIQEIQASSTDCSQELF
HCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure
MIYDWVFAVHEHVAFTAFDTETTGLKAEEDRIIEIGAVTFDRKGIIARFSTLIFPDRAIP
CCHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCC
PDVSKINHITDDMLVNKPRFCEIVSDFSRFIKGTVLVAHNANFDVEFLNAELSLCKKQPL
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEECCCCCEEEHHHHHHHHHCCCC
SHKVVDTYAMAQAVFPGLGRHQYRLQNLALQFGLTVHAAHRAEDDARVCMELFTTMIAHH
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEHHCCCHHHHHHHHHHHHHHHHH
AKQNGHCVNHAQSPTIKKLIQEIQASSTDCSQELF
HCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876