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The map label for this gene is pbpG [H]

Identifier: 188587475

GI number: 188587475

Start: 3114515

End: 3116632

Strand: Reverse

Name: pbpG [H]

Synonym: Nther_2885

Alternate gene names: 188587475

Gene position: 3116632-3114515 (Counterclockwise)

Preceding gene: 188587478

Following gene: 188587472

Centisome position: 98.45

GC content: 39.66

Gene sequence:

>2118_bases
TTGAGGCGCAAAAGATCGCCATCAAAAGAACAATACCTAGAGAACAAAAAAAGACCGGGCTTTATTAATATATTTAAAAA
AATTTTCAAATTGCTGTTTATTGTAGCAATTATTTCTGTCATTGGATTTATTATCTTATTACCAGTTGTAGTATCTGCTT
TAGAAGATGTCTCGCCTCCTGAATTTGAAAGTGCTACTGTTATGTTAGATAAAAATGGAGAGGAATTCTATCATGAGTAC
GATGATTATCGGATTTATATTTCAATAGATGAAATGCCAGAAGAAATGGTAGAAGGGATCATGGCCGTAGAAGACAGGAG
GTTCAAGGAACACTTAGGAATAGATATAAGAGGTATAACTAGGGCTATGTGGCGTAATATTAGAGCCAGAGAAGTCGTCG
AGGGTGGTAGTACTATTACCCAACAATTAGCTAAAAATTACTTTTTAGATCATTCCAGGACAATAGATAGAAAAGTTCGA
GAATTTTTCTTGACTTTGCATCTAGAAAGGACATTGAGTAAAGAGGAAATTTTGGAACAGTATTTAAACGGTATCTATTT
CGGCCATGGAGCTTATGGTGTAGAAGCTGCCAGTAGAATGTATTTTGGAAAAAATACCAATGAATTAGAGCTTCAAGAGA
TTGCTATGTTAATTGGAATACCGAGAAGTCCCTATTATTATTCACCATTTCGAAATCCAGAAAATGCTCAACAAAGACGT
GATGCAGTCTTACATATAATGGCTCAAGAAGGAGTGGTTAATCAGGACACAGCTCAAGCAGCTGCTGTTGAAGCACTAGA
TGTTACTGGATATAATCCTCCCAAGGAAAATGCTTCTTATTTTGCAGATGAGGTTAACAGGCGACTAGAAGAGAAATTGC
CAGAAGATTATACAGAAGATTTTCAAAGGGGAGGCTTTCGTGTATATACTACTTTAGATCAGGAAATGCAGTCAGAAGCT
GAAAGAATTTTAGATGAAGACTTGGGAGTAGTCAGATATGCTGAAAGAGGGGATTCGGAAATTCGTCAACCACAAGGTTC
TATGGTAGCATTGGAGCCTGATACCGGTAGGGTGAGAGCTTTCGTAGGTGGTCGGGACTATGAAGAAACCCCTTTCAATC
GAGCTAATCCTATTAGTCCTAATAGACCAGTTGGTTCTACGATTAAACCATTTGTATATGCTGCAGCTTTATCGGAAAAT
GAAATCACACCTGCTACTACCTTTACTTGTGAGGAAACTGTATACCCAGAGGAACCGGGATGGGATAATGGCCCCTTCGT
ACCTACTGATTATGGCGGTGTTTACCATAATGATGACCTGACAATTCGCAGAGCAATTCGGGAATCGTGTAATGTAACTG
CTGTTAAAGTTCATGATGATATGGGGCGGTATCCTAGTGTGGAGCAGGCTGAAAGTATGGGAGTAATTGACGATATTCCA
ACTACCCTTTCCTTTCCTCTAGGACAGGTCGAAGCTTCGCCTTTAAAACTTACATCAGGATATACACCTTTTGCCAATGA
AGGAATTTTGGTAGAACCGAAACTGATAAAAAAGGTTGAAGATAGTAGGGGCACAGTTATCTATGAAAATGAGCCAGAGC
GGTCAGTAGAGCTGAGTCAACAAACAGCTTATCAAATGACCAGTATAATGGAGGATGTGATTTCTCCTAGAGGCACGGCT
TCAAGTATATGGGATGAAATTGATTTTCCAGCTGCCGGGAAAACAGGCACAACGGAAGGCCGAATGGATGCAGTTTTTGT
AGGTTATACCCCCGAACTAGTAACTACTGTATTTGTAAGTGATGATGGCAATGTAGAACCCGTTCCCGGAACTGGAGGGA
GTTTAGCTGCACCTCTTTGGAGTGAATTTATGAATTCTGTTTCTCATAAATTGGATCATGAAGACTTTTCTCGTCCTGAT
GGACTAATTGAACGTGAAATCTGTGAAGATTCGGGGAAACTAGCAACAGATTATTGTCCTGAAGAAGTAGTAACAACTGA
ATATTTTGAACCGAGAACCCAACCCGATAATGATTGTGATGTACATTTACCAGAAAAAGAGGAAGGCCCTGGCTTTCCTC
CTCCTTGGTGGCCATTTTTTCAAGAAGATAACGATTAG

Upstream 100 bases:

>100_bases
TTGCGGAACAGGGAGAATCCATTGAATAATTGGATAACAACCTCAAATTCTGGGAAGACTTATATAGATAGAAAAATTAT
TAGGAAAAGAGGGAATTATT

Downstream 100 bases:

>100_bases
TTCTATAGGGCGATGAAAAAGTTTACCAGGATTGGCACTAAAATGGACAATATGGCTCAATTAAAAAAAGCAATTGGTAT
ATATTGTTTGCCTGTTCCCT

Product: penicillin-binding protein, 1A family

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MRRKRSPSKEQYLENKKRPGFINIFKKIFKLLFIVAIISVIGFIILLPVVVSALEDVSPPEFESATVMLDKNGEEFYHEY
DDYRIYISIDEMPEEMVEGIMAVEDRRFKEHLGIDIRGITRAMWRNIRAREVVEGGSTITQQLAKNYFLDHSRTIDRKVR
EFFLTLHLERTLSKEEILEQYLNGIYFGHGAYGVEAASRMYFGKNTNELELQEIAMLIGIPRSPYYYSPFRNPENAQQRR
DAVLHIMAQEGVVNQDTAQAAAVEALDVTGYNPPKENASYFADEVNRRLEEKLPEDYTEDFQRGGFRVYTTLDQEMQSEA
ERILDEDLGVVRYAERGDSEIRQPQGSMVALEPDTGRVRAFVGGRDYEETPFNRANPISPNRPVGSTIKPFVYAAALSEN
EITPATTFTCEETVYPEEPGWDNGPFVPTDYGGVYHNDDLTIRRAIRESCNVTAVKVHDDMGRYPSVEQAESMGVIDDIP
TTLSFPLGQVEASPLKLTSGYTPFANEGILVEPKLIKKVEDSRGTVIYENEPERSVELSQQTAYQMTSIMEDVISPRGTA
SSIWDEIDFPAAGKTGTTEGRMDAVFVGYTPELVTTVFVSDDGNVEPVPGTGGSLAAPLWSEFMNSVSHKLDHEDFSRPD
GLIEREICEDSGKLATDYCPEEVVTTEYFEPRTQPDNDCDVHLPEKEEGPGFPPPWWPFFQEDND

Sequences:

>Translated_705_residues
MRRKRSPSKEQYLENKKRPGFINIFKKIFKLLFIVAIISVIGFIILLPVVVSALEDVSPPEFESATVMLDKNGEEFYHEY
DDYRIYISIDEMPEEMVEGIMAVEDRRFKEHLGIDIRGITRAMWRNIRAREVVEGGSTITQQLAKNYFLDHSRTIDRKVR
EFFLTLHLERTLSKEEILEQYLNGIYFGHGAYGVEAASRMYFGKNTNELELQEIAMLIGIPRSPYYYSPFRNPENAQQRR
DAVLHIMAQEGVVNQDTAQAAAVEALDVTGYNPPKENASYFADEVNRRLEEKLPEDYTEDFQRGGFRVYTTLDQEMQSEA
ERILDEDLGVVRYAERGDSEIRQPQGSMVALEPDTGRVRAFVGGRDYEETPFNRANPISPNRPVGSTIKPFVYAAALSEN
EITPATTFTCEETVYPEEPGWDNGPFVPTDYGGVYHNDDLTIRRAIRESCNVTAVKVHDDMGRYPSVEQAESMGVIDDIP
TTLSFPLGQVEASPLKLTSGYTPFANEGILVEPKLIKKVEDSRGTVIYENEPERSVELSQQTAYQMTSIMEDVISPRGTA
SSIWDEIDFPAAGKTGTTEGRMDAVFVGYTPELVTTVFVSDDGNVEPVPGTGGSLAAPLWSEFMNSVSHKLDHEDFSRPD
GLIEREICEDSGKLATDYCPEEVVTTEYFEPRTQPDNDCDVHLPEKEEGPGFPPPWWPFFQEDND
>Mature_705_residues
MRRKRSPSKEQYLENKKRPGFINIFKKIFKLLFIVAIISVIGFIILLPVVVSALEDVSPPEFESATVMLDKNGEEFYHEY
DDYRIYISIDEMPEEMVEGIMAVEDRRFKEHLGIDIRGITRAMWRNIRAREVVEGGSTITQQLAKNYFLDHSRTIDRKVR
EFFLTLHLERTLSKEEILEQYLNGIYFGHGAYGVEAASRMYFGKNTNELELQEIAMLIGIPRSPYYYSPFRNPENAQQRR
DAVLHIMAQEGVVNQDTAQAAAVEALDVTGYNPPKENASYFADEVNRRLEEKLPEDYTEDFQRGGFRVYTTLDQEMQSEA
ERILDEDLGVVRYAERGDSEIRQPQGSMVALEPDTGRVRAFVGGRDYEETPFNRANPISPNRPVGSTIKPFVYAAALSEN
EITPATTFTCEETVYPEEPGWDNGPFVPTDYGGVYHNDDLTIRRAIRESCNVTAVKVHDDMGRYPSVEQAESMGVIDDIP
TTLSFPLGQVEASPLKLTSGYTPFANEGILVEPKLIKKVEDSRGTVIYENEPERSVELSQQTAYQMTSIMEDVISPRGTA
SSIWDEIDFPAAGKTGTTEGRMDAVFVGYTPELVTTVFVSDDGNVEPVPGTGGSLAAPLWSEFMNSVSHKLDHEDFSRPD
GLIEREICEDSGKLATDYCPEEVVTTEYFEPRTQPDNDCDVHLPEKEEGPGFPPPWWPFFQEDND

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=578, Percent_Identity=28.8927335640138, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87082258, Length=330, Percent_Identity=35.1515151515151, Blast_Score=192, Evalue=6e-50,
Organism=Escherichia coli, GI1788867, Length=649, Percent_Identity=27.5808936825886, Blast_Score=177, Evalue=3e-45,
Organism=Escherichia coli, GI1789601, Length=219, Percent_Identity=34.2465753424658, Blast_Score=113, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 79814; Mature: 79814

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKRSPSKEQYLENKKRPGFINIFKKIFKLLFIVAIISVIGFIILLPVVVSALEDVSPP
CCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EFESATVMLDKNGEEFYHEYDDYRIYISIDEMPEEMVEGIMAVEDRRFKEHLGIDIRGIT
CCCCEEEEEECCCHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RAMWRNIRAREVVEGGSTITQQLAKNYFLDHSRTIDRKVREFFLTLHLERTLSKEEILEQ
HHHHHCCHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YLNGIYFGHGAYGVEAASRMYFGKNTNELELQEIAMLIGIPRSPYYYSPFRNPENAQQRR
HHCCEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHH
DAVLHIMAQEGVVNQDTAQAAAVEALDVTGYNPPKENASYFADEVNRRLEEKLPEDYTED
HHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
FQRGGFRVYTTLDQEMQSEAERILDEDLGVVRYAERGDSEIRQPQGSMVALEPDTGRVRA
HHCCCEEEEEEHHHHHHHHHHHHHHHCCCCEEEHHCCCHHHHCCCCCEEEECCCCCCEEE
FVGGRDYEETPFNRANPISPNRPVGSTIKPFVYAAALSENEITPATTFTCEETVYPEEPG
EECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCC
WDNGPFVPTDYGGVYHNDDLTIRRAIRESCNVTAVKVHDDMGRYPSVEQAESMGVIDDIP
CCCCCCCCCCCCCEEECCCHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHCCCHHCCC
TTLSFPLGQVEASPLKLTSGYTPFANEGILVEPKLIKKVEDSRGTVIYENEPERSVELSQ
CHHCCCCCCCCCCCEEEECCCCCCCCCCEEECHHHHHHHHCCCCEEEECCCCCCHHHHHH
QTAYQMTSIMEDVISPRGTASSIWDEIDFPAAGKTGTTEGRMDAVFVGYTPELVTTVFVS
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEC
DDGNVEPVPGTGGSLAAPLWSEFMNSVSHKLDHEDFSRPDGLIEREICEDSGKLATDYCP
CCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHCCCCCCCCCCC
EEVVTTEYFEPRTQPDNDCDVHLPEKEEGPGFPPPWWPFFQEDND
HHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRRKRSPSKEQYLENKKRPGFINIFKKIFKLLFIVAIISVIGFIILLPVVVSALEDVSPP
CCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EFESATVMLDKNGEEFYHEYDDYRIYISIDEMPEEMVEGIMAVEDRRFKEHLGIDIRGIT
CCCCEEEEEECCCHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RAMWRNIRAREVVEGGSTITQQLAKNYFLDHSRTIDRKVREFFLTLHLERTLSKEEILEQ
HHHHHCCHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YLNGIYFGHGAYGVEAASRMYFGKNTNELELQEIAMLIGIPRSPYYYSPFRNPENAQQRR
HHCCEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHH
DAVLHIMAQEGVVNQDTAQAAAVEALDVTGYNPPKENASYFADEVNRRLEEKLPEDYTED
HHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
FQRGGFRVYTTLDQEMQSEAERILDEDLGVVRYAERGDSEIRQPQGSMVALEPDTGRVRA
HHCCCEEEEEEHHHHHHHHHHHHHHHCCCCEEEHHCCCHHHHCCCCCEEEECCCCCCEEE
FVGGRDYEETPFNRANPISPNRPVGSTIKPFVYAAALSENEITPATTFTCEETVYPEEPG
EECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCC
WDNGPFVPTDYGGVYHNDDLTIRRAIRESCNVTAVKVHDDMGRYPSVEQAESMGVIDDIP
CCCCCCCCCCCCCEEECCCHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHCCCHHCCC
TTLSFPLGQVEASPLKLTSGYTPFANEGILVEPKLIKKVEDSRGTVIYENEPERSVELSQ
CHHCCCCCCCCCCCEEEECCCCCCCCCCEEECHHHHHHHHCCCCEEEECCCCCCHHHHHH
QTAYQMTSIMEDVISPRGTASSIWDEIDFPAAGKTGTTEGRMDAVFVGYTPELVTTVFVS
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEC
DDGNVEPVPGTGGSLAAPLWSEFMNSVSHKLDHEDFSRPDGLIEREICEDSGKLATDYCP
CCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHCCCCCCCCCCC
EEVVTTEYFEPRTQPDNDCDVHLPEKEEGPGFPPPWWPFFQEDND
HHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]