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The map label for this gene is mdeA [H]

Identifier: 188587063

GI number: 188587063

Start: 2637374

End: 2638552

Strand: Reverse

Name: mdeA [H]

Synonym: Nther_2455

Alternate gene names: 188587063

Gene position: 2638552-2637374 (Counterclockwise)

Preceding gene: 188587067

Following gene: 188587062

Centisome position: 83.35

GC content: 39.78

Gene sequence:

>1179_bases
ATGAAAGATTCAAAATTAACTACTCGTATTGTCCATGCCGATGAAGGTCAAAAGAACGAAGCGGGTGCTCTTAGGACCCC
AATTTATCAAAGTTCAACCTTTGTATTTGACAATGTTGAACAAGGAGCTGCTAGATTTGCCGGTGAAGAGGCTGGCTACA
TCTACACTAGATTGGGTAACCCAACTCAAAAAAATCTTGAAAAGCAGATGGCTAACCTAGAAGGTGGAGATGATGCTATA
GCATTAGGATCTGGTATGGCAGCTGTGTCTGCTGTTGTGATGAATTTATTAAATTGTGGAGATCATATGGTGGCCTCAGA
TACACTATATGGCTGTACTCACTCTCTGTTTGACGAGTTGTTACCCAACTGGGGCATTGAAGTAACTTTTGTTGATACCA
GTGAGCCTGAAAATATCCGAGCTGCTATTAAAGATAATACAAAGGTGATTTATACTGAAACTCCTGCGAATCCAACTATT
TCCATCACTGATCTGGAAGAAGTTGTGAAAATTGCAAAGGAAAAGGACGACATTACAACTGTAGTAGACAACACCTTTAT
GACTCCTTATCTTCAAAGACCGCTAGAAATGGGAATTGATATCGTAATTCATAGTGCTACCAAGTACCTTGGTGGTCACG
GAGATACTGTTGCCGGAATAGTAGTAGGTTTCGAGGAACTGATTGGTGATATAAAAATGACTACAGTAAAAGATTTAGGT
GGTATTATTGCACCTTTTGATGCTTTCCTATTGCTCAGAGGAATTAAAACTTTAGCTGTTCGTGTGGACAAGATCTGTGA
AAACGCTATGAAGGTAGCCAAGTTCTTAGAAGAACATCCAGCTGTAGAAAGAGTGTACTACCCAGGACTTGAAAGCCATC
CTCAACACGAGTTAGCTAAAAAACAAATGGAAGACTTTGGTGGCATTATTGCCTTTGAATTAAAAGGTGGCTTTGAAGCT
GGTAAAAACCTACTAAATAATGTAGAGTTGTGTACATTGGCTGTTAGCTTGGGAGATGTGGATACATTGATCCAGCATCC
AGCATCCATGACTCATTCTGTAGTTCCACAAGAAGAACGTGAAAAAGCAGGTATTACCGATGGTATGGTAAGGGTTTCCG
TAGGAATTGAAGATGTAGATGATATTATCGAAGACCTAAAACAAGGAATGGAACAGTAA

Upstream 100 bases:

>100_bases
TATAGGGAAGTAGAGCCAATGGTTTGCCGAATTAATTGAAGCTGGCACAGTTATTGCATATAAATAGATGACACAAATAC
AATAAGTGGAGGTGTTTTTA

Downstream 100 bases:

>100_bases
TTTAGAAAGAAACCCTTAGAAATATCATCAAACTACAAAACCAAGGGGCTGAGGGATAATTATTTATTGCAATTCCCTCA
GCGCCTCCCTACATTACATT

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKDSKLTTRIVHADEGQKNEAGALRTPIYQSSTFVFDNVEQGAARFAGEEAGYIYTRLGNPTQKNLEKQMANLEGGDDAI
ALGSGMAAVSAVVMNLLNCGDHMVASDTLYGCTHSLFDELLPNWGIEVTFVDTSEPENIRAAIKDNTKVIYTETPANPTI
SITDLEEVVKIAKEKDDITTVVDNTFMTPYLQRPLEMGIDIVIHSATKYLGGHGDTVAGIVVGFEELIGDIKMTTVKDLG
GIIAPFDAFLLLRGIKTLAVRVDKICENAMKVAKFLEEHPAVERVYYPGLESHPQHELAKKQMEDFGGIIAFELKGGFEA
GKNLLNNVELCTLAVSLGDVDTLIQHPASMTHSVVPQEEREKAGITDGMVRVSVGIEDVDDIIEDLKQGMEQ

Sequences:

>Translated_392_residues
MKDSKLTTRIVHADEGQKNEAGALRTPIYQSSTFVFDNVEQGAARFAGEEAGYIYTRLGNPTQKNLEKQMANLEGGDDAI
ALGSGMAAVSAVVMNLLNCGDHMVASDTLYGCTHSLFDELLPNWGIEVTFVDTSEPENIRAAIKDNTKVIYTETPANPTI
SITDLEEVVKIAKEKDDITTVVDNTFMTPYLQRPLEMGIDIVIHSATKYLGGHGDTVAGIVVGFEELIGDIKMTTVKDLG
GIIAPFDAFLLLRGIKTLAVRVDKICENAMKVAKFLEEHPAVERVYYPGLESHPQHELAKKQMEDFGGIIAFELKGGFEA
GKNLLNNVELCTLAVSLGDVDTLIQHPASMTHSVVPQEEREKAGITDGMVRVSVGIEDVDDIIEDLKQGMEQ
>Mature_392_residues
MKDSKLTTRIVHADEGQKNEAGALRTPIYQSSTFVFDNVEQGAARFAGEEAGYIYTRLGNPTQKNLEKQMANLEGGDDAI
ALGSGMAAVSAVVMNLLNCGDHMVASDTLYGCTHSLFDELLPNWGIEVTFVDTSEPENIRAAIKDNTKVIYTETPANPTI
SITDLEEVVKIAKEKDDITTVVDNTFMTPYLQRPLEMGIDIVIHSATKYLGGHGDTVAGIVVGFEELIGDIKMTTVKDLG
GIIAPFDAFLLLRGIKTLAVRVDKICENAMKVAKFLEEHPAVERVYYPGLESHPQHELAKKQMEDFGGIIAFELKGGFEA
GKNLLNNVELCTLAVSLGDVDTLIQHPASMTHSVVPQEEREKAGITDGMVRVSVGIEDVDDIIEDLKQGMEQ

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=374, Percent_Identity=42.2459893048128, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI299473759, Length=374, Percent_Identity=39.3048128342246, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI25453487, Length=374, Percent_Identity=36.6310160427808, Blast_Score=226, Evalue=3e-59,
Organism=Escherichia coli, GI1790375, Length=372, Percent_Identity=37.3655913978495, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1789383, Length=402, Percent_Identity=31.0945273631841, Blast_Score=198, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI71980708, Length=392, Percent_Identity=41.8367346938776, Blast_Score=304, Evalue=6e-83,
Organism=Caenorhabditis elegans, GI17560056, Length=353, Percent_Identity=44.1926345609065, Blast_Score=296, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17538055, Length=369, Percent_Identity=42.2764227642276, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI193202265, Length=109, Percent_Identity=34.8623853211009, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319307, Length=394, Percent_Identity=39.3401015228426, Blast_Score=280, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6323333, Length=438, Percent_Identity=36.3013698630137, Blast_Score=246, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6321254, Length=414, Percent_Identity=31.1594202898551, Blast_Score=182, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6321170, Length=338, Percent_Identity=31.0650887573964, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6321904, Length=400, Percent_Identity=29.25, Blast_Score=143, Evalue=6e-35,
Organism=Drosophila melanogaster, GI19922554, Length=395, Percent_Identity=39.4936708860759, Blast_Score=300, Evalue=9e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 42716; Mature: 42716

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSKLTTRIVHADEGQKNEAGALRTPIYQSSTFVFDNVEQGAARFAGEEAGYIYTRLGN
CCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCCCEEEEECCC
PTQKNLEKQMANLEGGDDAIALGSGMAAVSAVVMNLLNCGDHMVASDTLYGCTHSLFDEL
CHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHH
LPNWGIEVTFVDTSEPENIRAAIKDNTKVIYTETPANPTISITDLEEVVKIAKEKDDITT
CCCCCEEEEEECCCCCCHHHHEECCCCEEEEEECCCCCCEEECCHHHHHHHHHCCCCHHH
VVDNTFMTPYLQRPLEMGIDIVIHSATKYLGGHGDTVAGIVVGFEELIGDIKMTTVKDLG
HHCCCCCCHHHHCHHHCCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHC
GIIAPFDAFLLLRGIKTLAVRVDKICENAMKVAKFLEEHPAVERVYYPGLESHPQHELAK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCHHHHHH
KQMEDFGGIIAFELKGGFEAGKNLLNNVELCTLAVSLGDVDTLIQHPASMTHSVVPQEER
HHHHHHCCEEEEEECCCHHHHHHHHCCCCEEHEEEHHCCHHHHHHCCHHHHHHCCCCHHH
EKAGITDGMVRVSVGIEDVDDIIEDLKQGMEQ
HHCCCCCCEEEEEECHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDSKLTTRIVHADEGQKNEAGALRTPIYQSSTFVFDNVEQGAARFAGEEAGYIYTRLGN
CCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCCCEEEEECCC
PTQKNLEKQMANLEGGDDAIALGSGMAAVSAVVMNLLNCGDHMVASDTLYGCTHSLFDEL
CHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHH
LPNWGIEVTFVDTSEPENIRAAIKDNTKVIYTETPANPTISITDLEEVVKIAKEKDDITT
CCCCCEEEEEECCCCCCHHHHEECCCCEEEEEECCCCCCEEECCHHHHHHHHHCCCCHHH
VVDNTFMTPYLQRPLEMGIDIVIHSATKYLGGHGDTVAGIVVGFEELIGDIKMTTVKDLG
HHCCCCCCHHHHCHHHCCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHC
GIIAPFDAFLLLRGIKTLAVRVDKICENAMKVAKFLEEHPAVERVYYPGLESHPQHELAK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCHHHHHH
KQMEDFGGIIAFELKGGFEAGKNLLNNVELCTLAVSLGDVDTLIQHPASMTHSVVPQEER
HHHHHHCCEEEEEECCCHHHHHHHHCCCCEEHEEEHHCCHHHHHHCCHHHHHHCCCCHHH
EKAGITDGMVRVSVGIEDVDDIIEDLKQGMEQ
HHCCCCCCEEEEEECHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]