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The map label for this gene is atpB1

Identifier: 189025660

GI number: 189025660

Start: 455961

End: 457253

Strand: Direct

Name: atpB1

Synonym: TPASS_0427

Alternate gene names: 189025660

Gene position: 455961-457253 (Clockwise)

Preceding gene: 189025659

Following gene: 189025661

Centisome position: 40.02

GC content: 50.89

Gene sequence:

>1293_bases
ATGTACAAGGTGTACGAGAAGATTGAGTCGATCAACGGTTCTGTCATCACGGTGCGGGCAAAGGGGGTGCATTACGGGGA
ATTGGCCCGGGTAAAAACGGCGTTTGGCGATTCGCTTGCTGAGGTGAATAAGCTTGAAGGGGATGTAGTTTCCCTGCAGG
TGTTTGCGGGAGGCCGGGGTATTTCCACCGGGAGCGAGGTGCGCTTTCTGGGGAGGGGTATGCTCGTGAGTTTCTCAGAT
TATTTATTGGGGAGAATTTTTAATGGCTCTGGCGTTCCCCGAGACGGTGGTCCGGCCTTAAAGGAAGGGAGGGTGGAAAT
TGGCGGTCCTTCGGTTAATCCGGCAAAGAGGGTTATCGCGCGGCGTATGATTCGCACGGGTATTCCCATGATTGATGTGT
TTAATACGCTGGTTGTTTCTCAGAAGTTGCCTATTTTTTCTAGCTCAGGGGAACCGTACAACGAACTGCTGGCCCGTATT
GCAATGCAAGCGGAGGTTGATGTCATCGTCCTCGGGGGTATGGGGCTGAAGCATGACGATTACCTGTATTTTAAAAGTGC
ATTGGAAGAGGCAGGAGCGTTGAGTAGGGCAGTCCTGTTCGTGCACACTGCCTCAGATCCTACCGTAGAATGTTTGATGG
TCCCTGATATGTGTCTTGCAGTTGCCGAGCAGTTTGCCTTGAAGGGTAGGGATGTGTTGGTGCTCTTAACCGACATGACT
AATTTTGCTGATGCTATGAAGGAAATTGCGATTATTCAGGAACAAGTTCCCTCGAATCGTGGGTATCCGGGTGACTTGTA
CAGCCAGCTTGCGTCGCGCTATGAAAAGGCGGTCGATTTTGATGATGCAGGGTCAGTGACTATCCTTGCCGTTACCACCA
TGCCAGGGGATGATGTTACGCATCCGGTTCCTGATAACACGGGGTACATTACTGAGGGTCAGTTTTATCTGAAGAACGGG
CGCATTGAACCTTTCGGGAGTCTATCCCGTCTTAAGCAGAACGTAAACGGGCGTACGCGTGCAGATCATCGCGCGCTTAT
GGATAATATGATTAAGTTGTATGCCGCCTATCGTGATGCGCTTGAGAAAAGATCTATGGGTTTTGCCATGAGCGAGTGGG
ATGAGAAACTGCTCACATACGGTGGGCTGTTTGAAGCGCAGCTCATGGATCTTTCGGTGAATATTCCGCTTGAAGAGGCG
TTCGATACGGGGTGGAAAATTCTGGGCCGGTGTTTCACCCCAGAGGAGACGGGGATTCGCTCTGATTTGATTAACACGTA
TTGGCCTGCGTAG

Upstream 100 bases:

>100_bases
GTTTGTGGAGAAAGAAAAGGAGATACGTGCCTTTCTCCAGGGAGAATCGACGAAAATCGACAGCGAAGCAGAGGGAATTA
TACGGGGGATGGAGTAGACG

Downstream 100 bases:

>100_bases
GGTGGTGACCGATGGCGGTGCGCTTAACCAAAAATGAACTGAAACGTCAAAAAGAGACGCTCAAGACGTTTCGCCGTTTT
CTCCCTACGCTTCAGCTTAA

Product: V-type ATP synthase subunit B

Products: ADP; phosphate; H+

Alternate protein names: V-ATPase subunit B 1

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MYKVYEKIESINGSVITVRAKGVHYGELARVKTAFGDSLAEVNKLEGDVVSLQVFAGGRGISTGSEVRFLGRGMLVSFSD
YLLGRIFNGSGVPRDGGPALKEGRVEIGGPSVNPAKRVIARRMIRTGIPMIDVFNTLVVSQKLPIFSSSGEPYNELLARI
AMQAEVDVIVLGGMGLKHDDYLYFKSALEEAGALSRAVLFVHTASDPTVECLMVPDMCLAVAEQFALKGRDVLVLLTDMT
NFADAMKEIAIIQEQVPSNRGYPGDLYSQLASRYEKAVDFDDAGSVTILAVTTMPGDDVTHPVPDNTGYITEGQFYLKNG
RIEPFGSLSRLKQNVNGRTRADHRALMDNMIKLYAAYRDALEKRSMGFAMSEWDEKLLTYGGLFEAQLMDLSVNIPLEEA
FDTGWKILGRCFTPEETGIRSDLINTYWPA

Sequences:

>Translated_430_residues
MYKVYEKIESINGSVITVRAKGVHYGELARVKTAFGDSLAEVNKLEGDVVSLQVFAGGRGISTGSEVRFLGRGMLVSFSD
YLLGRIFNGSGVPRDGGPALKEGRVEIGGPSVNPAKRVIARRMIRTGIPMIDVFNTLVVSQKLPIFSSSGEPYNELLARI
AMQAEVDVIVLGGMGLKHDDYLYFKSALEEAGALSRAVLFVHTASDPTVECLMVPDMCLAVAEQFALKGRDVLVLLTDMT
NFADAMKEIAIIQEQVPSNRGYPGDLYSQLASRYEKAVDFDDAGSVTILAVTTMPGDDVTHPVPDNTGYITEGQFYLKNG
RIEPFGSLSRLKQNVNGRTRADHRALMDNMIKLYAAYRDALEKRSMGFAMSEWDEKLLTYGGLFEAQLMDLSVNIPLEEA
FDTGWKILGRCFTPEETGIRSDLINTYWPA
>Mature_430_residues
MYKVYEKIESINGSVITVRAKGVHYGELARVKTAFGDSLAEVNKLEGDVVSLQVFAGGRGISTGSEVRFLGRGMLVSFSD
YLLGRIFNGSGVPRDGGPALKEGRVEIGGPSVNPAKRVIARRMIRTGIPMIDVFNTLVVSQKLPIFSSSGEPYNELLARI
AMQAEVDVIVLGGMGLKHDDYLYFKSALEEAGALSRAVLFVHTASDPTVECLMVPDMCLAVAEQFALKGRDVLVLLTDMT
NFADAMKEIAIIQEQVPSNRGYPGDLYSQLASRYEKAVDFDDAGSVTILAVTTMPGDDVTHPVPDNTGYITEGQFYLKNG
RIEPFGSLSRLKQNVNGRTRADHRALMDNMIKLYAAYRDALEKRSMGFAMSEWDEKLLTYGGLFEAQLMDLSVNIPLEEA
FDTGWKILGRCFTPEETGIRSDLINTYWPA

Specific function: Produces ATP from ADP in the presence of a proton gradient across the membrane. The V-type beta chain is a regulatory subunit

COG id: COG1156

COG function: function code C; Archaeal/vacuolar-type H+-ATPase subunit B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATPase alpha/beta chains family

Homologues:

Organism=Homo sapiens, GI19913426, Length=445, Percent_Identity=35.5056179775281, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI19913428, Length=445, Percent_Identity=35.5056179775281, Blast_Score=244, Evalue=9e-65,
Organism=Homo sapiens, GI50345984, Length=291, Percent_Identity=25.4295532646048, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI4757810, Length=291, Percent_Identity=25.4295532646048, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788251, Length=270, Percent_Identity=27.037037037037, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1790172, Length=312, Percent_Identity=27.5641025641026, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790170, Length=347, Percent_Identity=23.9193083573487, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17570191, Length=461, Percent_Identity=35.3579175704989, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17510931, Length=443, Percent_Identity=35.2144469525959, Blast_Score=240, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI71988063, Length=304, Percent_Identity=25.9868421052632, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71988080, Length=304, Percent_Identity=25.9868421052632, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71988074, Length=258, Percent_Identity=24.8062015503876, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144756, Length=357, Percent_Identity=24.6498599439776, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319603, Length=438, Percent_Identity=36.5296803652968, Blast_Score=249, Evalue=6e-67,
Organism=Saccharomyces cerevisiae, GI6319370, Length=290, Percent_Identity=24.8275862068966, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6322581, Length=366, Percent_Identity=24.8633879781421, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281361666, Length=462, Percent_Identity=34.8484848484849, Blast_Score=248, Evalue=8e-66,
Organism=Drosophila melanogaster, GI24646341, Length=462, Percent_Identity=34.8484848484849, Blast_Score=248, Evalue=8e-66,
Organism=Drosophila melanogaster, GI17136796, Length=462, Percent_Identity=34.8484848484849, Blast_Score=248, Evalue=8e-66,
Organism=Drosophila melanogaster, GI24658560, Length=291, Percent_Identity=27.8350515463918, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): VATB1_TREPA (O83442)

Other databases:

- EMBL:   AE000520
- PIR:   H71325
- RefSeq:   NP_218867.1
- ProteinModelPortal:   O83442
- SMR:   O83442
- IntAct:   O83442
- GeneID:   2610928
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0427
- NMPDR:   fig|243276.1.peg.426
- TIGR:   TP_0427
- HOGENOM:   HBG565875
- OMA:   KQAMAFE
- ProtClustDB:   PRK02118
- BioCyc:   TPAL243276:TP_0427-MONOMER
- BRENDA:   3.6.3.14
- HAMAP:   MF_00310
- InterPro:   IPR000194
- InterPro:   IPR004100
- InterPro:   IPR022879

Pfam domain/function: PF00006 ATP-synt_ab; PF02874 ATP-synt_ab_N

EC number: 3.6.3.14

Molecular weight: Translated: 47230; Mature: 47230

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKVYEKIESINGSVITVRAKGVHYGELARVKTAFGDSLAEVNKLEGDVVSLQVFAGGRG
CCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHHCCCCEEEEEEEECCCC
ISTGSEVRFLGRGMLVSFSDYLLGRIFNGSGVPRDGGPALKEGRVEIGGPSVNPAKRVIA
CCCCCCCCEECCCHHHHHHHHHHHEEECCCCCCCCCCCCHHCCEEEECCCCCCHHHHHHH
RRMIRTGIPMIDVFNTLVVSQKLPIFSSSGEPYNELLARIAMQAEVDVIVLGGMGLKHDD
HHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCC
YLYFKSALEEAGALSRAVLFVHTASDPTVECLMVPDMCLAVAEQFALKGRDVLVLLTDMT
HHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEECHHHHHHHHHHHHCCCCCEEEEEECHH
NFADAMKEIAIIQEQVPSNRGYPGDLYSQLASRYEKAVDFDDAGSVTILAVTTMPGDDVT
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCC
HPVPDNTGYITEGQFYLKNGRIEPFGSLSRLKQNVNGRTRADHRALMDNMIKLYAAYRDA
CCCCCCCCEEECCEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LEKRSMGFAMSEWDEKLLTYGGLFEAQLMDLSVNIPLEEAFDTGWKILGRCFTPEETGIR
HHHHHCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHH
SDLINTYWPA
HHHHHHCCCC
>Mature Secondary Structure
MYKVYEKIESINGSVITVRAKGVHYGELARVKTAFGDSLAEVNKLEGDVVSLQVFAGGRG
CCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHHCCCCEEEEEEEECCCC
ISTGSEVRFLGRGMLVSFSDYLLGRIFNGSGVPRDGGPALKEGRVEIGGPSVNPAKRVIA
CCCCCCCCEECCCHHHHHHHHHHHEEECCCCCCCCCCCCHHCCEEEECCCCCCHHHHHHH
RRMIRTGIPMIDVFNTLVVSQKLPIFSSSGEPYNELLARIAMQAEVDVIVLGGMGLKHDD
HHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCC
YLYFKSALEEAGALSRAVLFVHTASDPTVECLMVPDMCLAVAEQFALKGRDVLVLLTDMT
HHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEECHHHHHHHHHHHHCCCCCEEEEEECHH
NFADAMKEIAIIQEQVPSNRGYPGDLYSQLASRYEKAVDFDDAGSVTILAVTTMPGDDVT
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCC
HPVPDNTGYITEGQFYLKNGRIEPFGSLSRLKQNVNGRTRADHRALMDNMIKLYAAYRDA
CCCCCCCCEEECCEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LEKRSMGFAMSEWDEKLLTYGGLFEAQLMDLSVNIPLEEAFDTGWKILGRCFTPEETGIR
HHHHHCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHH
SDLINTYWPA
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Borate; Diphosphate; HCO3- [C]

Metal ions: Co2+; Fe2+; Mn2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O; H+

Specific reaction: ATP + H2O + H+(in) = ADP + phosphate + H+(out)

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: Ca2+; CN-; Efrapeptin; Ethidiumbromide; Guanidines various reactions catalyzed by ATPase, analogs; Oligomycin; Quercetin; Trialkyl tinderivatives; Venturicidin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876