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The map label for this gene is thiB [H]

Identifier: 189025378

GI number: 189025378

Start: 165970

End: 166977

Strand: Reverse

Name: thiB [H]

Synonym: TPASS_0144

Alternate gene names: 189025378

Gene position: 166977-165970 (Counterclockwise)

Preceding gene: 189025380

Following gene: 189025377

Centisome position: 14.65

GC content: 57.74

Gene sequence:

>1008_bases
ATGGCTCACCGTATGCGTGTTGTCTTCTGCTGCGCTGCAGCGTTGTCGCTCTGCTGCTGTAGTCGCAGCCGGACGGACGC
GCCGTCGCTTGTTATCTATGTCTACGACTCCTTTGCTTCTGAATGGGGACCTGGCCCTGAACTTGCGCGGCGCTTCTACG
CGAAAACAGGGTATTCCGTTCAGTACGTGGTGTTTGAGGACGTCACGCGCGCTATTCATCGTGCCATCCGCGATCAGCAC
ACGCCGTCATCCAGAGGTGCCGATCTTATCATCGGGGTTGACCAATACACCCTCCCCCTTGCGCGTGAAGCAGATCTCTT
CAGTCCCTACCAACCGGCCCTTCCGCTTCCTCACGCCTTGCGCATAGACCCACACAACCTCGCAGTCCCCTATGACTGGG
GGTACGTCGCCTTCATGTTCAACACAGACGCTGCGCTTCCCGAGCCACAGCGGCTTTCAGATCTTGCAAATCCCGTCTAC
GCAAAAAGCATTGCACTTGCAGACCCGCATACTAACGTGCTTGGTACGATCTTTATCGGTTGGGCCCACACATCACTCGG
CGCTGCATGGCCTGCGTTCTGGAATACGCTCTATCGCGCGAATCTCCTTGCGCTCGCCCCGAGCTGGAGCGCCGCATACG
GCTTGTTCACCGCAGGGGAAGCCGCACTGGTACTTAGTTACACCACAAGTGCAGCGTACCACCTGCACCATGACAACCAT
ACGCGCTTTAAACCTCTCCTCTTTCCCGAGGGCCACCCCATGCATATCGAAGGTATTAGCCTGGTGAAGCGTACGAAACA
TGCGCGGGTTGCTACTCAGTTTATCGACTTTCTGCTCAGCACTGAAGCGCAGGAGCTCCTTCCCCTCACTCAGTGGATGT
ACCCGGCCAATCCTGACGTACCGTTGCCTGCCGCATTCTCTGCAGTCCCTCAACCTGCTGCCTGCGTGTCTCTCTCGCCG
CAGATGTGCCGCGCTGCAGCAGCGGCATTACACGCATCAACCCCATGA

Upstream 100 bases:

>100_bases
TGTACCGAATGCACTCCCTTTGAGCAAGACAGCAGCACTGTACCCCTCACCCTGGATAGGAGCTCTAGAGCTATGCTATA
GTCCCCGGCGGGGAGAGGCT

Downstream 100 bases:

>100_bases
CCACGCTCTTACATCCGTGCCGCCGCTCGTGTGCGTCTGGAGCTCTCTATCTGACAAGAGGAAGGGACGCGCGCAACTAC
TGTGTTGCTCTCATCTGTGC

Product: thiamine ABC transporter, thiamine-binding periplasmic protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MAHRMRVVFCCAAALSLCCCSRSRTDAPSLVIYVYDSFASEWGPGPELARRFYAKTGYSVQYVVFEDVTRAIHRAIRDQH
TPSSRGADLIIGVDQYTLPLAREADLFSPYQPALPLPHALRIDPHNLAVPYDWGYVAFMFNTDAALPEPQRLSDLANPVY
AKSIALADPHTNVLGTIFIGWAHTSLGAAWPAFWNTLYRANLLALAPSWSAAYGLFTAGEAALVLSYTTSAAYHLHHDNH
TRFKPLLFPEGHPMHIEGISLVKRTKHARVATQFIDFLLSTEAQELLPLTQWMYPANPDVPLPAAFSAVPQPAACVSLSP
QMCRAAAAALHASTP

Sequences:

>Translated_335_residues
MAHRMRVVFCCAAALSLCCCSRSRTDAPSLVIYVYDSFASEWGPGPELARRFYAKTGYSVQYVVFEDVTRAIHRAIRDQH
TPSSRGADLIIGVDQYTLPLAREADLFSPYQPALPLPHALRIDPHNLAVPYDWGYVAFMFNTDAALPEPQRLSDLANPVY
AKSIALADPHTNVLGTIFIGWAHTSLGAAWPAFWNTLYRANLLALAPSWSAAYGLFTAGEAALVLSYTTSAAYHLHHDNH
TRFKPLLFPEGHPMHIEGISLVKRTKHARVATQFIDFLLSTEAQELLPLTQWMYPANPDVPLPAAFSAVPQPAACVSLSP
QMCRAAAAALHASTP
>Mature_334_residues
AHRMRVVFCCAAALSLCCCSRSRTDAPSLVIYVYDSFASEWGPGPELARRFYAKTGYSVQYVVFEDVTRAIHRAIRDQHT
PSSRGADLIIGVDQYTLPLAREADLFSPYQPALPLPHALRIDPHNLAVPYDWGYVAFMFNTDAALPEPQRLSDLANPVYA
KSIALADPHTNVLGTIFIGWAHTSLGAAWPAFWNTLYRANLLALAPSWSAAYGLFTAGEAALVLSYTTSAAYHLHHDNHT
RFKPLLFPEGHPMHIEGISLVKRTKHARVATQFIDFLLSTEAQELLPLTQWMYPANPDVPLPAAFSAVPQPAACVSLSPQ
MCRAAAAALHASTP

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=296, Percent_Identity=32.4324324324324, Blast_Score=164, Evalue=7e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36813; Mature: 36682

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHRMRVVFCCAAALSLCCCSRSRTDAPSLVIYVYDSFASEWGPGPELARRFYAKTGYSV
CCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEHHHHCCCCCHHHHHHHHHCCCCEE
QYVVFEDVTRAIHRAIRDQHTPSSRGADLIIGVDQYTLPLAREADLFSPYQPALPLPHAL
EEEHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEE
RIDPHNLAVPYDWGYVAFMFNTDAALPEPQRLSDLANPVYAKSIALADPHTNVLGTIFIG
EECCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHCCCHHHHEEEECCCCCCEEEEEEEE
WAHTSLGAAWPAFWNTLYRANLLALAPSWSAAYGLFTAGEAALVLSYTTSAAYHLHHDNH
EHHHHHCCHHHHHHHHHHHCCCEEECCCCHHHHHEEECCCEEEEEEEECCEEEEEECCCC
TRFKPLLFPEGHPMHIEGISLVKRTKHARVATQFIDFLLSTEAQELLPLTQWMYPANPDV
CCCCEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCC
PLPAAFSAVPQPAACVSLSPQMCRAAAAALHASTP
CCCHHHHCCCCCCCEEECCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AHRMRVVFCCAAALSLCCCSRSRTDAPSLVIYVYDSFASEWGPGPELARRFYAKTGYSV
CCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEHHHHCCCCCHHHHHHHHHCCCCEE
QYVVFEDVTRAIHRAIRDQHTPSSRGADLIIGVDQYTLPLAREADLFSPYQPALPLPHAL
EEEHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEE
RIDPHNLAVPYDWGYVAFMFNTDAALPEPQRLSDLANPVYAKSIALADPHTNVLGTIFIG
EECCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHCCCHHHHEEEECCCCCCEEEEEEEE
WAHTSLGAAWPAFWNTLYRANLLALAPSWSAAYGLFTAGEAALVLSYTTSAAYHLHHDNH
EHHHHHCCHHHHHHHHHHHCCCEEECCCCHHHHHEEECCCEEEEEEEECCEEEEEECCCC
TRFKPLLFPEGHPMHIEGISLVKRTKHARVATQFIDFLLSTEAQELLPLTQWMYPANPDV
CCCCEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCC
PLPAAFSAVPQPAACVSLSPQMCRAAAAALHASTP
CCCHHHHCCCCCCCEEECCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA