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The map label for this gene is spsF [H]

Identifier: 189025522

GI number: 189025522

Start: 302366

End: 303313

Strand: Direct

Name: spsF [H]

Synonym: TPASS_0288

Alternate gene names: 189025522

Gene position: 302366-303313 (Clockwise)

Preceding gene: 189025521

Following gene: 189025523

Centisome position: 26.54

GC content: 52.32

Gene sequence:

>948_bases
ATGGAGCAGGATCGTTCCGGTGTTGCAGTTATTGTGCAAGCACGCGTTGATTCTACGCGGTTACCAGGAAAGGCGCTGTT
GCCCCTTATGGGGGAGCCGCTGATACATTACGTGTTGCAGGCAACGCGGGTCATCCCTGCAGAAACGTATATTCTTGCGT
GTGATGAACATTCTAAAAAAGATTTTGAACCCGTTGCGCGCGCGCATGGGTTCTACTGTATTTCAGGATCGGCAGAAGAT
GTGCTCCACCGCTTTTGTATCGCAGTTAAGGCTTTTGAGCATTCGTTCCCAATTCGAACCGTCGTGCGGGTTACCGGAGA
TAACCCTTTTTTGTTTCACGAAGCAGCTGCTGCAGCGCTCCTGCGTTATGCGGAACTGGACGAGCCAGATTATTTTACCT
TCACTGGTCTTCCCTACGGCAGCGGAGTGGAAATATTAAAGGCGCGCTCGTTGCTTTTAGCAGATCGTCTTCCTTTGGAG
GCGTACGACCGTGAGCATGTAGGACCTGCCTTACATCGCCGCCCGGGTATTTTCGTGTGTGTGCGTGAACCTGCCGCTGC
CGCATGGTATCACCCTGATGTGCGAGTAACGGTGGACACGCAGGAAGACTTTCAGCGAGCGCAGCATATGATGGAGTACG
TGTGCACGTTGCAGTGTCCGTTTCCGCTCTCTTCCGATGTTATTTTAGATGCGTATGCGTACGCGCGGCAGGTGGTGGTA
TTTGTACCCTCAGTGAAAATAGGACAGGGAGCGGGGCATTTATATCGGGCGGCGTATTTAGTCTTGCGACTGCAAGGACG
TGTGCGCTGTTGCTTGTATGTGCCCGATCGTAGTTTGCTGTGCAAACTGTCTACTCCCTTTCCTGAGGAACTGATAGTGG
ACACGGTTCCTGCGGATGCATCACTGATAGTGGTCGACAACTTCCGTACTTGCTACGTGCGAGATTGA

Upstream 100 bases:

>100_bases
CGTACCGTCTCTTTCGGGACGCTCAGAAATGGGTGGCGGTGAATAGATACCGTTTTAAAAAAACTGCCGCGCGTTTAGTA
AGTGAGTGGGAACCACGTTA

Downstream 100 bases:

>100_bases
ATTACTTCAGCGTACTGCACCAGTGCTTGCTCTGGATGAGGGGGGATCTGGACGTCTGAATGCGGATTACTTGATAGATG
TATTTCCCGTCTTGCAAAGT

Product: spore coat polysaccharide biosynthesis protein F

Products: CMP-3-deoxy-D-manno-octulosonate; Diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MEQDRSGVAVIVQARVDSTRLPGKALLPLMGEPLIHYVLQATRVIPAETYILACDEHSKKDFEPVARAHGFYCISGSAED
VLHRFCIAVKAFEHSFPIRTVVRVTGDNPFLFHEAAAAALLRYAELDEPDYFTFTGLPYGSGVEILKARSLLLADRLPLE
AYDREHVGPALHRRPGIFVCVREPAAAAWYHPDVRVTVDTQEDFQRAQHMMEYVCTLQCPFPLSSDVILDAYAYARQVVV
FVPSVKIGQGAGHLYRAAYLVLRLQGRVRCCLYVPDRSLLCKLSTPFPEELIVDTVPADASLIVVDNFRTCYVRD

Sequences:

>Translated_315_residues
MEQDRSGVAVIVQARVDSTRLPGKALLPLMGEPLIHYVLQATRVIPAETYILACDEHSKKDFEPVARAHGFYCISGSAED
VLHRFCIAVKAFEHSFPIRTVVRVTGDNPFLFHEAAAAALLRYAELDEPDYFTFTGLPYGSGVEILKARSLLLADRLPLE
AYDREHVGPALHRRPGIFVCVREPAAAAWYHPDVRVTVDTQEDFQRAQHMMEYVCTLQCPFPLSSDVILDAYAYARQVVV
FVPSVKIGQGAGHLYRAAYLVLRLQGRVRCCLYVPDRSLLCKLSTPFPEELIVDTVPADASLIVVDNFRTCYVRD
>Mature_315_residues
MEQDRSGVAVIVQARVDSTRLPGKALLPLMGEPLIHYVLQATRVIPAETYILACDEHSKKDFEPVARAHGFYCISGSAED
VLHRFCIAVKAFEHSFPIRTVVRVTGDNPFLFHEAAAAALLRYAELDEPDYFTFTGLPYGSGVEILKARSLLLADRLPLE
AYDREHVGPALHRRPGIFVCVREPAAAAWYHPDVRVTVDTQEDFQRAQHMMEYVCTLQCPFPLSSDVILDAYAYARQVVV
FVPSVKIGQGAGHLYRAAYLVLRLQGRVRCCLYVPDRSLLCKLSTPFPEELIVDTVPADASLIVVDNFRTCYVRD

Specific function: Activates Kdo (A Required 8-Carbon Sugar) For Incorporation Into Bacterial Lipopolysaccharide In Gram-Negative Bacteria (By Similarity). [C]

COG id: COG1861

COG function: function code M; Spore coat polysaccharide biosynthesis protein F, CMP-KDO synthetase homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CMP-NeuNAc synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003329 [H]

Pfam domain/function: PF02348 CTP_transf_3 [H]

EC number: 2.7.7.38 [C]

Molecular weight: Translated: 35283; Mature: 35283

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQDRSGVAVIVQARVDSTRLPGKALLPLMGEPLIHYVLQATRVIPAETYILACDEHSKK
CCCCCCCEEEEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCC
DFEPVARAHGFYCISGSAEDVLHRFCIAVKAFEHSFPIRTVVRVTGDNPFLFHEAAAAAL
CHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEHHHHHHHH
LRYAELDEPDYFTFTGLPYGSGVEILKARSLLLADRLPLEAYDREHVGPALHRRPGIFVC
HHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHCCCCEEEE
VREPAAAAWYHPDVRVTVDTQEDFQRAQHMMEYVCTLQCPFPLSSDVILDAYAYARQVVV
EECCCCCEEECCCEEEEECCHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHCEEEE
FVPSVKIGQGAGHLYRAAYLVLRLQGRVRCCLYVPDRSLLCKLSTPFPEELIVDTVPADA
EECCEEECCCCCHHHHHHHHEEEECCCEEEEEEECCCCEEEEECCCCCHHHEEECCCCCC
SLIVVDNFRTCYVRD
EEEEEECCEEEEEEC
>Mature Secondary Structure
MEQDRSGVAVIVQARVDSTRLPGKALLPLMGEPLIHYVLQATRVIPAETYILACDEHSKK
CCCCCCCEEEEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCC
DFEPVARAHGFYCISGSAEDVLHRFCIAVKAFEHSFPIRTVVRVTGDNPFLFHEAAAAAL
CHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEHHHHHHHH
LRYAELDEPDYFTFTGLPYGSGVEILKARSLLLADRLPLEAYDREHVGPALHRRPGIFVC
HHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHCCCCEEEE
VREPAAAAWYHPDVRVTVDTQEDFQRAQHMMEYVCTLQCPFPLSSDVILDAYAYARQVVV
EECCCCCEEECCCEEEEECCHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHCEEEE
FVPSVKIGQGAGHLYRAAYLVLRLQGRVRCCLYVPDRSLLCKLSTPFPEELIVDTVPADA
EECCEEECCCCCHHHHHHHHEEEECCCEEEEEEECCCCEEEEECCCCCHHHEEECCCCCC
SLIVVDNFRTCYVRD
EEEEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: GSH [C]

Metal ions: Ba2+; Ca2+; Cd2+; Co2+; Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.34 {dCTP}} 0.88 {UTP}} 0.8 {3-deoxy-manno-octulosonate}} 0.39 {3-deoxy-manno-octulosonate}} 0.29 {3-deoxy-manno-octulosonate}} 0.22 {CTP}} 0.2 {CTP}} [C]

Substrates: CTP; 3-Deoxy-D-manno-2-octulosonate [C]

Specific reaction: CTP + 3-Deoxy-D-manno-2-octulosonate --> CMP-3-deoxy-D-manno-octulosonate + Diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 2,6-Anhydro-3-deoxy-D-glycero-D-talo-octanoate; Diphosphate; Hg2+; 3-deoxy-manno-octulosonate 8-phosphate; N-acetyl neuraminate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]