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The map label for this gene is amyA [H]

Identifier: 189025380

GI number: 189025380

Start: 170462

End: 172321

Strand: Reverse

Name: amyA [H]

Synonym: TPASS_0147

Alternate gene names: 189025380

Gene position: 172321-170462 (Counterclockwise)

Preceding gene: 189025381

Following gene: 189025378

Centisome position: 15.12

GC content: 44.89

Gene sequence:

>1860_bases
ATGAAAAAAGGAGACAATACCAAAATACGTATCGCATTTGCTGCGAAGTTAACGGAAGCATGTGTTCAGTCCCTGCACGC
AGAGCACGATCCTTCTCAGTACCAGGCATTTTTTTCAGGTCTGTGCAGGCACGAACAACTCCCACTGACGGTGCACATGA
CCGGTTCTTTTTTTCAATTTATACAAAAAAAGAATTCTCCCTATCCTTATCTTATCAACAATCTGCTTGGTTCCAAACGG
CTTGAATTACTCAGCGGCGGTTTTTACCAGCCGTATCTTCCCCTCCTCCCTCAGGCGGATGTAATTAGTCAAATTGAGCT
ACTCACTTCTACCTTGCGAGAACACGTTATAAAACGCCCACGGGGCTGCTTTCTGGAAGCATCTGCATGGAGCGCCGCGC
TCATTCCCTCACTCGTAAAGGCCGGAATGGAATACTGTCTCCTAGATTACCGTCTCTTCGAAGCCTCTGATATGCCCGCT
ACTAAACCTGCAGTACTTGAGGACAGCAGCAAATGCCTTGTTGCATTACCGTACCGCGAACTGACGATTGACACCCCCGC
TGAAACATTCTATGCGCAACTTGTCGCACGGTACGGCGACACCCCCGGTACTGTGCTCATTTTCATGCTTCCGCTCGACT
GCTACCAAAAATTATTTTTTACACACGACAATGCATCCACTTGGTTTAAAACATTTATTCAATACGTGCAAAAACCGAAC
TCTCCCTTTCGTTTAACGCATACTGGAGAAATATACAAAGAGCCTCCTGTTTGTCTCGGATACATCGCTTCGAATATGGT
GCGAAACGATGTCCTTTTGCACAAAACGGCCAAGCAATTAATAATATGCAGCACACACGCGCGGTTGTTGTACGCAAAGA
TGATGTACGTACACAATATTGCTATGCAAATTAAAGGAGATAAACAACGCAAGCATCATGCACTGTCCGAATTATGGAAA
GCAGAACAGGGAGTTTTTTTTATTTCTTCTGATCGGGGGTACGCATCCGCAGGAAGCGCGCAACGTACTGCATATAAATA
CCTCCTGACTGCAGAAAAAGTGGCACGCACTCCTGGTGTGTTTAATAATTCTCTCATTACGTTCGACTTTAACTTCGATG
GTGTACGGGAGTTTCTCTCTCAAAGGGAGCTGATGAATATGTACGTCCAATTACCAGGTGGACTGATTTTTGAATTTGAC
ATTTTTTCATCGTATAAAAACTATGCAGCAGTCCCTCGACAACCGGGAGGCATTTTTATTGACTATGTGCTCTCTGAGCA
AGATGTTGTGCACCTGCGAGAAAACCCCGGCCATGCCGTGCCCACGGTGTTCTCGCATACCCCTTATCAGGAAATAGGAT
TCGATCCTATAAAGCACGAGCTTACACTTAGGGCGGAAGGTATCTTTGAAGAATTCAATCAGCCTGTTTCATTAAAAAAA
CAGTACTTTTTTAAGTCTGAAGGCGTACAAGTTCAGTACATCTTAAAAAACGAAAGTCCCCTCAATCTTTCCGCATTTTT
TGCAGTGGAAATGAATCTTGCCGTACACGGTACTGATGACGCTGCACCCGAGCTTGTGGTTTTTTCGGAAAATGAAAAGT
ACACTGCAGCAATTACCCGTGATCATTTTCCCTGCATTTCATGGATGCGTCTTACCGATCCTGAGGGGAAGACTGTCTTT
ACGATCGAAGGAAACGAGAACCCTGACGTGGCTATCACGCCTCTGTGCGAAAGTACATCGAACCAGTATGCAAACACCGA
AGGCATCAAGTGTCTTTTCTACTGGAAAGTCGACTTGAACGCTGGATACGAAACAGAAAAGATGCTCTTTCTCAAAGCCT
TTTCTACAAAGAAAAGGTAA

Upstream 100 bases:

>100_bases
CACTTCTCTTAGCAAGTATGGGTTTTATTGCACTGAGCCTGTACGGTACTGACGAGCTGTGGCTTTTTCCGATACCCGCG
GCGGGGATGAGAACCCGATT

Downstream 100 bases:

>100_bases
CGTACTGATTCAATTACCAAAGCATAACGCACCCAGCGCGCCCAGGAATATCAAGAAAACTGGGTGTATCCTTTTGAAGA
AAAAGCTCAGTGCGCATAAC

Product: alpha-amylase 1

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MKKGDNTKIRIAFAAKLTEACVQSLHAEHDPSQYQAFFSGLCRHEQLPLTVHMTGSFFQFIQKKNSPYPYLINNLLGSKR
LELLSGGFYQPYLPLLPQADVISQIELLTSTLREHVIKRPRGCFLEASAWSAALIPSLVKAGMEYCLLDYRLFEASDMPA
TKPAVLEDSSKCLVALPYRELTIDTPAETFYAQLVARYGDTPGTVLIFMLPLDCYQKLFFTHDNASTWFKTFIQYVQKPN
SPFRLTHTGEIYKEPPVCLGYIASNMVRNDVLLHKTAKQLIICSTHARLLYAKMMYVHNIAMQIKGDKQRKHHALSELWK
AEQGVFFISSDRGYASAGSAQRTAYKYLLTAEKVARTPGVFNNSLITFDFNFDGVREFLSQRELMNMYVQLPGGLIFEFD
IFSSYKNYAAVPRQPGGIFIDYVLSEQDVVHLRENPGHAVPTVFSHTPYQEIGFDPIKHELTLRAEGIFEEFNQPVSLKK
QYFFKSEGVQVQYILKNESPLNLSAFFAVEMNLAVHGTDDAAPELVVFSENEKYTAAITRDHFPCISWMRLTDPEGKTVF
TIEGNENPDVAITPLCESTSNQYANTEGIKCLFYWKVDLNAGYETEKMLFLKAFSTKKR

Sequences:

>Translated_619_residues
MKKGDNTKIRIAFAAKLTEACVQSLHAEHDPSQYQAFFSGLCRHEQLPLTVHMTGSFFQFIQKKNSPYPYLINNLLGSKR
LELLSGGFYQPYLPLLPQADVISQIELLTSTLREHVIKRPRGCFLEASAWSAALIPSLVKAGMEYCLLDYRLFEASDMPA
TKPAVLEDSSKCLVALPYRELTIDTPAETFYAQLVARYGDTPGTVLIFMLPLDCYQKLFFTHDNASTWFKTFIQYVQKPN
SPFRLTHTGEIYKEPPVCLGYIASNMVRNDVLLHKTAKQLIICSTHARLLYAKMMYVHNIAMQIKGDKQRKHHALSELWK
AEQGVFFISSDRGYASAGSAQRTAYKYLLTAEKVARTPGVFNNSLITFDFNFDGVREFLSQRELMNMYVQLPGGLIFEFD
IFSSYKNYAAVPRQPGGIFIDYVLSEQDVVHLRENPGHAVPTVFSHTPYQEIGFDPIKHELTLRAEGIFEEFNQPVSLKK
QYFFKSEGVQVQYILKNESPLNLSAFFAVEMNLAVHGTDDAAPELVVFSENEKYTAAITRDHFPCISWMRLTDPEGKTVF
TIEGNENPDVAITPLCESTSNQYANTEGIKCLFYWKVDLNAGYETEKMLFLKAFSTKKR
>Mature_619_residues
MKKGDNTKIRIAFAAKLTEACVQSLHAEHDPSQYQAFFSGLCRHEQLPLTVHMTGSFFQFIQKKNSPYPYLINNLLGSKR
LELLSGGFYQPYLPLLPQADVISQIELLTSTLREHVIKRPRGCFLEASAWSAALIPSLVKAGMEYCLLDYRLFEASDMPA
TKPAVLEDSSKCLVALPYRELTIDTPAETFYAQLVARYGDTPGTVLIFMLPLDCYQKLFFTHDNASTWFKTFIQYVQKPN
SPFRLTHTGEIYKEPPVCLGYIASNMVRNDVLLHKTAKQLIICSTHARLLYAKMMYVHNIAMQIKGDKQRKHHALSELWK
AEQGVFFISSDRGYASAGSAQRTAYKYLLTAEKVARTPGVFNNSLITFDFNFDGVREFLSQRELMNMYVQLPGGLIFEFD
IFSSYKNYAAVPRQPGGIFIDYVLSEQDVVHLRENPGHAVPTVFSHTPYQEIGFDPIKHELTLRAEGIFEEFNQPVSLKK
QYFFKSEGVQVQYILKNESPLNLSAFFAVEMNLAVHGTDDAAPELVVFSENEKYTAAITRDHFPCISWMRLTDPEGKTVF
TIEGNENPDVAITPLCESTSNQYANTEGIKCLFYWKVDLNAGYETEKMLFLKAFSTKKR

Specific function: This amylase is a highly liquefying-type:oligomers appeared at the beginning of incubation, followed by a graded decrease in the amounts of maltotriose, maltose and glucose in prolonged incubation [H]

COG id: COG1449

COG function: function code G; Alpha-amylase/alpha-mannosidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 57 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015179
- InterPro:   IPR015178
- InterPro:   IPR011013
- InterPro:   IPR011330
- InterPro:   IPR000602
- InterPro:   IPR004300
- ProDom:   PD014405 [H]

Pfam domain/function: PF09094 DUF1925; PF09095 DUF1926; PF03065 Glyco_hydro_57 [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 70372; Mature: 70372

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGDNTKIRIAFAAKLTEACVQSLHAEHDPSQYQAFFSGLCRHEQLPLTVHMTGSFFQF
CCCCCCCEEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHH
IQKKNSPYPYLINNLLGSKRLELLSGGFYQPYLPLLPQADVISQIELLTSTLREHVIKRP
HHHCCCCCCHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
RGCFLEASAWSAALIPSLVKAGMEYCLLDYRLFEASDMPATKPAVLEDSSKCLVALPYRE
CCCEEECCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCEECCCCCEEEEECCEE
LTIDTPAETFYAQLVARYGDTPGTVLIFMLPLDCYQKLFFTHDNASTWFKTFIQYVQKPN
EEECCCHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHEECCCCHHHHHHHHHHHHCCC
SPFRLTHTGEIYKEPPVCLGYIASNMVRNDVLLHKTAKQLIICSTHARLLYAKMMYVHNI
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHE
AMQIKGDKQRKHHALSELWKAEQGVFFISSDRGYASAGSAQRTAYKYLLTAEKVARTPGV
EEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
FNNSLITFDFNFDGVREFLSQRELMNMYVQLPGGLIFEFDIFSSYKNYAAVPRQPGGIFI
CCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCCCCCCCEEE
DYVLSEQDVVHLRENPGHAVPTVFSHTPYQEIGFDPIKHELTLRAEGIFEEFNQPVSLKK
EEEECCCCEEEEECCCCCCCCEEECCCCHHHCCCCCCCCEEEEEECHHHHHCCCCCCHHH
QYFFKSEGVQVQYILKNESPLNLSAFFAVEMNLAVHGTDDAAPELVVFSENEKYTAAITR
HHEECCCCCEEEEEEECCCCCCEEEEEEEEEEEEEECCCCCCCEEEEEECCCEEEEEEEC
DHFPCISWMRLTDPEGKTVFTIEGNENPDVAITPLCESTSNQYANTEGIKCLFYWKVDLN
CCCCCEEEEEEECCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEECC
AGYETEKMLFLKAFSTKKR
CCCCCHHEEEEEECCCCCC
>Mature Secondary Structure
MKKGDNTKIRIAFAAKLTEACVQSLHAEHDPSQYQAFFSGLCRHEQLPLTVHMTGSFFQF
CCCCCCCEEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHH
IQKKNSPYPYLINNLLGSKRLELLSGGFYQPYLPLLPQADVISQIELLTSTLREHVIKRP
HHHCCCCCCHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
RGCFLEASAWSAALIPSLVKAGMEYCLLDYRLFEASDMPATKPAVLEDSSKCLVALPYRE
CCCEEECCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCEECCCCCEEEEECCEE
LTIDTPAETFYAQLVARYGDTPGTVLIFMLPLDCYQKLFFTHDNASTWFKTFIQYVQKPN
EEECCCHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHEECCCCHHHHHHHHHHHHCCC
SPFRLTHTGEIYKEPPVCLGYIASNMVRNDVLLHKTAKQLIICSTHARLLYAKMMYVHNI
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHE
AMQIKGDKQRKHHALSELWKAEQGVFFISSDRGYASAGSAQRTAYKYLLTAEKVARTPGV
EEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
FNNSLITFDFNFDGVREFLSQRELMNMYVQLPGGLIFEFDIFSSYKNYAAVPRQPGGIFI
CCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCCCCCCCEEE
DYVLSEQDVVHLRENPGHAVPTVFSHTPYQEIGFDPIKHELTLRAEGIFEEFNQPVSLKK
EEEECCCCEEEEECCCCCCCCEEECCCCHHHCCCCCCCCEEEEEECHHHHHCCCCCCHHH
QYFFKSEGVQVQYILKNESPLNLSAFFAVEMNLAVHGTDDAAPELVVFSENEKYTAAITR
HHEECCCCCEEEEEEECCCCCCEEEEEEEEEEEEEECCCCCCCEEEEEECCCEEEEEEEC
DHFPCISWMRLTDPEGKTVFTIEGNENPDVAITPLCESTSNQYANTEGIKCLFYWKVDLN
CCCCCEEEEEEECCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEECC
AGYETEKMLFLKAFSTKKR
CCCCCHHEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2453362 [H]