Click here to switch to the map view.

The map label for this gene is proB

Identifier: 189025584

GI number: 189025584

Start: 377102

End: 377992

Strand: Direct

Name: proB

Synonym: TPASS_0351

Alternate gene names: 189025584

Gene position: 377102-377992 (Clockwise)

Preceding gene: 189025583

Following gene: 189025585

Centisome position: 33.09

GC content: 55.89

Gene sequence:

>891_bases
GTGATCCGTGCGCTTTTTGCTGCGGCAAAAAAAATTGTGATAAAGATTGGGTCAAATACGCTTGCGCAGGCAGATGGTAC
TCCTGATGAGGAGTTTTTGGCGGAGTGTGCTCGCGCCTGTGCGGCGCTGATGCGTGACGGCAAGCAGATAGTTGTGGTGT
CGTCTGGCGCTCAGGTTGCAGGGATTTCTGCGCTCCATTGCCTTTCATCTCCTCCTCAGGGGGCGGGTTTAGAGCGTCAC
GAATCGCGCGGCGTTATTCCGGGTGATGGTGCGTCCTGCAAACAGGCGTTGTGTGCGGTGGGTCAGGCGGAGTTGATAAG
TCGTTGGCGTTCTGCGTTTGCAGCGCACCAGCAGTGCGTGGGCCAGTTTCTGTGTACGAAGGAGGATTTTACTGACTCGG
ACCGCGCGGCGCAGGTACGCTACACGTTGTCCTTTTTGCTCGAGCGCAGGGTAGTACCTATCCTTAATGAAAATGACGCG
CTCTGTTGCAGCGACGTCCCCTCTGTACCCGCCGACCGGCGGGTGTCCCTATCACCTCAAAAAAGGATTGGAGATAATGA
CAGTCTGTCCGCGTTTGTAGCGCTGTTGTGGCAGGCAGATCTTTTGCTTTTGTTGAGTGACATTGACGGCGTGTATGACA
AAGACCCAAAGGCACACACAGATGCGCAGCACGTTCCTCTGGTGACGGACGTGTCAGCGCTTGTGGGTAAAACGAGCATG
GGTTCTTCCAATGTCTTTGGTACGGGTGGGATTGCTACAAAGCTGGATGCTGCGCGTCTTGTCACGAGGGCGGGAATTCC
TCTGGTGCTGGCAAACGGGCGCCATCTGGATCCGATCCTGAGCCTTATGCGCGGGGATGCGCGGGGGACACTTTTCGTGC
CTGTTTCTTAG

Upstream 100 bases:

>100_bases
TTGGGGTCAGTACGCAAAAATTGCACGCGCGCGGTCCGATGGGTTTGTGTGCACTGACTACTTCAAAATATCTGATTGAT
GGAGAGGGGCAGGTGCGTCC

Downstream 100 bases:

>100_bases
AGAGCGACGTGGGTATGCGCAAGTGCACGCATTGTGCCCTATAATGCGCGGCGTGCGGTCAATTTCTGACGTGTAATTTT
TCTCGGTGGGGCGACGTCTC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MIRALFAAAKKIVIKIGSNTLAQADGTPDEEFLAECARACAALMRDGKQIVVVSSGAQVAGISALHCLSSPPQGAGLERH
ESRGVIPGDGASCKQALCAVGQAELISRWRSAFAAHQQCVGQFLCTKEDFTDSDRAAQVRYTLSFLLERRVVPILNENDA
LCCSDVPSVPADRRVSLSPQKRIGDNDSLSAFVALLWQADLLLLLSDIDGVYDKDPKAHTDAQHVPLVTDVSALVGKTSM
GSSNVFGTGGIATKLDAARLVTRAGIPLVLANGRHLDPILSLMRGDARGTLFVPVS

Sequences:

>Translated_296_residues
MIRALFAAAKKIVIKIGSNTLAQADGTPDEEFLAECARACAALMRDGKQIVVVSSGAQVAGISALHCLSSPPQGAGLERH
ESRGVIPGDGASCKQALCAVGQAELISRWRSAFAAHQQCVGQFLCTKEDFTDSDRAAQVRYTLSFLLERRVVPILNENDA
LCCSDVPSVPADRRVSLSPQKRIGDNDSLSAFVALLWQADLLLLLSDIDGVYDKDPKAHTDAQHVPLVTDVSALVGKTSM
GSSNVFGTGGIATKLDAARLVTRAGIPLVLANGRHLDPILSLMRGDARGTLFVPVS
>Mature_296_residues
MIRALFAAAKKIVIKIGSNTLAQADGTPDEEFLAECARACAALMRDGKQIVVVSSGAQVAGISALHCLSSPPQGAGLERH
ESRGVIPGDGASCKQALCAVGQAELISRWRSAFAAHQQCVGQFLCTKEDFTDSDRAAQVRYTLSFLLERRVVPILNENDA
LCCSDVPSVPADRRVSLSPQKRIGDNDSLSAFVALLWQADLLLLLSDIDGVYDKDPKAHTDAQHVPLVTDVSALVGKTSM
GSSNVFGTGGIATKLDAARLVTRAGIPLVLANGRHLDPILSLMRGDARGTLFVPVS

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate 5-kinase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=305, Percent_Identity=28.8524590163934, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI21361368, Length=307, Percent_Identity=28.9902280130293, Blast_Score=101, Evalue=7e-22,
Organism=Escherichia coli, GI1786437, Length=284, Percent_Identity=34.5070422535211, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17569969, Length=290, Percent_Identity=29.6551724137931, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17569967, Length=290, Percent_Identity=29.6551724137931, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321822, Length=268, Percent_Identity=34.3283582089552, Blast_Score=137, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320506, Length=258, Percent_Identity=32.9457364341085, Blast_Score=129, Evalue=8e-31,
Organism=Drosophila melanogaster, GI21357643, Length=301, Percent_Identity=31.5614617940199, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_TREPA (P74936)

Other databases:

- EMBL:   U61535
- EMBL:   AE000520
- PIR:   F71336
- RefSeq:   NP_218791.1
- ProteinModelPortal:   P74936
- SMR:   P74936
- IntAct:   P74936
- GeneID:   2611465
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0351
- NMPDR:   fig|243276.1.peg.350
- TIGR:   TP_0351
- HOGENOM:   HBG507643
- OMA:   LINTIDP
- ProtClustDB:   PRK12314
- BioCyc:   TPAL243276:TP_0351-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.2.11

Molecular weight: Translated: 31315; Mature: 31315

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRALFAAAKKIVIKIGSNTLAQADGTPDEEFLAECARACAALMRDGKQIVVVSSGAQVA
CHHHHHHHHHHEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCH
GISALHCLSSPPQGAGLERHESRGVIPGDGASCKQALCAVGQAELISRWRSAFAAHQQCV
HHHHHHHHCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
GQFLCTKEDFTDSDRAAQVRYTLSFLLERRVVPILNENDALCCSDVPSVPADRRVSLSPQ
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCEEECCCCCCCCCCEECCCHH
KRIGDNDSLSAFVALLWQADLLLLLSDIDGVYDKDPKAHTDAQHVPLVTDVSALVGKTSM
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
GSSNVFGTGGIATKLDAARLVTRAGIPLVLANGRHLDPILSLMRGDARGTLFVPVS
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure
MIRALFAAAKKIVIKIGSNTLAQADGTPDEEFLAECARACAALMRDGKQIVVVSSGAQVA
CHHHHHHHHHHEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCH
GISALHCLSSPPQGAGLERHESRGVIPGDGASCKQALCAVGQAELISRWRSAFAAHQQCV
HHHHHHHHCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
GQFLCTKEDFTDSDRAAQVRYTLSFLLERRVVPILNENDALCCSDVPSVPADRRVSLSPQ
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCEEECCCCCCCCCCEECCCHH
KRIGDNDSLSAFVALLWQADLLLLLSDIDGVYDKDPKAHTDAQHVPLVTDVSALVGKTSM
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
GSSNVFGTGGIATKLDAARLVTRAGIPLVLANGRHLDPILSLMRGDARGTLFVPVS
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9522123; 9665876