Click here to switch to the map view.

The map label for this gene is braB [H]

Identifier: 188587300

GI number: 188587300

Start: 2916572

End: 2917816

Strand: Reverse

Name: braB [H]

Synonym: Nther_2703

Alternate gene names: 188587300

Gene position: 2917816-2916572 (Counterclockwise)

Preceding gene: 188587303

Following gene: 188587299

Centisome position: 92.17

GC content: 37.27

Gene sequence:

>1245_bases
GTGATTTTTCCGCCATTTTTAGGTCATTCTTCTGGAAGTGACTGGTTGCCCGCTGCAGTTGGATTTTTGTTAACAGGAGT
AGGGCTTCCTCTGTTAGGGGTTATTGCAGCTTCGGTAGCCGGCGGCACTGTTGATGATTTTGGAAAAAATGTCAGCCCCA
ATTTCGGAAAAATTTTCGGAATAGTGATTGTTTTGGCAATTGGACCTTTATTAGCTATTCCCAGAACTGGAGCAACGGCT
TTTGAGCTAGGAGTTGTACCATTTTTCAGTGATTCACTTATTGCTTCAATAATTACTACAACTATCTTTTTTGGACTTAC
ATTATATTTTGTTCTCAATCCTTCTTCAGTTATAGATAGGATTGGAAAATACTTGACTTCAATCTTAGTTGCAATTCTGG
GAGCAATAGTTATAGGTGGGATTTTAAACCCCATAGGTAATCCCGTAGATATGGAGGCTGCTACACCCTTTAAAACTGGT
TTAACAGAAGGTTATCAAACTATGGATGCATTAGGTTCGGTTATTCTAGCGACCATCATAATTAGCAGTTTGAATGAAAA
AGGTTATAGTACCACATCAGATCAAGTTTCAATAACAATTAGAGCAGGAATGATTGCTGCTACAGGTTTAGGATTAATTT
ATGGTGGATTGCTGTTTCTAGGAGCAACTTCCAGTGCAGTTTTTTCTGAGGGGATCCATCGAACAGAATTATTAATTGGA
GTGAGCGATAAAATATTAGGCTCTATTGGTACTTATGCCTTGGGGATGAGTGTATTTTTAGCTTGCCTAACTACCTCAAT
AGGCCTAACAACCACAGCTGGCAAATTCTTTAGTGGAATTACAAATGGTAAATTAAATTATAAACTAGTAGTTTGGGTAA
CTGTTTTATTTAGTGCTTTTTTTGCCAATGCTGGAGTAGAATCTATTGTAAGGATAGCTGAGCCAATTTTGGTAGCAATA
TATCCTGTCGCCATTGTTCTGATAATTATGACAATCTTTAATAATTACATAAAAGATAAAGTGGTTTATCGGGGAGCTGT
TTTTGGAGCATTTATAGTTGGTTTATTTGACTCAGCCATGTTATTAGATTTAGAACTTTACGGACTCGAAAGTATTATGG
GGAATTTACCATTTGCAGAATACGGTGTTCAATGGATAGTACCTGCCATCTTGTTTGCCTTGATTTCTAAGTTACTCAAA
AGAGGGATTTCTCTACGGACTAAGGAGAGTACAGACACAGCTTAA

Upstream 100 bases:

>100_bases
TTTAAAAATCGTCATTTAGGAGGGTAATAATGAGTAAGAAAAGTTCTGACACAATCATTATCGGTTTCGCTCTTTTTGCC
ATGTTTTTAGGAGCAGGTAA

Downstream 100 bases:

>100_bases
ATTTACCAAAAATCAACAATCTGTTATAATTAACACAATATAAAATGAAACTCAGTTTAGATAACTTTTATGTAAAGGAT
GGGATCCAAAATGAGCGATA

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MIFPPFLGHSSGSDWLPAAVGFLLTGVGLPLLGVIAASVAGGTVDDFGKNVSPNFGKIFGIVIVLAIGPLLAIPRTGATA
FELGVVPFFSDSLIASIITTTIFFGLTLYFVLNPSSVIDRIGKYLTSILVAILGAIVIGGILNPIGNPVDMEAATPFKTG
LTEGYQTMDALGSVILATIIISSLNEKGYSTTSDQVSITIRAGMIAATGLGLIYGGLLFLGATSSAVFSEGIHRTELLIG
VSDKILGSIGTYALGMSVFLACLTTSIGLTTTAGKFFSGITNGKLNYKLVVWVTVLFSAFFANAGVESIVRIAEPILVAI
YPVAIVLIIMTIFNNYIKDKVVYRGAVFGAFIVGLFDSAMLLDLELYGLESIMGNLPFAEYGVQWIVPAILFALISKLLK
RGISLRTKESTDTA

Sequences:

>Translated_414_residues
MIFPPFLGHSSGSDWLPAAVGFLLTGVGLPLLGVIAASVAGGTVDDFGKNVSPNFGKIFGIVIVLAIGPLLAIPRTGATA
FELGVVPFFSDSLIASIITTTIFFGLTLYFVLNPSSVIDRIGKYLTSILVAILGAIVIGGILNPIGNPVDMEAATPFKTG
LTEGYQTMDALGSVILATIIISSLNEKGYSTTSDQVSITIRAGMIAATGLGLIYGGLLFLGATSSAVFSEGIHRTELLIG
VSDKILGSIGTYALGMSVFLACLTTSIGLTTTAGKFFSGITNGKLNYKLVVWVTVLFSAFFANAGVESIVRIAEPILVAI
YPVAIVLIIMTIFNNYIKDKVVYRGAVFGAFIVGLFDSAMLLDLELYGLESIMGNLPFAEYGVQWIVPAILFALISKLLK
RGISLRTKESTDTA
>Mature_414_residues
MIFPPFLGHSSGSDWLPAAVGFLLTGVGLPLLGVIAASVAGGTVDDFGKNVSPNFGKIFGIVIVLAIGPLLAIPRTGATA
FELGVVPFFSDSLIASIITTTIFFGLTLYFVLNPSSVIDRIGKYLTSILVAILGAIVIGGILNPIGNPVDMEAATPFKTG
LTEGYQTMDALGSVILATIIISSLNEKGYSTTSDQVSITIRAGMIAATGLGLIYGGLLFLGATSSAVFSEGIHRTELLIG
VSDKILGSIGTYALGMSVFLACLTTSIGLTTTAGKFFSGITNGKLNYKLVVWVTVLFSAFFANAGVESIVRIAEPILVAI
YPVAIVLIIMTIFNNYIKDKVVYRGAVFGAFIVGLFDSAMLLDLELYGLESIMGNLPFAEYGVQWIVPAILFALISKLLK
RGISLRTKESTDTA

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=419, Percent_Identity=28.6396181384248, Blast_Score=195, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 43452; Mature: 43452

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFPPFLGHSSGSDWLPAAVGFLLTGVGLPLLGVIAASVAGGTVDDFGKNVSPNFGKIFG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHH
IVIVLAIGPLLAIPRTGATAFELGVVPFFSDSLIASIITTTIFFGLTLYFVLNPSSVIDR
HHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
IGKYLTSILVAILGAIVIGGILNPIGNPVDMEAATPFKTGLTEGYQTMDALGSVILATII
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ISSLNEKGYSTTSDQVSITIRAGMIAATGLGLIYGGLLFLGATSSAVFSEGIHRTELLIG
HHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHEEE
VSDKILGSIGTYALGMSVFLACLTTSIGLTTTAGKFFSGITNGKLNYKLVVWVTVLFSAF
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
FANAGVESIVRIAEPILVAIYPVAIVLIIMTIFNNYIKDKVVYRGAVFGAFIVGLFDSAM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LLDLELYGLESIMGNLPFAEYGVQWIVPAILFALISKLLKRGISLRTKESTDTA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
>Mature Secondary Structure
MIFPPFLGHSSGSDWLPAAVGFLLTGVGLPLLGVIAASVAGGTVDDFGKNVSPNFGKIFG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHH
IVIVLAIGPLLAIPRTGATAFELGVVPFFSDSLIASIITTTIFFGLTLYFVLNPSSVIDR
HHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
IGKYLTSILVAILGAIVIGGILNPIGNPVDMEAATPFKTGLTEGYQTMDALGSVILATII
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ISSLNEKGYSTTSDQVSITIRAGMIAATGLGLIYGGLLFLGATSSAVFSEGIHRTELLIG
HHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHEEE
VSDKILGSIGTYALGMSVFLACLTTSIGLTTTAGKFFSGITNGKLNYKLVVWVTVLFSAF
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
FANAGVESIVRIAEPILVAIYPVAIVLIIMTIFNNYIKDKVVYRGAVFGAFIVGLFDSAM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LLDLELYGLESIMGNLPFAEYGVQWIVPAILFALISKLLKRGISLRTKESTDTA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]