Click here to switch to the map view.

The map label for this gene is dniR [H]

Identifier: 189025849

GI number: 189025849

Start: 677933

End: 680272

Strand: Direct

Name: dniR [H]

Synonym: TPASS_0623

Alternate gene names: 189025849

Gene position: 677933-680272 (Clockwise)

Preceding gene: 189025842

Following gene: 189025850

Centisome position: 59.5

GC content: 60.13

Gene sequence:

>2340_bases
GTGCGTGCGCGCGTCAGGCGTTCTCGGCAATCTGCGTGTACCTCATCAGGAAGGCGTTTTTTCAACAGAGTCAGCGGGGG
AATGATATCCGAAAAAAAATCCGGTTCTCCAGGAATGAGCGGATACGTACCTTTTTCCACGTCATCGAGCGCAGTCAGGT
ACGGATGCGCGGTGTTGTACAGCGGCACATGCCAAGAAACAGCTTCCTGCGGATATACAAGTTGCATAAGCGCAGCACAC
ACGCGGAGGTACAGCCGATTGTGCGGTGTCAATCCGGCCGGATCGCGCTGTATGCACGCAGCTGCCTCGCGCAATGAGGC
AGGAGAAGCAGTTTCAATCAAGAAGAGTATCTTTGGATCCATCAGCTTTGTGCGAATTGCGCGTCGGTCCGGTACGTCAA
GCGCAGTGCCACGCACAGGATGCGCCGCCACCTCCGGCGCCTGGGATGCGGAAGAAACCGGATCGGTACCTGCGCGCGTC
GGCACCACCTTCGAAGAACTGCGACAACAGAGAAAAACGGGTATCAGCACAAAACCGACACTCACCCCGATGGCGCGGTC
AATGCTTTTAGTAAGCGCCCCCATAGAAACCCGTTTTTACCTCCCTGCATGGCAGTCGTGCCATTTACACAAAACGCCTG
TTGTGACCGTACCGACAACGTACGCATACCCGGCGCACCGCCGCTTCTTTTCCCTTTACGCGGTGTTCAACGCGCACGGC
GCATCCCTTGTGCTCCCCACGCAAGACATGCTAGGCTGGCTGCCACCGAGGGCGAAGAGAGCGTACAGGAGGTTAACGGT
TTTTTTGCGAGAAATCATTACCGCCCGTGCGTGTTCACTCTTCCTGTTTCTCCTTCGTTGTTTCCCTGCTGGTCCCTGTG
CGCGGGCGCGCCGGTTGTCCTTCCTTCTGTTTCTCTGTGGTGCGGCAGCCTGCCCTCCGCTTTGGGGGGCGTACGCAGCG
CACCACGCGTTGCGCGCTCAGTCGGTACCTGACACCCTCATTCAGCGCGCGCTCGTGCTCGGTCCGCTCGTGCACCCCCT
GTACCCGCCGATGCAGTCCTTCAAAGAACAGTACCGGAGCGCGCGTTACCGGGAATACCTCTCTGTCGTTATGCAGCGGA
GCGCGCCCTACCGCCCCTTTATCGAAAAACTGCTGCGCGACGCTCACCTTCCTGTCGAGCTGCTCTTTCTCCCCGTTGTC
GAATCGGGCTTTCTCGAACGGGCTGTCTCCAAATCCGGCGCAGTCGGCATTTGGCAGTTCATGCGCAATAGCATCGCAGG
ATCTGCCATGCGCGTGAGTGACTGGGTAGACGAACGGCGTGACCCCTGGAAGGCTTCCGTCGCCGCAGTCAAAAAACTGC
AGTGGAATTACACGCAGCTGCGTGACTGGCCCTTGGCCCTCGCTGCGTACAACTGCGGTCTTGGCGCGATCAAGCGAGCC
ATTGCCCAGGCAGGAACCGCCGATTTTTGGCATCTGAGTGAGCGCGGCTTTCTGCGCGACGAGACAGTCCGCTATGTCCC
AAAGTTCCTTGCGGTTGCAGAAGTACTCAGCCGGAGCCACGAGCACGGCATCGCCTGGGGAGCGGCACACACCCCCGAGG
AGACCACCACGGTTACCGTTTCGCGCGCGGTAGACTTAAACCTCTTGGCACAGGCAGCAGGGATGGATGCGCAATTGTTG
CACACGCTCAACCCTGCACTCCGCTATTCAATTACTCCCCCGAATGCAGCGTACACCCTCCGGGTGCCAAGCACACATGC
GCAGGCGGTCCAGGCAGTACTCAACCGCCCAGGCGCCGTGCTCGTCCATCACACCCTGCACACCATCCGTTCAGGAGACA
CGCTTTACGCACTCGCCCGCCGCTATGGGTTGGGCGTCGATACGCTAAAAGCCCATAACCGCGCACACAGCGCCACTCAC
CTGAAGATTGGACAAAAGCTCATCATTCCCACCATCCGGACTGGGCAGACCCGGCGCGCGCCCCTGCGGGGGGCAGGCAC
GCGTGCACCTGGAGTAATGGATCAGAAAGAGCATGCAGTGCCGGAAGTGTCCGCACTTCCCTCGTCCTCAATGACGCCGC
CGACGCCCGGGGGCGCGGACACGCGCGCGGGAGCAGACGCGCCGGCACCCCCGAGCGTGCCACCACCGCCGGAAAGCACA
GCGGCGCGGGCAACCCCTGCAGCACCCTTTGTCGGCAAGCATGTAGTGCAGCAGGGGGATACCCTCTGGTCCTTAGCAAA
GCGCTACGGCGTTTCAGTAGAAAACCTGGCAGAGGAAAACAATCTAGCAGTAGACGCCACGCTCTCCTTGGGAATGATTC
TCAAAACGCCGAGACGGTAG

Upstream 100 bases:

>100_bases
CCATGCGCTGCGGTACGCTGACTCCGCTTCAGAGAGCTCTCCGTGCTGCTCGTGCAAATACCCAAAGAGCAGGAACGGAA
GGAGAGAGTGCGGSGCCTGC

Downstream 100 bases:

>100_bases
GGTATGGGAGTGTCCTGCCTGACGCGTGCTGCCTCTTTTTCAAATCAGTGCCGTTTTCCGTTCGGCGCTCTCTCCCTGGT
GTGGACGCACGCAAGTGCGC

Product: membrane-bound lytic murein transglycosylase D

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 779; Mature: 779

Protein sequence:

>779_residues
MRARVRRSRQSACTSSGRRFFNRVSGGMISEKKSGSPGMSGYVPFSTSSSAVRYGCAVLYSGTCQETASCGYTSCISAAH
TRRYSRLCGVNPAGSRCMHAAASRNEAGEAVSIKKSIFGSISFVRIARRSGTSSAVPRTGCAATSGAWDAEETGSVPARV
GTTFEELRQQRKTGISTKPTLTPMARSMLLVSAPIETRFYLPAWQSCHLHKTPVVTVPTTYAYPAHRRFFSLYAVFNAHG
ASLVLPTQDMLGWLPPRAKRAYRRLTVFLREIITARACSLFLFLLRCFPAGPCARARRLSFLLFLCGAAACPPLWGAYAA
HHALRAQSVPDTLIQRALVLGPLVHPLYPPMQSFKEQYRSARYREYLSVVMQRSAPYRPFIEKLLRDAHLPVELLFLPVV
ESGFLERAVSKSGAVGIWQFMRNSIAGSAMRVSDWVDERRDPWKASVAAVKKLQWNYTQLRDWPLALAAYNCGLGAIKRA
IAQAGTADFWHLSERGFLRDETVRYVPKFLAVAEVLSRSHEHGIAWGAAHTPEETTTVTVSRAVDLNLLAQAAGMDAQLL
HTLNPALRYSITPPNAAYTLRVPSTHAQAVQAVLNRPGAVLVHHTLHTIRSGDTLYALARRYGLGVDTLKAHNRAHSATH
LKIGQKLIIPTIRTGQTRRAPLRGAGTRAPGVMDQKEHAVPEVSALPSSSMTPPTPGGADTRAGADAPAPPSVPPPPEST
AARATPAAPFVGKHVVQQGDTLWSLAKRYGVSVENLAEENNLAVDATLSLGMILKTPRR

Sequences:

>Translated_779_residues
MRARVRRSRQSACTSSGRRFFNRVSGGMISEKKSGSPGMSGYVPFSTSSSAVRYGCAVLYSGTCQETASCGYTSCISAAH
TRRYSRLCGVNPAGSRCMHAAASRNEAGEAVSIKKSIFGSISFVRIARRSGTSSAVPRTGCAATSGAWDAEETGSVPARV
GTTFEELRQQRKTGISTKPTLTPMARSMLLVSAPIETRFYLPAWQSCHLHKTPVVTVPTTYAYPAHRRFFSLYAVFNAHG
ASLVLPTQDMLGWLPPRAKRAYRRLTVFLREIITARACSLFLFLLRCFPAGPCARARRLSFLLFLCGAAACPPLWGAYAA
HHALRAQSVPDTLIQRALVLGPLVHPLYPPMQSFKEQYRSARYREYLSVVMQRSAPYRPFIEKLLRDAHLPVELLFLPVV
ESGFLERAVSKSGAVGIWQFMRNSIAGSAMRVSDWVDERRDPWKASVAAVKKLQWNYTQLRDWPLALAAYNCGLGAIKRA
IAQAGTADFWHLSERGFLRDETVRYVPKFLAVAEVLSRSHEHGIAWGAAHTPEETTTVTVSRAVDLNLLAQAAGMDAQLL
HTLNPALRYSITPPNAAYTLRVPSTHAQAVQAVLNRPGAVLVHHTLHTIRSGDTLYALARRYGLGVDTLKAHNRAHSATH
LKIGQKLIIPTIRTGQTRRAPLRGAGTRAPGVMDQKEHAVPEVSALPSSSMTPPTPGGADTRAGADAPAPPSVPPPPEST
AARATPAAPFVGKHVVQQGDTLWSLAKRYGVSVENLAEENNLAVDATLSLGMILKTPRR
>Mature_779_residues
MRARVRRSRQSACTSSGRRFFNRVSGGMISEKKSGSPGMSGYVPFSTSSSAVRYGCAVLYSGTCQETASCGYTSCISAAH
TRRYSRLCGVNPAGSRCMHAAASRNEAGEAVSIKKSIFGSISFVRIARRSGTSSAVPRTGCAATSGAWDAEETGSVPARV
GTTFEELRQQRKTGISTKPTLTPMARSMLLVSAPIETRFYLPAWQSCHLHKTPVVTVPTTYAYPAHRRFFSLYAVFNAHG
ASLVLPTQDMLGWLPPRAKRAYRRLTVFLREIITARACSLFLFLLRCFPAGPCARARRLSFLLFLCGAAACPPLWGAYAA
HHALRAQSVPDTLIQRALVLGPLVHPLYPPMQSFKEQYRSARYREYLSVVMQRSAPYRPFIEKLLRDAHLPVELLFLPVV
ESGFLERAVSKSGAVGIWQFMRNSIAGSAMRVSDWVDERRDPWKASVAAVKKLQWNYTQLRDWPLALAAYNCGLGAIKRA
IAQAGTADFWHLSERGFLRDETVRYVPKFLAVAEVLSRSHEHGIAWGAAHTPEETTTVTVSRAVDLNLLAQAAGMDAQLL
HTLNPALRYSITPPNAAYTLRVPSTHAQAVQAVLNRPGAVLVHHTLHTIRSGDTLYALARRYGLGVDTLKAHNRAHSATH
LKIGQKLIIPTIRTGQTRRAPLRGAGTRAPGVMDQKEHAVPEVSALPSSSMTPPTPGGADTRAGADAPAPPSVPPPPEST
AARATPAAPFVGKHVVQQGDTLWSLAKRYGVSVENLAEENNLAVDATLSLGMILKTPRR

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=318, Percent_Identity=31.1320754716981, Blast_Score=137, Evalue=4e-33,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 84831; Mature: 84831

Theoretical pI: Translated: 10.72; Mature: 10.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRARVRRSRQSACTSSGRRFFNRVSGGMISEKKSGSPGMSGYVPFSTSSSAVRYGCAVLY
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHEE
SGTCQETASCGYTSCISAAHTRRYSRLCGVNPAGSRCMHAAASRNEAGEAVSIKKSIFGS
CCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCC
ISFVRIARRSGTSSAVPRTGCAATSGAWDAEETGSVPARVGTTFEELRQQRKTGISTKPT
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCCCHHHCCHHHHHHHHHHCCCCCCCC
LTPMARSMLLVSAPIETRFYLPAWQSCHLHKTPVVTVPTTYAYPAHRRFFSLYAVFNAHG
CCHHHHHHEEEECCCCCEEECCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCC
ASLVLPTQDMLGWLPPRAKRAYRRLTVFLREIITARACSLFLFLLRCFPAGPCARARRLS
CEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
FLLFLCGAAACPPLWGAYAAHHALRAQSVPDTLIQRALVLGPLVHPLYPPMQSFKEQYRS
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ARYREYLSVVMQRSAPYRPFIEKLLRDAHLPVELLFLPVVESGFLERAVSKSGAVGIWQF
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
MRNSIAGSAMRVSDWVDERRDPWKASVAAVKKLQWNYTQLRDWPLALAAYNCGLGAIKRA
HHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHCCCCHHHHHHHCCHHHHHHH
IAQAGTADFWHLSERGFLRDETVRYVPKFLAVAEVLSRSHEHGIAWGAAHTPEETTTVTV
HHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEE
SRAVDLNLLAQAAGMDAQLLHTLNPALRYSITPPNAAYTLRVPSTHAQAVQAVLNRPGAV
EHHHHHHHHHHHCCCCHHHHHHCCHHHEEEECCCCCEEEEECCCHHHHHHHHHHCCCCEE
LVHHTLHTIRSGDTLYALARRYGLGVDTLKAHNRAHSATHLKIGQKLIIPTIRTGQTRRA
EEHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHCCCEEEECCCCCCCCCCC
PLRGAGTRAPGVMDQKEHAVPEVSALPSSSMTPPTPGGADTRAGADAPAPPSVPPPPEST
CCCCCCCCCCCCCCCHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AARATPAAPFVGKHVVQQGDTLWSLAKRYGVSVENLAEENNLAVDATLSLGMILKTPRR
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHCCCCEEEEEHHHCCHHHCCCCC
>Mature Secondary Structure
MRARVRRSRQSACTSSGRRFFNRVSGGMISEKKSGSPGMSGYVPFSTSSSAVRYGCAVLY
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHEE
SGTCQETASCGYTSCISAAHTRRYSRLCGVNPAGSRCMHAAASRNEAGEAVSIKKSIFGS
CCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCC
ISFVRIARRSGTSSAVPRTGCAATSGAWDAEETGSVPARVGTTFEELRQQRKTGISTKPT
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCCCHHHCCHHHHHHHHHHCCCCCCCC
LTPMARSMLLVSAPIETRFYLPAWQSCHLHKTPVVTVPTTYAYPAHRRFFSLYAVFNAHG
CCHHHHHHEEEECCCCCEEECCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCC
ASLVLPTQDMLGWLPPRAKRAYRRLTVFLREIITARACSLFLFLLRCFPAGPCARARRLS
CEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
FLLFLCGAAACPPLWGAYAAHHALRAQSVPDTLIQRALVLGPLVHPLYPPMQSFKEQYRS
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ARYREYLSVVMQRSAPYRPFIEKLLRDAHLPVELLFLPVVESGFLERAVSKSGAVGIWQF
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
MRNSIAGSAMRVSDWVDERRDPWKASVAAVKKLQWNYTQLRDWPLALAAYNCGLGAIKRA
HHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHCCCCHHHHHHHCCHHHHHHH
IAQAGTADFWHLSERGFLRDETVRYVPKFLAVAEVLSRSHEHGIAWGAAHTPEETTTVTV
HHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEE
SRAVDLNLLAQAAGMDAQLLHTLNPALRYSITPPNAAYTLRVPSTHAQAVQAVLNRPGAV
EHHHHHHHHHHHCCCCHHHHHHCCHHHEEEECCCCCEEEEECCCHHHHHHHHHHCCCCEE
LVHHTLHTIRSGDTLYALARRYGLGVDTLKAHNRAHSATHLKIGQKLIIPTIRTGQTRRA
EEHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHCCCEEEECCCCCCCCCCC
PLRGAGTRAPGVMDQKEHAVPEVSALPSSSMTPPTPGGADTRAGADAPAPPSVPPPPEST
CCCCCCCCCCCCCCCHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AARATPAAPFVGKHVVQQGDTLWSLAKRYGVSVENLAEENNLAVDATLSLGMILKTPRR
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHCCCCEEEEEHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]