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The map label for this gene is proA

Identifier: 189025583

GI number: 189025583

Start: 375819

End: 377105

Strand: Direct

Name: proA

Synonym: TPASS_0350

Alternate gene names: 189025583

Gene position: 375819-377105 (Clockwise)

Preceding gene: 189025580

Following gene: 189025584

Centisome position: 32.98

GC content: 55.71

Gene sequence:

>1287_bases
ATGGTAGAGGTCTACGCGCGGTTGCGCGCTGCTGTTGCTCGGTTGGCGGTCTGTAGCGCTGCGGAGAAGGACGGTGCCCT
GCGCGCTGTGCGCGATGCGCTACATGCGCAGCGGGAGGATATCCTGCGTGCAAATGCGCAGGATCTTGCGCGGGCGCGTG
AGGCGGGTCTTGCCGCACCGCTTGTCGCCCGGCTCGCGCTGAGTGAACACCTTCTTGAGGACATGTTGCGGTCTTTGACT
GTTCTTTCGCTTCAGCGGGATCCTATCGGGGAAATTATAGAAGGGTACACTCTTGCGAATGGACTGGAAATCCGGAAGGT
ACGTGTTCCTCTGGGGGTGGTGGCTGTCATCTACGAGTCTCGGCCCAACGTGACCGTAGATGCGTTTGCACTTGCGTACA
AAAGCGGCAATGCGGTGCTCCTGCGCGCAGGTTCTGCAGCGAGTTATTCAAATGCCCCGCTTTTGCGCGCAATTCACGTG
GGTTTGAAGAAAGCGCATGGTGTCGTGGACGCGGTGGCTGTTCCTCCCGTTTTGGAGGAAAAATATGGTGATGTGGATCA
TATCCTCCGCGCGCGCGGCTTTATCGATGCGGTATTTCCTCGTGGGGGGGCGGCGCTTATCCGGCGCGTCGTGGAAGGCG
CCCACGTGCCAGTTATTGAAACCGGATGCGGCGTGTGCCACCTATACGTAGATGAGAGTGCGAATATCGATGTGGCGCTG
CAGATTGCAGAAAACGCGAAGTTGCAAAAACCGGCCGCATGCAATTCAGTCGAAACGCTGTTGGTGCATCGTGCGGTTGC
GCGTCCTTTTTTGCACCGTGTACAGGAGATTTTTGCCACCTGTGAGGAGACTACGCGCAAGCCCGGTGGTGTGGATTTTT
TTTGTGATGCTGAGTCTTTCTCCCTTCTCACAGAAAGGGGCGCGAGAAAAAATGTTTTTCATGCACAGGCAGAGACCTGG
GATCGGGAATACCTGGACTATCAGGTATCCGTGCGGGTGGTGCCAAACCTTGAAGAAGCACTCAGGCACATTGCTCGTCA
TTCTACGAAACACTCAGAGGTTATTGTCACGCGCGATCGTGCCCGTGCGCGTCGTTTTCATCAGGAAGTAGATGCTGCCT
GTGTATATGTCAATGCTTCAAGTAGGTTTACCGATGGAGGGCAGTTTGGCATGGGAGCAGAGATTGGGGTCAGTACGCAA
AAATTGCACGCGCGCGGTCCGATGGGTTTGTGTGCACTGACTACTTCAAAATATCTGATTGATGGAGAGGGGCAGGTGCG
TCCGTGA

Upstream 100 bases:

>100_bases
TGGTCCACTTCTTGCCCTCTTGCGCGGTGCTGTGCGGTAAAAGAGGGGGCGTCGCGTTCGAGTAAAATTTTCTCTTAAGC
CTTAAGTGAGATACCCCATT

Downstream 100 bases:

>100_bases
TCCGTGCGCTTTTTGCTGCGGCAAAAAAAATTGTGATAAAGATTGGGTCAAATACGCTTGCGCAGGCAGATGGTACTCCT
GATGAGGAGTTTTTGGCGGA

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MVEVYARLRAAVARLAVCSAAEKDGALRAVRDALHAQREDILRANAQDLARAREAGLAAPLVARLALSEHLLEDMLRSLT
VLSLQRDPIGEIIEGYTLANGLEIRKVRVPLGVVAVIYESRPNVTVDAFALAYKSGNAVLLRAGSAASYSNAPLLRAIHV
GLKKAHGVVDAVAVPPVLEEKYGDVDHILRARGFIDAVFPRGGAALIRRVVEGAHVPVIETGCGVCHLYVDESANIDVAL
QIAENAKLQKPAACNSVETLLVHRAVARPFLHRVQEIFATCEETTRKPGGVDFFCDAESFSLLTERGARKNVFHAQAETW
DREYLDYQVSVRVVPNLEEALRHIARHSTKHSEVIVTRDRARARRFHQEVDAACVYVNASSRFTDGGQFGMGAEIGVSTQ
KLHARGPMGLCALTTSKYLIDGEGQVRP

Sequences:

>Translated_428_residues
MVEVYARLRAAVARLAVCSAAEKDGALRAVRDALHAQREDILRANAQDLARAREAGLAAPLVARLALSEHLLEDMLRSLT
VLSLQRDPIGEIIEGYTLANGLEIRKVRVPLGVVAVIYESRPNVTVDAFALAYKSGNAVLLRAGSAASYSNAPLLRAIHV
GLKKAHGVVDAVAVPPVLEEKYGDVDHILRARGFIDAVFPRGGAALIRRVVEGAHVPVIETGCGVCHLYVDESANIDVAL
QIAENAKLQKPAACNSVETLLVHRAVARPFLHRVQEIFATCEETTRKPGGVDFFCDAESFSLLTERGARKNVFHAQAETW
DREYLDYQVSVRVVPNLEEALRHIARHSTKHSEVIVTRDRARARRFHQEVDAACVYVNASSRFTDGGQFGMGAEIGVSTQ
KLHARGPMGLCALTTSKYLIDGEGQVRP
>Mature_428_residues
MVEVYARLRAAVARLAVCSAAEKDGALRAVRDALHAQREDILRANAQDLARAREAGLAAPLVARLALSEHLLEDMLRSLT
VLSLQRDPIGEIIEGYTLANGLEIRKVRVPLGVVAVIYESRPNVTVDAFALAYKSGNAVLLRAGSAASYSNAPLLRAIHV
GLKKAHGVVDAVAVPPVLEEKYGDVDHILRARGFIDAVFPRGGAALIRRVVEGAHVPVIETGCGVCHLYVDESANIDVAL
QIAENAKLQKPAACNSVETLLVHRAVARPFLHRVQEIFATCEETTRKPGGVDFFCDAESFSLLTERGARKNVFHAQAETW
DREYLDYQVSVRVVPNLEEALRHIARHSTKHSEVIVTRDRARARRFHQEVDAACVYVNASSRFTDGGQFGMGAEIGVSTQ
KLHARGPMGLCALTTSKYLIDGEGQVRP

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI21361368, Length=431, Percent_Identity=36.8909512761021, Blast_Score=258, Evalue=1e-68,
Organism=Homo sapiens, GI62912457, Length=431, Percent_Identity=36.8909512761021, Blast_Score=257, Evalue=1e-68,
Organism=Escherichia coli, GI1786438, Length=419, Percent_Identity=44.6300715990453, Blast_Score=326, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17569967, Length=432, Percent_Identity=34.2592592592593, Blast_Score=238, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI17569969, Length=432, Percent_Identity=34.2592592592593, Blast_Score=238, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6324899, Length=429, Percent_Identity=34.7319347319347, Blast_Score=249, Evalue=6e-67,
Organism=Drosophila melanogaster, GI21357643, Length=428, Percent_Identity=36.4485981308411, Blast_Score=254, Evalue=7e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_TREPA (P74935)

Other databases:

- EMBL:   U61535
- EMBL:   AE000520
- PIR:   E71336
- RefSeq:   NP_218790.1
- ProteinModelPortal:   P74935
- SMR:   P74935
- IntAct:   P74935
- GeneID:   2611456
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0350
- NMPDR:   fig|243276.1.peg.349
- TIGR:   TP_0350
- HOGENOM:   HBG318080
- OMA:   GICGAME
- ProtClustDB:   PRK00197
- BioCyc:   TPAL243276:TP_0350-MONOMER
- BRENDA:   1.2.1.41
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 46609; Mature: 46609

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEVYARLRAAVARLAVCSAAEKDGALRAVRDALHAQREDILRANAQDLARAREAGLAAP
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHH
LVARLALSEHLLEDMLRSLTVLSLQRDPIGEIIEGYTLANGLEIRKVRVPLGVVAVIYES
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEECCCEEEEEEEEC
RPNVTVDAFALAYKSGNAVLLRAGSAASYSNAPLLRAIHVGLKKAHGVVDAVAVPPVLEE
CCCCEEEEEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCHHHH
KYGDVDHILRARGFIDAVFPRGGAALIRRVVEGAHVPVIETGCGVCHLYVDESANIDVAL
HCCCHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCEEECCCCEEEEEECCCCCCEEEE
QIAENAKLQKPAACNSVETLLVHRAVARPFLHRVQEIFATCEETTRKPGGVDFFCDAESF
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHH
SLLTERGARKNVFHAQAETWDREYLDYQVSVRVVPNLEEALRHIARHSTKHSEVIVTRDR
HHHHHCCCCCCCEEHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHCCCCCCEEEEECCH
ARARRFHQEVDAACVYVNASSRFTDGGQFGMGAEIGVSTQKLHARGPMGLCALTTSKYLI
HHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCCEEE
DGEGQVRP
CCCCCCCC
>Mature Secondary Structure
MVEVYARLRAAVARLAVCSAAEKDGALRAVRDALHAQREDILRANAQDLARAREAGLAAP
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHH
LVARLALSEHLLEDMLRSLTVLSLQRDPIGEIIEGYTLANGLEIRKVRVPLGVVAVIYES
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEECCCEEEEEEEEC
RPNVTVDAFALAYKSGNAVLLRAGSAASYSNAPLLRAIHVGLKKAHGVVDAVAVPPVLEE
CCCCEEEEEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCHHHH
KYGDVDHILRARGFIDAVFPRGGAALIRRVVEGAHVPVIETGCGVCHLYVDESANIDVAL
HCCCHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCEEECCCCEEEEEECCCCCCEEEE
QIAENAKLQKPAACNSVETLLVHRAVARPFLHRVQEIFATCEETTRKPGGVDFFCDAESF
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHH
SLLTERGARKNVFHAQAETWDREYLDYQVSVRVVPNLEEALRHIARHSTKHSEVIVTRDR
HHHHHCCCCCCCEEHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHCCCCCCEEEEECCH
ARARRFHQEVDAACVYVNASSRFTDGGQFGMGAEIGVSTQKLHARGPMGLCALTTSKYLI
HHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCCEEE
DGEGQVRP
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9522123; 9665876