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The map label for this gene is braC [H]

Identifier: 189025499

GI number: 189025499

Start: 278088

End: 279455

Strand: Reverse

Name: braC [H]

Synonym: TPASS_0265

Alternate gene names: 189025499

Gene position: 279455-278088 (Counterclockwise)

Preceding gene: 189025505

Following gene: 189025494

Centisome position: 24.53

GC content: 54.9

Gene sequence:

>1368_bases
GTGATACATACTCACACGCTCTCGCTGAGCTTCATGCTGTTTTCATTCTTCTTCGGTGCAGGAAACCTCATCCTTCCCCC
CTTACTGGGAAAACACGCAGGTACGACACTCGCCACGGCGTTGCTCGGCTTTGCCACTTCCGCAGTCCTCATACCAATCG
CAGGGCTCATTACTATCGCACACGCAGGCGGTATTGTCCCTTTGTCAGAAAGGGTAGGAAAACGCTTCGCTCACTTGTAT
CCGGCTATTACTCTCCTTGTCATCGGACCGGCGCTTTCTATCCCACGGGCAGGAATCGTCCCCTTTGCGCTCGCCATCGC
TCCCCTCATCCATCGGGCGAATACCACACTACTTGCGCAGCTTATATATACAACATGCTTCTTCATTGTTTCCTACTGGC
TCTGCATGCGCCCACACACCTTAAGCAACACTCTCGGCAAAGTACTTACCCCCGCGCTCCTAGTACTCGTTCTCCTCCTC
TTCCTTGCCTCCTTCACTCCGACACTCGGTCCCTACCTCCCTGCACAGGGCGCTTACGCTACCCACATACCCTTCAGCCA
GGGATTCTTAGACGGTTACCTCACCACGGATGCACTCGCCTCCCTTATGTTCGGCAATATGATCCTTACCTATTTGCATC
GGACCCGCTACACAACCGCCCCTTTCCCTCCCACTCCAGCAAACACCCTCGCAGATATGCGCACCGTCGCCTGGATAGCA
GGGGTCATGCTCTTTTTTACCTATGGAGTACTGGCGCATCTCGGCGCACTCAGCGCCCGCCAACTCCCCCATACCGTTAA
CGGCGCGCACATACTCGCGTCGGTGTCACGCCACCTTTTCGGAAAAGCAGGCATCGCACTACTAGGACTGATCTTTACAA
TTGCCTGCCTAACTACCTGCGCCGGACTGCTTGTTTGCGTCAGCGAATACTTCCACAAACGCGCACCCCGTGTGTCTTAC
CTGTGCTGGATACGCCTGTTCACCATATCCAGCTTTGCGCTCGCAAATACAGGACTAGAACGTATACTGGCATACGGAAC
ACCCCTACTCATGATCCTATACCCAATCTCGCTGGTCCTCATTGGCATATCACACCTCGAGCGACTCATACGGATACCAC
GCGCCGCCTACCGCCTGACAGTATGGAGCGCAGGAACACTCAGCACCTGTGCAGTCGGTACGCCGCTTGTGGCGCACACC
CGGATAGGACACGTGTTGAATACACTCATACATACCCTTCCACTCGCACAGGAACAGCTCTGCTGGCTTATCCCCAGCGC
GGCAGTTCTTATACTTAGTACTGCGCATGCACGCTTACGTGAAAAAACATGCACGCCTCGCGGTACGCTACCCCTCACGG
ATAACTGA

Upstream 100 bases:

>100_bases
CCCACCACCCGCATAAAATTCTTTAGAACTCGCCTTTGTACCCGCACCACCCCTATTCACATACAAACGCTGCCCCGGCA
AAATACTCCCAGGAGGAATC

Downstream 100 bases:

>100_bases
CCACTGGATCTCACCATCTTGTGGAGATGGGGGGAATCGAACCCCCGTCCTAAAGAGCGAGTGCTGCGCGCCTACAGGTT
TAGCGGTGCGTACTGCGTTT

Product: amino acid ABC transporter, permease protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MIHTHTLSLSFMLFSFFFGAGNLILPPLLGKHAGTTLATALLGFATSAVLIPIAGLITIAHAGGIVPLSERVGKRFAHLY
PAITLLVIGPALSIPRAGIVPFALAIAPLIHRANTTLLAQLIYTTCFFIVSYWLCMRPHTLSNTLGKVLTPALLVLVLLL
FLASFTPTLGPYLPAQGAYATHIPFSQGFLDGYLTTDALASLMFGNMILTYLHRTRYTTAPFPPTPANTLADMRTVAWIA
GVMLFFTYGVLAHLGALSARQLPHTVNGAHILASVSRHLFGKAGIALLGLIFTIACLTTCAGLLVCVSEYFHKRAPRVSY
LCWIRLFTISSFALANTGLERILAYGTPLLMILYPISLVLIGISHLERLIRIPRAAYRLTVWSAGTLSTCAVGTPLVAHT
RIGHVLNTLIHTLPLAQEQLCWLIPSAAVLILSTAHARLREKTCTPRGTLPLTDN

Sequences:

>Translated_455_residues
MIHTHTLSLSFMLFSFFFGAGNLILPPLLGKHAGTTLATALLGFATSAVLIPIAGLITIAHAGGIVPLSERVGKRFAHLY
PAITLLVIGPALSIPRAGIVPFALAIAPLIHRANTTLLAQLIYTTCFFIVSYWLCMRPHTLSNTLGKVLTPALLVLVLLL
FLASFTPTLGPYLPAQGAYATHIPFSQGFLDGYLTTDALASLMFGNMILTYLHRTRYTTAPFPPTPANTLADMRTVAWIA
GVMLFFTYGVLAHLGALSARQLPHTVNGAHILASVSRHLFGKAGIALLGLIFTIACLTTCAGLLVCVSEYFHKRAPRVSY
LCWIRLFTISSFALANTGLERILAYGTPLLMILYPISLVLIGISHLERLIRIPRAAYRLTVWSAGTLSTCAVGTPLVAHT
RIGHVLNTLIHTLPLAQEQLCWLIPSAAVLILSTAHARLREKTCTPRGTLPLTDN
>Mature_455_residues
MIHTHTLSLSFMLFSFFFGAGNLILPPLLGKHAGTTLATALLGFATSAVLIPIAGLITIAHAGGIVPLSERVGKRFAHLY
PAITLLVIGPALSIPRAGIVPFALAIAPLIHRANTTLLAQLIYTTCFFIVSYWLCMRPHTLSNTLGKVLTPALLVLVLLL
FLASFTPTLGPYLPAQGAYATHIPFSQGFLDGYLTTDALASLMFGNMILTYLHRTRYTTAPFPPTPANTLADMRTVAWIA
GVMLFFTYGVLAHLGALSARQLPHTVNGAHILASVSRHLFGKAGIALLGLIFTIACLTTCAGLLVCVSEYFHKRAPRVSY
LCWIRLFTISSFALANTGLERILAYGTPLLMILYPISLVLIGISHLERLIRIPRAAYRLTVWSAGTLSTCAVGTPLVAHT
RIGHVLNTLIHTLPLAQEQLCWLIPSAAVLILSTAHARLREKTCTPRGTLPLTDN

Specific function: Component of the LIV-II transport system for branched- chain amino acids. BraB is specific for isoleucine, leucine and valine. The LIV-II transport system is coupled to sodium and lithium ions [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=449, Percent_Identity=30.0668151447661, Blast_Score=172, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 49096; Mature: 49096

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHTHTLSLSFMLFSFFFGAGNLILPPLLGKHAGTTLATALLGFATSAVLIPIAGLITIA
CCCCHHHHHHHHHHHHHHCCCCEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAGGIVPLSERVGKRFAHLYPAITLLVIGPALSIPRAGIVPFALAIAPLIHRANTTLLAQ
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LIYTTCFFIVSYWLCMRPHTLSNTLGKVLTPALLVLVLLLFLASFTPTLGPYLPAQGAYA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
THIPFSQGFLDGYLTTDALASLMFGNMILTYLHRTRYTTAPFPPTPANTLADMRTVAWIA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
GVMLFFTYGVLAHLGALSARQLPHTVNGAHILASVSRHLFGKAGIALLGLIFTIACLTTC
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLLVCVSEYFHKRAPRVSYLCWIRLFTISSFALANTGLERILAYGTPLLMILYPISLVL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
IGISHLERLIRIPRAAYRLTVWSAGTLSTCAVGTPLVAHTRIGHVLNTLIHTLPLAQEQL
HHHHHHHHHHHCCHHHHEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
CWLIPSAAVLILSTAHARLREKTCTPRGTLPLTDN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MIHTHTLSLSFMLFSFFFGAGNLILPPLLGKHAGTTLATALLGFATSAVLIPIAGLITIA
CCCCHHHHHHHHHHHHHHCCCCEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAGGIVPLSERVGKRFAHLYPAITLLVIGPALSIPRAGIVPFALAIAPLIHRANTTLLAQ
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LIYTTCFFIVSYWLCMRPHTLSNTLGKVLTPALLVLVLLLFLASFTPTLGPYLPAQGAYA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
THIPFSQGFLDGYLTTDALASLMFGNMILTYLHRTRYTTAPFPPTPANTLADMRTVAWIA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
GVMLFFTYGVLAHLGALSARQLPHTVNGAHILASVSRHLFGKAGIALLGLIFTIACLTTC
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLLVCVSEYFHKRAPRVSYLCWIRLFTISSFALANTGLERILAYGTPLLMILYPISLVL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
IGISHLERLIRIPRAAYRLTVWSAGTLSTCAVGTPLVAHTRIGHVLNTLIHTLPLAQEQL
HHHHHHHHHHHCCHHHHEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
CWLIPSAAVLILSTAHARLREKTCTPRGTLPLTDN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2111004; 10984043 [H]