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The map label for this gene is sigG [H]

Identifier: 188587460

GI number: 188587460

Start: 3101713

End: 3102534

Strand: Reverse

Name: sigG [H]

Synonym: Nther_2870

Alternate gene names: 188587460

Gene position: 3102534-3101713 (Counterclockwise)

Preceding gene: 188587461

Following gene: 188587459

Centisome position: 98.01

GC content: 37.96

Gene sequence:

>822_bases
ATGAAGTTACAAAGAGTTACTCTGAGCGGTATAAATACATACAAATTACCAGTTATTTCATCTGAAGAAACTCAAAAACT
TTTTGAGAGGTTAGAGCAGGGAGATGAACAAGCCAAACAAGAGTTAATTAACGGTAATTTGCGACTGGTATTGAGCGTGA
TTAATAAGTTTAAAAATCGGGGAGAAAACCTGGACGACTTATTTCAAGTAGGTTGTTTAGGTTTAGTTAAAGCCGTGGAT
AATTTTGACCCCAAACAAGGTGTTAAATTTTCTACTTATGCTGTTCCTATGATTATGGGAGAAATAAAAAGATATCTAAG
AGACGATAGTCCCATGAGAGTCAGTAGAAGTTTGAAATTGCTTGGTCAAGAGGCGGCGAAAGCCAAAGAAAAGCTGCGAA
AGGAAAAACCTTATGAACCTACAATAGAAGAACTTGCCAGTGAATTGAATATTAGCCGAGAAGAGCTTGTGGAGGCTTTG
AACTCTTCTTCTGAACCTTTAAGTTTATATGATCCCGTTTATTCGGATAGCACAGACCCCGTGCTGTTGGTAGATCAAAT
TAAAGACGAAGAAGAACGACTTGAAAACTGGGTCGAAAATATGACTTTAAAAGAAGCCATTGCTAAGCTTCCGGAGAGAC
AAAGGAATATTGTCAACCGCAGATTTTTTAAAGGACAAACCCAGGTAGAAGTTTCAGATGAGTTAAATATTTCTCAGGCC
CAGGTTTCTCGTTTGGAGAAATCAGCTTTATTACAGTTGAGAAAACTAGTTAAAGAAGAAGTGGCAGCTAATCGGGAGGG
GAGGTCGAATCAAGATGATTAA

Upstream 100 bases:

>100_bases
TATGTCTAATTTTTGAAAAAACTGGCAATACTCTGGTATAGAGGCGCTTTTGAGCCGGCTTAAAAGCCCTTGCCTTTTTG
TTAATAGGGAGGGTTGTATA

Downstream 100 bases:

>100_bases
TATGAACAAATTTATTAATAAATTCAGGGAGTTAACAGACCGTACAAAACTATTAATCATTACGCACAAGATAACTAAGA
GAACATTTCTAATTATATTT

Product: sporulation sigma factor SigG

Products: NA

Alternate protein names: Stage III sporulation protein G [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKLQRVTLSGINTYKLPVISSEETQKLFERLEQGDEQAKQELINGNLRLVLSVINKFKNRGENLDDLFQVGCLGLVKAVD
NFDPKQGVKFSTYAVPMIMGEIKRYLRDDSPMRVSRSLKLLGQEAAKAKEKLRKEKPYEPTIEELASELNISREELVEAL
NSSSEPLSLYDPVYSDSTDPVLLVDQIKDEEERLENWVENMTLKEAIAKLPERQRNIVNRRFFKGQTQVEVSDELNISQA
QVSRLEKSALLQLRKLVKEEVAANREGRSNQDD

Sequences:

>Translated_273_residues
MKLQRVTLSGINTYKLPVISSEETQKLFERLEQGDEQAKQELINGNLRLVLSVINKFKNRGENLDDLFQVGCLGLVKAVD
NFDPKQGVKFSTYAVPMIMGEIKRYLRDDSPMRVSRSLKLLGQEAAKAKEKLRKEKPYEPTIEELASELNISREELVEAL
NSSSEPLSLYDPVYSDSTDPVLLVDQIKDEEERLENWVENMTLKEAIAKLPERQRNIVNRRFFKGQTQVEVSDELNISQA
QVSRLEKSALLQLRKLVKEEVAANREGRSNQDD
>Mature_273_residues
MKLQRVTLSGINTYKLPVISSEETQKLFERLEQGDEQAKQELINGNLRLVLSVINKFKNRGENLDDLFQVGCLGLVKAVD
NFDPKQGVKFSTYAVPMIMGEIKRYLRDDSPMRVSRSLKLLGQEAAKAKEKLRKEKPYEPTIEELASELNISREELVEAL
NSSSEPLSLYDPVYSDSTDPVLLVDQIKDEEERLENWVENMTLKEAIAKLPERQRNIVNRRFFKGQTQVEVSDELNISQA
QVSRLEKSALLQLRKLVKEEVAANREGRSNQDD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes in the forespore [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=256, Percent_Identity=28.90625, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1789448, Length=250, Percent_Identity=28.4, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1789871, Length=270, Percent_Identity=29.6296296296296, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788231, Length=195, Percent_Identity=30.7692307692308, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014212
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 31254; Mature: 31254

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQRVTLSGINTYKLPVISSEETQKLFERLEQGDEQAKQELINGNLRLVLSVINKFKNR
CCCCEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GENLDDLFQVGCLGLVKAVDNFDPKQGVKFSTYAVPMIMGEIKRYLRDDSPMRVSRSLKL
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LGQEAAKAKEKLRKEKPYEPTIEELASELNISREELVEALNSSSEPLSLYDPVYSDSTDP
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHCCCCCCCCCC
VLLVDQIKDEEERLENWVENMTLKEAIAKLPERQRNIVNRRFFKGQTQVEVSDELNISQA
EEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHH
QVSRLEKSALLQLRKLVKEEVAANREGRSNQDD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKLQRVTLSGINTYKLPVISSEETQKLFERLEQGDEQAKQELINGNLRLVLSVINKFKNR
CCCCEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GENLDDLFQVGCLGLVKAVDNFDPKQGVKFSTYAVPMIMGEIKRYLRDDSPMRVSRSLKL
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LGQEAAKAKEKLRKEKPYEPTIEELASELNISREELVEALNSSSEPLSLYDPVYSDSTDP
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHCCCCCCCCCC
VLLVDQIKDEEERLENWVENMTLKEAIAKLPERQRNIVNRRFFKGQTQVEVSDELNISQA
EEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHH
QVSRLEKSALLQLRKLVKEEVAANREGRSNQDD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2497052; 2459711; 9384377 [H]