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The map label for this gene is hutU

Identifier: 188587410

GI number: 188587410

Start: 3048300

End: 3049949

Strand: Reverse

Name: hutU

Synonym: Nther_2819

Alternate gene names: 188587410

Gene position: 3049949-3048300 (Counterclockwise)

Preceding gene: 188587411

Following gene: 188587409

Centisome position: 96.35

GC content: 42.3

Gene sequence:

>1650_bases
ATGTCAAGGAAGGTTAGAGCACCCAGAGGAACTGATTTGCATTGTAAAGGTTGGCAGCAGGAAGGGCCTATGAGAATGTT
AATGAACAACCTTGACCCAGAAGTTGCTGAAAAGCCTGAAGAACTTATTGTTTATGGTGGTAGTGGCAAGGCAGCAAGAA
ACTGGGAAGCCTTTGATGCCATTGTAAAAAGTTTACAAGAACTTGAGGATGATGAAACTCTACTAGTACAATCGGGAAAA
CCTGTGGGAGTATTTAAAACCCATTCTCAAGCACCTCGGGTGCTAATTGCAAATTCACTGTTGGTTCCATCCTGGGCAGA
TTGGGATCAATTCAGGGAACTAGAGAAACAGGGTTTGACCATGTATGGACAAATGACTGCGGGTAGTTGGATTTATATTG
GCACCCAGGGAATATTACAGGGTACCTATGAAACATTTGGAGCGGCTGCTGATAAACATTACAATGGAGATTTGTCCGGA
AAGTTTGTACTAACTGCTGGCCTTGGAGGAATGGGGGGTGCCCAACCTTTAGCTGTGACCATGAATAATGGTGTGGTATT
ATGCGTAGAAGTTGACAGGGAACGTATAGAAAGAAGAATTGATTATCAATATTTAGATAAAATGGCAGAAAATTTGGACG
AAGCGTTAGAGTTAGTGGATGAAGCCGTCTCCGAGAAAAAACCCCTTTCTATTGGGCTTTTAGGGAACGCAGCTTCTATT
TATCCGGAATTAGTTAAAAGGGGTAGAATTCCGGATATGGTAACTGACCAAACATCGGCTCACGACATCCTAAATGGGTA
TGTACCAGCAGATTTAGACTTTCCTTATGCTTTAGAGCTCAGACATCAAGATCCAGAAAAATACATGAAGCTTTCAAAAT
CAAGTATAGCGAAACATGTGGAAGCCATGCTTGAATTTCAAAAACAAGGAGCTATAGTTTTTGACTATGGGAACAATATC
CGACAGCAAGCTTACATTGAAGGTATTACTAAAGCTTTTGATTTTCCCGGTTTTGTTCCGGCTTATATACGTCCGCAATT
TTGTGAAGGCAAAGGGCCCTTCCGCTGGGTAGCTCTATCAGGGGATCCGGAAGATATATATCGTACAGATCGATTAATAT
TAGAAGAGTTTTCAGACAATGAACATTTATGTCGCTGGATTAAAATGGCTGGTGAACAAGTGAGCTTTCAGGGACTTCCC
TCCAGAATTTGTTGGTTAGGTTACGGTGAGAGAGCTCGATTTGGGAAGTTGATCAATGATCTAGTAGCTAAAGGTGAAAT
TAAAGCACCAATTGTTATTGGCCGAGACCACTTAGATTGTGGTTCAGTAGCTTCACCAAATAGAGAAACTGAAGGCATGA
AAGATGGCAGTGATGCCATTGCTGATTGGCCTATTTTGAATGCTTTAATTAATACGGCTGCTGGGGCTCACTGGGTATCG
GTACATCATGGCGGAGGTGTGGGAATAGGTTATTCCCTTCACGCTGGAATTGTAGTCATGGCAGATGGCAGTAAGGACGC
CGAAGAACGACTTGAACGAGTTTTAACTTCAGATCCTGGAACAGGTATTATTAGGCATGTAGATGCCGGTTATGAACTAG
CTAAAAAAACTGCCAGTGAGCAGGGAGTTAATATACCCATGTGGAGCTAA

Upstream 100 bases:

>100_bases
TTTTACTATTGTAAAGGCCGAATTTACTATAAAATTTCAGAAATCTTAAAATAGTTTAAAATTAAAGTAAATTATGATGC
TTGAGAAAGGAGTGGCGATT

Downstream 100 bases:

>100_bases
CTAGTTTTATTAATAAAAAATTAACTCATAAATATAGGAGGTGGTTTTACAAACTCAAGTATTTGGCTCATAGTGATTCC
ATTGTTTATTGGTTATTGGT

Product: urocanate hydratase

Products: NA

Alternate protein names: Urocanase; Imidazolonepropionate hydrolase

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MSRKVRAPRGTDLHCKGWQQEGPMRMLMNNLDPEVAEKPEELIVYGGSGKAARNWEAFDAIVKSLQELEDDETLLVQSGK
PVGVFKTHSQAPRVLIANSLLVPSWADWDQFRELEKQGLTMYGQMTAGSWIYIGTQGILQGTYETFGAAADKHYNGDLSG
KFVLTAGLGGMGGAQPLAVTMNNGVVLCVEVDRERIERRIDYQYLDKMAENLDEALELVDEAVSEKKPLSIGLLGNAASI
YPELVKRGRIPDMVTDQTSAHDILNGYVPADLDFPYALELRHQDPEKYMKLSKSSIAKHVEAMLEFQKQGAIVFDYGNNI
RQQAYIEGITKAFDFPGFVPAYIRPQFCEGKGPFRWVALSGDPEDIYRTDRLILEEFSDNEHLCRWIKMAGEQVSFQGLP
SRICWLGYGERARFGKLINDLVAKGEIKAPIVIGRDHLDCGSVASPNRETEGMKDGSDAIADWPILNALINTAAGAHWVS
VHHGGGVGIGYSLHAGIVVMADGSKDAEERLERVLTSDPGTGIIRHVDAGYELAKKTASEQGVNIPMWS

Sequences:

>Translated_549_residues
MSRKVRAPRGTDLHCKGWQQEGPMRMLMNNLDPEVAEKPEELIVYGGSGKAARNWEAFDAIVKSLQELEDDETLLVQSGK
PVGVFKTHSQAPRVLIANSLLVPSWADWDQFRELEKQGLTMYGQMTAGSWIYIGTQGILQGTYETFGAAADKHYNGDLSG
KFVLTAGLGGMGGAQPLAVTMNNGVVLCVEVDRERIERRIDYQYLDKMAENLDEALELVDEAVSEKKPLSIGLLGNAASI
YPELVKRGRIPDMVTDQTSAHDILNGYVPADLDFPYALELRHQDPEKYMKLSKSSIAKHVEAMLEFQKQGAIVFDYGNNI
RQQAYIEGITKAFDFPGFVPAYIRPQFCEGKGPFRWVALSGDPEDIYRTDRLILEEFSDNEHLCRWIKMAGEQVSFQGLP
SRICWLGYGERARFGKLINDLVAKGEIKAPIVIGRDHLDCGSVASPNRETEGMKDGSDAIADWPILNALINTAAGAHWVS
VHHGGGVGIGYSLHAGIVVMADGSKDAEERLERVLTSDPGTGIIRHVDAGYELAKKTASEQGVNIPMWS
>Mature_548_residues
SRKVRAPRGTDLHCKGWQQEGPMRMLMNNLDPEVAEKPEELIVYGGSGKAARNWEAFDAIVKSLQELEDDETLLVQSGKP
VGVFKTHSQAPRVLIANSLLVPSWADWDQFRELEKQGLTMYGQMTAGSWIYIGTQGILQGTYETFGAAADKHYNGDLSGK
FVLTAGLGGMGGAQPLAVTMNNGVVLCVEVDRERIERRIDYQYLDKMAENLDEALELVDEAVSEKKPLSIGLLGNAASIY
PELVKRGRIPDMVTDQTSAHDILNGYVPADLDFPYALELRHQDPEKYMKLSKSSIAKHVEAMLEFQKQGAIVFDYGNNIR
QQAYIEGITKAFDFPGFVPAYIRPQFCEGKGPFRWVALSGDPEDIYRTDRLILEEFSDNEHLCRWIKMAGEQVSFQGLPS
RICWLGYGERARFGKLINDLVAKGEIKAPIVIGRDHLDCGSVASPNRETEGMKDGSDAIADWPILNALINTAAGAHWVSV
HHGGGVGIGYSLHAGIVVMADGSKDAEERLERVLTSDPGTGIIRHVDAGYELAKKTASEQGVNIPMWS

Specific function: Unknown

COG id: COG2987

COG function: function code E; Urocanate hydratase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the urocanase family

Homologues:

Organism=Homo sapiens, GI21389467, Length=552, Percent_Identity=35.3260869565217, Blast_Score=327, Evalue=3e-89,
Organism=Homo sapiens, GI260306182, Length=354, Percent_Identity=32.7683615819209, Blast_Score=169, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI71997891, Length=547, Percent_Identity=33.8208409506399, Blast_Score=315, Evalue=4e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HUTU_NATTJ (B2A3D8)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001918955.1
- ProteinModelPortal:   B2A3D8
- SMR:   B2A3D8
- GeneID:   6316813
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_2819
- HOGENOM:   HBG305285
- OMA:   IRQMAFE
- ProtClustDB:   PRK05414
- GO:   GO:0005737
- HAMAP:   MF_00577
- InterPro:   IPR000193
- PIRSF:   PIRSF001423
- TIGRFAMs:   TIGR01228

Pfam domain/function: PF01175 Urocanase; SSF111326 Urocanase

EC number: =4.2.1.49

Molecular weight: Translated: 60588; Mature: 60457

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS01233 UROCANASE

Important sites: ACT_SITE 404-404

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKVRAPRGTDLHCKGWQQEGPMRMLMNNLDPEVAEKPEELIVYGGSGKAARNWEAFDA
CCCCCCCCCCCCCEECCCCCCCCHHHHHHCCCCHHHCCCCEEEEECCCCCCCCCHHHHHH
IVKSLQELEDDETLLVQSGKPVGVFKTHSQAPRVLIANSLLVPSWADWDQFRELEKQGLT
HHHHHHHCCCCCEEEEECCCCCEEEECCCCCCEEEEECCEECCCCCCHHHHHHHHHCCCE
MYGQMTAGSWIYIGTQGILQGTYETFGAAADKHYNGDLSGKFVLTAGLGGMGGAQPLAVT
EEEEECCCCEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEE
MNNGVVLCVEVDRERIERRIDYQYLDKMAENLDEALELVDEAVSEKKPLSIGLLGNAASI
ECCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHH
YPELVKRGRIPDMVTDQTSAHDILNGYVPADLDFPYALELRHQDPEKYMKLSKSSIAKHV
HHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
EAMLEFQKQGAIVFDYGNNIRQQAYIEGITKAFDFPGFVPAYIRPQFCEGKGPFRWVALS
HHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHCCCHHCCCCCCEEEEEEC
GDPEDIYRTDRLILEEFSDNEHLCRWIKMAGEQVSFQGLPSRICWLGYGERARFGKLIND
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEECCCCCCEEEEECCCCHHHHHHHHHH
LVAKGEIKAPIVIGRDHLDCGSVASPNRETEGMKDGSDAIADWPILNALINTAAGAHWVS
HHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
VHHGGGVGIGYSLHAGIVVMADGSKDAEERLERVLTSDPGTGIIRHVDAGYELAKKTASE
EECCCCCCCCEEECCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHH
QGVNIPMWS
CCCCCCCCC
>Mature Secondary Structure 
SRKVRAPRGTDLHCKGWQQEGPMRMLMNNLDPEVAEKPEELIVYGGSGKAARNWEAFDA
CCCCCCCCCCCCEECCCCCCCCHHHHHHCCCCHHHCCCCEEEEECCCCCCCCCHHHHHH
IVKSLQELEDDETLLVQSGKPVGVFKTHSQAPRVLIANSLLVPSWADWDQFRELEKQGLT
HHHHHHHCCCCCEEEEECCCCCEEEECCCCCCEEEEECCEECCCCCCHHHHHHHHHCCCE
MYGQMTAGSWIYIGTQGILQGTYETFGAAADKHYNGDLSGKFVLTAGLGGMGGAQPLAVT
EEEEECCCCEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEE
MNNGVVLCVEVDRERIERRIDYQYLDKMAENLDEALELVDEAVSEKKPLSIGLLGNAASI
ECCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHH
YPELVKRGRIPDMVTDQTSAHDILNGYVPADLDFPYALELRHQDPEKYMKLSKSSIAKHV
HHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
EAMLEFQKQGAIVFDYGNNIRQQAYIEGITKAFDFPGFVPAYIRPQFCEGKGPFRWVALS
HHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHCCCHHCCCCCCEEEEEEC
GDPEDIYRTDRLILEEFSDNEHLCRWIKMAGEQVSFQGLPSRICWLGYGERARFGKLIND
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEECCCCCCEEEEECCCCHHHHHHHHHH
LVAKGEIKAPIVIGRDHLDCGSVASPNRETEGMKDGSDAIADWPILNALINTAAGAHWVS
HHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
VHHGGGVGIGYSLHAGIVVMADGSKDAEERLERVLTSDPGTGIIRHVDAGYELAKKTASE
EECCCCCCCCEEECCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHH
QGVNIPMWS
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA