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The map label for this gene is gph2 [C]

Identifier: 189025782

GI number: 189025782

Start: 601631

End: 602299

Strand: Direct

Name: gph2 [C]

Synonym: TPASS_0554

Alternate gene names: 189025782

Gene position: 601631-602299 (Clockwise)

Preceding gene: 189025781

Following gene: 189025785

Centisome position: 52.8

GC content: 54.41

Gene sequence:

>669_bases
ATGCGGCCCATGACGCGCGCGTGTATATTCGATCTTGATGGAACGCTAACGAATACGCTGGGGACCATTGCCTACTTCGT
CAATATGCAGGCTGCCCATTACCATTTACCCCCAATTCCCTCTGAAAAGTTTGCGCTGTTTTTAGGAGATGGTTCGCGCG
CACTGATTCAGCGCGTGCTTGCTCATTACGGCGCTGCAGCTCAGACTATTTCTGAGGATGAATTTTTACAGCGCTACTGC
CTCGCGTATGAGGCAGACTTTCTCCAACGCTGTACTGTATATCCGGGGGTTCCTGAGATGCTTGTGGAGTTGAAACGACG
CCGCATAGAACTCGCCATTCTCTCCAACAAGCCACATTCTATCGCGCAGAAGGTAGCGTCTGCTTTTTTTGGGGACAATG
TTTTCTCAGTGGTGCTTGGCCAACGCGAAGGCGTACCCGTAAAATCAGATCCTGCTGGGCTTTTTGAGATCCTGCGTACC
CTAAACGTGGAGACGGCGGAGGCGCTTTTCGTCGGAGACACCGCCGTGGATATACGCACCGCGTCCGCAGCGCAAGTGCG
CAGCGTGGGAGTGCTCTGGGGCTTTCGAGACGAGACGGAGCTATCCCAGGCGCAAGCCCACGTGCTTATCAGGACGCCCG
CCGAGTTACTCCAGCACCTTTCTTTCTAG

Upstream 100 bases:

>100_bases
ATTTTCCACGACCCCCCTGCTGCAGGTGTGCACGCGCAGTCGATACACAGACAAGGAAAAAAATAGGGATATCTTGCTGC
TCGGCGCCCTTTTTATTACC

Downstream 100 bases:

>100_bases
ACTCGCGGGTACAAACTCAGACGGAGCGCACGACGCTCCCGGATCCCTGCAGGGCACGAGCCGCTACTTCTCTTCACGCC
CAACGCAGTTCGCCCGCAGG

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MRPMTRACIFDLDGTLTNTLGTIAYFVNMQAAHYHLPPIPSEKFALFLGDGSRALIQRVLAHYGAAAQTISEDEFLQRYC
LAYEADFLQRCTVYPGVPEMLVELKRRRIELAILSNKPHSIAQKVASAFFGDNVFSVVLGQREGVPVKSDPAGLFEILRT
LNVETAEALFVGDTAVDIRTASAAQVRSVGVLWGFRDETELSQAQAHVLIRTPAELLQHLSF

Sequences:

>Translated_222_residues
MRPMTRACIFDLDGTLTNTLGTIAYFVNMQAAHYHLPPIPSEKFALFLGDGSRALIQRVLAHYGAAAQTISEDEFLQRYC
LAYEADFLQRCTVYPGVPEMLVELKRRRIELAILSNKPHSIAQKVASAFFGDNVFSVVLGQREGVPVKSDPAGLFEILRT
LNVETAEALFVGDTAVDIRTASAAQVRSVGVLWGFRDETELSQAQAHVLIRTPAELLQHLSF
>Mature_222_residues
MRPMTRACIFDLDGTLTNTLGTIAYFVNMQAAHYHLPPIPSEKFALFLGDGSRALIQRVLAHYGAAAQTISEDEFLQRYC
LAYEADFLQRCTVYPGVPEMLVELKRRRIELAILSNKPHSIAQKVASAFFGDNVFSVVLGQREGVPVKSDPAGLFEILRT
LNVETAEALFVGDTAVDIRTASAAQVRSVGVLWGFRDETELSQAQAHVLIRTPAELLQHLSF

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=234, Percent_Identity=26.4957264957265, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR000150
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24568; Mature: 24568

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPMTRACIFDLDGTLTNTLGTIAYFVNMQAAHYHLPPIPSEKFALFLGDGSRALIQRVL
CCCCCHHHEEECCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCHHHHHHHH
AHYGAAAQTISEDEFLQRYCLAYEADFLQRCTVYPGVPEMLVELKRRRIELAILSNKPHS
HHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEECCCCHH
IAQKVASAFFGDNVFSVVLGQREGVPVKSDPAGLFEILRTLNVETAEALFVGDTAVDIRT
HHHHHHHHHHCCCHHHHEECCCCCCCCCCCCHHHHHHHHHCCCCHHHEEEECCCEEEEEC
ASAAQVRSVGVLWGFRDETELSQAQAHVLIRTPAELLQHLSF
CCHHHHHHHHEEECCCCHHHHHHHHHHEEEECHHHHHHHCCC
>Mature Secondary Structure
MRPMTRACIFDLDGTLTNTLGTIAYFVNMQAAHYHLPPIPSEKFALFLGDGSRALIQRVL
CCCCCHHHEEECCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCHHHHHHHH
AHYGAAAQTISEDEFLQRYCLAYEADFLQRCTVYPGVPEMLVELKRRRIELAILSNKPHS
HHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEECCCCHH
IAQKVASAFFGDNVFSVVLGQREGVPVKSDPAGLFEILRTLNVETAEALFVGDTAVDIRT
HHHHHHHHHHCCCHHHHEECCCCCCCCCCCCHHHHHHHHHCCCCHHHEEEECCCEEEEEC
ASAAQVRSVGVLWGFRDETELSQAQAHVLIRTPAELLQHLSF
CCHHHHHHHHEEECCCCHHHHHHHHHHEEEECHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA