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The map label for this gene is thiI [H]

Identifier: 189025787

GI number: 189025787

Start: 606586

End: 607791

Strand: Direct

Name: thiI [H]

Synonym: TPASS_0559

Alternate gene names: 189025787

Gene position: 606586-607791 (Clockwise)

Preceding gene: 189025786

Following gene: 189025790

Centisome position: 53.23

GC content: 55.39

Gene sequence:

>1206_bases
GTGGTGAACGGAAGTACGCAGTATCTTGCACACGTCGGTGAGCTCAGCTTAAAGAAGGGGAACCGTAGACAGTTTGAAGT
GCAGCTTGAGCGCAACCTCACGCTCATGCTACGAAGCATAAACCCTCACGTTACTGTCCGCGCAGGCAGGCTGTATCTGT
CAGTCCCGGCCTCCTTTGAAGCACAGACCACCGCTGAGCAAGCCCTCTCGTGCCTGCTGGGAATTACCGGTTGGGCTGCT
GCTACGGCGTGCCCCAAAACTATGGAAGCGATCACACGGTGTGCACATGCTGAGGCGACGCTCGCTGCGCGCGAAGGAAA
GCGAACATTCAGAATAGAGGCGCGGCGCGCGGACAAACGCTTCTGCCGTACCTCGAGTGAGATTGCACGGGAAGTCGGCG
CGGTTATCCACCAATCAGGCGCTTTGTCCGTGGATCTCCATTATCCTGACGTGGTCATTTTCATAGAAGTGCGCGAGCGC
GAAGCCTTTCTGTATGGTGCCCGACGTCGCGGCCTGCGTGGTTTACCCTGTGGCGTCTCAGGACGCGGGCTACTCCTGTT
ATCCGGCGGCATTGACTCCCCGGTAGCCGGGTACCGAATGCTTTCTCGTGGCATGCACATTGACTGTCTGTATTTCCACT
CTTATCCCTACACCCCTCCTGAAGCACAGAAAAAGGTTGAAGACCTGGCAAAGGTATTGGCGCGCTATGGACTTAGTACC
ACGCTGACAGTCGTATCGTTGACAGACATTCAAAAACAGCTCCAAACACACGCCCCTGCCCCTTCCCTCACACTGTTGCT
TCGTATGTGCATGATGCGCATTGCAGAGCACGTAGCGCGGGAACAGCGCGCACGTTGCCTTATCACTGGAGAAAGCCTTG
CACAGGTAGCAAGTCAGACGCTTGAGAACCTAACGGTGACCAGCGCGTGCACGCATCTGCCGATATTCCGCCCGCTCATT
GGTGCAGATAAAGAAGATATTATCCGCACCGCCACAGAAATCGGTACGTACGCCATTTCTATCCGTCCGTACGAGGACTG
CTGCACACTCTTCGCACCAAAACACCCAGTGCTTCGCCCAGAGGTAGAAGAAATGCAAAAACAATACCAATCTCTGATGC
TCGGTCCACTGTTAGAAGACGCGTTCCGGACGCGCAAACGCACGCGCATATACGGAAACTATGGGGTACAGGAGTCAGGC
GAATGA

Upstream 100 bases:

>100_bases
ATCGTCTGTACTTCAGCGCTCGTAGCTTCACTCGGTTGAAAGGAGAATGTACCTCCGCTATCTAGGTGACACTGCCTGGA
TAAAACCATATACCTAACAC

Downstream 100 bases:

>100_bases
GTACCGCTTATCTTACGCGGCAGCACCGTCCGCCCCTTCTTTACTGACGCGTTTATGCTAACCAATCAAGGATTATTTCC
ACCAGCGCTTCGGCGGTAAA

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MVNGSTQYLAHVGELSLKKGNRRQFEVQLERNLTLMLRSINPHVTVRAGRLYLSVPASFEAQTTAEQALSCLLGITGWAA
ATACPKTMEAITRCAHAEATLAAREGKRTFRIEARRADKRFCRTSSEIAREVGAVIHQSGALSVDLHYPDVVIFIEVRER
EAFLYGARRRGLRGLPCGVSGRGLLLLSGGIDSPVAGYRMLSRGMHIDCLYFHSYPYTPPEAQKKVEDLAKVLARYGLST
TLTVVSLTDIQKQLQTHAPAPSLTLLLRMCMMRIAEHVAREQRARCLITGESLAQVASQTLENLTVTSACTHLPIFRPLI
GADKEDIIRTATEIGTYAISIRPYEDCCTLFAPKHPVLRPEVEEMQKQYQSLMLGPLLEDAFRTRKRTRIYGNYGVQESG
E

Sequences:

>Translated_401_residues
MVNGSTQYLAHVGELSLKKGNRRQFEVQLERNLTLMLRSINPHVTVRAGRLYLSVPASFEAQTTAEQALSCLLGITGWAA
ATACPKTMEAITRCAHAEATLAAREGKRTFRIEARRADKRFCRTSSEIAREVGAVIHQSGALSVDLHYPDVVIFIEVRER
EAFLYGARRRGLRGLPCGVSGRGLLLLSGGIDSPVAGYRMLSRGMHIDCLYFHSYPYTPPEAQKKVEDLAKVLARYGLST
TLTVVSLTDIQKQLQTHAPAPSLTLLLRMCMMRIAEHVAREQRARCLITGESLAQVASQTLENLTVTSACTHLPIFRPLI
GADKEDIIRTATEIGTYAISIRPYEDCCTLFAPKHPVLRPEVEEMQKQYQSLMLGPLLEDAFRTRKRTRIYGNYGVQESG
E
>Mature_401_residues
MVNGSTQYLAHVGELSLKKGNRRQFEVQLERNLTLMLRSINPHVTVRAGRLYLSVPASFEAQTTAEQALSCLLGITGWAA
ATACPKTMEAITRCAHAEATLAAREGKRTFRIEARRADKRFCRTSSEIAREVGAVIHQSGALSVDLHYPDVVIFIEVRER
EAFLYGARRRGLRGLPCGVSGRGLLLLSGGIDSPVAGYRMLSRGMHIDCLYFHSYPYTPPEAQKKVEDLAKVLARYGLST
TLTVVSLTDIQKQLQTHAPAPSLTLLLRMCMMRIAEHVAREQRARCLITGESLAQVASQTLENLTVTSACTHLPIFRPLI
GADKEDIIRTATEIGTYAISIRPYEDCCTLFAPKHPVLRPEVEEMQKQYQSLMLGPLLEDAFRTRKRTRIYGNYGVQESG
E

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=283, Percent_Identity=29.3286219081272, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 44606; Mature: 44606

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNGSTQYLAHVGELSLKKGNRRQFEVQLERNLTLMLRSINPHVTVRAGRLYLSVPASFE
CCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCEEEEEEECCCCEEEEECEEEEECCCCCC
AQTTAEQALSCLLGITGWAAATACPKTMEAITRCAHAEATLAAREGKRTFRIEARRADKR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHH
FCRTSSEIAREVGAVIHQSGALSVDLHYPDVVIFIEVREREAFLYGARRRGLRGLPCGVS
HHHCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCHHHHHHHHHCCCCCCCCCCC
GRGLLLLSGGIDSPVAGYRMLSRGMHIDCLYFHSYPYTPPEAQKKVEDLAKVLARYGLST
CCEEEEEECCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCC
TLTVVSLTDIQKQLQTHAPAPSLTLLLRMCMMRIAEHVAREQRARCLITGESLAQVASQT
EEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECHHHHHHHHHH
LENLTVTSACTHLPIFRPLIGADKEDIIRTATEIGTYAISIRPYEDCCTLFAPKHPVLRP
HHHCCHHHHHHHCCHHHHHHCCCHHHHHHHHHHHCEEEEEEECHHHHHHHCCCCCCCCCC
EVEEMQKQYQSLMLGPLLEDAFRTRKRTRIYGNYGVQESGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCC
>Mature Secondary Structure
MVNGSTQYLAHVGELSLKKGNRRQFEVQLERNLTLMLRSINPHVTVRAGRLYLSVPASFE
CCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCEEEEEEECCCCEEEEECEEEEECCCCCC
AQTTAEQALSCLLGITGWAAATACPKTMEAITRCAHAEATLAAREGKRTFRIEARRADKR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHH
FCRTSSEIAREVGAVIHQSGALSVDLHYPDVVIFIEVREREAFLYGARRRGLRGLPCGVS
HHHCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCHHHHHHHHHCCCCCCCCCCC
GRGLLLLSGGIDSPVAGYRMLSRGMHIDCLYFHSYPYTPPEAQKKVEDLAKVLARYGLST
CCEEEEEECCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCC
TLTVVSLTDIQKQLQTHAPAPSLTLLLRMCMMRIAEHVAREQRARCLITGESLAQVASQT
EEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECHHHHHHHHHH
LENLTVTSACTHLPIFRPLIGADKEDIIRTATEIGTYAISIRPYEDCCTLFAPKHPVLRP
HHHCCHHHHHHHCCHHHHHHCCCHHHHHHHHHHHCEEEEEEECHHHHHHHCCCCCCCCCC
EVEEMQKQYQSLMLGPLLEDAFRTRKRTRIYGNYGVQESGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]