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The map label for this gene is opuE [H]

Identifier: 188587171

GI number: 188587171

Start: 2758497

End: 2759891

Strand: Reverse

Name: opuE [H]

Synonym: Nther_2570

Alternate gene names: 188587171

Gene position: 2759891-2758497 (Counterclockwise)

Preceding gene: 188587172

Following gene: 188587170

Centisome position: 87.19

GC content: 41.86

Gene sequence:

>1395_bases
ATGTATGGTGCAATACTGGCCGTTTATGTTTTGATAATCTTGGGAATTGGGTTTTACACATCGCGCTACATCAAAAACAT
GGATGACTTCGCCTTGGCCGGAAGAAATTTAGGACTTCCCATGTTGGTTGGAACCCTTTTCGCTTCATTTGTGGGTGGTG
CAACCGTTACAGGTTGGACAGGCAGTTTCTATCAATTGGGCTTTGACTGGTGGTTTGCCGGTATCGGTTCACTATTAGGT
GTAGCAGTGGCTGCTACTGTAATGGCCGAAAAAAGTAGGAAACTAGAACAGTACACAGTCCCTGACCTTCTTGAGTTAAG
ATACGATAACAAAACTAAAGTTGTAGGCTCCATCATGATCATTTTAGGCGATATAGGTTTGGTAGCCGTCCAAATTATCG
CCTTAAGCGGAATATTAACAGCTTTTGTTGGACTTGAAACTATGCCAGCTATGATACTTAGTATAATAGTCTTTACTTTA
ATTGCACTATTTGGCGGTATGGTCGGTGTAGCTTTGACCGACAGTATACAGGCCATAATTATCGCTTTAGGTCTAATCGT
AGGAGTGATATCTGCATTTAGTTATGGTGGAGGTATAGGTGCTGTTTTCTCGGACTTACCTGATAATTTCCTAACTCCAC
TAGGGACTACTTCTCCCGGTGAGGCCTTTAACAATGCCATCGCCGTATTCGGAACTACTGCCGTGTGGCAGGCCATAATT
TTCTCCAGGGCTTTTGCCGCCAAAGACAGCAGAACAGCTAAAATGGGGATACTATGGTTGATTCCTACATCATTAATAGG
TTACTTTTTAGTAGCTATGTTAGGTTATAGTGCTAGAAGTATTTTGGGTCCGGATGTAGTGCCTGACGAAGTTTTTGCGA
CTTTACTTTCTGAGGTTATGAATCCTGCCGTAGCTGCTATATTACTGGCAGTGATTGTAGGTGCCATCATTACTTCCACT
AATTCATTCCTTCTAAGTATCAGCATCAATATCACAAGGGATCTTTATCAAAATTTCATTGGAAAAGACAGCAGTGACAA
GAGTTTACTCAAGGTGGGCAGAGTATCTCTTGTGATTGTGGCAGCACTGGCATTTTCATTAGCTGTAGCCATGCCCGATA
TCGTAACAGCCATAGTGTTTGCATATACAATGTACAGCGCAGCTCTGTTAATACCACTTTATGGTGGCTATCTATGGAAA
AAGGCCAACTCTACTGCCGGATTTTTAGGGATAATATTTGGCGGTGGAACAGCTGTTATTTGGCATTTACTTAATGAACC
TATGGGACTTCCCCCTATGGTGCCGGCAGTAATATTATCTTTAATAGTTTTTGTAGCTTCTAGTTACATGACAGAGGGGC
CGTCTAAAGAACAGTTAAAGGTGTTTGATGTATAA

Upstream 100 bases:

>100_bases
GTTTTTTAACCCTGGAGATGAAGAGTCCATCAGCGTATGGGCCATGACCCCGCCTTCTTTCTGATAAATTAACCAAATTT
TACATAGGGGGTAAAAATTG

Downstream 100 bases:

>100_bases
TTGCTTGAGCTAAGCAGACTGACTTGGCAAGAAATTCAAGATAAACTGCCGGACCTGGATGTATTGTTACTTCCTGTAGG
ATCTATAGAACAACACGGCC

Product: Na+/solute symporter

Products: Na (I) [Cytoplasm]; pantothenate [Cytoplasm] [C]

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MYGAILAVYVLIILGIGFYTSRYIKNMDDFALAGRNLGLPMLVGTLFASFVGGATVTGWTGSFYQLGFDWWFAGIGSLLG
VAVAATVMAEKSRKLEQYTVPDLLELRYDNKTKVVGSIMIILGDIGLVAVQIIALSGILTAFVGLETMPAMILSIIVFTL
IALFGGMVGVALTDSIQAIIIALGLIVGVISAFSYGGGIGAVFSDLPDNFLTPLGTTSPGEAFNNAIAVFGTTAVWQAII
FSRAFAAKDSRTAKMGILWLIPTSLIGYFLVAMLGYSARSILGPDVVPDEVFATLLSEVMNPAVAAILLAVIVGAIITST
NSFLLSISINITRDLYQNFIGKDSSDKSLLKVGRVSLVIVAALAFSLAVAMPDIVTAIVFAYTMYSAALLIPLYGGYLWK
KANSTAGFLGIIFGGGTAVIWHLLNEPMGLPPMVPAVILSLIVFVASSYMTEGPSKEQLKVFDV

Sequences:

>Translated_464_residues
MYGAILAVYVLIILGIGFYTSRYIKNMDDFALAGRNLGLPMLVGTLFASFVGGATVTGWTGSFYQLGFDWWFAGIGSLLG
VAVAATVMAEKSRKLEQYTVPDLLELRYDNKTKVVGSIMIILGDIGLVAVQIIALSGILTAFVGLETMPAMILSIIVFTL
IALFGGMVGVALTDSIQAIIIALGLIVGVISAFSYGGGIGAVFSDLPDNFLTPLGTTSPGEAFNNAIAVFGTTAVWQAII
FSRAFAAKDSRTAKMGILWLIPTSLIGYFLVAMLGYSARSILGPDVVPDEVFATLLSEVMNPAVAAILLAVIVGAIITST
NSFLLSISINITRDLYQNFIGKDSSDKSLLKVGRVSLVIVAALAFSLAVAMPDIVTAIVFAYTMYSAALLIPLYGGYLWK
KANSTAGFLGIIFGGGTAVIWHLLNEPMGLPPMVPAVILSLIVFVASSYMTEGPSKEQLKVFDV
>Mature_464_residues
MYGAILAVYVLIILGIGFYTSRYIKNMDDFALAGRNLGLPMLVGTLFASFVGGATVTGWTGSFYQLGFDWWFAGIGSLLG
VAVAATVMAEKSRKLEQYTVPDLLELRYDNKTKVVGSIMIILGDIGLVAVQIIALSGILTAFVGLETMPAMILSIIVFTL
IALFGGMVGVALTDSIQAIIIALGLIVGVISAFSYGGGIGAVFSDLPDNFLTPLGTTSPGEAFNNAIAVFGTTAVWQAII
FSRAFAAKDSRTAKMGILWLIPTSLIGYFLVAMLGYSARSILGPDVVPDEVFATLLSEVMNPAVAAILLAVIVGAIITST
NSFLLSISINITRDLYQNFIGKDSSDKSLLKVGRVSLVIVAALAFSLAVAMPDIVTAIVFAYTMYSAALLIPLYGGYLWK
KANSTAGFLGIIFGGGTAVIWHLLNEPMGLPPMVPAVILSLIVFVASSYMTEGPSKEQLKVFDV

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=490, Percent_Identity=27.3469387755102, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI109659836, Length=541, Percent_Identity=23.8447319778189, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI11141885, Length=392, Percent_Identity=25, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI109659839, Length=557, Percent_Identity=24.0574506283662, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI256985183, Length=348, Percent_Identity=20.9770114942529, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI87082237, Length=480, Percent_Identity=26.875, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1787251, Length=481, Percent_Identity=25.987525987526, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790503, Length=508, Percent_Identity=22.4409448818898, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790113, Length=353, Percent_Identity=24.3626062322946, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17539284, Length=404, Percent_Identity=24.5049504950495, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648033, Length=389, Percent_Identity=25.706940874036, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21356865, Length=389, Percent_Identity=25.706940874036, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24641117, Length=270, Percent_Identity=24.8148148148148, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 49034; Mature: 49034

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
>Mature Secondary Structure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HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; pantothenate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + pantothenate [Periplasm] = Na (I) [Cytoplasm] + pantothenate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]