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The map label for this gene is pfk [H]

Identifier: 189025342

GI number: 189025342

Start: 122272

End: 123657

Strand: Reverse

Name: pfk [H]

Synonym: TPASS_0108

Alternate gene names: 189025342

Gene position: 123657-122272 (Counterclockwise)

Preceding gene: 189025343

Following gene: 189025341

Centisome position: 10.85

GC content: 51.08

Gene sequence:

>1386_bases
ATGGCACATCTTCCTAAAGAGTACGATTTTTCTATAGAGTCATTGGGGGAAAGCAAAATTCCCTCTCCCATCTACCTGTC
TCACACCCTTGGCGACTTCATTCCTAACTACGTCAGTGACAATGAGTACATCAGCCATGAACTGAGTGCGCGTCTGGGGG
AGACGGTAGGGCCCTTTACTCATAAAAACTTGATGGAGCGTGCGGGCCCGCGCCAGAAGATTTTCTTCAACCCGCATCAC
GTTCATGCAGGTATTGTCACCTGTGGAGGGCTCTGTCCCGGCCTCAACGATGTCATTCGCGCCATCGTCCGCTGCCTTTG
GGGCCGCTATGGCGTTAAGCGCATTAGTGGTATCCGCTTTGGCTATAAGGGCCTCTTGCCCGATTACAACTTCGATATCC
TGCCGCTCACCCCTGAGGTCATCGATAACTGCCACAAAACAGGTGGTTCGCTGCTAGGCACCTCGCGCGGTGGGGGGAAC
CGTGTGGTTGACATCGTCGATGGTATCGAGCGGCTCAACCTGCACATTCTCTTTATCATTGGGGGGGATGGGAGTCAGAA
GGGAGCAAAAGAGATTGCCGACGAAATAAAACACCGCAACCTTAAGATTTCCATCATCGGCATTCCCAAAACGGTGGACA
ACGACATCTCTTTTGTTCAAAAGTCATTTGGCTTCGATACTGCTATTGTCAAAGCAACAGAGGCAGTTGCCGCAGCGCAC
ATGGAGGCTCGTTCACAGATTAACGGCATTGGACTGGTCAAACTCATGGGGCGTGAGTCTGGATTCATCGCTACGTACAC
GGCGATTGCGAGCCATGAGACAAACTTCGTACTCATCCCTGAAGTGAGCTTTGACCTTGACGGTCCAAATGGGCTTTTGG
CTCACCTAGAGAAGCGAATCGCGCTACGTAAGCATGCGGTGCTCGTTGTAGCAGAAGGTGCGGGGCAAGACCTAATGGTA
AATGCGGACGGTGTGCCCTCCGGTGATTCCCAAGGAGGTAGTTTGCGCGTGAGCAGCGGCACGGATGCGTCGGGTAACAA
GCGCCTTGCGGATATCGGTTTGTTCTTAAAAGAAAAGATTGGAGTCTATTTCAAAGAAAAGCGTATACACATCAATTTAA
AGTACATCGATCCGAGCTACTTGATTCGTTCCGCGGTTGCTGCGCCCATAGATTCGATTTACTGCGAACGCCTCGGTAAC
AATGCGGTGCATGCGGCGATGTGCGGTAAAACGAAAATGATCATTGGCTTGGTGCACAATAAGTTTGTACACCTCCCAAT
CGATGTGGTGGTGTGCCAACGCAAACACGTAAATCCTGAAGGATCGTTGTGGCGCGACGCCCTTGATGCAACAGGACAGC
CGATTGTGATGAAAAATATTATCTAG

Upstream 100 bases:

>100_bases
AACACCCAGGGCATCTTCGCATCTTTAATTCATATTGCATCTTTCACCCCATTGCGTTAGCCTGAGGGAGTCTCTACGCA
CGCGGTACGCGGAGGACCCC

Downstream 100 bases:

>100_bases
GAGAGCGAAGGATGCGCTCCTCACAGCTGTGTGCAGGAAGCGAGCAGGGGGGGGGGTGCTTTTTCCCCTGTTTTTTGCGT
GCTTGACGGCGGGCGAACAC

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MAHLPKEYDFSIESLGESKIPSPIYLSHTLGDFIPNYVSDNEYISHELSARLGETVGPFTHKNLMERAGPRQKIFFNPHH
VHAGIVTCGGLCPGLNDVIRAIVRCLWGRYGVKRISGIRFGYKGLLPDYNFDILPLTPEVIDNCHKTGGSLLGTSRGGGN
RVVDIVDGIERLNLHILFIIGGDGSQKGAKEIADEIKHRNLKISIIGIPKTVDNDISFVQKSFGFDTAIVKATEAVAAAH
MEARSQINGIGLVKLMGRESGFIATYTAIASHETNFVLIPEVSFDLDGPNGLLAHLEKRIALRKHAVLVVAEGAGQDLMV
NADGVPSGDSQGGSLRVSSGTDASGNKRLADIGLFLKEKIGVYFKEKRIHINLKYIDPSYLIRSAVAAPIDSIYCERLGN
NAVHAAMCGKTKMIIGLVHNKFVHLPIDVVVCQRKHVNPEGSLWRDALDATGQPIVMKNII

Sequences:

>Translated_461_residues
MAHLPKEYDFSIESLGESKIPSPIYLSHTLGDFIPNYVSDNEYISHELSARLGETVGPFTHKNLMERAGPRQKIFFNPHH
VHAGIVTCGGLCPGLNDVIRAIVRCLWGRYGVKRISGIRFGYKGLLPDYNFDILPLTPEVIDNCHKTGGSLLGTSRGGGN
RVVDIVDGIERLNLHILFIIGGDGSQKGAKEIADEIKHRNLKISIIGIPKTVDNDISFVQKSFGFDTAIVKATEAVAAAH
MEARSQINGIGLVKLMGRESGFIATYTAIASHETNFVLIPEVSFDLDGPNGLLAHLEKRIALRKHAVLVVAEGAGQDLMV
NADGVPSGDSQGGSLRVSSGTDASGNKRLADIGLFLKEKIGVYFKEKRIHINLKYIDPSYLIRSAVAAPIDSIYCERLGN
NAVHAAMCGKTKMIIGLVHNKFVHLPIDVVVCQRKHVNPEGSLWRDALDATGQPIVMKNII
>Mature_460_residues
AHLPKEYDFSIESLGESKIPSPIYLSHTLGDFIPNYVSDNEYISHELSARLGETVGPFTHKNLMERAGPRQKIFFNPHHV
HAGIVTCGGLCPGLNDVIRAIVRCLWGRYGVKRISGIRFGYKGLLPDYNFDILPLTPEVIDNCHKTGGSLLGTSRGGGNR
VVDIVDGIERLNLHILFIIGGDGSQKGAKEIADEIKHRNLKISIIGIPKTVDNDISFVQKSFGFDTAIVKATEAVAAAHM
EARSQINGIGLVKLMGRESGFIATYTAIASHETNFVLIPEVSFDLDGPNGLLAHLEKRIALRKHAVLVVAEGAGQDLMVN
ADGVPSGDSQGGSLRVSSGTDASGNKRLADIGLFLKEKIGVYFKEKRIHINLKYIDPSYLIRSAVAAPIDSIYCERLGNN
AVHAAMCGKTKMIIGLVHNKFVHLPIDVVVCQRKHVNPEGSLWRDALDATGQPIVMKNII

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453619, Length=368, Percent_Identity=24.7282608695652, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI266453768, Length=368, Percent_Identity=24.7282608695652, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI266453748, Length=368, Percent_Identity=24.7282608695652, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI4505749, Length=368, Percent_Identity=24.7282608695652, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI11321601, Length=253, Percent_Identity=25.6916996047431, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790350, Length=346, Percent_Identity=26.0115606936416, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17558788, Length=375, Percent_Identity=27.4666666666667, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25147584, Length=258, Percent_Identity=27.1317829457364, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321679, Length=286, Percent_Identity=25.8741258741259, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17647809, Length=253, Percent_Identity=27.6679841897233, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24652337, Length=252, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI28573326, Length=252, Percent_Identity=27.3809523809524, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 50163; Mature: 50031

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHLPKEYDFSIESLGESKIPSPIYLSHTLGDFIPNYVSDNEYISHELSARLGETVGPFT
CCCCCCCCCCCHHHCCCCCCCCCEEEHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHH
HKNLMERAGPRQKIFFNPHHVHAGIVTCGGLCPGLNDVIRAIVRCLWGRYGVKRISGIRF
HHHHHHHCCCCCEEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCC
GYKGLLPDYNFDILPLTPEVIDNCHKTGGSLLGTSRGGGNRVVDIVDGIERLNLHILFII
CCCCCCCCCCCEEEECCHHHHHHHHHCCCCEEECCCCCCCEEEHHHHHHHCCCEEEEEEE
GGDGSQKGAKEIADEIKHRNLKISIIGIPKTVDNDISFVQKSFGFDTAIVKATEAVAAAH
CCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
MEARSQINGIGLVKLMGRESGFIATYTAIASHETNFVLIPEVSFDLDGPNGLLAHLEKRI
HHHHHHCCCCEEEEEECCCCCEEEEEEEEECCCCCEEEEECEEECCCCCCHHHHHHHHHH
ALRKHAVLVVAEGAGQDLMVNADGVPSGDSQGGSLRVSSGTDASGNKRLADIGLFLKEKI
HHHEEEEEEEECCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHC
GVYFKEKRIHINLKYIDPSYLIRSAVAAPIDSIYCERLGNNAVHAAMCGKTKMIIGLVHN
CEEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECC
KFVHLPIDVVVCQRKHVNPEGSLWRDALDATGQPIVMKNII
CEEECCEEEEEECCCCCCCCCHHHHHHHHCCCCCEEECCCC
>Mature Secondary Structure 
AHLPKEYDFSIESLGESKIPSPIYLSHTLGDFIPNYVSDNEYISHELSARLGETVGPFT
CCCCCCCCCCHHHCCCCCCCCCEEEHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHH
HKNLMERAGPRQKIFFNPHHVHAGIVTCGGLCPGLNDVIRAIVRCLWGRYGVKRISGIRF
HHHHHHHCCCCCEEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCC
GYKGLLPDYNFDILPLTPEVIDNCHKTGGSLLGTSRGGGNRVVDIVDGIERLNLHILFII
CCCCCCCCCCCEEEECCHHHHHHHHHCCCCEEECCCCCCCEEEHHHHHHHCCCEEEEEEE
GGDGSQKGAKEIADEIKHRNLKISIIGIPKTVDNDISFVQKSFGFDTAIVKATEAVAAAH
CCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
MEARSQINGIGLVKLMGRESGFIATYTAIASHETNFVLIPEVSFDLDGPNGLLAHLEKRI
HHHHHHCCCCEEEEEECCCCCEEEEEEEEECCCCCEEEEECEEECCCCCCHHHHHHHHHH
ALRKHAVLVVAEGAGQDLMVNADGVPSGDSQGGSLRVSSGTDASGNKRLADIGLFLKEKI
HHHEEEEEEEECCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHC
GVYFKEKRIHINLKYIDPSYLIRSAVAAPIDSIYCERLGNNAVHAAMCGKTKMIIGLVHN
CEEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECC
KFVHLPIDVVVCQRKHVNPEGSLWRDALDATGQPIVMKNII
CEEECCEEEEEECCCCCCCCCHHHHHHHHCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10913106 [H]