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The map label for this gene is crp [H]

Identifier: 189025496

GI number: 189025496

Start: 274522

End: 275196

Strand: Direct

Name: crp [H]

Synonym: TPASS_0262

Alternate gene names: 189025496

Gene position: 274522-275196 (Clockwise)

Preceding gene: 189025495

Following gene: 189025497

Centisome position: 24.09

GC content: 51.85

Gene sequence:

>675_bases
GTGAGGAGGCGTCGCATGGCTGATGCCTTTGCCTCGTTTACGCGCTTTGCCAAAAAGTTTCAGCGGGGTTCTGTTATCTT
CGCAGAGTTTGATGTGGGGGAGAGTTTCTACCTCGTGCAGAGCGGGCGGGTGCGTCTAATTAAAATTATCAACGGAAAGG
AGCGCTACTTGGATGTGTTGCAACCGGGGGAGATTTTTGGAGAGATGTCTATTCTCGACAACTCACCGCGTTCGGCGTCT
GCGGTTGCGTACGACGATGTTGTGGCGCTGGAGTTCAATCGGGAGAATTTCGAAGTGCTCATGATGGGTAATCCTGCTAT
TGCAATGCGGCTTTTGAAAACGTTTGTGCGGCGTATTTACACCCAGAAGCGGCGTTTTATGATTTTAACGATTCAAGATA
GTACTGCGCGTGTGGGGGATGTTTTCTTGATGTTGGATGAAACGCAACCGCACGTGGATCGCAGCAGCGACGCGCGTTCT
TTTGATATCAGCATCGAAGAGATAGCCCGGTGGGCTGGTCTTTCTGACAGGGAGGTACAGGACGCGCTGCGCAGGCTTTC
TGATCAGCGGAAGGTTGAGTGGCAAGAGCGCCGGATAGTAGTTAAAAATATGACGGATTTGAACCGCTACGTGACCATGC
GCCGGGGTAAGGGTGATGGTTTGCCCTTAGGCTGA

Upstream 100 bases:

>100_bases
GGTATGGGGAGGCCGGATAAGGCGATTTCTTTTTACGACAAGGCGCTTTCTACCGCTCCTGAGTCTTTAGTTCCGCGCAT
AAAAGAGCTCCAGCGTTCCT

Downstream 100 bases:

>100_bases
ACCGTTGTAGCGGAGTGCCCATTCGTTTTCGCTGTCTGCTGAGGAGACGCTCTTTTTCCCTGGGGTGTACCGTGCGCGTA
CATACAAAGGCGCTGCGCGG

Product: catabolite gene activator

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MRRRRMADAFASFTRFAKKFQRGSVIFAEFDVGESFYLVQSGRVRLIKIINGKERYLDVLQPGEIFGEMSILDNSPRSAS
AVAYDDVVALEFNRENFEVLMMGNPAIAMRLLKTFVRRIYTQKRRFMILTIQDSTARVGDVFLMLDETQPHVDRSSDARS
FDISIEEIARWAGLSDREVQDALRRLSDQRKVEWQERRIVVKNMTDLNRYVTMRRGKGDGLPLG

Sequences:

>Translated_224_residues
MRRRRMADAFASFTRFAKKFQRGSVIFAEFDVGESFYLVQSGRVRLIKIINGKERYLDVLQPGEIFGEMSILDNSPRSAS
AVAYDDVVALEFNRENFEVLMMGNPAIAMRLLKTFVRRIYTQKRRFMILTIQDSTARVGDVFLMLDETQPHVDRSSDARS
FDISIEEIARWAGLSDREVQDALRRLSDQRKVEWQERRIVVKNMTDLNRYVTMRRGKGDGLPLG
>Mature_224_residues
MRRRRMADAFASFTRFAKKFQRGSVIFAEFDVGESFYLVQSGRVRLIKIINGKERYLDVLQPGEIFGEMSILDNSPRSAS
AVAYDDVVALEFNRENFEVLMMGNPAIAMRLLKTFVRRIYTQKRRFMILTIQDSTARVGDVFLMLDETQPHVDRSSDARS
FDISIEEIARWAGLSDREVQDALRRLSDQRKVEWQERRIVVKNMTDLNRYVTMRRGKGDGLPLG

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=185, Percent_Identity=29.1891891891892, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26000; Mature: 26000

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: PS00889 CNMP_BINDING_2 ; PS50042 CNMP_BINDING_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRRRMADAFASFTRFAKKFQRGSVIFAEFDVGESFYLVQSGRVRLIKIINGKERYLDVL
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCEEEEEEECCHHHHHHHC
QPGEIFGEMSILDNSPRSASAVAYDDVVALEFNRENFEVLMMGNPAIAMRLLKTFVRRIY
CCHHHHEEHHHCCCCCCCCCEEEECCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHH
TQKRRFMILTIQDSTARVGDVFLMLDETQPHVDRSSDARSFDISIEEIARWAGLSDREVQ
HCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCEECCCHHHHHHHCCCCCHHHH
DALRRLSDQRKVEWQERRIVVKNMTDLNRYVTMRRGKGDGLPLG
HHHHHHHHHHCCCHHHCEEEECCHHHHHHHEEEECCCCCCCCCC
>Mature Secondary Structure
MRRRRMADAFASFTRFAKKFQRGSVIFAEFDVGESFYLVQSGRVRLIKIINGKERYLDVL
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCEEEEEEECCHHHHHHHC
QPGEIFGEMSILDNSPRSASAVAYDDVVALEFNRENFEVLMMGNPAIAMRLLKTFVRRIY
CCHHHHEEHHHCCCCCCCCCEEEECCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHH
TQKRRFMILTIQDSTARVGDVFLMLDETQPHVDRSSDARSFDISIEEIARWAGLSDREVQ
HCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCEECCCHHHHHHHCCCCCHHHH
DALRRLSDQRKVEWQERRIVVKNMTDLNRYVTMRRGKGDGLPLG
HHHHHHHHHHCCCHHHCEEEECCHHHHHHHEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]