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The map label for this gene is cap [H]

Identifier: 189025311

GI number: 189025311

Start: 84251

End: 85867

Strand: Direct

Name: cap [H]

Synonym: TPASS_0077

Alternate gene names: 189025311

Gene position: 84251-85867 (Clockwise)

Preceding gene: 189025310

Following gene: 189025312

Centisome position: 7.39

GC content: 50.22

Gene sequence:

>1617_bases
ATGTGCGGCGCATCCCTGGGAGACTGCGTCGGGTCGTGTGCGCGTGTCAATGCGTTGTATGTAGGGAGAGATGGGGTGGG
TGCAGAAAATGTATGGGGCTGTGGTAGGTCTGCGGTGAGAGAGAGTGCGCACCGGGATGGCATCTACATCGTTGGCGCAG
GATTCGCAGGGAGTGTCCTTGCCCGTGAGATCCAAACGAAAAAAGTACTCGGCACAGTTATTGCTTTTTTGGATGACGAT
CCGTGCAAAATCGGATCGAATCTTCACGGTGTCCCGGTGCTTGGTCCCATTTTTGAAGTTGCCCGGATTGTGCGTATTAC
TCCGCATGATCACGCGCTGATTGCAATTCCTTCTATCTCCATTGAGCGTTTGCGTGACATTTACCTGACGCTGCGCGCTG
CGGGGTTTACGGTTATCAAACTTCTGCCGGCGCTTGCTCAAATCATCGATGGTACTGCGCATTTAGTGCAAACACGTGAA
ATTGATCCGCAGGATTTACTCACGCGCACACCGGTGACCATTAATCTTAAAAAGAGCCTGGCGTACCTATGCGGGAAACG
CGTGTTAGTTACTGGTGCTGGAGGATCTATCGGCAGCGAACTAGCCTGTCAGTTGCTCTCTGGTGATGTGGAGCGTCTCT
ATTTGTTCGGTCATGGAGAGAACTCCATCTACCAAATCGATAAAAAAATTCGTAGGCTGCAGGAAGAGGGTGTTGGATCT
CGCACAGTTATTGTTCCTGTTATTGGCGATTTAAAGGATGCTGCGTACGTGCGCTATCTTATCGAGCAGCTGCGCTGCGA
TGCGGTTTTTCATGCTGCCGCGTATAAGCACGTTCCTATGATGGAACTCAATCCTGTTTCAGTGATTGAAAATAATGTCT
TCGGCACCAAATTCTTGCTCGATGCCTGTATTGCGTGTAGGGTTAAGCGCTTTGTACTTTTGTCCACTGACAAGGCCGTG
GATCCTGTTTCTATCTACGGAGTATCTAAGATGCTCAACGAGAAGAATGTCTTGTATGCTGCTGAGCGTGTGCGCGATTT
CGGTCACGATGCCGCGTATATGTTTGTCCGTTTTGGAAACGTATTGGGTTCCCGTGGTTCTATCATGCCGCTCTTTATTG
AACAAATAAAGAAAGGGGGGCCCGTTACCGTGACAGATCCTGCCATGACACGATTCTTTATGACTATTCCCGAAGCGTGT
TCACTCGTTTTGCAAGTCGGTGGAGTAGGAGTAAATGGAGCGTCGTATCTTTTGGACATGGGGGAGCCTGTGAGCATTAT
GGAGACTGCGCAGCAACTTATTCGCTATTTTGGTTACGAGCCAGACAGAGATATTCCTATCCACGTGGTAGGCTTGCGTC
CTGGCGAGCGTCTCAGTGAGCCACTCGTTTCCAAAGACGAGCGTATAGAGCCGACGGTATATCCAAAGGTTCTGCGTTTG
CGTGAACGTGAACCTTTGGATTTTGCGCACCTTGAACGCCTGTGGGATCAACTGTATCCTTACTGTTTCCCTTCAGGAGA
AAAGGTGCGGTACCGGCACAAAGAAGGACTTGTCCGCGTGCTATGCGACTCGTGCGCGACACTGAAACAGCGGTATATGC
CAAATAGCGAGGCATAG

Upstream 100 bases:

>100_bases
TGTGTTTTTACCTTTGCGGCGGCGAAGTACTTGGTGGTGGGTTTGACCGCAGTGCGGTAAAGGGATAAACACTCTGCGCG
GGTGAGTACGTGCAGCAGAT

Downstream 100 bases:

>100_bases
GAAAATGGAAGGTACCGTGAAAAAAAAGAAAGAGGGTGTTCGTGATGATAACGCGCAGCATGCGGTGTTCAACAAACAAG
TGCCGTTTTTTGTGCCCTCG

Product: capsular polysaccharide biosynthesis protein

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MCGASLGDCVGSCARVNALYVGRDGVGAENVWGCGRSAVRESAHRDGIYIVGAGFAGSVLAREIQTKKVLGTVIAFLDDD
PCKIGSNLHGVPVLGPIFEVARIVRITPHDHALIAIPSISIERLRDIYLTLRAAGFTVIKLLPALAQIIDGTAHLVQTRE
IDPQDLLTRTPVTINLKKSLAYLCGKRVLVTGAGGSIGSELACQLLSGDVERLYLFGHGENSIYQIDKKIRRLQEEGVGS
RTVIVPVIGDLKDAAYVRYLIEQLRCDAVFHAAAYKHVPMMELNPVSVIENNVFGTKFLLDACIACRVKRFVLLSTDKAV
DPVSIYGVSKMLNEKNVLYAAERVRDFGHDAAYMFVRFGNVLGSRGSIMPLFIEQIKKGGPVTVTDPAMTRFFMTIPEAC
SLVLQVGGVGVNGASYLLDMGEPVSIMETAQQLIRYFGYEPDRDIPIHVVGLRPGERLSEPLVSKDERIEPTVYPKVLRL
REREPLDFAHLERLWDQLYPYCFPSGEKVRYRHKEGLVRVLCDSCATLKQRYMPNSEA

Sequences:

>Translated_538_residues
MCGASLGDCVGSCARVNALYVGRDGVGAENVWGCGRSAVRESAHRDGIYIVGAGFAGSVLAREIQTKKVLGTVIAFLDDD
PCKIGSNLHGVPVLGPIFEVARIVRITPHDHALIAIPSISIERLRDIYLTLRAAGFTVIKLLPALAQIIDGTAHLVQTRE
IDPQDLLTRTPVTINLKKSLAYLCGKRVLVTGAGGSIGSELACQLLSGDVERLYLFGHGENSIYQIDKKIRRLQEEGVGS
RTVIVPVIGDLKDAAYVRYLIEQLRCDAVFHAAAYKHVPMMELNPVSVIENNVFGTKFLLDACIACRVKRFVLLSTDKAV
DPVSIYGVSKMLNEKNVLYAAERVRDFGHDAAYMFVRFGNVLGSRGSIMPLFIEQIKKGGPVTVTDPAMTRFFMTIPEAC
SLVLQVGGVGVNGASYLLDMGEPVSIMETAQQLIRYFGYEPDRDIPIHVVGLRPGERLSEPLVSKDERIEPTVYPKVLRL
REREPLDFAHLERLWDQLYPYCFPSGEKVRYRHKEGLVRVLCDSCATLKQRYMPNSEA
>Mature_538_residues
MCGASLGDCVGSCARVNALYVGRDGVGAENVWGCGRSAVRESAHRDGIYIVGAGFAGSVLAREIQTKKVLGTVIAFLDDD
PCKIGSNLHGVPVLGPIFEVARIVRITPHDHALIAIPSISIERLRDIYLTLRAAGFTVIKLLPALAQIIDGTAHLVQTRE
IDPQDLLTRTPVTINLKKSLAYLCGKRVLVTGAGGSIGSELACQLLSGDVERLYLFGHGENSIYQIDKKIRRLQEEGVGS
RTVIVPVIGDLKDAAYVRYLIEQLRCDAVFHAAAYKHVPMMELNPVSVIENNVFGTKFLLDACIACRVKRFVLLSTDKAV
DPVSIYGVSKMLNEKNVLYAAERVRDFGHDAAYMFVRFGNVLGSRGSIMPLFIEQIKKGGPVTVTDPAMTRFFMTIPEAC
SLVLQVGGVGVNGASYLLDMGEPVSIMETAQQLIRYFGYEPDRDIPIHVVGLRPGERLSEPLVSKDERIEPTVYPKVLRL
REREPLDFAHLERLWDQLYPYCFPSGEKVRYRHKEGLVRVLCDSCATLKQRYMPNSEA

Specific function: Involved in biofilm formation [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 59306; Mature: 59306

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGASLGDCVGSCARVNALYVGRDGVGAENVWGCGRSAVRESAHRDGIYIVGAGFAGSVL
CCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHH
AREIQTKKVLGTVIAFLDDDPCKIGSNLHGVPVLGPIFEVARIVRITPHDHALIAIPSIS
HHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHHHHHHHEEECCCCCEEEEECCCC
IERLRDIYLTLRAAGFTVIKLLPALAQIIDGTAHLVQTREIDPQDLLTRTPVTINLKKSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCHHHHHHCCCEEEEHHHHH
AYLCGKRVLVTGAGGSIGSELACQLLSGDVERLYLFGHGENSIYQIDKKIRRLQEEGVGS
HHHCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCC
RTVIVPVIGDLKDAAYVRYLIEQLRCDAVFHAAAYKHVPMMELNPVSVIENNVFGTKFLL
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHH
DACIACRVKRFVLLSTDKAVDPVSIYGVSKMLNEKNVLYAAERVRDFGHDAAYMFVRFGN
HHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHH
VLGSRGSIMPLFIEQIKKGGPVTVTDPAMTRFFMTIPEACSLVLQVGGVGVNGASYLLDM
HHCCCCCCHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEC
GEPVSIMETAQQLIRYFGYEPDRDIPIHVVGLRPGERLSEPLVSKDERIEPTVYPKVLRL
CCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCCCCHHHHHHHH
REREPLDFAHLERLWDQLYPYCFPSGEKVRYRHKEGLVRVLCDSCATLKQRYMPNSEA
HCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MCGASLGDCVGSCARVNALYVGRDGVGAENVWGCGRSAVRESAHRDGIYIVGAGFAGSVL
CCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHH
AREIQTKKVLGTVIAFLDDDPCKIGSNLHGVPVLGPIFEVARIVRITPHDHALIAIPSIS
HHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHHHHHHHEEECCCCCEEEEECCCC
IERLRDIYLTLRAAGFTVIKLLPALAQIIDGTAHLVQTREIDPQDLLTRTPVTINLKKSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCHHHHHHCCCEEEEHHHHH
AYLCGKRVLVTGAGGSIGSELACQLLSGDVERLYLFGHGENSIYQIDKKIRRLQEEGVGS
HHHCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCC
RTVIVPVIGDLKDAAYVRYLIEQLRCDAVFHAAAYKHVPMMELNPVSVIENNVFGTKFLL
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHH
DACIACRVKRFVLLSTDKAVDPVSIYGVSKMLNEKNVLYAAERVRDFGHDAAYMFVRFGN
HHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHH
VLGSRGSIMPLFIEQIKKGGPVTVTDPAMTRFFMTIPEACSLVLQVGGVGVNGASYLLDM
HHCCCCCCHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEC
GEPVSIMETAQQLIRYFGYEPDRDIPIHVVGLRPGERLSEPLVSKDERIEPTVYPKVLRL
CCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCCCCHHHHHHHH
REREPLDFAHLERLWDQLYPYCFPSGEKVRYRHKEGLVRVLCDSCATLKQRYMPNSEA
HCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]