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Identifier: 189025860

GI number: 189025860

Start: 693835

End: 696306

Strand: Direct

Name: lig

Synonym: TPASS_0634

Alternate gene names: 189025860

Gene position: 693835-696306 (Clockwise)

Preceding gene: 189025859

Following gene: 189025864

Centisome position: 60.89

GC content: 51.17

Gene sequence:

>2472_bases
ATGAGTACTGCGCAGAGACGTGTTCAAGAACTTGAAAAGCTCATTCTTCACCATCAAGACCGCTACTACAACGCGGAGTC
TGATATTTCAGATGACGCCTTTGACGCGCTTTGGGAAGAACTTGCAAGGCTTGATCCTGGAAATCCGCTGCTTAAAGCAA
TCGGTTCTGACAGTCAACGTGACGCAGTGAAAAAGCGCCACATTGTTCCTATGGGTAGTCAACACAAAGCTGCAGACGAG
GAGTCCTTCAGCGCATGGGCAAAAAAAAATGCATTGCAGGCATTTCTCGTCCAACACAAACTCGATGGCGTTAGCTTAGA
GTTGCAATACGAGCGCGGTCATTTTTGCTGTGCGCTCACCCGTGGTAACGGCATTGTCGGAGACGACGTCACTGCTAACG
TACGCGGCATGCGTGGCTTTGTGCCGACCCTCACTGCAGAGTGGGGACCTTGCGGCAATCTCCCCTTCACGGGGGGTGTT
CGTGGCGAAGTCATTATGCACAAGGATATACATCGTTCCCACTATCCTACTCACGCAAATTGCCGCAACACCGTAAACGG
CATCCTAAAGCGAAAGGACGGCCGCGGCCGCACCCACCTCCATATTGTTTGTTACGACGCAGTACCCGGCACCCCCGGTA
AACCGTTCACCGGCTCCCTCCCCTTTGCTGATGAAACAGAAAAGCTTGCCTGGCTCGCGCGCCAAGGTTTCGTGACGGTA
CATTCGCATCGCTGCGCTAACGCACAGGAAGTTGTTGCACTCCGATCTGAGATTATGCGCACGCGCGAGCTGTTGCCTTA
CAGCATCGATGGCCTGGTAGTAAAGAGTACCGATCTTGACTTCCAGGACGCACAACTTCCCAGACCTAAAAAGCAGATAG
CCTTTAAGTTCAGTACACAGGAGGCGATAACCACCTTGCGTGACGTTCAATGGCAAACATCAGGAGTGACGTACACGCCC
ATTGGTATTACCGATCCAGTGCGCCTTGCAGGAACTACGGTCAAGCGCGCAAATTTATGTAACCCGAATATGCTCACAAA
GCTGTGTCTCAAAATTGGCAGCCACGTTTTAATTTCCAAACGGGGGGAGATTATCCCAAAAATTGAAGCACTCGTTTCAA
CCCCAGCACATGCGCAGGAAATCCACATACCGACACAGTGTACCAGTTGCAACACAGTGCTGGAAAACAGTGGGAGCAGG
CTTTTTTGTCCTAATGTAAACTGCCCCCTGCTCTCGCATCACCGCATTACACGGTGGATTGAGTGTTTGGAGATCAAGCA
TGTGGGGACCGAACTCATTCAGCGTCTTTTTGAAGAAAAAAAGGTACGCCGCATCCCGGACCTCTACACGCTCACCTGCG
AGGATCTCATTGAGATTGAGCACGTCGGCAATGCAACTGCAAAAAAAATACTCGAAGCTATACACCACAAAAAAGAAATA
GCGTTGCAAACGTTTATTGCCGGGTTTGGCATCGAGGGGATCGGAGAAACCATGGGAGAAAAACTTATCTGTGCCGGTTT
TGACACACTTGAAAAAGTGCTCCACGCAACAACGGAGACACTCGAGTCCATCTATCAGTTTGGAACAGAACTTGCAAAAA
GCGTGGTAACCGGTATTGCACGGGTTAAGGATGATATGTGTGAACTGCTTGATAGAGGCTTCGTGCGCATTCTTGCCAAG
CAGCAGGCAGAGTCGCCACTCCGGGGAAAAAGCTTTTGCTTCTCAGGCTCCCTCAGAAATGGAGATCGCGCGACTATACA
CCGTATACGCGCGCTCGGAGGCGTTGTGAGAACGTCAGTTACTCGAGACCTTTCGTACCTAATATTCGAAAGCCTATCAC
AGCCCTATCGCACCGCGCAAAAACTAAAAAAAGAACAGGGGGTAGCGCTAGAAATTATTTCGGAAGACGAGTTTTGCAGG
CTTCTAGATCAAGCTTCCGCATCCTGTACCCATACGGGGGAAACCGTACACCCCTTGCAAGGAAAGAGTTTTTATTTCTC
AGGTGCGTCGCGCAGTATGAATCACAAACATGCTCAAGAAAAAGTGCGCGCGTTAGGAGGGGATGTCGCATCGTCCGTAA
CAGCCCAGCTTGACTATCTGGTGTTTTACAGTCAGTCGACACGTTATCGCACCGCATGCGCGTTGGGAATCCAAATTATT
TCGGAAGAGACGCTCCACAAGCTTATTGCCACTGCCCAATCGCCTTTGCATACGGATGCACACGTGCACGCGCCATTGCA
CGGCATGAGCTTCTGCTTCTCTGGCGATCTCGACGGTATGACACGCGCCCAGGCAATCGCGCTCGTACAACGCCTAGGCG
GAACAGTGAAAACGGCTGTCAGTACACAGCTGACGTATCTGGTGTCAAACGATCCACATGGGCAGTCACGCAAATGCCAG
AACGCTGTTCGGTGTGGCGTGCGTATTATCAGTGAGCACGTCTTTCTCGCTCTGTGCACCCCGGGGACCTAA

Upstream 100 bases:

>100_bases
CAGGGGACTGCGCCGCCGTTTTGGCATCTTCCGACAAGACGAAGGAGCGTTGCACGCGTGGGAGGTGCGCCCCGGACGCT
ACACATTGCGGCCTTGTGCT

Downstream 100 bases:

>100_bases
CTGGACTGCACCCTGCGGTATGTTTCGGACAGTCTCATAGCACTGCGTGCGCATGTTCGAGTGTTTTTAACTTTTTACCG
CTCATTTTCGTGATCAGGCA

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 823; Mature: 822

Protein sequence:

>823_residues
MSTAQRRVQELEKLILHHQDRYYNAESDISDDAFDALWEELARLDPGNPLLKAIGSDSQRDAVKKRHIVPMGSQHKAADE
ESFSAWAKKNALQAFLVQHKLDGVSLELQYERGHFCCALTRGNGIVGDDVTANVRGMRGFVPTLTAEWGPCGNLPFTGGV
RGEVIMHKDIHRSHYPTHANCRNTVNGILKRKDGRGRTHLHIVCYDAVPGTPGKPFTGSLPFADETEKLAWLARQGFVTV
HSHRCANAQEVVALRSEIMRTRELLPYSIDGLVVKSTDLDFQDAQLPRPKKQIAFKFSTQEAITTLRDVQWQTSGVTYTP
IGITDPVRLAGTTVKRANLCNPNMLTKLCLKIGSHVLISKRGEIIPKIEALVSTPAHAQEIHIPTQCTSCNTVLENSGSR
LFCPNVNCPLLSHHRITRWIECLEIKHVGTELIQRLFEEKKVRRIPDLYTLTCEDLIEIEHVGNATAKKILEAIHHKKEI
ALQTFIAGFGIEGIGETMGEKLICAGFDTLEKVLHATTETLESIYQFGTELAKSVVTGIARVKDDMCELLDRGFVRILAK
QQAESPLRGKSFCFSGSLRNGDRATIHRIRALGGVVRTSVTRDLSYLIFESLSQPYRTAQKLKKEQGVALEIISEDEFCR
LLDQASASCTHTGETVHPLQGKSFYFSGASRSMNHKHAQEKVRALGGDVASSVTAQLDYLVFYSQSTRYRTACALGIQII
SEETLHKLIATAQSPLHTDAHVHAPLHGMSFCFSGDLDGMTRAQAIALVQRLGGTVKTAVSTQLTYLVSNDPHGQSRKCQ
NAVRCGVRIISEHVFLALCTPGT

Sequences:

>Translated_823_residues
MSTAQRRVQELEKLILHHQDRYYNAESDISDDAFDALWEELARLDPGNPLLKAIGSDSQRDAVKKRHIVPMGSQHKAADE
ESFSAWAKKNALQAFLVQHKLDGVSLELQYERGHFCCALTRGNGIVGDDVTANVRGMRGFVPTLTAEWGPCGNLPFTGGV
RGEVIMHKDIHRSHYPTHANCRNTVNGILKRKDGRGRTHLHIVCYDAVPGTPGKPFTGSLPFADETEKLAWLARQGFVTV
HSHRCANAQEVVALRSEIMRTRELLPYSIDGLVVKSTDLDFQDAQLPRPKKQIAFKFSTQEAITTLRDVQWQTSGVTYTP
IGITDPVRLAGTTVKRANLCNPNMLTKLCLKIGSHVLISKRGEIIPKIEALVSTPAHAQEIHIPTQCTSCNTVLENSGSR
LFCPNVNCPLLSHHRITRWIECLEIKHVGTELIQRLFEEKKVRRIPDLYTLTCEDLIEIEHVGNATAKKILEAIHHKKEI
ALQTFIAGFGIEGIGETMGEKLICAGFDTLEKVLHATTETLESIYQFGTELAKSVVTGIARVKDDMCELLDRGFVRILAK
QQAESPLRGKSFCFSGSLRNGDRATIHRIRALGGVVRTSVTRDLSYLIFESLSQPYRTAQKLKKEQGVALEIISEDEFCR
LLDQASASCTHTGETVHPLQGKSFYFSGASRSMNHKHAQEKVRALGGDVASSVTAQLDYLVFYSQSTRYRTACALGIQII
SEETLHKLIATAQSPLHTDAHVHAPLHGMSFCFSGDLDGMTRAQAIALVQRLGGTVKTAVSTQLTYLVSNDPHGQSRKCQ
NAVRCGVRIISEHVFLALCTPGT
>Mature_822_residues
STAQRRVQELEKLILHHQDRYYNAESDISDDAFDALWEELARLDPGNPLLKAIGSDSQRDAVKKRHIVPMGSQHKAADEE
SFSAWAKKNALQAFLVQHKLDGVSLELQYERGHFCCALTRGNGIVGDDVTANVRGMRGFVPTLTAEWGPCGNLPFTGGVR
GEVIMHKDIHRSHYPTHANCRNTVNGILKRKDGRGRTHLHIVCYDAVPGTPGKPFTGSLPFADETEKLAWLARQGFVTVH
SHRCANAQEVVALRSEIMRTRELLPYSIDGLVVKSTDLDFQDAQLPRPKKQIAFKFSTQEAITTLRDVQWQTSGVTYTPI
GITDPVRLAGTTVKRANLCNPNMLTKLCLKIGSHVLISKRGEIIPKIEALVSTPAHAQEIHIPTQCTSCNTVLENSGSRL
FCPNVNCPLLSHHRITRWIECLEIKHVGTELIQRLFEEKKVRRIPDLYTLTCEDLIEIEHVGNATAKKILEAIHHKKEIA
LQTFIAGFGIEGIGETMGEKLICAGFDTLEKVLHATTETLESIYQFGTELAKSVVTGIARVKDDMCELLDRGFVRILAKQ
QAESPLRGKSFCFSGSLRNGDRATIHRIRALGGVVRTSVTRDLSYLIFESLSQPYRTAQKLKKEQGVALEIISEDEFCRL
LDQASASCTHTGETVHPLQGKSFYFSGASRSMNHKHAQEKVRALGGDVASSVTAQLDYLVFYSQSTRYRTACALGIQIIS
EETLHKLIATAQSPLHTDAHVHAPLHGMSFCFSGDLDGMTRAQAIALVQRLGGTVKTAVSTQLTYLVSNDPHGQSRKCQN
AVRCGVRIISEHVFLALCTPGT

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 BRCT domains

Homologues:

Organism=Escherichia coli, GI1788750, Length=690, Percent_Identity=29.5652173913043, Blast_Score=239, Evalue=5e-64,
Organism=Escherichia coli, GI87082305, Length=381, Percent_Identity=24.6719160104987, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_TREPA (O83642)

Other databases:

- EMBL:   AE000520
- PIR:   H71300
- RefSeq:   NP_219072.1
- ProteinModelPortal:   O83642
- IntAct:   O83642
- GeneID:   2611515
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0634
- NMPDR:   fig|243276.1.peg.631
- TIGR:   TP_0634
- HOGENOM:   HBG251017
- OMA:   FHELKAL
- ProtClustDB:   CLSK218798
- BioCyc:   TPAL243276:TP_0634-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 91099; Mature: 90968

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 100-100 BINDING 121-121 BINDING 163-163 BINDING 275-275 BINDING 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAQRRVQELEKLILHHQDRYYNAESDISDDAFDALWEELARLDPGNPLLKAIGSDSQR
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCHHH
DAVKKRHIVPMGSQHKAADEESFSAWAKKNALQAFLVQHKLDGVSLELQYERGHFCCALT
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEE
RGNGIVGDDVTANVRGMRGFVPTLTAEWGPCGNLPFTGGVRGEVIMHKDIHRSHYPTHAN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHCCCCCCCC
CRNTVNGILKRKDGRGRTHLHIVCYDAVPGTPGKPFTGSLPFADETEKLAWLARQGFVTV
HHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEE
HSHRCANAQEVVALRSEIMRTRELLPYSIDGLVVKSTDLDFQDAQLPRPKKQIAFKFSTQ
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHEEEEECHH
EAITTLRDVQWQTSGVTYTPIGITDPVRLAGTTVKRANLCNPNMLTKLCLKIGSHVLISK
HHHHHHHHCCEECCCEEEECCCCCCCHHHCCCCCHHCCCCCHHHHHHHHHHHCCEEEEEC
RGEIIPKIEALVSTPAHAQEIHIPTQCTSCNTVLENSGSRLFCPNVNCPLLSHHRITRWI
CCCCCHHHHHHHCCCCCHHEEECCCCCCCCHHHHHCCCCEEECCCCCCCCCCHHHHHHHH
ECLEIKHVGTELIQRLFEEKKVRRIPDLYTLTCEDLIEIEHVGNATAKKILEAIHHKKEI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ALQTFIAGFGIEGIGETMGEKLICAGFDTLEKVLHATTETLESIYQFGTELAKSVVTGIA
HHHHHHHHCCCCHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVKDDMCELLDRGFVRILAKQQAESPLRGKSFCFSGSLRNGDRATIHRIRALGGVVRTSV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHH
TRDLSYLIFESLSQPYRTAQKLKKEQGVALEIISEDEFCRLLDQASASCTHTGETVHPLQ
HHHHHHHHHHHHCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCCEECCCC
GKSFYFSGASRSMNHKHAQEKVRALGGDVASSVTAQLDYLVFYSQSTRYRTACALGIQII
CCEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHH
SEETLHKLIATAQSPLHTDAHVHAPLHGMSFCFSGDLDGMTRAQAIALVQRLGGTVKTAV
HHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHCCHHHHHH
STQLTYLVSNDPHGQSRKCQNAVRCGVRIISEHVFLALCTPGT
HHEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCC
>Mature Secondary Structure 
STAQRRVQELEKLILHHQDRYYNAESDISDDAFDALWEELARLDPGNPLLKAIGSDSQR
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCHHH
DAVKKRHIVPMGSQHKAADEESFSAWAKKNALQAFLVQHKLDGVSLELQYERGHFCCALT
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEE
RGNGIVGDDVTANVRGMRGFVPTLTAEWGPCGNLPFTGGVRGEVIMHKDIHRSHYPTHAN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHCCCCCCCC
CRNTVNGILKRKDGRGRTHLHIVCYDAVPGTPGKPFTGSLPFADETEKLAWLARQGFVTV
HHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEE
HSHRCANAQEVVALRSEIMRTRELLPYSIDGLVVKSTDLDFQDAQLPRPKKQIAFKFSTQ
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHEEEEECHH
EAITTLRDVQWQTSGVTYTPIGITDPVRLAGTTVKRANLCNPNMLTKLCLKIGSHVLISK
HHHHHHHHCCEECCCEEEECCCCCCCHHHCCCCCHHCCCCCHHHHHHHHHHHCCEEEEEC
RGEIIPKIEALVSTPAHAQEIHIPTQCTSCNTVLENSGSRLFCPNVNCPLLSHHRITRWI
CCCCCHHHHHHHCCCCCHHEEECCCCCCCCHHHHHCCCCEEECCCCCCCCCCHHHHHHHH
ECLEIKHVGTELIQRLFEEKKVRRIPDLYTLTCEDLIEIEHVGNATAKKILEAIHHKKEI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ALQTFIAGFGIEGIGETMGEKLICAGFDTLEKVLHATTETLESIYQFGTELAKSVVTGIA
HHHHHHHHCCCCHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVKDDMCELLDRGFVRILAKQQAESPLRGKSFCFSGSLRNGDRATIHRIRALGGVVRTSV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHH
TRDLSYLIFESLSQPYRTAQKLKKEQGVALEIISEDEFCRLLDQASASCTHTGETVHPLQ
HHHHHHHHHHHHCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCCEECCCC
GKSFYFSGASRSMNHKHAQEKVRALGGDVASSVTAQLDYLVFYSQSTRYRTACALGIQII
CCEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHH
SEETLHKLIATAQSPLHTDAHVHAPLHGMSFCFSGDLDGMTRAQAIALVQRLGGTVKTAV
HHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHCCHHHHHH
STQLTYLVSNDPHGQSRKCQNAVRCGVRIISEHVFLALCTPGT
HHEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876